Abstract：
:The objective of this study was to determine the cellular localization of P2Y(2) receptor gene expression in rabbit and primate ocular tissues using the technique of non-isotopic in situ hybridization. Fresh frozen whole eye from a New Zealand White rabbit and whole eye and eyelid from a rhesus macaque were cut into 5 microm thick sections and mounted onto glass slides. In situ hybridization was performed on ocular cryosections using digoxigenin-labeled P2Y(2) receptor riboprobes. Alkaline phosphatase-conjugated anti-digoxigenin antibody was used to localize riboprobe hybridization, which was subsequently visualized by staining with a precipitating alkaline phosphatase substrate. Cytoplasmic staining indicative of antisense riboprobe hybridization to P2Y(2) receptor mRNA was observed in the palpebral and bulbar conjunctival epithelium, including goblet cells, the corneal epithelium, and in meibomian gland sebaceous and ductal cells. Staining was also observed in both layers of the ciliary body epithelium, subcapsular epithelium of the lens, and corneal endothelium. In the posterior eye, staining was observed in various layers of the retina, including ganglion cell, inner nuclear, inner segment and retinal pigment epithelium layers, in the optic nerve head, and in a variety of structures within the choroid. No specific staining of sense riboprobe was seen on any of the ocular structures. These results demonstrate that the P2Y(2) receptor gene is expressed in a variety of ocular cells types and suggest that P2Y(2) receptors are associated with diverse physiological functions throughout the eye.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Cowlen MS,Zhang VZ,Warnock L,Moyer CF,Peterson WM,Yerxa BRdoi
10.1016/s0014-4835(03)00068-xkeywords:
subject
Has Abstractpub_date
2003-07-01 00:00:00pages
77-84issue
1eissn
0014-4835issn
1096-0007pii
S001448350300068Xjournal_volume
77pub_type
杂志文章abstract：:Aldose reductase (AR) is implicated in some of the disabling complications of diabetes, including neuropathy, retinopathy and cataracts. Our studies are aimed at further clarifying the role of AR in diabetes and facilitating the design of new classes of potent, specific AR inhibitors by gaining an understanding of the...
journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1983-12-01 00:00:00
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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journal_title：Experimental eye research
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doi：10.1016/j.exer.2007.01.021
更新日期：2007-06-01 00:00:00
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journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0883
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90007-9
更新日期：1989-05-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1995-10-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.02.001
更新日期：2008-05-01 00:00:00
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journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108297
更新日期：2021-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1985-07-01 00:00:00
abstract：:Accumulation of specific deposits and extracellular molecules under the retinal pigment epithelium (RPE) has been previously observed in eyes with age-related macular degeneration (AMD) and may play a role in the pathogenesis of AMD. Even though age is the major determinant for developing AMD, clinical studies have re...
journal_title：Experimental eye research
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doi：10.1016/j.exer.2009.02.020
更新日期：2009-06-15 00:00:00
abstract：:A proteinase from human lens membrane was purified by a procedure previously developed for a similar proteinase from bovine lenses (Srivastava, 1988a). The purification of the human proteinase was achieved by solubilization of the enzyme from membranes with 2% sodium deoxycholate followed by two consecutive passages t...
journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1989-02-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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更新日期：2016-09-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1006/exer.1996.0004
更新日期：1996-01-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1006/exer.1996.0265
更新日期：1997-05-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2003.09.003
更新日期：2004-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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journal_title：Experimental eye research
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doi：10.1016/j.exer.2007.11.011
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abstract：:Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) were isolated from frog retinal rod outer segment (ROS) membranes and their major molecular species determined. Saturated fatty acids are predominantly located on position-1 and polyunsaturates on position-2, although this assignment is not absolute. One-fifth...
journal_title：Experimental eye research
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doi：10.1016/0014-4835(83)90075-1
更新日期：1983-08-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1016/j.exer.2014.02.003
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abstract：:The influence of autonomic neurotransmitters on the growth and melanogenesis of cultured uveal melanocytes was studied. Uveal melanocytes were cultured with medium supplemented with cAMP elevating agents and basic fibroblast growth factor (complete medium). The cells were plated into multiple well plates, and various ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0869
更新日期：2000-09-01 00:00:00
abstract：:Axonal cytoskeleton proteins are intrinsically related to retinal ganglion cell (RGC) health and disease. This study quantifies and documents the sectoral distribution of axonal cytoskeleton proteins in the different laminar compartments of the normal human optic nerve head (ONH). Nine human eyes were used (mean age o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.10.006
更新日期：2014-11-01 00:00:00
abstract：:Changes in activities of ascorbate free radical (AFR) reductase (NADH:AFR oxidoreductase) and H2O2-dependent NADH oxidation were correlated with levels of insoluble protein in senile cataractous human lenses. The H2O2-dependent NADH oxidation activity was measured to reflect the content of free glutathione. AFR reduct...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90128-h
更新日期：1990-06-01 00:00:00
