Abstract:
:Arf encodes an important tumor suppressor, p19(Arf), which also plays a critical role to control hyperplasia in the primary vitreous during mouse eye development. In the absence of Arf, mice are born blind and display a phenotype closely mimicking severe forms of the human eye disease, persistent hyperplastic primary vitreous (PHPV). In this report, we characterize p19(Arf) expression in perivascular cells that normally populate the primary vitreous and express the Arf promoter. Using a new ex vivo model, we show that these cells respond to exogenous Tgfβ, despite being isolated at a time when Tgfβ has already turned on the Arf promoter. Treatment of the cells with PDGF-B ligand doubles the population of cells in S-phase and ectopic expression of Arf blunts that effect. We show this effect is mediated through Pdgfrβ as expression of Arf represses expression of Pdgfrβ mRNA and protein to approximately 60%. p53 is not required for Arf-dependent blockade of PDGF-B driven proliferation and repression of Pdgfrβ protein as ectopic expression of Arf is still able to inhibit the 2-fold increase in the S-phase fraction of cells upon treatment with PDGF-B. Finally, induction of mature miR-34a, a microRNA previously identified to be regulated by p19(Arf) does not depend on p53 while the expression of the primary transcript does require p53. These data corroborate that, as in vivo, p19(Arf) functions to inhibit PDGF-B driven proliferation ex vivo.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Iqbal NS,Devitt CC,Sung CY,Skapek SXdoi
10.1016/j.exer.2016.01.004subject
Has Abstractpub_date
2016-04-01 00:00:00pages
224-229eissn
0014-4835issn
1096-0007pii
S0014-4835(16)30004-5journal_volume
145pub_type
杂志文章abstract::Two-dimensional 1H NMR spectroscopic studies are presented on bovine gamma S- and gamma B-crystallin. In gamma S-crystallin, the four N-terminal residues have great flexibility compared with the rest of the molecule and assume a random coil conformation. NMR spectroscopy and electrospray mass spectrometry show that th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1099
更新日期:1994-08-01 00:00:00
abstract::Retinal neovascularization (NV) due to retinal ischemia is one of the major causes of vision reduction in patients with different types of retinal diseases although anti-vascular endothelial growth factor (anti-VEGF) therapy can partially reduce the size of the retinal NV. We recently reported that periostin plays an ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.10.012
更新日期:2016-12-01 00:00:00
abstract::Docosahexaenoic acid is the major polyunsaturated fatty acid in photoreceptor membrane phospholipids and is thought to be necessary for photoreceptor function. Docosahexaenoic acid may be metabolized to docosanoids or, by retroconversion, to eicosapentaenoic acid followed by lipoxygenation and synthesis of n-3 metabol...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0116
更新日期:1996-09-01 00:00:00
abstract::Superoxide radicals or products generated by these radicals in a xanthine/xanthine oxidase system lyse cultured rat and human corneal epithelial cells as measured in a chromium-51 release assay. Partial protection from this lysis is afforded by superoxide dismutase and complete protection is obtained with catalase. Hy...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90004-e
更新日期:1990-01-01 00:00:00
abstract::Müller cells can completely repair retinal injury by acting as endogenous stem/progenitor cells in lower-order vertebrates. However, a safe and effective approach to activate progenitor potential of retinal Müller cells in higher-order vertebrates, which rarely re-enter the cell cycle, is a bottleneck problem. In the ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.01.008
更新日期:2015-03-01 00:00:00
abstract::Photoreceptor outer segments (OS) in the vertebrate retina undergo a process of continual renewal involving shedding of disc membranes that are cleared by phagocytic uptake into the retinal pigment epithelium (RPE). In dystrophic Royal College of Surgeons (RCS) rats, OS phagocytosis is blocked by a mutation in the gen...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.08.006
更新日期:2015-11-01 00:00:00
abstract::Small heat shock proteins and alpha-crystallins are related proteins with several common structural and functional properties including homologous amino acid sequences and similar chaperone-like activity. Furthermore, small heat shock proteins and alpha-crystallins are phosphorylated in vivo at serine residues within ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0324
更新日期:1997-08-01 00:00:00
