Abstract：
:The presence of the alpha(1)-adrenoceptor subtypes in various parts of the pig and rabbit eyes was investigated using [(3)H]-prazosin radioligand binding. The characterization of the subtypes was achieved by performing competition experiments with various subtype selective drugs. In the pig retina, both alpha(1A)- and alpha(1B)-adrenoceptors were detected and the proportion of sites was 70% alpha(1A)- and 30% alpha(1B)-adrenoceptors, respectively. In the pig iris, ciliary body and choroid, which are melanin-rich tissues, the non-specific binding of [(3)H]-prazosin was too high to detect any of the alpha(1)-adrenoceptor subtypes. However, in the albino rabbit iris, ciliary body and retina both alpha(1A)- and alpha(1B)-adrenoceptors were detected. The proportion of sites in the iris was 60 % alpha(1A)- and 40% alpha(1B)-adrenoceptors, respectively. In the ciliary body and rabbit retina the proportion of sites were 70% alpha(1A)- and 30% alpha(1B)-adrenoceptors. Only the alpha(1A)-adrenoceptor subtype was detected in the rabbit choroid.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Wikberg-Matsson A,Uhlén S,Wikberg JEdoi
10.1006/exer.1999.0753keywords:
subject
Has Abstractpub_date
2000-01-01 00:00:00pages
51-60issue
1eissn
0014-4835issn
1096-0007pii
S0014-4835(99)90753-4journal_volume
70pub_type
杂志文章abstract：:Photoreceptor outer segments (OS) in the vertebrate retina undergo a process of continual renewal involving shedding of disc membranes that are cleared by phagocytic uptake into the retinal pigment epithelium (RPE). In dystrophic Royal College of Surgeons (RCS) rats, OS phagocytosis is blocked by a mutation in the gen...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.08.006
更新日期：2015-11-01 00:00:00
abstract：:Albino mice exposed to 1 X 10(-4) W cm-2 of UV radiation in the range 290-400 nm for 12 hr per day developed anterior lens opacities within 1-2 months after onset of the exposure. Histological changes demonstrated marked derangement of the lens epithelium. When the radiation was filtered with glass, eliminating radiat...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(85)80011-7
更新日期：1985-10-01 00:00:00
abstract：:This review summarizes the Ernst H. Bárány Prize Lecture given at the meeting of the International Society of Eye Research in Geneva 2002. In the paper the path from the author's early studies on neurogenic inflammation in the eye to the search for a suitable prostaglandin analogue for glaucoma treatment, and the deve...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2003.11.016
更新日期：2004-04-01 00:00:00
abstract：:The shapes of two human lenses of different ages (27 and 46 years old) were altered by applying a stretching force to the ciliary muscle. The force was in the outward radial direction and was produced by an electronically driven stretching instrument which is described. The lenses were photographed for different degre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1168
更新日期：1993-11-01 00:00:00
abstract：:The spontaneously hypertensive rats (SHR) show a lower intraocular pressure--despite high arterial blood pressure--than the normotensive Wistar Kyoto rats (NR). In SHR the quantitative evaluation of resin casts and flat-mount preparations of the blood vessels of the ciliary body and choroid showed a significant dilati...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90004-1
更新日期：1985-02-01 00:00:00
abstract：:The irritative response to Nd:YAG laser capsulotomy was studied in unanaesthetized rabbits. Posterior lens capsulotomy with a total energy of 100 mJ had no effect on the pupil size but increased the intraocular pressure by 5-10 mmHg and caused a breakdown of the blood-aqueous barrier. Anterior lens capsulotomy with a ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(86)80030-6
更新日期：1986-10-01 00:00:00
abstract：:Retinitis Pigmentosa (RP) is an inherited disorder that may lead to blindness. In the rhodopsin S334ter-line-3 rat model of RP, the death of rods induces spatial rearrangement of cones into regular ring mosaics. Using this model, we discovered that the ring mosaics are restored to a homogeneous distribution upon appli...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.08.005
更新日期：2015-11-01 00:00:00
abstract：:Interleukin (IL)-1alpha and beta are important modulators of many functions of corneal epithelial and stromal cells that occur following injury to the cornea, including the influx of bone marrow-derived inflammatory cells into the stroma attracted by chemokines released from the stroma and epithelium. In this study, w...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.02.001
