GCaMP3 expressing cells in the ganglion cell layer of Thy1-GCaMP3 transgenic mice before and after optic nerve injury.

Abstract:

:The genetically encoded green fluorescent protein-based calcium sensor, GCaMP, has been used to detect calcium transients and report neuronal activity. We evaluated the specificity of GCaMP3 expression to retinal ganglion cells (RGCs) of the transgenic Thy1-GCaMP3 mouse line in healthy control animals and in those after optic nerve transection (ONT). Retinas from control mice (n = 4) were isolated and stained for RNA-binding protein with multiple splicing (RBPMS) and choline acetyltransferase (ChAT), specific markers for RGCs and cholinergic amacrine cells, respectively. GCaMP3 expression was enhanced with green fluorescent protein (GFP) immunoreactivity. In one subset of animals, ONT was performed 3, 7, or 14 days before sacrifice (n = 4, 4, 4, respectively). Cells positive for GCaMP3, RBPMS, ChAT, as well as the population of co-labeled cells, were quantified. In another subset of animals (n = 4), in vivo confocal scanning laser ophthalmoscope imaging was performed in the same mice at baseline and at 3, 7 and 14 days after ONT. The mean (SD) densities of GCaMP3, RBPMS, and ChAT expressing cells in control retinas were 2663 (110), 3401 (175), and 1041 (47) cells/mm2, respectively. Of the GCaMP3+ cells, 92 (1)% were co-labeled with RBPMS, while 72 (1)% of RBPMS-labeled cells expressed GCaMP3. ChAT expressing cells were not co-labeled with GCaMP3. The number of GCaMP3+ and RBPMS+ cells decreased dramatically after ONT; 78%, 39%, and 18% of GCaMP3+ and 80%, 40%, and 15% of RBPMS+ cells, relative to control retinas, survived at 3, 7, and 14 days after ONT. However, the number of ChAT+ cells did not change. There was a progressive decrease in GCaMP3 fluorescence after ONT in in vivo images. The majority of RGCs in the ganglion cell layer of Thy1-GCaMP3 mice express GCaMP3. There was an expected progressive and specific loss of GCaMP3 expression after ONT. Thy1-GCaMP3 transgenic mice have potential for longitudinal assessment of RGCs in injury models.

journal_name

Exp Eye Res

authors

Yabana T,Hooper ML,Farrell SR,Chauhan BC

doi

10.1016/j.exer.2020.108297

subject

Has Abstract

pub_date

2021-01-01 00:00:00

pages

108297

eissn

0014-4835

issn

1096-0007

pii

S0014-4835(20)30555-8

journal_volume

202

pub_type

杂志文章
  • Corneal endothelial cell matrix promotes expression of differentiated features of retinal pigmented epithelial cells: implication of laminin and basic fibroblast growth factor as active components.

    abstract::Human retinal pigmented epithelial (RPE) cells cultured on plastic, unlike RPE in situ, often fail to density arrest, do not produce melanin, and do not express mRNA for cellular retinaldehyde binding protein (CRALBP). When human RPE are cultured on the extracellular matrix produced by bovine corneal endothelial cells...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1006/exer.1993.1158

    authors: Campochiaro PA,Hackett SF

    更新日期:1993-11-01 00:00:00

  • Cytolysis of corneal epithelial cells by hydrogen peroxide.

    abstract::Superoxide radicals or products generated by these radicals in a xanthine/xanthine oxidase system lyse cultured rat and human corneal epithelial cells as measured in a chromium-51 release assay. Partial protection from this lysis is afforded by superoxide dismutase and complete protection is obtained with catalase. Hy...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/0014-4835(90)90004-e

    authors: Hayden BJ,Zhu L,Sens D,Tapert MJ,Crouch RK

    更新日期:1990-01-01 00:00:00

  • Clinical trials with aldose reductase inhibitors.

    abstract::Following the identification of potent, orally active inhibitors of aldose reductase, the next challenge comes in demonstrating clinical utility in the treatment of diabetic complications. It is not feasible to conduct pivotal studies in all indications within the same trial and neuropathy evolved as the lead indicati...

    journal_title:Experimental eye research

    pub_type: 临床试验,杂志文章,评审

    doi:10.1016/0014-4835(90)90103-2

    authors: Stribling D

    更新日期:1990-06-01 00:00:00

  • TIM2 modulates retinal iron levels and is involved in blood-retinal barrier breakdown.

    abstract::Careful control of iron availability in the retina is central to maintenance of iron homeostasis, as its imbalance is associated with oxidative stress and the progression of several retinopathies. Ferritin, known for its role in iron storage and detoxification, has also been proposed as an iron-transporter protein, th...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2020.108292

    authors: Valença A,Mendes-Jorge L,Bonet A,Catita J,Ramos D,Jose-Cunilleras E,Garcia M,Carretero A,Nacher V,Navarro M,Ruberte J

