Abstract：
:The carbohydrate epitope Gal alpha 1-3Gal-R (alpha-galactosyl epitope), which is detectable by its binding with Bandeiraea simplicifolia-IB4 lectin, was found in glycosphingolipids (GSLs), both neutral and acidic (gangliosides), from lens tissues of non-primate mammals, but not in those of human senile cataracts and Old World monkeys. Instead, human cataractous and Old World monkey non-cataractous lenses expressed Lewisx (Le(x)) epitopes (Gal beta 1-4(Fuc alpha 1-3)GlcNAc-R) in neutral GSLs. Sialylated Le(x) epitopes were found in rat and pig lenses as well as in human and Old World monkey lenses. Ganglio-series gangliosides, consisting mainly of GM3, GM1, GD1a and GD3, were detected in a species-specific fashion. On the other hand, alpha-galactosyl epitopes were expressed in lens tissues only in water-insoluble proteins of non-primate mammals, but Le(x) and sialylated Le(x) epitopes were not detectable in lens proteins. Among the several mammalian lenses examined, humans and Old World monkeys showed similar GSL compositions, in particular the presence of Le(x) and sialylated Le(x) epitopes and the absence of alpha-galactosyl epitopes, in lens tissue.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Ogiso M,Okinaga T,Komoto M,Nishiyama I,Hoshi Mdoi
10.1006/exer.1994.1151subject
Has Abstractpub_date
1994-12-01 00:00:00pages
653-63issue
6eissn
0014-4835issn
1096-0007pii
S0014-4835(84)71151-1journal_volume
59pub_type
杂志文章abstract：:A micropuncture technique involving the use of microcannulas with tip diameters less than 5 microns was used to measure the pressure in Schlemm's canal and in the meshwork at distances approximately 7 and 14 microns from the inner wall of Schlemm's canal. In one set of experiments where the spontaneous intraocular pre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90151-h
更新日期：1992-06-01 00:00:00
abstract：:Mice are now routinely utilized in studies of aqueous humor outflow dynamics. In particular, conventional aqueous outflow facility (C) is routinely measured via perfusion of the aqueous chamber by a number of laboratories. However, in mouse eyes perfused ex-vivo, values for C are variable depending upon whether the pe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.08.011
更新日期：2017-11-01 00:00:00
abstract：:Colchicine, a naturally occurring alkaloid, inhibits both fast and slow axoplasmic transport by prevention of microtubule assembly. We have examined the influence of colchicine on the neurogenically mediated acute inflammatory responses to topically administered nitrogen mustard and formaldehyde on the albino rabbit e...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90071-x
更新日期：1984-12-01 00:00:00
abstract：:A proteinase from human lens membrane was purified by a procedure previously developed for a similar proteinase from bovine lenses (Srivastava, 1988a). The purification of the human proteinase was achieved by solubilization of the enzyme from membranes with 2% sodium deoxycholate followed by two consecutive passages t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80067-3
更新日期：1989-02-01 00:00:00
abstract：:The passage of glucose within the cornea has been thought to occur by passive diffusion processes. However, corneal glucose concentration profiles have not been established to support this notion. While microfluorometric methods of metabolite assay typically have been used as a means of assessing regional brain metabo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(88)90037-1
更新日期：1988-11-01 00:00:00
abstract：:The response of the lens to changes in the distribution and concentration of electrolytes can be measured by characterizing the changes in resistivity that occur secondary to variations in intracellular composition. Regional intracellular cytoplasmic resistivities of lens fiber cells can be approximated by homogenates...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80164-1
更新日期：1987-04-01 00:00:00
abstract：:Corneal endothelium appears to be an important target tissue for retinoids and epidermal growth factor (EGF). We report here that retinoic acid, or its synthetic analogue CBS-211 A (10(-8)-10(-7) M) dose-dependently enhances the mitogenic effect of EGF on cultured bovine corneal endothelial cells (BCEC). Furthermore, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90169-u
更新日期：1990-07-01 00:00:00
abstract：:The lacrimal glands of male NOD mice exhibit many of the features of the human lacrimal gland in patients afflicted with the autoimmune disease, Sjögren's syndrome, including loss of secretory functions and lymphocytic infiltration into the lacrimal gland. To elucidate the early changes in the secretory pathway associ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.04.019
