Abstract：
:Iris pigment epithelial (IPE) cells from the anterior segment in the eye are able to suppress activation of bystander responder T cells in vitro. The cultured IPE cells fully suppress proliferation and cytokine production by responder T cells via direct cell-to-cell contact. We have now investigated whether primary cultured human iris pigment epithelial (h-IPE) cells that were established from fresh iris tissues can also inhibit the activation of T cells in vitro. We found that cultured h-IPE cells significantly inhibited T cell proliferation and the IFN-gamma production by the target T cells from both the allogeneic and autogeneic peripheral blood mononuclear cells (PBMCs). The h-IPE cells also inhibited the activation of CD4(+) T cells from patients with active uveitis. The suppression by h-IPE occurred in a completely contact-dependent manner. The h-IPE constitutively expressed transforming growth factor beta (TGFbeta) and the receptors, and the T cells exposed to h-IPE greatly expressed Smad transcripts. In addition, TGFbeta2-siRNA transfected h-IPE failed to inhibit activation of responder T cells. Similarly, h-IPE cells in the presence of anti-TGFbeta neutralizing antibodies or recombinant TGFbeta receptor blocking proteins failed to inhibit the T-cell activation. In conclusion, cultured human iris pigment epithelium fully inhibits T cell activation in vitro. Our data support the hypothesis that the ocular resident cells play a critical role in immunosuppression in the eye.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Horie S,Sugita S,Futagami Y,Kawaguchi T,Kamoi K,Shirato S,Mochizuki Mdoi
10.1016/j.exer.2009.01.011subject
Has Abstractpub_date
2009-06-01 00:00:00pages
1033-42issue
6eissn
0014-4835issn
1096-0007pii
S0014-4835(09)00004-9journal_volume
88pub_type
杂志文章abstract：:This study sought to pharmacologically characterize bradykinin receptors on SV40-immortalized human trabecular meshwork (HTM3) cells. Phosphoinositide (PI) turnover studies were conducted using [3H]myo-inositol-labeled HTM3 cells and anion exchange chromatography to quantify [3H]inositol phosphates generated in respon...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0157
更新日期：1996-12-01 00:00:00
abstract：:The purpose of this study was to describe the distribution of Muller cell within the peripapillary retinal nerve fiber layer (RNFL) in human eyes. Eleven unpaired normal postmortem eyes were recruited into this study. Each eye was sectioned using the "umbrella technique" to obtain a concentric peripapillary ring cente...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.10.022
更新日期：2018-01-01 00:00:00
abstract：:The aqueous humor is produced by the epithelium of the ciliary body, a complex structure encircling the anterior segment of the eye. Aqueous humor production occurs by active transport, but the mechanism of this process is not understood. To produce a preparation in which active transport can be investigated, we have ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1156
更新日期：1993-11-01 00:00:00
abstract：:Cochlin deposits were observed in the trabecular meshwork (TM) of 8-month-old glaucomatous DBA/2J mice, coincident with the reported onset of increased intraocular pressure and optic nerve damage. An age-dependent increase in cochlin was observed up to 10 months of age and was paralleled by a decrease in type II colla...
journal_title：Experimental eye research
pub_type： 信件
doi：10.1016/j.exer.2005.01.028
更新日期：2005-05-01 00:00:00
abstract：:The routine cultivation of human corneal endothelial cells, with the view to treating patients with endothelial dysfunction, remains a challenging task. While progress in this field has been buoyed by the proposed existence of progenitor cells for the corneal endothelium at the corneal limbus, strategies for exploitin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.07.002
更新日期：2014-10-01 00:00:00
abstract：:The aim of the study was to investigate the molecular genetics of uveal melanoma (UM) metastases and correlate it with disease progression. Twelve pathologically confirmed UM metastases from 11 patients were included. Molecular genetic alterations in chromosomes 3 (including the BAP1 region), 8q, 6p, and 1p were inves...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.04.010
更新日期：2012-07-01 00:00:00
abstract：:Many ocular surface tests have been developed for the clinical diagnosis of keratoconjunctivitis sicca, and several have been used to 'validate' animal models of Sjogren's and non-Sjogren's dry eye. However, many of these tests themselves have not been systematically studied or standardized, and yet their use in anima...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2004.07.002
更新日期：2004-11-01 00:00:00
abstract：:Glutamate is an excitatory neurotransmitter in the central and peripheral nervous systems of the vertebrate. The previous studies show the presence of mRNAs of AMPA-type glutamate receptors, GluR1 and GluR2, in the optic tectum of the pigeon, suggesting glutamatergic input from the retina. The present study examined l...