    更新日期:2021-01-01 00:00:00

  • Expression and distribution of MMPs and TIMPs in human uveal melanoma.

    abstract::Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) are involved in tumour invasion, metastasis and angiogenesis, and have been implicated as progression markers in uveal melanoma, although their topographical expression has not been fully described. In this study we compared the distribution and sp...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2008.03.010

    authors: Lai K,Conway RM,Crouch R,Jager MJ,Madigan MC

    更新日期:2008-06-01 00:00:00

  • Hexose transport in microvascular endothelial cells cultured from bovine retina.

    abstract::The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl-D-glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional gluc...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/0014-4835(83)90011-8

    authors: Betz AL,Bowman PD,Goldstein GW

    更新日期:1983-02-01 00:00:00

  • Method for isolating tight-binding inhibitors of rat lens aldose reductase.

    abstract::Numerous animal studies indicate that aldose reductase inhibitors (ARIs) are beneficial for the prevention or amelioration of diabetic complications such as neuropathy, nephropathy and the ocular complications of cataract, retinopathy and keratopathy. To aid in the identification of novel potent ARIs, we have previous...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2004.05.011

    authors: Sun G,Ma Y,Gao X,König S,Fales HM,Kador PF

    更新日期:2004-12-01 00:00:00

  • Low nitric oxide synthases (NOSs) in eyes with age-related macular degeneration (AMD).

    abstract::Nitric oxide (NO) production by vascular endothelium is important in regulation of blood flow. Reduced production of NO can adversely affect blood flow and other vascular functions. We investigated the expression of three nitric oxide synthase (NOS) isoforms in retina and choroid of aged human eyes and eyes with AMD. ...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2009.10.004

    authors: Bhutto IA,Baba T,Merges C,McLeod DS,Lutty GA

    更新日期:2010-01-01 00:00:00

  • A new model for limbal transplantation using E-GFP for follow-up of transplant survival.

    abstract::Limbal transplants in humans show a high rate of rejection even under local and systemic immunotherapy. In order to test immunomodulatory treatments a new limbal transplant model in the rat was developed using enhanced green fluorescent protein (E-GFP) as marker for follow-up. Sixty E-GFP-positive limbal transplants f...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2006.06.006

    authors: Keijser S,de Keizer RJ,Prins FA,Tanke HJ,van Rooijen N,Vrensen GF,Jager MJ

    更新日期:2006-11-01 00:00:00

  • Identification of low-Ca2+- and high-Ca2+-requiring neutral proteases in rat retina.

    abstract::Two low-Ca2+-requiring proteases (calpain I) and one high-Ca2+-requiring protease (calpain II) have been separated from the cytosol of rat retina by DEAE-cellulose column chromatography. Calpain I was half-maximally activated at 3 microM free Ca2+ and fully activated at 10 microM free Ca2+. Half-maximal activation of ...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/0014-4835(85)90098-3

    authors: Tsung PK,Lombardini JB

    更新日期:1985-07-01 00:00:00

  • A completely human-derived biomaterial mimicking limbal niche: Platelet-rich fibrin gel.

    abstract::Platelet-rich fibrin (PRF) is a natural biomaterial and has excellent biochemical and physical properties with a history of proven biocompatibility in the field of tissue engineering and regenerative medicine. Recent reports of fibrin-based matrices have offered new opportunities to apply PRF as a supplement for in vi...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2018.04.013

    authors: Dereli Can G,Akdere ÖE,Can ME,Aydın B,Cagil N,Gümüşderelioğlu M

    更新日期:2018-08-01 00:00:00

  • Interactions of thymic stromal lymphopoietin with TLR2 and TLR4 regulate anti-fungal innate immunity in Aspergillus fumigatus-induced corneal infection.

    abstract::Thymic stromal lymphopoietin (TSLP) is an interleukin 7 (IL-7)-like four helix bundle cytokine that plays diverse roles in the regulation of immune responses. In fungal infection, pattern recognition receptors (PRRs), including the cell surface Toll-like receptors (TLRs) and cytoplasmic NOD-like receptors, recognize p...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2019.02.020

    authors: Dai C,Wu J,Chen C,Wu X

    更新日期:2019-05-01 00:00:00

  • Complex carbohydrates at the ocular surface of the mouse: an ultrastructural and cytochemical analysis.

    abstract::Scanning electron microscopy (SEM) of mouse cornea and conjunctiva fixed with picric acid-paraformaldehyde-glutaraldehyde (PA-P-G) mixture revealed a thin layer of amorphous material covering the microvilli of the corneal surface cells. At the transmission electron microscopic (TEM) level, this layer of material stain...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/0014-4835(84)90111-8

    authors: Wells PA,Hazlett LD

    更新日期:1984-07-01 00:00:00

  • Investigation of the potential utility of a linomide analogue for treatment of choroidal neovascularization.