更新日期：2006-01-01 00:00:00
abstract：:The distribution of hyaluronan (HA) in the posterior eye wall from the vitreous through the sclera, with special consideration to localization in the retina and interphotoreceptor matrix (IPM), was evaluated in mouse tissues using an HA specific probe (bHABC, biotinylated hyaluronan binding complex). The vitreous body...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0369
更新日期：1997-11-01 00:00:00
abstract：:The purpose of this study was to determine whether endothelin B (ETB) receptor levels in the optic nerve are related to retinal ganglion cell (RGC) loss in a model of chronic endothelin-1 (ET-1) induced optic neuropathy. RGCs of adult Brown Norway rats were first retrogradely labeled with fluorochrome from the superio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.09.009
更新日期：2009-03-01 00:00:00
abstract：:Diabetic retinopathy is a complication of diabetes and a leading cause of vision loss among working-age adults. To assess whether the Wistar rat with Streptozotocin (STZ)-induced diabetes is a suitable animal model of human proliferative diabetic retinopathy we evaluated the vascular changes to assess the diabetic ret...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.04.025
更新日期：2019-07-01 00:00:00
abstract：:The function of acetylcholinesterase (AChE) is to terminate the action of acetylcholine at the cholinergic synapse. Recent evidence suggests additional roles for acetylcholinesterase as a peptidase and/or a protease which is expressed by growing neurites as part of their invasion of developing neural structures. We re...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(95)80001-8
更新日期：1995-02-01 00:00:00
abstract：:This review describes our recent attempts to develop new therapeutic modalities for corneal endothelial disease using animal models including non-human primate model in which the proliferative ability of corneal endothelial cells is severely limited, as is the case in humans. First, we describe our attempt to develop ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2011.10.014
更新日期：2012-02-01 00:00:00
abstract：:The objective of this study was to determine the cellular localization of P2Y(2) receptor gene expression in rabbit and primate ocular tissues using the technique of non-isotopic in situ hybridization. Fresh frozen whole eye from a New Zealand White rabbit and whole eye and eyelid from a rhesus macaque were cut into 5...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00068-x
更新日期：2003-07-01 00:00:00
abstract：:Antibiotics such as fluoroquinolones (FQLs) are commonly used to treat ocular infections but are also known to cause dermal melanocyte toxicity. The release of dispersed pigments from the iris into the aqueous humor has been considered a possible ocular side effect of the systemic administration of FQLs such as Moxifl...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.07.006
更新日期：2017-09-01 00:00:00
abstract：:Eleven monoclonal antibodies against different segments of bovine rhodopsin were used with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal photoreceptor cells for visualization by transmission electron microscopy. Antibodies against the C-terminus, F1-F2 loop and N-terminus of rho...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(86)90017-5
更新日期：1986-01-01 00:00:00
abstract：:Previous histochemical and biochemical studies have documented the presence of carbohydrate-containing molecules in the retinal interphotoreceptor matrix (IPM). The lectin peanut agglutinin (PNA), which preferentially binds galactose-containing carbohydrates, especially galactose-galactosamine linkages, selectively la...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80163-x
更新日期：1987-04-01 00:00:00
abstract：:Contact inhibition is an important mechanism for maintaining corneal endothelium in a non-replicative state. Protein tyrosine phosphatases (PTPs) play a role in regulating the integrity of cell-cell contacts, differentiation, and growth. In this study, we aimed to evaluate whether phosphatases are involved in the main...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.03.015
更新日期：2005-11-01 00:00:00
abstract：:Due to the high toxicity of aminoglycoside antibiotics, many authors prefer to use third generation cephalosporines in the prophylaxis and treatment of intraocular infections. The aim of the present study was to determine safe ceftazidime levels in anterior chamber irrigation solution. Twenty-two eyes of 12 white New ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0167
