Abstract：
:The protein phosphatase 2B inhibitor, FK506, is an immunomodulatory polypeptide that has neuroprotective properties, the mechanisms of which have not been elucidated. A possible mechanism may be phosphorylation-mediated regulation of glutamate transporter activity. In the present study, we investigated the effect of FK506 on glutamate transporter localization and activity in the ischaemic mouse retina. FK506 did not appear to modulate the localization or activity of glutamate transporters under simulated ischaemic conditions. Our present data suggest that the mechanism by which FK506 exerts its neuroprotective action is not attributable to alterations in retinal glutamate transport.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Holcombe DJ,Gole GA,Barnett NLdoi
10.1016/j.exer.2010.04.011subject
Has Abstractpub_date
2010-08-01 00:00:00pages
315-9issue
2eissn
0014-4835issn
1096-0007pii
S0014-4835(10)00122-3journal_volume
91pub_type
信件abstract：:Liquid protein diets have been widely used in the United States to control weight gain. Pregnant rats were fed a standard casein diet in which liquid protein was substituted for the protein portion; all other necessary nutrients were supplied. The average incidence of cataracts in the offspring from mothers fed liquid...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90071-4
更新日期：1983-08-01 00:00:00
abstract：:Cysteine cathepsins are a family of proteases involved in intracellular protein turnover and extracellular matrix degradation. Cathepsin B (Ctsb) and cathepsin Z (Ctsz) promote tumorigenesis and Ctsb is a known modulator of tumor angiogenesis. We therefore investigated the angiomodulatory function of these cathepsins ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.06.014
更新日期：2013-10-01 00:00:00
abstract：:Lacritin is a mitogen of human salivary gland cells as well as a stimulator of human corneal epithelial cells. It is expected to be an important factor in maintaining the surrounding ocular surface. The monkey would be a relevant animal model in which to study the role of lacritin in ophthalmic physiology and patholog...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.07.019
更新日期：2007-11-01 00:00:00
abstract：:Iris pigment epithelial (IPE) cells from the anterior segment in the eye are able to suppress activation of bystander responder T cells in vitro. The cultured IPE cells fully suppress proliferation and cytokine production by responder T cells via direct cell-to-cell contact. We have now investigated whether primary cu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.01.011
更新日期：2009-06-01 00:00:00
abstract：:Colonization by Staphylococcus aureus (S. aureus) has been implicated in many infectious and wound healing disorders. This study was performed to characterize the pathogenic role of S. aureus alpha-hemolysin (alpha-toxin) in corneal epithelial wound healing and infectious keratitis in the setting of a corneal wound. T...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.02.019
更新日期：2019-04-01 00:00:00
abstract：:Steady state photoreceptor specific mRNA levels in bovine retina were studied during fetal maturation for five gene transcripts, including rhodopsin, arrestin (S-antigen), rod alpha-subunit of transducin, interphotoreceptor retinoid-binding protein (IRBP) and rod alpha-subunit of cGMP phosphodiesterase in order to und...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1034
更新日期：1993-03-01 00:00:00
abstract：:Sugar alcohols have been reported to accumulate in retinal pigment epithelium (RPE) of diabetic animals. This finding has raised interest in the role of RPE in diabetes-associated retinal changes such as cystoid macular edema. To confirm the presence of aldose reductase in this tissue, the NADPH-dependent enzyme was p...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1119
更新日期：1993-08-01 00:00:00
abstract：:The purpose of this research was to study the early histologic changes occurring in the lens during the formation of selenite overdose cataract. Fourteen-day-old rat pups received a single injection of 2.25 mg Se (kg body wt)-1. Lenses at each of three stages of cataract formation were observed biomicroscopically, and...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90077-6
更新日期：1985-04-01 00:00:00
abstract：:An experimental study was conducted on eight litters of newborn rats to evaluate the effects of supplemental oxygen administration on the retinal vasculature. The animals and their mothers were kept inside a pressure chamber and treated for the first 5 days of life. On the sixth day, they were removed and kept for fiv...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80179-3
更新日期：1987-03-01 00:00:00
abstract：:The lacrimal functional unit (LFU) is defined by the 2007 International Dry Eye WorkShop as 'an integrated system comprising the lacrimal glands, ocular surface (cornea, conjunctiva and meibomian glands) and lids, and the sensory and motor nerves that connect them'. The LFU maintains a healthy ocular surface primarily...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2008.09.002
更新日期：2009-05-01 00:00:00
abstract：:Innervated myotendinous cylinders (IMCs) in rabbit extraocular muscles (EOMs) were identified for the first time. The nature of IMC nerve terminals was demonstrated by means of electron microscopy and double fluorescent staining. The distal EOM portions of four rabbits of different age and sex were prepared for transm...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1085
更新日期：2001-12-01 00:00:00
abstract：:Following the identification of potent, orally active inhibitors of aldose reductase, the next challenge comes in demonstrating clinical utility in the treatment of diabetic complications. It is not feasible to conduct pivotal studies in all indications within the same trial and neuropathy evolved as the lead indicati...
