Abstract：
:Rhegmatogenous retinal detachment is by far the most common indication for retinal surgery and a major cause of severe vision loss. Increased levels of glutamate found in the vitreous of human patients and persistent remodeling, even after reattachment, suggest substantial neurochemical, functional and anatomical changes have occurred in the detached retina. Therefore, this study was designed to characterize the morphological changes and glutamate receptor functionality in human rhegmatogenous retinal detachment. A cation channel permeating probe, agmatine (1-amino-4-guanidobutane; AGB), was employed to track endogenous and kainate (KA) driven channel functionality combined with immunocytochemical characterization of cellular remodeling. In the detached retina increased AGB permeability was identified in the outer retina while there was a decrease in the inner retina in basal conditions. KA receptors exhibited increased AGB permeability in ON bipolar cells and decreased permeability in calbindin labeled inner retinal cells. All retinal detachment samples demonstrated ectopic synaptic protein expression, photoreceptor processes extending toward the inner retina, and other remodeling features of retinal degeneration. These anatomical changes have been demonstrated in animal studies and are novel features unreported in primary cases of human retinal detachment. We conclude that deafferentation in retinal detachment leads to alteration of the glutamatergic pathway.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
de Souza CF,Kalloniatis M,Polkinghorne PJ,McGhee CN,Acosta MLdoi
10.1016/j.exer.2012.02.009subject
Has Abstractpub_date
2012-04-01 00:00:00pages
73-89issue
1eissn
0014-4835issn
1096-0007pii
S0014-4835(12)00077-2journal_volume
97pub_type
杂志文章abstract：:Cataract is a multifactorial disease, and a large variety of stressors induce cataracts. Many cataractogenic stressors and endoplasmic reticulum (ER) stressors induce the unfolded protein response (UPR) in various cell types. The UPR is known to produce reactive oxygen species (ROS) prior to the inducement of apoptosi...
journal_title：Experimental eye research
pub_type： 杂志文章
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abstract：:Elevated permeability of retinal endothelial cells (REC), as observed in diabetic retinopathy (DR), is induced by extended exposure to ≥25 ng/ml vascular endothelial growth factor A165 (VEGF165) for up to 3 d and this effect is more pronounced when equimolar amounts of basic fibroblast growth factor (bFGF) and insulin...
journal_title：Experimental eye research
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abstract：:[3H]-retinol binding to cellular retinol-binding protein (CRBP) increased more than five-fold in developing chicken corneal epithelium at 14 days of incubation, the time when the initial increase in corneal transparency occurs in the developing embryo. In contrast, [3H]-retinoic acid binding to cellular retinoic acid-...
journal_title：Experimental eye research
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doi：10.1016/0014-4835(83)90026-x
更新日期：1983-11-01 00:00:00
abstract：:The routine cultivation of human corneal endothelial cells, with the view to treating patients with endothelial dysfunction, remains a challenging task. While progress in this field has been buoyed by the proposed existence of progenitor cells for the corneal endothelium at the corneal limbus, strategies for exploitin...
journal_title：Experimental eye research
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doi：10.1016/j.exer.2014.07.002
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abstract：:Identification of novel agents to eradicate the residual lens cell population following cataract surgery provides one mode of preventing PCO formation. The present study investigated the biological mechanism of As(2)O(3) cytotoxicity in a human lens cell line and capsular bag system. FHL 124 cell survival was assessed...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.08.018
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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doi：10.1016/j.exer.2017.10.011
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abstract：:The development and growth of the vertebrate ocular lens is dependent on the regulated proliferation of an anterior monolayer of epithelial cells, and their subsequent differentiation into elongate fiber cells. The growth factor rich ocular media that bathes the lens mediates these cellular processes, and their respec...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.02.012
更新日期：2018-05-01 00:00:00
abstract：:Two-dimensional 1H NMR spectroscopic studies are presented on bovine gamma S- and gamma B-crystallin. In gamma S-crystallin, the four N-terminal residues have great flexibility compared with the rest of the molecule and assume a random coil conformation. NMR spectroscopy and electrospray mass spectrometry show that th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1099
更新日期：1994-08-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.05.004
更新日期：2013-09-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90136-8
更新日期：1985-05-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1016/j.exer.2005.08.008
更新日期：2006-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0463
更新日期：1998-05-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.05.002
更新日期：2009-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90054-v
更新日期：1992-03-01 00:00:00
