Abstract:
:Eleven monoclonal antibodies against different segments of bovine rhodopsin were used with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal photoreceptor cells for visualization by transmission electron microscopy. Antibodies against the C-terminus, F1-F2 loop and N-terminus of rhodopsin were all observed to label bovine rod outer segments (ROS) densely, but to label rod inner segments (RIS) only sparsely. Most antibodies bound 200-600 gold particles per micron2 in the ROS, 10-60 gold particles per micron2 in the RIS and 5-20 particles per micron2 on the Lowicryl resin. One antibody against the N-terminus and one antibody against the C-terminus resulted in the binding of over 1000 particles per micron2 in bovine ROS. Cone outer segments (COS) were labeled with only one antibody, rho 3D6, having a specificity for the 1'-4' C-terminus of bovine rhodopsin. Ninety per cent of the COS were observed to be labeled with this antibody. Immunogold-dextran labeling was also used to study the cross-reactivity of these antibodies to rhodopsin in red and green frog ROS and COS. Monoclonal antibodies directed against sites along the F1-F2 loop, and the N-terminus labeled red frog ROS densely, but did not label either green ROS or COS. Three C-terminal specific antibodies against binding sites along the 1'-8' segment labeled both green and red ROS, but a higher extent of labeling was observed on the green ROS. Antibody rho 3D6, which bound to bovine COS, also labeled frog COS. These results indicate that the F1-F2 loop and segments along to the N-terminus and the C-terminus of bovine rhodopsin show a high degree of homology with corresponding regions of frog rhodopsin from red ROS; the C-terminal 1'-8' segment of bovine rhodopsin is closely related to the corresponding segment of frog rhodopsin from green ROS; and the 1'-4' C-terminus of bovine rhodopsin is highly homologous to segments of opsin from most bovine and frog COS. The labeling of frog red ROS in relation to multiple forms of rhodopsin observed by SDS-gel electrophoresis is discussed.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Hicks D,Molday RSdoi
10.1016/0014-4835(86)90017-5subject
Has Abstractpub_date
1986-01-01 00:00:00pages
55-71issue
1eissn
0014-4835issn
1096-0007pii
0014-4835(86)90017-5journal_volume
42pub_type
杂志文章abstract::The effect of a 'soft' anti-inflammatory corticosteroid on the corneal wound healing was investigated in rabbits. Albino rabbits were treated with soft steroid and dexamethasone after linear perforating corneal incision. Soft drug design is aimed to control and direct the drug activity and in vivo metabolism to nontox...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90229-n
更新日期:1990-02-01 00:00:00
abstract::A lesion in cGMP metabolism has been hypothesized to cause retinal degeneration in rd mice. Available cloned cDNAs coding for proteins involved in the cGMP cascade have been used to compare the corresponding retinal RNAs in the degenerative (rd/rd) mouse at 8-11 days with those in the 8-11-day-old morphologically norm...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80058-1
更新日期:1987-10-01 00:00:00
abstract:BACKGROUND:Absence of collagen VII causes blistering of the skin, eyes and many other tissues. This disease is termed dystrophic epidermolysis bullosa (DEB). Corneal fibrosis occurs in up to 41% and vision loss in up to 64% of patients. Standard treatments are supportive and there is no cure. The hypomorphic mouse mode...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.06.008
更新日期:2018-10-01 00:00:00
abstract::The suggested common regulator of the eye lens crystallin genes is c-Maf. Maf responsive elements have been detected in a number of crystallin promoters including that of the rat betaB2-crystallin gene. The betaB2-crystallin gene is active in the post-natal lens and its mRNA reaches its maximal level in the rat lens 6...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1077
更新日期:2001-11-01 00:00:00
abstract::The rapid increase in popularity of extracapsular cataract extraction may predispose the eye to postoperative bacterial infections by introducing viable organisms through the additional instrumentation and irrigation necessary for the extracapsular technique. Lens protein released into the aqueous humor of the anterio...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(89)80034-x
更新日期:1989-11-01 00:00:00
abstract::The purpose of the study was to determine histologic findings after successful laser trabeculoplasty in eyes with glaucoma. Previous studies have examined eyes in which laser did not lower intraocular pressure, or examined only the region of the laser burn itself. Nineteen autopsy eyes from 14 donors with either prima...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.07.009
更新日期:2007-10-01 00:00:00