journal_title：Experimental eye research
pub_type： 信件
doi：10.1016/j.exer.2009.03.024
更新日期：2009-09-01 00:00:00
abstract：:Previously, we demonstrated that the inwardly rectifying K(+) (Kir) channel subunit Kir7.1 is highly expressed in bovine and human retinal pigment epithelium (RPE). The purpose of this study was to determine whether any of the 14 other members of the Kir gene family are expressed in native human RPE. Conventional reve...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.05.010
更新日期：2008-09-01 00:00:00
abstract：:The shapes of two human lenses of different ages (27 and 46 years old) were altered by applying a stretching force to the ciliary muscle. The force was in the outward radial direction and was produced by an electronically driven stretching instrument which is described. The lenses were photographed for different degre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1168
更新日期：1993-11-01 00:00:00
abstract：:Docosahexaenoic acid is the major polyunsaturated fatty acid in photoreceptor membrane phospholipids and is thought to be necessary for photoreceptor function. Docosahexaenoic acid may be metabolized to docosanoids or, by retroconversion, to eicosapentaenoic acid followed by lipoxygenation and synthesis of n-3 metabol...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0116
更新日期：1996-09-01 00:00:00
abstract：:Previous studies have suggested that abnormal corneal wound healing in patients after photorefractive keratectomy (PRK) is associated with the appearance of myofibroblasts in the stroma between two and four weeks after surgery. The purpose of this study was to examine potential myofibroblast progenitor cells that migh...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.02.022
更新日期：2009-08-01 00:00:00
abstract：:The elimination of spermine, the end product of cellular polyamine, from the retina to the blood across the blood-retinal barrier (BRB) was investigated. The in vivo microdialysis study revealed that the elimination of [(3)H]spermine from vitreous humor after vitreous bolus injection was in a biexponential manner. The...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.05.002
更新日期：2014-07-01 00:00:00
abstract：:Platelet-rich fibrin (PRF) is a natural biomaterial and has excellent biochemical and physical properties with a history of proven biocompatibility in the field of tissue engineering and regenerative medicine. Recent reports of fibrin-based matrices have offered new opportunities to apply PRF as a supplement for in vi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.013
更新日期：2018-08-01 00:00:00
abstract：:This study aimed to assess the cytotoxic effect of low molecular weight components (LMWC) and conventional silicone oils (SOs) 1000 cSt with different degree of purification (raw, intermediate, and purified) using in vitro cytotoxicity tests. Direct contact cytotoxicity tests were performed in BALB 3T3 and human retin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108018
更新日期：2020-05-01 00:00:00
abstract：:Recently we determined that the Transient Receptor Potential Vanilloid 4 ion channel (TRPV4) has a crucial signaling role in a pathway that regulates various aspects of lens epithelium function. Here, we report on a different TRPV channel, TRPV1, in porcine lens. The presence of TRPV1 in the lens was evident from RT-P...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.006
更新日期：2018-07-01 00:00:00
abstract：:Type I and type II isoenzymes of cyclic AMP-dependent protein kinase were identified by anion exchange chromatography and immunoblotting in human retina at various stages of development. The type I form was typical of the early fetal stage during which proliferation and migration were the primary cellular processes. I...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90059-6
更新日期：1989-06-01 00:00:00
abstract：:Oxyradicals probably play a major role in a number of specific pathological conditions of intraocular tissues, such as cataract formation and retinal degeneration. This paper reviews some of the mechanistic concepts relating to tissue damage by highly reactive oxidants derived from endogenous precursors, hydrogen pero...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90075-7
更新日期：1984-12-01 00:00:00
abstract：:Transforming growth factor-beta (TGF-β) is one of the main epithelial-mesenchymal transition (EMT)-inducing factors. In general, TGF-β-induced EMT promotes cell migration and invasion. TGF-β also acts as a potent regulator of pericellular proteolysis by regulating the expression and secretion of plasminogen activators...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.06.020
