Abstract:
:Previous studies suggest that an alpha 2-related isoform of the catalytic subunit is predominant in the lens fiber cells. The alpha 1 isoform is predominant in the lens epithelium (Garner, Horwitz and Enomoto, 1992). Data are presented to show that strophanthidin-sensitive K+ transport is sustained by both of these lens Na,K-ATPases. The K50 for strophanthidin inhibition of K+ transport was 1.4 +/- 0.5 x 10(-6) M for the alpha 1 isoform of the epithelium, 1.3 +/- 0.6 x 10(-7) M for the alpha 2-related isoform of the lens fiber cells. Na+ accumulation in cultured bovine lenses was strophanthidin sensitive. The K50 values for strophanthidin-sensitive Na+ accumulation were similar to those obtained for K+ transport. ARP turnover by the lens fiber cell Na,K-ATPase (1700 +/- 600 min-1) was lower than ATP turnover by the lens epithelium Na,K-ATPase (8000 +/0 1000 min-1). Both lens Na,K-ATPases form the (ouabain + Mg(2+) + phosphate)-dependent phosphoenzyme. Both lens Na,K-ATPases form the (ATP + Na(+) + Mg2+)-dependent phosphorylated intermediate. K+ does not effectively dephosphorylate the Na,K-ATPase of the lens fibers. K+ does cause dephosphorylation of the Na,K-ATPase of the lens epithelium. Interaction of the Na,K-ATPase with Mg2+ would appear to cause the monovalent cation insensitivity. The lens fiber cell Na,K-ATPase, like the lens epithelium Na,K-ATPase occludes two K+ ions. However, between the two major Na,K-ATPases of the lens, there would appear to be differences in the ATP dissolution of the K-occluded state.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Garner MHdoi
10.1006/exer.1994.1068subject
Has Abstractpub_date
1994-06-01 00:00:00pages
705-18issue
6eissn
0014-4835issn
1096-0007pii
S0014-4835(84)71068-2journal_volume
58pub_type
杂志文章abstract::This study was designed to determine the safety and efficacy of transferrin-ricin A chain toxin (Tfr-rRA) at preventing retinal detachment in a rabbit model of proliferative vitreoretinopathy (PVR). The toxicity of intravitreal Tfr-rRA (1000-5000 ng) was determined by indirect ophthalmoscopy and electroretinography on...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0079
更新日期:1996-06-01 00:00:00
abstract::The retinoschisin protein is encoded on the short arm of the X-chromosome by RS1, is expressed abundantly in photoreceptor inner segments and in bipolar cells, and is secreted as an octamer that maintains the structural integrity of the retina. Mutations in RS1 lead to X-linked retinoschisis (XLRS), a disease characte...
journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2004.08.024
更新日期:2005-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90096-4
更新日期:1984-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(89)80079-x
更新日期:1989-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0579
更新日期:1999-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.12.004
更新日期:2014-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1107
更新日期:1994-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.05.013
更新日期:2013-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2009-06-15 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.04.001
更新日期:2018-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(88)80061-7
更新日期:1988-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90031-4
更新日期:1985-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1999.0733
更新日期:1999-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0116
更新日期:1996-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.06.015
更新日期:2017-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.12.011
更新日期:2012-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2006.02.011
更新日期:2006-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.05.017
更新日期:2010-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.05.011
更新日期:2004-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90004-1
更新日期:1985-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0600
更新日期:1999-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.01.025
更新日期:2018-04-01 00:00:00
abstract::In normal retina the developing photoreceptor cells first show presence of opsin over the distal ends of the ciliary protrusions. In a fully differentiated cell intense activity is seen over the rod outer-segment discs; some activity is also seen over the Golgi zone and near the distal ends of the inner segments but t...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80170-7
更新日期:1987-03-01 00:00:00