abstract::Some previous studies have showed that transcorneal electrical stimulation (TES) could protect retinal neurons in certain rodent models. However, it is not yet clear whether TES could also definitely protect retinal neurons against ischemic insults. In the present study, we hypothesized that TES had such a neuroprotec...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.09.022
更新日期:2011-11-01 00:00:00
abstract::Infection of the corneal endothelial cells by human cytomegalovirus (CMV) is an important cause of corneal endotheliitis. CMV endotheliitis is difficult to completely cure and relapses are frequent. This can cause blinding corneal bullous keratopathy. However, the pathogenesis of CMV endotheliitis remains undetermined...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.06.017
更新日期:2017-08-01 00:00:00
abstract::Basement membranes isolated from the entire neural retinae of normal cows consist of a heterogeneous mixture of polypeptides resolvable as two major populations, one collagenous and the other non-collagenous. The non-collagenous population consists of disulfide-bonded aggregates of which the major fraction is soluble ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(83)90010-6
更新日期:1983-02-01 00:00:00
abstract::Tears serve as a viable diagnostic fluid with advantages including less invasive sample to collect and less complex to prepare for analysis. Several water-soluble and fat-soluble vitamins were detected and quantified in human tears and compared with blood serum levels. Samples from 15 family pairs, each pair consistin...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.12.007
更新日期:2017-02-01 00:00:00
abstract::Glaucoma is a leading cause of irreversible blindness worldwide. Lowering intraocular pressure (IOP) is the only strategy documented to delay the appearance and retard the progression of vision loss. One major approach for lowering IOP is to slow the rate of aqueous humor formation by the ciliary epithelium. As discus...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2008.11.005
更新日期:2009-04-01 00:00:00
abstract::The transport kinetics across the plasma-ocular barriers of labeled molecules including urea, D-glucose, 3-0-methyl-D-glucose, L-glucose, and sucrose were studied in adult RCS rats and compared to control albino Sprague-Dawleys (SD). Results indicate that substances that passively cross the plasma-aqueous and plasma-v...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(83)90156-2
更新日期:1983-09-01 00:00:00
abstract::Proliferative vitreoretinopathy (PVR) is a potentially blinding fibrotic complication. It is caused by the formation and contraction of epiretinal membranes (ERMs) that ultimately lead to retinal folds and traction retinal detachments. While multiple cell types have been identified in ERMs, retinal pigment epithelial ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.02.008
更新日期:2016-01-01 00:00:00
abstract::Crystallin-bound non-tryptophan fluorescence and protein cross-linking were studied in chronic galactosemia. Fluorescence (excitation/emission--370/440 nm) was significantly higher in galactosemic rats compared to controls (P less than 0.001). Accumulation of fluorescence was significantly reduced both in water solubl...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90153-l
更新日期:1990-10-01 00:00:00
abstract::There is increasing evidence implicating Transforming growth factor beta (TGF-beta) in pathological states of the lens. However, the underlying signalling mechanisms in human cells have not been fully examined. We have therefore investigated in a human lens cell line, FHL 124, the signalling characteristics of TGF-bet...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2003.08.006
更新日期:2004-03-01 00:00:00
abstract::The supply of L-carnosine, a bioactive dipeptide of β-alanine and l-histidine, to the retina across the blood-retinal barrier (BRB) was studied. The in vivo and in vitro studies revealed low uptake activities for [(3)H]Gly-Sar, a representative dipeptide, suggesting that l-carnosine transport plays only a minor role a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.06.002
更新日期:2013-08-01 00:00:00
abstract::The expression and intracellular localization of the estrogen receptor (ER) subtypes, ERalpha and ERbeta were examined in cultured human lens epithelial cells (HLE-B3). ERalpha and ERbeta mRNA expression was evaluated by coupled reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis. Subce...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2003.09.027
更新日期:2004-04-01 00:00:00
abstract::Herpes simplex virus type-1 (HSV-1) infection leads to impaired corneal sensation and, in severe cases, to corneal ulceration, melting and perforation. Here, we explore the potential therapeutic action of pigment epithelial-derived factor (PEDF) plus docosahexaenoic acid (DHA) on corneal inflammation and nerve regener...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.06.015
更新日期:2017-08-01 00:00:00