更新日期：2013-10-01 00:00:00
abstract：:We have investigated the biochemical and cell biological basis of the reported beneficiary effects of the leaf extracts of the plant Ginkgo biloba, which has been used as a possible ophthalmic drug. The antioxidant, antimicrobial, anti-apoptotic and cytoprotective properties of the standardized extract called EGb761 w...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：
更新日期：2002-10-01 00:00:00
abstract：:The carbohydrate epitope Gal alpha 1-3Gal-R (alpha-galactosyl epitope), which is detectable by its binding with Bandeiraea simplicifolia-IB4 lectin, was found in glycosphingolipids (GSLs), both neutral and acidic (gangliosides), from lens tissues of non-primate mammals, but not in those of human senile cataracts and O...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1151
更新日期：1994-12-01 00:00:00
abstract：:In order to study acute retinal toxicity of intravitreal triamcinolone acetonide (TA) at high doses in an animal model, thirty New Zealand albino rabbits were injected with intravitreal TA. The animals were divided in five groups: Group 1 received an intravitreal injection of 0.1 mL balanced salt solution; Group 2, 0....
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.10.006
更新日期：2007-02-01 00:00:00
abstract：:Intraocular pressure (IOP) is the most critical risk factor for primary open angle glaucoma (POAG). In most cases of POAG, IOP is increased because of an abnormally high aqueous humor outflow resistance in the juxtacanalicular region of the trabecular meshwork. A distinct structural change in the trabecular meshwork o...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2009.01.005
更新日期：2009-04-01 00:00:00
abstract：:Photoreceptor outer segments (OS) in the vertebrate retina undergo a process of continual renewal involving shedding of disc membranes that are cleared by phagocytic uptake into the retinal pigment epithelium (RPE). In dystrophic Royal College of Surgeons (RCS) rats, OS phagocytosis is blocked by a mutation in the gen...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.08.006
更新日期：2015-11-01 00:00:00
abstract：:A potential role for myosin light chain kinase (MLCK) in regulating intraocular pressure and outflow function has recently been reported in living monkey eye and rabbit eye. There is little information about the effects of the ocular hypotensive agents, prostaglandin F2alpha (PGF2alpha) and latanoprost on this signali...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.10.015
更新日期：2004-02-01 00:00:00
abstract：:Research in animal models have shown that exposing the eye to positive or negative spectacle lenses can lead to predictable changes in eye growth. Recent research indicates that brief periods (1-2 h) of monocular defocus results in small, but significant changes in axial length and choroidal thickness of human subject...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.03.019
更新日期：2019-05-01 00:00:00
abstract：:Exposure to sulfur mustard (SM) may result in severe ocular injuries. While some of the eyes show a clinical resolution of the injury (defined as clinically non-impaired), part of the eyes develop irreversible late ocular pathologies (defined as clinically impaired) that may lead to corneal blindness. Understanding th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.04.011
更新日期：2019-07-01 00:00:00
abstract：:Binocular vision provides the most accurate and precise depth information; however, many people have impairments in binocular visual function. It is possible that other sensory inputs could be used to obtain reliable depth information when binocular vision is not available. However, it is currently unknown whether dep...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.08.013
更新日期：2019-06-01 00:00:00
abstract：:The results reported here clearly document changes in the properties and the organization of fiber-cell membrane lipids that occur with age, based on electron paramagnetic resonance (EPR) analysis of lens membranes of clear lenses from donors of age groups from 0 to 20, 21 to 40, and 61 to 80 years. The physical prope...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.01.018
更新日期：2015-03-01 00:00:00
abstract：:Glycoconjugate mucin secretion from conjunctival goblet cells is tightly regulated by nerves and specialized pro-resolving mediators (SPMs) to maintain ocular surface health. Here we investigated the actions of the SPM resolvin E1 (RvE1) on cultured rat conjunctival goblet cell glycoconjugate secretion and intracellul...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.04.015
更新日期：2018-08-01 00:00:00
