Abstract：
:A substantial change in the glycoconjugates of the rat interphotoreceptor matrix (IPM) has recently been shown to occur between post-natal day (P) 14 and P16 in rat retinas using lectin histochemistry. This suggests that the sialic acid content on the terminus of N-glycoside linked carbohydrate chains of the IPM increases between P14 and P16. In the present study, to test this hypothesis, we examined the developmental change of distribution of beta-galactoside alpha 2,6-sialyltransferase mRNA in rat retina using in situ hybridization histochemistry. C-DNA of rat alpha 2,6-sialyltransferase was isolated by PCR and cloned into the polylinker site of Bluescript KS (+). Antisense and sense RNA probes were labelled with digoxigenin-UTP by in vitro transcription with T3 and T7 RNA polymerases, respectively. Paraffin sections of rat retinas between P12 and P42 were incubated with the antisense or sense RNA probes. Specific labelling with the antisense probe was observed strongly in the cytoplasm of all ganglion cells and weakly in partial cells of the inner nuclear layers throughout the examined postnatal days. A remarkable change was observed in the photoreceptor cells between P14 and P16. Hybridization signals of the outer nuclear layer was observed from P14, while those of the inner segments were detected on P16 and thereafter. The alpha 2,6-sialyltransferase newly expressed in the inner segments on P16 appears to sialylate the Gal beta 1, 4GlcNAc residue of N-glycosidically linked glycoconjugates of the IPM, resulting in the change of lectin staining profiles.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Uehara F,Ohba N,Nakashima Y,Yanagita T,Ozawa M,Muramatsu Tdoi
10.1006/exer.1993.1013subject
Has Abstractpub_date
1993-01-01 00:00:00pages
89-93issue
1eissn
0014-4835issn
1096-0007pii
S0014-4835(83)71013-4journal_volume
56pub_type
杂志文章abstract：:Anthocyanins (ACs) are phenolic compounds that are distributed widely in fruits and vegetables. Although consumption of these compounds has been shown to improve visual function, the distribution of ACs in ocular tissue has not been examined in detail. The aim of this study was therefore to evaluate the ocular distrib...
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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更新日期：2007-03-01 00:00:00
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journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
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