abstract::Homocysteine is an amino acid required for the metabolism of methionine. Excess homocysteine is implicated in cardiovascular and neurological disease and new data suggest a role in various retinopathies. Mice lacking cystathionine-beta-synthase (cbs(-/-)) have an excess of retinal homocysteine and develop anatomical a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.12.011
更新日期:2012-03-01 00:00:00
abstract::Purified retinal pigment epithelial cells of bovine eyes have been fractionated by a series of buffer and detergent extractions. The electropherogram of the buffer-insoluble, Triton X-100-soluble fraction (RPE-TS) exhibits a major polypeptide band of M(r) 65 kDa and a variety of minor components. Electrophoretically p...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90008-g
更新日期:1992-12-01 00:00:00
abstract::The retinal ganglion cell (RGC)-like RGC-5 line can be differentiated with staurosporine to stop dividing, extend neurites, and increase levels of several ganglion cell markers. This allows study of regulation of neurite development on a single cell basis. However, it is unclear whether the neurites induced by differe...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.08.001
更新日期:2007-11-01 00:00:00
abstract::Tenon's fibroblast (TF) bleb encapsulation is a common cause of filtering surgery failure. Various pharmacologic agents have been used in an attempt to inhibit this response. The effects of three angiogenesis inhibitors were studied. AGM-1470, a fumagillin analog and hydrocortisone 21-phosphate (HC21P) complexed with ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1037
更新日期:1994-04-01 00:00:00
abstract::Developing canine retina, fixed in Bouin's or Tellyesnizky's acetic alcoholic formalin, was examined with a battery of biotinylated lectins. ConA and WGA consistently bound to all retinal layers including the tapetum cellulosum, retinal pigment epithelium, inner and outer segments, plexiform layers and ganglion cells....
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90150-s
更新日期:1990-10-01 00:00:00
abstract::Iron is an essential biometal in the aqueous humor, the principal source of nutrients for the avascular cornea and the lens. Here, we explored whether the ciliary body (CB), the source of aqueous humor, transports iron, and if the prion protein (PrPC) facilitates this process as in the outer retina. Using a combinatio...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.05.031
更新日期:2018-10-01 00:00:00
abstract::Thrombospondin-1 (TSP-1) is a multifunctional matrix protein that has recently been examined in various wound processes, primarily for its ability to activate the latent complex of transforming growth factor-beta (TGF-β). TGF-β has been shown to play a major role in stimulating mesenchymal cells to synthesize extracel...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.06.018
更新日期:2011-10-01 00:00:00
abstract::Increasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal inflammatory changes in diabetes have been reported and in vivo choroidal thickness (CT) has been searched as a marker of retinopathy with contradictory results. We aimed to investigate the early stag...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108195
更新日期:2020-10-01 00:00:00
abstract::PAX6-related Aniridia is a sight-threatening disease involving progression of secondary glaucoma and aniridia related keratopathy (ARK). Change or loss of limbal epithelial progenitors causes epithelial surface defects. We analyzed the effect of PAX6 on mRNA expression changes with a two-step approach, as follows. Fir...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.10.002
更新日期:2019-02-01 00:00:00
abstract::The ability of diinosine polyphosphates, diinosine triphosphate (Ip(3)I), diinosine tetraphosphate (Ip(4)I) and diinosine pentaphosphate (Ip(5)I) to modify intraocular pressure in normotensive New Zealand white rabbits was tested. Ip(5)I produced increase in intraocular pressure, while Ip(3)I and Ip(4)I produced a dec...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.05.011
更新日期:2012-08-01 00:00:00
abstract::The genetically encoded green fluorescent protein-based calcium sensor, GCaMP, has been used to detect calcium transients and report neuronal activity. We evaluated the specificity of GCaMP3 expression to retinal ganglion cells (RGCs) of the transgenic Thy1-GCaMP3 mouse line in healthy control animals and in those aft...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108297
更新日期:2021-01-01 00:00:00
abstract::Laser Doppler flowmetry (LDF) measures the flux of red blood cells in a vascular network embedded in tissue. In the present report, choroidal and ciliary body blood flow was measured continuously using an LDF technique in pentobarbital anesthetized cats. Blood flow measurements were made from the choroid beneath the l...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(91)90068-p