abstract：:Hypoxic preconditioning protects photoreceptors against light-induced degeneration preserving retinal morphology and function. Although hypoxia inducible transcription factors 1 and 2 (HIF1, HIF2) are the main regulators of the hypoxic response, photoreceptor protection does not depend on HIF1 in rods. Here we used ro...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.12.008
更新日期：2016-05-01 00:00:00
abstract：:A substantial change in the glycoconjugates of the rat interphotoreceptor matrix (IPM) has recently been shown to occur between post-natal day (P) 14 and P16 in rat retinas using lectin histochemistry. This suggests that the sialic acid content on the terminus of N-glycoside linked carbohydrate chains of the IPM incre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1013
更新日期：1993-01-01 00:00:00
abstract：:Twenty-three human lenses from age 3-76 years were studied. Lenses obtained 24-36 post-mortem were exposed to monochromatic light (435.8 and 546.1 nm) in the I parallel and I+ modes and to white (tungsten lamp) unpolarized radiation. The light scattering intensities were obtained as a function of scattering angles fro...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90089-2
更新日期：1985-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.09.015
更新日期：2014-09-01 00:00:00
abstract：:The gene for microphthalmia in the homozygous cinnamon mouse produces colobomatous microphthalmia due to failure of closure of the optic fissure. Optic fissure formation and closure were examined in control cinnamon and homozygous microphthalmic foetuses from the 10th to the 13th gestational day using light and electr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90093-6
更新日期：1989-08-01 00:00:00
abstract：:To assess the ability of dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) to detect blood retinal barrier (BRB) damage in patients with diabetic macular edema (DME). DCE-MRI with 0.1 mmol Gd-DTPA was used to measure BRB permeability in 10 healthy and visually normal subjects and eight patients with DME, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.01.015
更新日期：2005-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.10.008
更新日期：2014-11-01 00:00:00
abstract：:Anatomic and physiological barriers limit drug delivery to the posterior segment of the eye via topical or systemic administration. Intravitreal administration has proven to be a safe and effective means of treating various posterior segment diseases. Elimination of a compound from the vitreous chamber may depend on l...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00311-1
更新日期：2003-03-01 00:00:00
abstract：:Ocular rigidity (OR) is thought to play a role in the pathogenesis of glaucoma, but the lack of reliable non-invasive measurements has been a major technical challenge. We recently developed a clinical method using optical coherence tomography time-lapse imaging and automated choroidal segmentation to measure the puls...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107831
更新日期：2020-01-01 00:00:00
abstract：:Phosducin is a retinal and pineal phosphoprotein assumed to play an important role in visual phototransduction. Phosducin is also a uveitopathogenic retinal antigen, but its potency has been reported to be mild. During the course of studies aimed at identifying uveitopathogenic sites in phosducin, we found that rat ph...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0379
更新日期：1997-11-01 00:00:00
abstract：:The mechanical, histological and pharmacological properties of the isolated cat ophthalmociliary artery are reported and compared for passively stretched ring segments from three locations on the artery. Ring segments were mounted in a modified myograph system after the method of Hogestatt, Andersson and Edvinsson (19...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90047-v
更新日期：1992-03-01 00:00:00
abstract：:There is increasing evidence that the macular pigment carotenoids, lutein and zeaxanthin, may play an important role in the prevention of age-related macular degeneration, cataract, and other blinding disorders. Although it is well known that the retina and lens are enriched in these carotenoids, relatively little is ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0954
更新日期：2001-03-01 00:00:00
abstract：:The histopathological features of uveitis and retinal vasculitis in acute experimental allergic encephalomyelitis (EAE) were investigated using light and electron microscopy. Lewis rats were immunized by spinal cord homogenate, complete Freund's adjuvant and Bordetella pertussis. The eyes of rats with EAE exhibited va...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1024
更新日期：1993-02-01 00:00:00
abstract：:To investigate the nature of the 140 kDa glycoprotein in the trabecular meshwork, polypeptides were extracted with either urea/sodium dodecyl sulfate (SDS)/beta-2-mercaptoethanol (BME) or guanidine hydrochloride followed by pepsin digestion. After electrophoresis and immunoblotting with anti-type-VI-collagen antibodie...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1006
更新日期：1994-02-01 00:00:00
abstract：:An enzymatic procedure for the estimation of organic hydroperoxides has been adapted to biological tissues and applied to the measurement of hydroperoxides in the rat retina. Hydroperoxides are determined from the coupled activities of glutathione peroxidase and glutathione reductase as measured by the loss of NADPH a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90116-1
更新日期：1983-03-01 00:00:00