更新日期：2008-05-01 00:00:00
abstract：:SPACRCAN is a novel proteoglycan present in the interphotoreceptor matrix (IPM) of the rat and human retina that resists aqueous extraction through its binding to hyaluronan. The purpose of this study was: to clone mouse Spacrcan; to characterize the promoter elements; to define the deduced amino acid sequence; to est...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00273-7
更新日期：2003-01-01 00:00:00
abstract：:Although a range of changes in anterior segment structures have been documented to occur during accommodation, the quantification of changes in the structure of the anterior sclera during the accommodation process in human subjects has yet to be examined. This study therefore aimed to investigate the presence of short...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.07.023
更新日期：2018-12-01 00:00:00
abstract：:Mongolian gerbils were enucleated, and a crystal of HRP placed on the cut surface of the transected optic nerves emerging from the eyeballs. After incubation in an oxygenated medium, glutaraldehyde fixation, cryo-sectioning and reaction of the sections for peroxidase activity, HRP-labelled fibres were observed in the ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80093-3
更新日期：1987-12-01 00:00:00
abstract：:Rhegmatogenous retinal detachment is by far the most common indication for retinal surgery and a major cause of severe vision loss. Increased levels of glutamate found in the vitreous of human patients and persistent remodeling, even after reattachment, suggest substantial neurochemical, functional and anatomical chan...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.02.009
更新日期：2012-04-01 00:00:00
abstract：:Previous histochemical and biochemical studies have documented the presence of carbohydrate-containing molecules in the retinal interphotoreceptor matrix (IPM). The lectin peanut agglutinin (PNA), which preferentially binds galactose-containing carbohydrates, especially galactose-galactosamine linkages, selectively la...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80163-x
更新日期：1987-04-01 00:00:00
abstract：:Recent studies of the distribution and relative concentration of caveolin-1 in fractions of bovine lens epithelial and fiber cells have led to the novel concept that caveolin-1 may largely exist as a peripheral membrane protein in some cells. Caveolin-1 is typically viewed as a scaffolding protein for caveolae in plas...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.05.011
更新日期：2007-10-01 00:00:00
abstract：:Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. As a consequence of the unc...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.11.004
更新日期：2012-01-01 00:00:00
abstract：:In response to injury, the adult mammalian retina shows signs of structural remodeling, possibly in an attempt to preserve or regain some of its functional neural connections. In order to study the mechanisms involved in injury-induced plasticity, we have studied changes in growth associated protein 43 (GAP-43) after ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.01.006
更新日期：2007-05-01 00:00:00
abstract：:Incorporated in the luminal glycocalyx of vascular endothelia (EC) are negatively charged microdomains (anionic sites). These sites are considered functionally important (a) in their interaction with circulating blood constituents, and (b) as a determinant of vascular permeability. The molecular composition of these E...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80134-4
更新日期：1995-10-01 00:00:00
abstract：:The pre-retinal vitreous space of the eye of albino rabbits was perfused in vivo via a dialysis probe in order to measure the concentration of endogenous amino acids. Studies of isotope concentrations in the vitreal effluent following intracarotid injections of 3HOH and [14C]mannitol demonstrated that entrance of 3HOH...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80008-8
更新日期：1987-02-01 00:00:00
abstract：:The purpose of our study was to establish a system for culturing normal human conjunctival epithelial (NHCE) cells under serum-free culture conditions without compromising their ability to differentiate into a mucous epithelium. To this end, small pieces of normal conjunctiva were biopsied from patients undergoing cat...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.04.005
更新日期：2007-08-01 00:00:00