更新日期：1996-12-01 00:00:00
abstract：:The presence of the alpha(1)-adrenoceptor subtypes in various parts of the pig and rabbit eyes was investigated using [(3)H]-prazosin radioligand binding. The characterization of the subtypes was achieved by performing competition experiments with various subtype selective drugs. In the pig retina, both alpha(1A)- and...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0753
更新日期：2000-01-01 00:00:00
abstract：:The effect of dietary supplementation with acetylsalicylic acid on the increased modification, and susceptibility to modification, of lens crystallins from the streptozocin diabetic rat, has been determined. This was done by the measurement of characteristic markers of protein post-translational oxidative modification...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1186
更新日期：1993-12-01 00:00:00
abstract：:Autophagy is a lysosomal degradative process that is essential for cellular homeostasis and metabolic stress adaptation. Defective autophagy is involved in the pathogenesis of many diseases including granular corneal dystrophy type 2 (GCD2). GCD2 is an autosomal dominant disorder caused by substitution of histidine fo...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2015.09.008
更新日期：2016-03-01 00:00:00
abstract：:The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl-D-glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional gluc...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90011-8
更新日期：1983-02-01 00:00:00
abstract：:The evaluation of morphological changes of the acini in the meibomian glands is important for the diagnosis and management of dry eye related to meibomian gland dysfunction. While several tools have been developed to detect meibomian gland structure, infrared imaging is generally used in clinical settings. Unlike the ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.07.005
更新日期：2017-10-01 00:00:00
abstract：BACKGROUND:Indirect traumatic optic neuropathy (ITON) is a major cause of permanent loss of vision after blunt head trauma. Neuroinflammation plays a crucial role in neurodegenerative diseases. The present study concentrated on JNK/c-Jun-driven NLRP3 inflammasome activation in microglia during the degeneration of retin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108335
更新日期：2021-01-01 00:00:00
abstract：:Non-invasive imaging is an invaluable diagnostic tool in ophthalmology. Two imaging devices, the scanning laser ophthalmoscope (SLO) and spectral domain optical coherence tomography (SDOCT), emerged from the clinical realm to provide research scientists with a real-time view of ocular morphology in living animals. We ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.10.001
更新日期：2016-12-01 00:00:00
abstract：:This study demonstrates post-translational arginylation of bovine serum albumin and endogenous lens proteins by bovine lens arginyl-tRNA:protein transferase. This reaction has been proposed to be the first step in marking specific proteins for degradation by the non-lysosomal, ATP-dependent, ubiquitin-mediated proteol...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90220-9
更新日期：1991-11-01 00:00:00
abstract：:The purpose of this investigation was to explore the combined effects of single nucleotide polymorphisms (SNPs) within LFA-1/ICAM-1/GSK-3β pathway and environmental hazards on susceptibility to Graves' opthalmopathy (GO) among a Chinese Han population. Altogether 305 GO patients and 283 Graves' disease (GD) subjects w...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.05.022
更新日期：2018-09-01 00:00:00
abstract：:A duplex fluorescence assay to assess the viability of cells cultured in multi-well plates is described, which can be carried out in the original culture plate using a plate reader, without exchanges of culture or assay medium, or transfer of cells or cell supernatant. The method uses freshly prepared reagents and doe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.05.011
更新日期：2017-08-01 00:00:00
abstract：:Form-deprivation of chicks during early postnatal development results in ocular enlargement and great myopic refractive error (form-deprivation myopia). Previous studies have indicated that the retina, RPE and choroid play important roles in ocular enlargement in form-deprivation myopia. We aimed to isolate genes up-r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0765
更新日期：2000-02-01 00:00:00
