Abstract:
:The influence of autonomic neurotransmitters on the growth and melanogenesis of cultured uveal melanocytes was studied. Uveal melanocytes were cultured with medium supplemented with cAMP elevating agents and basic fibroblast growth factor (complete medium). The cells were plated into multiple well plates, and various concentrations of adrenergic and cholinergic agents were added to the media (complete medium or various deleted media). After 6 days, the cells were detached for cell counting and melanin measurement and compared to controls. Epinephrine, isoproterenol, salbutamol and metaproterenol (adrenergic agonists that can activate beta(2)-adrenoceptors) substantially stimulated growth and melanogenesis of cultured uveal melanocytes in cAMP-deleted medium. Methoxamine, clonidine, prenalterol and D-7114 (adrenergic agonists that do not activate beta(2)-adrenoceptors) showed no effect under similar experimental conditions. Muscarine (a cholinergic agonist) inhibited the growth and melanogenesis of uveal melanocytes in complete medium. It indicates that adrenergic agents (beta(2)-adrenoceptor agonists) stimulate growth and melanogenesis in uveal melanocytes, while cholinergic agonist has an inhibitory effect. This effect appears to involve the cAMP second messenger system. These studies suggest that homeostasis of the uveal melanocytes may be maintained, in part, by regulating the autonomic nervous system in vivo.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Hu DN,Woodward DF,McCormick SAdoi
10.1006/exer.2000.0869keywords:
subject
Has Abstractpub_date
2000-09-01 00:00:00pages
217-24issue
3eissn
0014-4835issn
1096-0007pii
S0014-4835(00)90869-8journal_volume
71pub_type
杂志文章abstract::The photopigment of avian pineal which mediates light sensitivity was sought via its chromophore. Chick pineal cells in primary cultures were incubated overnight in the dark with [3H]retinol. Reduction of Schiff's bases with cyanoborohydride prior to SDS-PAGE revealed radioactivity bound to a 30 kDa component in pinea...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(88)80042-3
更新日期:1988-06-01 00:00:00
abstract::The localization of [35S]-sulfate and [3H]-glucosamine in the hamster non-pigmented ciliary epithelium was studied by light and electron microscopic autoradiography. The radioactivity was concentrated in the Golgi complex of the non-pigmented ciliary epithelium 10 mins after injections of [35S]-sulfate or [3H]-glucosa...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(83)90009-x
更新日期:1983-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1156
更新日期:1993-11-01 00:00:00
abstract::The purpose of this work was to investigate the effect of c-fos antisense oligonucleotide (c-fos-AS-ON) on proliferative vitreoretinopathy (PVR). Cultures of human retinal pigment epithelial (hRPE) cells were established from adult human corneal donors. These cells were positively stained for cytokeratins. C-fos-AS-ON...
journal_title:Experimental eye research
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doi:10.1016/j.exer.2006.07.020
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2014-06-01 00:00:00
abstract::Specific [3H]-dexamethasone binding sites were demonstrated in bovine corneal cytosol using titration analyses. Specific glucocorticoid binding to corneal cytosol exhibited a single class of sites with apparent dissociation constants of 4-6 nM and 68-72 fmol binding sites mg-1 cytosolic protein. Association of steroid...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90188-x
更新日期:1984-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.05.031
更新日期:2018-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 信件
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更新日期:2009-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(95)80001-8
更新日期:1995-02-01 00:00:00
abstract::Platelet-rich fibrin (PRF) is a natural biomaterial and has excellent biochemical and physical properties with a history of proven biocompatibility in the field of tissue engineering and regenerative medicine. Recent reports of fibrin-based matrices have offered new opportunities to apply PRF as a supplement for in vi...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.04.013
更新日期:2018-08-01 00:00:00
abstract::Recently we determined that the Transient Receptor Potential Vanilloid 4 ion channel (TRPV4) has a crucial signaling role in a pathway that regulates various aspects of lens epithelium function. Here, we report on a different TRPV channel, TRPV1, in porcine lens. The presence of TRPV1 in the lens was evident from RT-P...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.04.006
更新日期:2018-07-01 00:00:00
abstract::Arf encodes an important tumor suppressor, p19(Arf), which also plays a critical role to control hyperplasia in the primary vitreous during mouse eye development. In the absence of Arf, mice are born blind and display a phenotype closely mimicking severe forms of the human eye disease, persistent hyperplastic primary ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.01.004
更新日期:2016-04-01 00:00:00
abstract::Lens epithelial cells undergo a dramatic transformation during the process of differentiation into elongated fiber cells. The membrane-associated actin cytoskeleton is likely to play a critical role in the stabilization and maintenance of the highly elongated fiber cell shape. Tropomodulin is a tropomyosin-binding pro...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0111