    abstract::The aim of this study was to test the selectivity, in-vivo effectiveness, and potential mechanism of action of a linomide analogue (N-phenyl-1,2-dihydro-4-hydroxyl-2-oxo-quinoline-3-carboxamide, Lin05) for inhibition of choroidal neovascularization. The selectivity of Lin05 was tested in cell proliferation assays with...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2010.10.013

    authors: Abdel-Rahman MH,Yang Y,Salem MM,Meadows S,Massengill JB,Li PK,Davidorf FH

    更新日期:2010-12-01 00:00:00

  • Status of caveolin-1 in various membrane domains of the bovine lens.

    abstract::Recent studies of the distribution and relative concentration of caveolin-1 in fractions of bovine lens epithelial and fiber cells have led to the novel concept that caveolin-1 may largely exist as a peripheral membrane protein in some cells. Caveolin-1 is typically viewed as a scaffolding protein for caveolae in plas...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2007.05.011

    authors: Cenedella RJ,Sexton PS,Brako L,Lo WK,Jacob RF

    更新日期:2007-10-01 00:00:00

  • AII amacrine cells express the MT1 melatonin receptor in human and macaque retina.

    abstract::AII amacrine cells are critical interneurons in the rod pathway of mammalian retina, active primarily in dim lighting conditions. Melatonin, a neuromodulator produced at night in the retina, is believed to induce retinal adaptation to dim lighting conditions in most vertebrate species examined to date, including human...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/s0014-4835(03)00123-4

    authors: Scher J,Wankiewicz E,Brown GM,Fujieda H

    更新日期:2003-09-01 00:00:00

  • Regional resistivity variations in lens homogenates.

    abstract::The response of the lens to changes in the distribution and concentration of electrolytes can be measured by characterizing the changes in resistivity that occur secondary to variations in intracellular composition. Regional intracellular cytoplasmic resistivities of lens fiber cells can be approximated by homogenates...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/s0014-4835(87)80164-1

    authors: McEwan JR,Farnsworth PN

    更新日期:1987-04-01 00:00:00

  • Biochemical studies on the effects of continuous light on the albino rat retina.

    abstract::The causal mechanism of light damage in the albino rat retina has been investigated. Male and female albino Wistar rats weighing 200-300 g and previously maintained in normal room lighting of approximately 15 hr light (0.85 +/- 0.05 X 10(-4) W cm-2) per 9 hr dark cycle were simultaneously exposed continuously to unifo...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/0014-4835(86)90012-6

    authors: Akpalaba CO,Oraedu AC,Nwanze EA

    更新日期:1986-01-01 00:00:00

  • Abdominal obesity linked to a longer cone-mediated dark-adaptation recovery time in healthy eyes.

    abstract::Obesity has been associated with abnormal lipid metabolism and with tissue hypoxia. Human Bruch's membrane (BrM) lipid deposits have been proposed to create a diffusion barrier to metabolic exchange between the choroid and photoreceptors, delaying the regeneration of photopigments. The speed of retinal dark adaptation...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2019.02.007

    authors: Puell MC,Fernandez-Balbuena AÁ

    更新日期:2019-04-01 00:00:00

  • The interactive effects of genetic polymorphisms within LFA-1/ICAM-1/GSK-3β pathway and environmental hazards on the development of Graves' opthalmopathy.

    abstract::The purpose of this investigation was to explore the combined effects of single nucleotide polymorphisms (SNPs) within LFA-1/ICAM-1/GSK-3β pathway and environmental hazards on susceptibility to Graves' opthalmopathy (GO) among a Chinese Han population. Altogether 305 GO patients and 283 Graves' disease (GD) subjects w...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2018.05.022

    authors: Yang G,Fu Y,Lu X,Wang M,Dong H,Li Q

    更新日期:2018-09-01 00:00:00

  • Retinal toxicity of intravitreal triamcinolone acetonide at high doses in the rabbit.

    abstract::In order to study acute retinal toxicity of intravitreal triamcinolone acetonide (TA) at high doses in an animal model, thirty New Zealand albino rabbits were injected with intravitreal TA. The animals were divided in five groups: Group 1 received an intravitreal injection of 0.1 mL balanced salt solution; Group 2, 0....