journal_title：Experimental eye research
pub_type： 临床试验,杂志文章,评审
doi：10.1016/0014-4835(90)90103-2
更新日期：1990-06-01 00:00:00
abstract：:Fungal keratitis is a common disease that causes blindness. An effective animal model for fungal keratitis is essential for advancing research on this disease. Our objective is to develop a novel mouse model of Fusarium solani keratitis through the inoculation of fluorescent-labeled fungi into the cornea to facilitate...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.03.002
更新日期：2013-05-01 00:00:00
abstract：:This study sought to pharmacologically characterize bradykinin receptors on SV40-immortalized human trabecular meshwork (HTM3) cells. Phosphoinositide (PI) turnover studies were conducted using [3H]myo-inositol-labeled HTM3 cells and anion exchange chromatography to quantify [3H]inositol phosphates generated in respon...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0157
更新日期：1996-12-01 00:00:00
abstract：:This study evaluated the efficacy of Plasma rich in growth factors (PRGF-Endoret) on the corneal wound healing process after Photorefractive keratectomy (PRK). To address this, blood from three healthy donors was collected, centrifuged and, the whole plasma column (WP) and the plasma fraction with the highest platelet...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.07.007
更新日期：2013-10-01 00:00:00
abstract：:A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25 degrees C, as dedu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1175
更新日期：1993-12-01 00:00:00
abstract：:Although a range of changes in anterior segment structures have been documented to occur during accommodation, the quantification of changes in the structure of the anterior sclera during the accommodation process in human subjects has yet to be examined. This study therefore aimed to investigate the presence of short...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.07.023
更新日期：2018-12-01 00:00:00
abstract：:Bubble-based acoustic radiation force aims to measure highly localized tissue viscoelastic properties. In the current investigation, acoustic radiation force was applied to laser-induced bubbles to measure age-related changes in the spatial distribution of elastic properties within in vitro porcine lenses. A potential...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.10.007
更新日期：2007-02-01 00:00:00
abstract：:Eleven monoclonal antibodies against different segments of bovine rhodopsin were used with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal photoreceptor cells for visualization by transmission electron microscopy. Antibodies against the C-terminus, F1-F2 loop and N-terminus of rho...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(86)90017-5
更新日期：1986-01-01 00:00:00
abstract：:Use has been made of the specific interactions between monoclonal antibodies and the alpha A or alpha B subunits of alpha-crystallin to devise methods for the purification of the intact protein or its subunits. alpha A and alpha B subunits were separated by affinity chromatography on an immobilized monoclonal antibody...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80007-1
更新日期：1988-04-01 00:00:00
abstract：:Transport of circulating sulfur amino acids (SAA) into the lens epithelium and de novo glutathione (GSH) synthesis were studied in the perfused guinea-pig eye. Plasma-to-aqueous transfer of SAA was in their intact form (> or = 98%) and comparable with sucrose (an extracellular marker) within 30 min. The unidirectional...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0260
更新日期：1997-04-01 00:00:00
abstract：:The similarities between glaucoma and mitochondrial optic neuropathies have driven a growing interest in exploring mitochondrial function in glaucoma. The specific loss of retinal ganglion cells is a common feature of mitochondrial diseases - not only the classic mitochondrial optic neuropathies of Leber's Hereditary ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2010.07.015
更新日期：2011-08-01 00:00:00
abstract：:This study was conducted to evaluate the impact of varying scleral material properties on the biomechanical response of the cornea under air-puff induced deformation. Twenty pairs of human donor eyes were obtained for this study. One eye from each pair had its sclera stiffened using 4% glutaraldehyde, while the fellow...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107904
更新日期：2020-02-01 00:00:00
abstract：:Corneal epithelial (CE) cells are exposed to environmental insults (e.g., UV-irradiation), yet they suffer little damage. Our previous studies suggest that chicken CE cells have a novel form of protection involving having ferritin in a nuclear location where it can bind to DNA and sequester free iron. Here we describe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.02.002
更新日期：2016-04-01 00:00:00
abstract：:The function of acetylcholinesterase (AChE) is to terminate the action of acetylcholine at the cholinergic synapse. Recent evidence suggests additional roles for acetylcholinesterase as a peptidase and/or a protease which is expressed by growing neurites as part of their invasion of developing neural structures. We re...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(95)80001-8
更新日期：1995-02-01 00:00:00
abstract：:The hypothesis is advanced that the purpose of the eyeball movements during REM sleep is to stir the aqueous humor behind the closed lids and so avoid the risk that its stagnation could cause corneal anoxia. The relevance of the hypothesis to evolutionary biology and intensive care nursing is discussed. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0444
更新日期：1998-02-01 00:00:00
abstract：:Lens epithelium-derived growth factor (LEDGF) has been shown to enhance survival of lens epithelial cells (LECs) against stress. The objectives of these studies are to determine how LEDGF controls PKC gamma activity in normal LECs: how this control of PKC gamma regulates the phosphorylation of Connexin 43, the inhibit...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00049-6
更新日期：2003-05-01 00:00:00
abstract：:The P23H-1 rat strain carries a rhodopsin mutation frequently found in retinitis pigmentosa patients. We investigated the progressive degeneration of the inner retina in this strain, focussing on retinal ganglion cells (RGCs) fate. Our data show that photoreceptor death commences in the ventral retina, spreading to th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.10.003
更新日期：2010-12-01 00:00:00
abstract：:Lack of an optimal in vitro model of human corneal epithelial (HCE) cells is a major limitation in studying normal functions and gene regulations in HCE. Moreover, availability of a multi-layered HCE culture can reduce the usage of animals in the toxicity testing of consumer products. We have developed tetracycline-re...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00175-1
更新日期：2003-10-01 00:00:00
abstract：:Retinal stimulation with high spatial resolution requires close proximity of electrodes to target cells. This study examines the effects of material coatings and 3-dimensional geometries of subretinal prostheses on their integration with the retina. A trans-scleral implantation technique was developed to place microfa...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.09.018
更新日期：2009-01-01 00:00:00