更新日期:1996-08-01 00:00:00
abstract::Post-translational modifications and/or structural changes induced by modifications are likely causes of the decrease in crystallin solubility associated with aging and the development of cataract. Characterization of human lens crystallins by mass spectrometry has demonstrated that betaB2-crystallin undergoes less mo...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1023
更新日期:2001-08-01 00:00:00
abstract::Giant papillae, characteristic lesions of vernal keratoconjunctivitis, are formed as a result of the proliferation of conjunctival fibroblasts, the deposition of extracellular matrix, and the infiltration of inflammatory cells. The concentration of interleukin (IL)-4 is also increased in the tear fluid of individuals ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(02)00248-8
更新日期:2003-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0177
更新日期:1997-01-01 00:00:00
abstract::Electron-microscope immunocytochemistry and antibody staining of nitrocellulose replicas of SDS gels (Western blots) were used in a developmental study to detect the presence and localization of opsin in the developing photoreceptors of rds (020/A) mutant mice and their BALB/c normal controls. Western blot analysis of...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80061-1
更新日期:1987-10-01 00:00:00
abstract::In this study, we examined calcium-mediated degradation of a neurofilament protein (NFP), and autolytic activation of calpain in Lewis rat optic nerve in vitro. After incubation with calcium, homogenized optic nerve samples were analysed by SDS-PAGE in association with ECL immunoblot techniques. 68 kD NFP, calpain, an...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0286
更新日期:1997-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.05.005
更新日期:2006-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.05.017
更新日期:2004-10-01 00:00:00
abstract::The ability of diinosine polyphosphates, diinosine triphosphate (Ip(3)I), diinosine tetraphosphate (Ip(4)I) and diinosine pentaphosphate (Ip(5)I) to modify intraocular pressure in normotensive New Zealand white rabbits was tested. Ip(5)I produced increase in intraocular pressure, while Ip(3)I and Ip(4)I produced a dec...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.05.011
更新日期:2012-08-01 00:00:00
abstract::Glycoconjugate mucin secretion from conjunctival goblet cells is tightly regulated by nerves and specialized pro-resolving mediators (SPMs) to maintain ocular surface health. Here we investigated the actions of the SPM resolvin E1 (RvE1) on cultured rat conjunctival goblet cell glycoconjugate secretion and intracellul...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.04.015
更新日期:2018-08-01 00:00:00
abstract::Thrombospondin-1 (TSP-1) is a multifunctional matrix protein that has recently been examined in various wound processes, primarily for its ability to activate the latent complex of transforming growth factor-beta (TGF-β). TGF-β has been shown to play a major role in stimulating mesenchymal cells to synthesize extracel...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.06.018
更新日期:2011-10-01 00:00:00
abstract:PURPOSE:To assess the inter-ocular and inter-session reliability for a range of parameters derived from the photopic electroretinogram (ERG) in a group of normal non-human primates. METHODS:Inter-ocular differences for photopic ERGs were assessed in a group of normal anesthetized adult rhesus monkeys (Macaca mulatta, ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2003.09.013
更新日期:2004-01-01 00:00:00
abstract::Harmonic analysis was used to characterize the rabbit flicker ERG elicited by sinusoidally modulated full-field stimuli under light-adapted conditions. The frequency-response function for fundamental amplitude, derived from Fourier analysis of the ERG waveforms, exhibited two limbs, with an amplitude minimum at approx...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.10.005
更新日期:2010-12-01 00:00:00
abstract::The retinoschisin protein is encoded on the short arm of the X-chromosome by RS1, is expressed abundantly in photoreceptor inner segments and in bipolar cells, and is secreted as an octamer that maintains the structural integrity of the retina. Mutations in RS1 lead to X-linked retinoschisis (XLRS), a disease characte...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108344
更新日期:2021-01-01 00:00:00
abstract::Multidrug resistance associated protein (MRP) is a major family of efflux transporters involved in drug efflux leading to drug resistance. The objective of this study was to explore physical barriers for ocular drug absorption and to verify if the role of efflux transporters. MRP-2 is a major homologue of MRP family a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.08.015
更新日期:2007-01-01 00:00:00
abstract::Myocilin is a 55-57kDa secreted glycoprotein and member of the olfactomedin family, which is mutated in some forms of primary open-angle glaucoma. To assess the effects of elevated amounts of myocilin on aqueous humor outflow dynamics in an in vivo system, transgenic betaB1-crystallin-MYOC mice have been developed tha...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.06.006
更新日期:2008-09-01 00:00:00