Abstract:
:Limbal transplants in humans show a high rate of rejection even under local and systemic immunotherapy. In order to test immunomodulatory treatments a new limbal transplant model in the rat was developed using enhanced green fluorescent protein (E-GFP) as marker for follow-up. Sixty E-GFP-positive limbal transplants from Sprague-Dawley TgN(act-EGFP)Osb4 rats were transplanted onto 18 wild-type inbred Sprague-Dawley (isografts) rats, six wild-type litter mate Sprague-Dawley (sibling) rats, 18 Fischer 344 (allografts) rats, and 18 Fischer 344 rats depleted from monocytes and macrophages by subconjunctival treatment with clodronate liposomes. All rats were monitored three times a week with fluorescence microscopy, until fluorescence had disappeared. At postoperative days 6, 9, 12, and 15, three rats of all groups were killed for immunohistochemical analysis of infiltrating cells. Using a modified digital fluorescence microscope, we were able to monitor transplant behavior over time without disturbance of the ocular surface. The average days of rejection were 14 days in the isograft group, the sibling group, and the untreated allograft group. However, the average day of rejection in the allogeneic macrophage-depleted group was 27 days. Marked infiltration of macrophages and lymphocytes was seen in the untreated isografts and allografts. In the clodronate liposome-treated allografts infiltration was minor. A successful new limbal transplant model is described. The transplant can be accurately followed up in vivo by E-GFP labeling of the donor tissue without disturbing the corneal surface. Although E-GFP itself proved to be immunogenic, local clodronate liposome injections significantly increased graft survival. So the model seems to be useful for testing immunosuppressive or modulatory agents in limbal transplantation studies.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Keijser S,de Keizer RJ,Prins FA,Tanke HJ,van Rooijen N,Vrensen GF,Jager MJdoi
10.1016/j.exer.2006.06.006subject
Has Abstractpub_date
2006-11-01 00:00:00pages
1188-95issue
5eissn
0014-4835issn
1096-0007pii
S0014-4835(06)00295-8journal_volume
83pub_type
杂志文章abstract::Early stages of glaucoma and optic neuropathies are thought to show inner retina remodeling and functional changes of retinal ganglion cells (RGCs) before they die. To assess RGC functional plasticity, we investigated the contrast-gain control properties of the pattern electroretinogram (PERG), a sensitive measure of ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.11.008
更新日期:2016-04-01 00:00:00
abstract::Exposure to hyposmotic solution causes release of ATP from lens cells via hemichannels. Because hemichannel opening feasibly could swamp the cells with calcium, we carried out studies to measure the magnitude of the increase in cytoplasmic calcium concentration caused by hemichannel opening. In studies on porcine lens...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.01.012
更新日期:2015-03-01 00:00:00
abstract::Ischemic preconditioning (IPC) protects the rat retina against the injury that ordinarily follows severe ischemia. We showed previously that release of adenosine and de novo protein synthesis were required for IPC protection. The mechanisms of IPC were studied in the rat retina by examining the signal transduction med...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0843
更新日期:2000-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(83)90026-x
更新日期:1983-11-01 00:00:00
abstract::Adipose-Derived Stem Cells (ADSCs) have an important contribution in regenerative medicine ranging from testing stem cell therapy for disease treatment in pre-clinical models to clinical trials. For immediate use of stem cells for therapy, there is a requirement of the high dose of stem cells at different time points ...
journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.04.007
更新日期:2013-07-01 00:00:00
abstract::The CLN2 form of neuronal ceroid lipofuscinosis is an autosomal recessively inherited lysosomal storage disease that is characterized by progressive vision loss culminating in blindness, cognitive and motor decline, neurodegeneration, and premature death. CLN2 disease results from mutations in the gene that encodes th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.02.012
更新日期:2015-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.05.020
更新日期:2015-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.06.025
更新日期:2018-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(02)00248-8
更新日期:2003-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.02.020
更新日期:2009-06-15 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.05.011
更新日期:2007-10-01 00:00:00
abstract::Experimental autoimmune uveoretinitis (EAU) in mice provides a useful platform to study the pathogenesis and experimental therapeutics of human uveitis. One often used EAU model employs C57BL/6 (B6) mice sensitized with a peptide residue having 1 to 20 amino acids of human interphotoreceptor retinoid binding protein (...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2020-12-19 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80113-6
更新日期:1987-11-01 00:00:00
abstract::Scanning electron microscopy (SEM) of mouse cornea and conjunctiva fixed with picric acid-paraformaldehyde-glutaraldehyde (PA-P-G) mixture revealed a thin layer of amorphous material covering the microvilli of the corneal surface cells. At the transmission electron microscopic (TEM) level, this layer of material stain...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90111-8
更新日期:1984-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0379
更新日期:1997-11-01 00:00:00
abstract::The in vitro and in vivo effects of a new immunomodulating agent, bucillamine, on experimental autoimmune uveitis (EAU) was studied in the rat. The capacity of S-antigen-sensitized lymphocytes to proliferate in response to the antigen or to produce antigen-specific antibodies was significantly suppressed by bucillamin...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1036
更新日期:1994-04-01 00:00:00
abstract::Retinal inflammation is an integral component of many retinal diseases including diabetic retinopathy (DR), age-related macular degeneration (AMD) and retinopathy of prematurity (ROP). Inflammation is commonly initiated and perpetuated by myeloid-derived immune cells. In the retina, microglial cells are resident macro...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.05.010
更新日期:2019-08-01 00:00:00
abstract::Intraocular pressure (IOP) is an important risk factor for glaucoma, and the response of the ONH and surrounding tissues to elevated IOP are often investigated to better understand pathophysiology. In vivo structure including that of the optic nerve head (ONH) and surrounding tissue of the eye are often assessed using...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.01.025
更新日期:2018-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2017-11-01 00:00:00
abstract::Glaucoma challenges the survival of retinal ganglion cell axons in the optic nerve through processes dependent on both aging and ocular pressure. Relevant stressors likely include complex interplay between axons and astrocytes, both in the retina and optic nerve. In the DBA/2J mouse model of pigmentary glaucoma, early...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.11.016
更新日期:2016-09-01 00:00:00
abstract::Reactive astrocytes in glaucomatous optic nerve changes are characterized by an increased expression of alphaB-crystallin and transforming growth factor-beta (TGF-beta). In the pathogenesis of glaucomatous optic nerve damage, ischemia/reperfusion injury may play an important role. The goal of the present study was to ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.12.008
更新日期:2007-04-01 00:00:00
abstract::Crystallin-bound non-tryptophan fluorescence and protein cross-linking were studied in chronic galactosemia. Fluorescence (excitation/emission--370/440 nm) was significantly higher in galactosemic rats compared to controls (P less than 0.001). Accumulation of fluorescence was significantly reduced both in water solubl...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90153-l
更新日期:1990-10-01 00:00:00
abstract::The mechanisms by which sulfhydryl (SH-) binding reagents modulate the light-sensitive conductance of retinal rods were investigated by current recording from single rods, by patch clamp recording from the plasma membrane of the rod outer segment (ROS), and by biochemical study of their effects on the light-induced hy...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90176-u
更新日期:1990-07-01 00:00:00
abstract::Forskolin, a diterpene, a potent and direct stimulator of the catalytic unit of adenylate cyclase, can reduce intraocular pressure when administered topically to rabbit eyes in a 1% suspension. Using an intravitreal plant of fluorescein-dextran, aqueous flow was measured by a modification of the method of Maurice. For...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90113-1
更新日期:1984-07-01 00:00:00
abstract::Iron is an essential biometal in the aqueous humor, the principal source of nutrients for the avascular cornea and the lens. Here, we explored whether the ciliary body (CB), the source of aqueous humor, transports iron, and if the prion protein (PrPC) facilitates this process as in the outer retina. Using a combinatio...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.05.031
更新日期:2018-10-01 00:00:00
abstract::Myopia (short-sightedness) is a visual problem associated with excessive eye growth and vitreous chamber expansion. Within the eye serotonin (5-hydroxytryptamine, 5-HT) appears to have a variety of effects, it alters retinal amacrine cell processing, increases intraocular pressure, constricts ocular blood vessels, and...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.03.021
更新日期:2005-11-01 00:00:00
abstract::Omental cells (OCs) are shown to help wound healing. The purpose of this study is to investigate if OCs improve cornea repair after alkali injury by subconjunctival injection of activated OCs in rats. Forty eight hours after limbal corneal alkali injury, fresh isolated OCs were injected subconjunctivally into the reci...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.02.014
更新日期:2014-04-01 00:00:00
abstract::Nerve growth factor in the tear film and corneal epithelium is hypothesized to play an important role in ocular surface maintenance and corneal wound healing. The purpose of this study was to determine the expression of nerve growth factor and its high affinity (trkA) receptor in tears, cornea, and lacrimal glands of ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.11.013
更新日期:2005-05-01 00:00:00
abstract::The DBA/2 mouse has been used as a model for spontaneous secondary glaucoma. We attempted to determine the in vivo time course and spatial distribution of retinal ganglion cells (RGCs) undergoing apoptotic death in DBA/2 mice. Female DBA/2 mice, 3, 9-10, 12, 15, and 18 months of age, received intravitreal injections o...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.08.009
更新日期:2007-01-01 00:00:00