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2006.10.006

    authors: Ruiz-Moreno JM,Montero JA,Bayon A,Rueda J,Vidal M

    更新日期:2007-02-01 00:00:00

  • Effects of poly(2-hydroxyethyl methacrylate) and poly(vinyl-pyrrolidone) hydrogel implants on myopic and normal chick sclera.

    abstract::There has been generally little attention paid to the utilization of biomaterials as an anti-myopia treatment. The purpose of this study was to investigate whether polymeric hydrogels, either implanted or injected adjacent to the outer scleral surface, slow ocular elongation. White Leghorn (Gallus gallus domesticus) c...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2008.10.029

    authors: Su J,Iomdina E,Tarutta E,Ward B,Song J,Wildsoet CF

    更新日期:2009-03-01 00:00:00

  • Pharmacological activation of mGlu2/3 metabotropic glutamate receptors protects retinal neurons against anoxic damage in the goldfish Carassius auratus.

    abstract::We examined the expression of mGlu2/3 metabotropic glutamate receptors in the retina of the goldfish Carassius auratus. mGlu2/3 receptors were expressed in all retinal layers internal to the photoreceptor layer, particularly in the outer and inner nuclear layers. Although the goldfish brain is able to tolerate prolong...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2006.11.008

    authors: Beraudi A,Bruno V,Battaglia G,Biagioni F,Rampello L,Nicoletti F,Poli A

    更新日期:2007-03-01 00:00:00

  • Minimization of photooxidative insult to calf lens protein irradiated with near UV-light in the presence of pigmented glucosides derived from human lens protein.

    abstract::An aqueous solution of a pigmented glucoside associated with human lens protein, 2-amino-3-hydroxyacetophenone-O-beta-D-glucoside (AHA-Glc), was irradiated with near UV-light. The near UV-irradiated glucoside was shown to generate a much lower level of active species of molecular oxygen as compared to the level of the...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2004.09.004

    authors: Inoue A,Sasaki D,Satoh K

    更新日期:2004-12-01 00:00:00

  • A splice variant of arrestin from human retina.

    abstract::Retinal arrestin is known to participate in the quenching of phototransduction through binding to light-activated and phosphorylated rhodopsin. Recently, a splice variant of retinal arrestin was identified in bovine photoreceptors which could bind unphosphorylated photoactive rhodopsin. In this report, a splice varian...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1006/exer.1996.0069

    authors: Smith WC

    更新日期:1996-06-01 00:00:00

  • Modulation of human fibroblast activity by selected angiogenesis inhibitors.

    abstract::Tenon's fibroblast (TF) bleb encapsulation is a common cause of filtering surgery failure. Various pharmacologic agents have been used in an attempt to inhibit this response. The effects of three angiogenesis inhibitors were studied. AGM-1470, a fumagillin analog and hydrocortisone 21-phosphate (HC21P) complexed with ...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1006/exer.1994.1037

    authors: Wong J,Wang N,Miller JW,Schuman JS

    更新日期:1994-04-01 00:00:00

  • Mapping age-related elasticity changes in porcine lenses using bubble-based acoustic radiation force.

    abstract::Bubble-based acoustic radiation force aims to measure highly localized tissue viscoelastic properties. In the current investigation, acoustic radiation force was applied to laser-induced bubbles to measure age-related changes in the spatial distribution of elastic properties within in vitro porcine lenses. A potential...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2006.10.007

    authors: Erpelding TN,Hollman KW,O'Donnell M

    更新日期:2007-02-01 00:00:00

  • A novel murine model of Fusarium solani keratitis utilizing fluorescent labeled fungi.

    abstract::Fungal keratitis is a common disease that causes blindness. An effective animal model for fungal keratitis is essential for advancing research on this disease. Our objective is to develop a novel mouse model of Fusarium solani keratitis through the inoculation of fluorescent-labeled fungi into the cornea to facilitate...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2013.03.002

    authors: Zhang H,Wang L,Li Z,Liu S,Xie Y,He S,Deng X,Yang B,Liu H,Chen G,Zhao H,Zhang J

    更新日期:2013-05-01 00:00:00

  • Non-invasive in vivo measurement of ocular rigidity: Clinical validation, repeatability and method improvement.

    abstract::Ocular rigidity (OR) is thought to play a role in the pathogenesis of glaucoma, but the lack of reliable non-invasive measurements has been a major technical challenge. We recently developed a clinical method using optical coherence tomography time-lapse imaging and automated choroidal segmentation to measure the puls...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1016/j.exer.2019.107831

    authors: Sayah DN,Mazzaferri J,Ghesquière P,Duval R,Rezende F,Costantino S,Lesk MR

    更新日期:2020-01-01 00:00:00

  • Endothelial toxicity of ceftazidime in anterior chamber irrigation solution.

    abstract::Due to the high toxicity of aminoglycoside antibiotics, many authors prefer to use third generation cephalosporines in the prophylaxis and treatment of intraocular infections. The aim of the present study was to determine safe ceftazidime levels in anterior chamber irrigation solution. Twenty-two eyes of 12 white New ...

    journal_title:Experimental eye research

    pub_type: 杂志文章

    doi:10.1006/exer.1996.0167

    authors: Duch-Samper AM,Capdevila C,Menezo JL,Hurtado-Sarrió M

    更新日期:1996-12-01 00:00:00