abstract：:A lesion in cGMP metabolism has been hypothesized to cause retinal degeneration in rd mice. Available cloned cDNAs coding for proteins involved in the cGMP cascade have been used to compare the corresponding retinal RNAs in the degenerative (rd/rd) mouse at 8-11 days with those in the 8-11-day-old morphologically norm...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80058-1
更新日期：1987-10-01 00:00:00
abstract：:Recently we determined that the Transient Receptor Potential Vanilloid 4 ion channel (TRPV4) has a crucial signaling role in a pathway that regulates various aspects of lens epithelium function. Here, we report on a different TRPV channel, TRPV1, in porcine lens. The presence of TRPV1 in the lens was evident from RT-P...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.006
更新日期：2018-07-01 00:00:00
abstract：:The influence of autonomic neurotransmitters on the growth and melanogenesis of cultured uveal melanocytes was studied. Uveal melanocytes were cultured with medium supplemented with cAMP elevating agents and basic fibroblast growth factor (complete medium). The cells were plated into multiple well plates, and various ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0869
更新日期：2000-09-01 00:00:00
abstract：:We have determined the pattern of RNA labeling (uridine incorporation) in the normal retina of the domestic cat. One eye in each of eight cats was labeled by injecting [3H]uridine into the vitreous cavity. Two of the labeled eyes had the lens and vitreous removed 10 days before labeling. Three additional animals recei...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90066-4
更新日期：1990-08-01 00:00:00
abstract：:Rabbit immunoglobulin G (IgG) binds specifically to the human corneal epithelium. We investigated this phenomenon to identify the binding material in the tissue. Sections of human cornea were immunostained with negative-control grade rabbit IgG, F(ab')(2) (the antigen recognition fragment of IgG yielded by pepsin dige...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.01.006
更新日期：2004-06-01 00:00:00
abstract：:A substantial change in the glycoconjugates of the rat interphotoreceptor matrix (IPM) has recently been shown to occur between post-natal day (P) 14 and P16 in rat retinas using lectin histochemistry. This suggests that the sialic acid content on the terminus of N-glycoside linked carbohydrate chains of the IPM incre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1013
更新日期：1993-01-01 00:00:00
abstract：:Previous studies suggest that an alpha 2-related isoform of the catalytic subunit is predominant in the lens fiber cells. The alpha 1 isoform is predominant in the lens epithelium (Garner, Horwitz and Enomoto, 1992). Data are presented to show that strophanthidin-sensitive K+ transport is sustained by both of these le...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1068
更新日期：1994-06-01 00:00:00
abstract：:Corneal endothelial wound healing following scrape injury in the rabbit and cat is characterized by cell spreading and maintenance of a normal endothelial phenotype consisting of apically-localized, circumferential microfilament bands and cell border-associated ZO-1, a tight junction protein and marker for endothelial...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0211
更新日期：1997-02-01 00:00:00
abstract：:Previous studies have relied on various electrodes or probes to monitor preretinal oxygen tension in an effort to gain insight into retinal oxygenation. In order to corroborate and extend the results of such studies, we developed a relatively non-invasive method of determining preretinal oxygen tension using 19F nucle...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90099-e
更新日期：1992-07-01 00:00:00
abstract：:Non-invasive imaging is an invaluable diagnostic tool in ophthalmology. Two imaging devices, the scanning laser ophthalmoscope (SLO) and spectral domain optical coherence tomography (SDOCT), emerged from the clinical realm to provide research scientists with a real-time view of ocular morphology in living animals. We ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.10.001
更新日期：2016-12-01 00:00:00
abstract：:The evaluation of morphological changes of the acini in the meibomian glands is important for the diagnosis and management of dry eye related to meibomian gland dysfunction. While several tools have been developed to detect meibomian gland structure, infrared imaging is generally used in clinical settings. Unlike the ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2017.07.005
更新日期：2017-10-01 00:00:00
