Abstract:
:The passage of glucose within the cornea has been thought to occur by passive diffusion processes. However, corneal glucose concentration profiles have not been established to support this notion. While microfluorometric methods of metabolite assay typically have been used as a means of assessing regional brain metabolism, this unique methodology of tissue isolation and metabolite determination has not previously been applied to the cornea. Since this technique permits metabolite quantification on microgram-sized tissue samples, a co-ordinated corneal glucose concentration profile can be obtained. Tissue preparation consisted of liquid nitrogen freezing, cryo-sectioning, and freeze-drying, with storage at -20 degrees C. The sections were thawed under vacuum pump, subsectioned, weighed, and assayed for glucose concentration (by dry weight). This study established a glucose concentration profile of the epithelium, anterior stroma, midstroma, posterior stroma, and endothelium for the normal pigmented rabbit cornea. A glucose concentration profile for UV radiation-exposed rabbit corneas also was documented. The UV radiation glucose profile data indicate the presence of an active transport mechanism capable of delivering glucose into the corneal epithelium against a concentration gradient. The presence of a transport system that 'pulls' glucose through the deeper corneal layers thus would make epithelial integrity important for the maintenance of overall corneal viability.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Lattimore MR Jrdoi
10.1016/0014-4835(88)90037-1subject
Has Abstractpub_date
1988-11-01 00:00:00pages
699-704issue
5eissn
0014-4835issn
1096-0007journal_volume
47pub_type
杂志文章abstract::Diabetic retinopathy shares many characteristics features of a low grade chronic inflammatory disease. Its progression resists arrest when good metabolic control is re-established after a period of poor metabolic control, suggesting a 'metabolic memory' phenomenon. The aim of this study is to investigate the effect of...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.02.006
更新日期:2010-05-01 00:00:00
abstract::The influence of autonomic neurotransmitters on the growth and melanogenesis of cultured uveal melanocytes was studied. Uveal melanocytes were cultured with medium supplemented with cAMP elevating agents and basic fibroblast growth factor (complete medium). The cells were plated into multiple well plates, and various ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0869
更新日期:2000-09-01 00:00:00
abstract::In the neural retina, glial cells control formation of ionic gradients by mediating transmembrane water fluxes through aquaporin (AQP) water channels. Retinal content and immunolocalization of two water channels, AQP1 and AQP4, in the diabetic rat retinas during high-salt loading were examined in this study. Diabetes ...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2009-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(83)90009-x
更新日期:1983-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2015-09-01 00:00:00
abstract::Electrical field stimulation produced contractions of the rabbit isolated iris sphincter muscle which were completely blocked by tetrodotoxin (10(-6) M). Low frequency (0.16-5 Hz) responses were atropine-resistant, but were prevented by sensory (trigeminal) denervation, and were considered to be owing to antidromic st...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90099-x
更新日期:1984-02-01 00:00:00
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journal_title:Experimental eye research
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更新日期:2017-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.02.012
更新日期:2006-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2002.2058
更新日期:2002-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 信件
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0650
更新日期:1999-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1037
更新日期:1994-04-01 00:00:00
abstract::Rabbit immunoglobulin G (IgG) binds specifically to the human corneal epithelium. We investigated this phenomenon to identify the binding material in the tissue. Sections of human cornea were immunostained with negative-control grade rabbit IgG, F(ab')(2) (the antigen recognition fragment of IgG yielded by pepsin dige...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.01.006
更新日期:2004-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2017-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.03.021
更新日期:2005-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.10.015
更新日期:2010-02-01 00:00:00
abstract::This review summarizes the Ernst H. Bárány Prize Lecture given at the meeting of the International Society of Eye Research in Geneva 2002. In the paper the path from the author's early studies on neurogenic inflammation in the eye to the search for a suitable prostaglandin analogue for glaucoma treatment, and the deve...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2003.11.016
更新日期:2004-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90156-o
更新日期:1990-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0047
更新日期:1996-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1156
更新日期:1993-11-01 00:00:00
abstract::The protein phosphatase 2B inhibitor, FK506, is an immunomodulatory polypeptide that has neuroprotective properties, the mechanisms of which have not been elucidated. A possible mechanism may be phosphorylation-mediated regulation of glutamate transporter activity. In the present study, we investigated the effect of F...
journal_title:Experimental eye research
pub_type: 信件
doi:10.1016/j.exer.2010.04.011
更新日期:2010-08-01 00:00:00
abstract::Ischaemia/reperfusion contributes to the pathophysiological process of many retinal diseases. Previous studies have shown that retinal ischaemia/reperfusion mainly results in neuronal degeneration, including thinning of the retina, retinal ganglion cell death and reductions in electroretinography. A high-salt diet con...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107784
更新日期:2019-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0014-4835(05)80019-3
更新日期:1995-12-01 00:00:00
abstract::This study was designed to determine the safety and efficacy of transferrin-ricin A chain toxin (Tfr-rRA) at preventing retinal detachment in a rabbit model of proliferative vitreoretinopathy (PVR). The toxicity of intravitreal Tfr-rRA (1000-5000 ng) was determined by indirect ophthalmoscopy and electroretinography on...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0079
更新日期:1996-06-01 00:00:00
abstract::We examined the expression of mGlu2/3 metabotropic glutamate receptors in the retina of the goldfish Carassius auratus. mGlu2/3 receptors were expressed in all retinal layers internal to the photoreceptor layer, particularly in the outer and inner nuclear layers. Although the goldfish brain is able to tolerate prolong...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.11.008
更新日期:2007-03-01 00:00:00
abstract::Previous studies have shown that retinal damage induced by damaging light exposure is more severe in superior retina than inferior retina when measured along the vertical meridian of eyes. However, the extent of retinal damage over all retinal regions is not clear. Albino rats were exposed to bright light (5000 lux fo...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.06.002
更新日期:2008-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.12.013
更新日期:2005-06-01 00:00:00
abstract::The function of acetylcholinesterase (AChE) is to terminate the action of acetylcholine at the cholinergic synapse. Recent evidence suggests additional roles for acetylcholinesterase as a peptidase and/or a protease which is expressed by growing neurites as part of their invasion of developing neural structures. We re...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(95)80001-8
更新日期:1995-02-01 00:00:00