Abstract:
:Raman spectra were obtained from fresh, fixed and sliced rabbit lenses and from human lens slices. For all lenses and lens slices the ratio R, defined as the Raman intensity at 3390 cm-1 divided by the Raman intensity at 2935 cm-1, was measured at different locations along the visual and equatorial axis. The ratios R were transformed to absolute water mass percentages by measuring solutions with known protein concentrations. It was shown that fixation and slicing have very little effect on the absolute water content of the lenses. The values obtained for the absolute water content are comparable to values given in literature. It was also shown that the water content in rabbit and human lenses rapidly decreases from the immediate anterior and posterior subsurface region to the deep superficial cortex and is relatively constant in the nucleus. Raman microspectroscopy appears to be a reliable method for the measurement of the absolute water content of small volumes on defined positions in the lens. This can be very useful when analyzing the possible relation between local variations in water content and the occurrence of opacities in the lens.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Huizinga A,Bot AC,de Mul FF,Vrensen GF,Greve Jdoi
10.1016/0014-4835(89)90032-8subject
Has Abstractpub_date
1989-04-01 00:00:00pages
487-96issue
4eissn
0014-4835issn
1096-0007journal_volume
48pub_type
杂志文章abstract::Multiple species of mucins are synthesized and secreted by corneal and conjunctival epithelial cells. These mucins are vital components of the tear film protecting the ocular surface from the external environment by providing a physical and chemical barrier. The release of mucins must be tightly regulated as both muci...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2004-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90093-6
更新日期:1989-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0297
更新日期:1997-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.10.019
更新日期:2014-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2015.05.024
更新日期:2015-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1999.0677
更新日期:1999-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80047-7
更新日期:1987-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.01.014
更新日期:2006-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.10.003
更新日期:2010-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.06.001
更新日期:2011-10-01 00:00:00
abstract::Nitric oxide (NO) production by vascular endothelium is important in regulation of blood flow. Reduced production of NO can adversely affect blood flow and other vascular functions. We investigated the expression of three nitric oxide synthase (NOS) isoforms in retina and choroid of aged human eyes and eyes with AMD. ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.10.004
更新日期:2010-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.10.006
更新日期:2005-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.05.011
更新日期:2012-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0101
更新日期:1996-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.06.006
更新日期:2006-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.06.008
更新日期:2018-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.03.013
更新日期:2006-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.12.013
更新日期:2005-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90075-7
更新日期:1984-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2013-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0936
更新日期:2001-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.01.004
更新日期:2010-05-01 00:00:00
abstract::Selective pericyte loss, the histological hallmark of early diabetic retinopathy (DR), enhances the breakdown of the blood-retinal barrier (BRB) in diabetes. However, the role of pericytes on BRB alteration in diabetes and the signaling pathways involved in their effects are currently unknown. To understand the role o...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108043
更新日期:2020-06-01 00:00:00
abstract:PURPOSE:To assess the inter-ocular and inter-session reliability for a range of parameters derived from the photopic electroretinogram (ERG) in a group of normal non-human primates. METHODS:Inter-ocular differences for photopic ERGs were assessed in a group of normal anesthetized adult rhesus monkeys (Macaca mulatta, ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2003.09.013
更新日期:2004-01-01 00:00:00
abstract::In order to determine whether neuronal cell death in the retina accompanies senescence, retinas from pigmented ACI rats of various ages were analysed for changes in dimensions, cell densities, DNA content, and rhodopsin levels. Between the ages of 4 and 30 months, there were significant declines in the densities of nu...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90022-9
更新日期:1986-04-01 00:00:00
abstract::Human, monkey, and bovine retinal pigment epithelial (RPE) cells exhibit an M-type K+ current, which in many other cell types is mediated by channels composed of KCNQ α-subunits and KCNE auxiliary subunits. Recently, we demonstrated the expression of KCNQ1, KCNQ4, and KCNQ5 in the monkey RPE. Here, we investigated the...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:
更新日期:2013-11-01 00:00:00
abstract::A shift or displacement of the retinal blood vessels (RBVs) with neuroretinal rim thinning indicates the progression of glaucomatous optic neuropathy. In chronic open angle glaucoma, individuals with RBV positional shifts exhibit more rapid visual field loss than those without RBV shifts. The retinal vessels reportedl...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.07.012
更新日期:2017-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.07.009
更新日期:2007-10-01 00:00:00
abstract::Female New Zealand White rabbits were treated subcutaneously with 0.0375 or 0.15 mg kg-1 dexamethasone or physiological saline solution from the 8th through to the 11th day of gestation. Short-term administration of 0.15 mg kg-1 dexamethasone induced two types of morphological lens deviations in the offspring (lenses ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90008-3
更新日期:1984-08-01 00:00:00