abstract::The phagocytosis of photoreceptor outer segments (OS) by the retinal pigment epithelium (RPE) is a receptor mediated process. A key component of this process is the receptor tyrosine kinase, Mer. RPE cells from the RCS rat, which lacks a functional mer gene, and do not express Mer protein, are able to bind OS, but are...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(03)00158-1
更新日期:2003-09-01 00:00:00
abstract::The distribution of hyaluronan (HA) in the posterior eye wall from the vitreous through the sclera, with special consideration to localization in the retina and interphotoreceptor matrix (IPM), was evaluated in mouse tissues using an HA specific probe (bHABC, biotinylated hyaluronan binding complex). The vitreous body...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0369
更新日期:1997-11-01 00:00:00
abstract::Angiotensin II and aldosterone are the main effectors of the renin-angiotensin aldosterone system (RAAS) and have a central role in hypertension as well as cardiovascular and renal disease. The localization of RAAS components within the retina has led to studies investigating the roles of angiotensin II, aldosterone a...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2019.107766
更新日期:2019-10-01 00:00:00
abstract::Long living animal models of retinitis pigmentosa (RP) can provide important information on the retinal changes that occur at the late stages of photoreceptor degeneration. The rhodopsin Pro347Leu transgenic rabbit (P347L Tg) is a model of RP, and it has been used to analyze the functional and morphological changes in...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.04.026
更新日期:2019-07-01 00:00:00
abstract::Paired human lenses ranging from 2 to 96 yr old (n = 17) were harvested within 2 hr post-mortem and immersed in liquid nitrogen. Phosphorus containing metabolites from perchloric acid extracts were quantitated using phosphorus-31 nuclear magnetic resonance spectroscopy. Low-energy metabolites detected include the phos...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(91)90016-8
更新日期:1991-06-01 00:00:00
abstract::Human limbal epithelial cells (LECs) intended for treatment of limbal stem cell deficiency are commonly cultivated on a 3T3 feeder layer with complex culture medium supplemented with fetal bovine serum (FBS). However, FBS is a xenogeneic component containing poorly characterised constituents and exhibits quantitative ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.06.019
更新日期:2018-11-01 00:00:00
abstract::Although primary cultures of human lens epithelial (HLE) cells provide important information concerning the role of epithelium in normal lens and cataract formation, the lack of a cell line precludes a broad range of studies on the metabolism and molecular biology of these cells. We have, therefore developed an HLE ce...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0551
更新日期:1998-11-01 00:00:00
abstract::Limbal microvascular endothelial cells (L-MVEC) contribute to formation of the corneal-limbal stem cell niche and to neovascularization of diseased and injuries corneas. Nevertheless, despite these important roles in corneal health and disease, few attempts have been made to isolate L-MVEC with the view to studying th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.12.008
更新日期:2015-02-01 00:00:00
abstract::In addition to its role as an inhibitor of urokinase plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1) is hypothesized to regulate epithelial cell adhesion and migration. We have previously reported that PAI-1 may be an important regulatory factor of the uPA system in cornea. The purpose of this s...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.06.006
更新日期:2005-01-01 00:00:00
abstract::Mice are now routinely utilized in studies of aqueous humor outflow dynamics. In particular, conventional aqueous outflow facility (C) is routinely measured via perfusion of the aqueous chamber by a number of laboratories. However, in mouse eyes perfused ex-vivo, values for C are variable depending upon whether the pe...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.08.011
更新日期:2017-11-01 00:00:00
abstract::The human lens crystallin gene CRYGC T5P is associated with Coppock-like cataract and has a phenotype of a dust-like opacity of the fetal lens nucleus and deep cortical region. Previous in vitro mutation studies indicate that the protein has changed conformation, solubility, and stability, which may make it susceptibl...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.08.021
更新日期:2008-12-01 00:00:00
abstract::Maintenance of cellular calcium levels is critical to cell function. Loss of calcium homeostasis might be a contributing factor to the development of cataract in the lens. In lens epithelium, calcium is involved in cell signaling and its precise regulation is vital. In this study, we investigated the regulation of sar...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2002.2049
更新日期:2002-11-01 00:00:00