Abstract:
:Transport of circulating sulfur amino acids (SAA) into the lens epithelium and de novo glutathione (GSH) synthesis were studied in the perfused guinea-pig eye. Plasma-to-aqueous transfer of SAA was in their intact form (> or = 98%) and comparable with sucrose (an extracellular marker) within 30 min. The unidirectional transport rates (ml min-1 g-1) of 35S-labeled cystine, cystine and methionine into the epithelium were: 0.0057, 0.0003 and 0.0073 from plasma, and 1.41, 0.005 and 1.69 from aqueous, respectively. The unidirectional epithelial uptake was limited to 1 min for all three [35S]SAA, and the isotopic steady-state ratio was achieved between 1 and 30 min. Cortical uptake was time-dependent and progressive between 1 and 30 min, but undetectable within 1 min. The high performance liquid chromatography (HPLC) analysis of the epithelium revealed that following 1 min of unidirectional [35S]cysteine transport, 3% of the label was incorporated into GSH and > or = 95% was as cysteine. An average incorporation of [35S]cysteine into GSH within the 30 min period was 0.83% min-1 and 1%/min for the epithelium and cortex, respectively. Infusions of [35S]cystine and methionine failed to demonstrate incorporation into GSH. Maximal rates of de novo GSH epithelial synthesis were approximately 3 and 12 pmol g-1 from plasma and aqueous cysteine, respectively. A t1/2 of 5480 hr was estimated if epithelial GSH had to be replaced exclusively by synthesis from aqueous cysteine. Given the limited aqueous and epithelial cysteine pools, and low (from cysteine) or undetectable (from cystine and methionine) incorporation of the label into GSH, we conclude that de novo GSH synthesis from circulating and aqueous SAA can be only a minor source of the millimolar concentration of GSH in the epithelium.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Mackic JB,Kannan R,Kaplowitz N,Zlokovic BVdoi
10.1006/exer.1996.0260subject
Has Abstractpub_date
1997-04-01 00:00:00pages
615-26issue
4eissn
0014-4835issn
1096-0007pii
S0014-4835(96)90260-2journal_volume
64pub_type
杂志文章abstract::Stromal transparency is a critical factor contributing to normal function of the visual system. Corneal injury, surgery, disease and infection elicit complex wound healing responses that serve to protect against insults and maintain the integrity of the cornea, and subsequently to restore corneal structure and transpa...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2014.09.013
更新日期:2014-12-01 00:00:00
abstract::The following lectins: Con A, WGA, sWGA, PNA, RCA and UEA were used to study developmental changes in the expression of glycoconjugates during chicken lens morphogenesis. Con A, WGA, sWGA binding epitopes were observed in the lens placode and vesicle. Once the fiber mass was formed, the glycoconjugates were mainly fou...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0047
更新日期:1996-04-01 00:00:00
abstract::Axonal cytoskeleton proteins are intrinsically related to retinal ganglion cell (RGC) health and disease. This study quantifies and documents the sectoral distribution of axonal cytoskeleton proteins in the different laminar compartments of the normal human optic nerve head (ONH). Nine human eyes were used (mean age o...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.10.006
更新日期:2014-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.06.018
更新日期:2011-10-01 00:00:00
abstract::The chelating agent Na2EDTA was perfused through the anterior chambers of monkeys eyes in an attempt to find a concentration that would cause a rise in outflow facility without harming the cornea or anterior uvea. Na2EDTA (0.5 mM) proved effective in increasing the facility within 60 min. There were marked effects on ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80002-7
更新日期:1987-02-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2017-05-01 00:00:00
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1144
更新日期:1994-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0495
更新日期:1998-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(88)80042-3
更新日期:1988-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.07.009
更新日期:2006-03-01 00:00:00
abstract::The biosafety and efficiency of transplanting retinal pigment epithelial (RPE) cells derived from both human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) have been evaluated in phase I and phase II clinical trials. For further large-scale application, cryopreserved RPE cells must be used; th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108305
更新日期:2021-01-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2013.05.013
更新日期:2013-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80097-0
更新日期:1987-12-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2020.107981
更新日期:2020-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.05.011
更新日期:2007-10-01 00:00:00
abstract::Beta-crystallins are major protein constituents of the mammalian lens, where their stability and association into higher order complexes are critical for lens clarity and refraction. Dimerization is an initial step in formation of beta-crystallin complexes. Beta-crystallin association into dimers is energetically high...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2004.06.011
更新日期:2004-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.03.002
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journal_title:Experimental eye research
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doi:10.1016/j.exer.2005.09.018
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1015
更新日期:1993-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.03.009
更新日期:2007-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2015.11.023
更新日期:2016-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.01.004
更新日期:2005-07-01 00:00:00
abstract::Between 5 and 70% of genetic disease is caused by in-frame nonsense mutations, which introduce a premature termination codon (PTC) within the disease-causing gene. Consequently, during translation, non-functional or gain-of-function truncated proteins of pathological significance, are formed. Approximately 50% of all ...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2017.01.001
更新日期:2017-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.10.008
更新日期:2014-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90183-0
更新日期:1984-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,收录出版
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更新日期:2010-06-01 00:00:00
abstract::Intravitreal cidofovir has been shown to be a long acting and highly efficacious treatment for CMV retinitis; however decrease in IOP is an adverse effect. We wanted to determine the effect of cidofovir on intraocular pressure (IOP) in the guinea pig, and rabbit eye to develop an animal model of cidofovir induced ocul...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0273
更新日期:1997-05-01 00:00:00
abstract::This study evaluated the efficacy of Plasma rich in growth factors (PRGF-Endoret) on the corneal wound healing process after Photorefractive keratectomy (PRK). To address this, blood from three healthy donors was collected, centrifuged and, the whole plasma column (WP) and the plasma fraction with the highest platelet...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.07.007
更新日期:2013-10-01 00:00:00