更新日期:1991-08-01 00:00:00
abstract::(O-acyl) ω-hydroxy fatty acids (OAHFAs) are a recently found group of polar lipids in meibum. Since these lipids can potentially serve as a surfactant in the tear film lipid layer, the surface properties of a molecule of this lipid class was investigated and compared with a structurally related wax ester and a fatty a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.06.016
更新日期:2013-10-01 00:00:00
abstract::Nerve growth factor in the tear film and corneal epithelium is hypothesized to play an important role in ocular surface maintenance and corneal wound healing. The purpose of this study was to determine the expression of nerve growth factor and its high affinity (trkA) receptor in tears, cornea, and lacrimal glands of ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.11.013
更新日期:2005-05-01 00:00:00
abstract::Gene therapy has been proposed as a feasible strategy for RGC survival and optic nerve regeneration. Some preclinical and clinical studies revealed intraocular inflammation after intravitreal injection of adeno-associated virus (AAV) by slit-lamp or indirect ophthalmoscope. Here we evaluate the longitudinal profile of...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.107955
更新日期:2020-04-01 00:00:00
abstract::The binding of monoclonal and polyclonal antibodies to glial fibrillary acidic protein (GFAP) antigenic sites in the rabbit retina was shown to be sensitive to aldehyde fixation. In chemically unfixed retina, the polyclonal anti-GFAP labeled Müller cells, astrocytes, and unidentified profiles in the outer plexiform la...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90139-l
更新日期:1990-04-01 00:00:00
abstract::Binocular vision provides the most accurate and precise depth information; however, many people have impairments in binocular visual function. It is possible that other sensory inputs could be used to obtain reliable depth information when binocular vision is not available. However, it is currently unknown whether dep...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.08.013
更新日期:2019-06-01 00:00:00
abstract::Dexamethasone is a glucocorticoid that is widely used in the ophthalmic arena. The recent FDA approved dexamethasone implant can provide a three month efficacy but with high rate of drug related cataract and high intraocular pressure (IOP). It seems that higher steroid in aqueous humor and around lens may be associate...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.11.002
更新日期:2014-12-01 00:00:00
abstract:PURPOSE:This study examined the patterned treatment of corneal collagen cross-linking (CXL) for keratoconus to reduce the complications caused by ultraviolet (UV) irradiation. By modifying the method of UV irradiation during the cross-linking process, cross-linking with a special structure is achieved, and the cross-li...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2021.108450
更新日期:2021-01-23 00:00:00
abstract::Lens development and transparency have been hypothesized to depend on intercellular gap junction channels, consisting of alpha3 (Cx46) and alpha8 (Cx50) connexin subunits, to transport metabolites, secondary messages and ions between lens cells. To evaluate this hypothesis, we have generated alpha3(-/-) alpha8(-/-) do...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.03.013
更新日期:2006-09-01 00:00:00
abstract::Adipose-Derived Stem Cells (ADSCs) have an important contribution in regenerative medicine ranging from testing stem cell therapy for disease treatment in pre-clinical models to clinical trials. For immediate use of stem cells for therapy, there is a requirement of the high dose of stem cells at different time points ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107860
更新日期:2019-12-01 00:00:00
abstract::Myopia is the most common form of refractive eye disease, and the prevalence is increasing rapidly worldwide. However, the key metabolic alterations in individuals with high myopia are not understood clearly, and serum biomarkers remain to be determined. The objectives of this study were to identify serum biomarkers a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107737
更新日期:2019-09-01 00:00:00
abstract::Mice are now routinely utilized in studies of aqueous humor outflow dynamics. In particular, conventional aqueous outflow facility (C) is routinely measured via perfusion of the aqueous chamber by a number of laboratories. However, in mouse eyes perfused ex-vivo, values for C are variable depending upon whether the pe...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.08.011
更新日期:2017-11-01 00:00:00