Abstract：
:Two low-Ca2+-requiring proteases (calpain I) and one high-Ca2+-requiring protease (calpain II) have been separated from the cytosol of rat retina by DEAE-cellulose column chromatography. Calpain I was half-maximally activated at 3 microM free Ca2+ and fully activated at 10 microM free Ca2+. Half-maximal activation of calpain II was at 0.4 mM free Ca2+ while full activation was observed at 0.8 mM free Ca2+. Calpain activity has also been demonstrated in sealed rod outer segments. The soluble fraction of the rod outer segments contained 89% of the enzyme activity. The possible role of calpain in retina is discussed.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Tsung PK,Lombardini JBdoi
10.1016/0014-4835(85)90098-3subject
Has Abstractpub_date
1985-07-01 00:00:00pages
97-103issue
1eissn
0014-4835issn
1096-0007pii
0014-4835(85)90098-3journal_volume
41pub_type
杂志文章abstract：:Hypoxia-inducible factor (HIF) signaling cascade plays a critical role in angiogenesis by activating the transcription of genes encoding angiogenic growth factors. This study evaluated the effects of YC-1, a HIF-1 inhibitor, on the morphological, biochemical and molecular changes in human retinal microvascular endothe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.06.013
更新日期：2009-11-01 00:00:00
abstract：:We examine the immunoreactivity of the non-receptor tyrosine kinase, c-Src kinase and its downstream molecule, signal transducer and activator of transcription 3 (pStat3) in retinoblastoma (RB), and correlation with invasiveness and differentiation. Tumor samples from 40 patients with RB were available for the study. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.02.011
更新日期：2006-10-01 00:00:00
abstract：:A key model for examining the activity-dependent development of primary visual cortex (V1) involves the imbalance in activity between the two eyes induced by monocular deprivation (MD). MD early in life causes dramatic changes in the functional and structural organization of mammalian visual cortex. The molecular sign...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.12.016
更新日期：2013-04-01 00:00:00
abstract：:Peroxisomes were localized in the head of wild-type and mutant strains of Drosophila melanogaster by use of a cytochemical method for the demonstration of D-amino acid oxidase activity. With similar techniques we had found previously that vertebrate photoreceptors have few, if any, bodies with cytochemically demonstra...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90092-9
更新日期：1990-11-01 00:00:00
abstract：:Anatomic and physiological barriers limit drug delivery to the posterior segment of the eye via topical or systemic administration. Intravitreal administration has proven to be a safe and effective means of treating various posterior segment diseases. Elimination of a compound from the vitreous chamber may depend on l...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00311-1
更新日期：2003-03-01 00:00:00
abstract：:The gene for microphthalmia in the homozygous cinnamon mouse produces colobomatous microphthalmia due to failure of closure of the optic fissure. Optic fissure formation and closure were examined in control cinnamon and homozygous microphthalmic foetuses from the 10th to the 13th gestational day using light and electr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90093-6
更新日期：1989-08-01 00:00:00
abstract：:Recent studies have shown that lactate coupled water flux is the underlying mechanism of the corneal endothelial pump, which is highly dependent on the presence of bicarbonate. In this study we test the hypothesis that the increased intracellular pH (pHi) caused by bicarbonate stimulates glycolytic activity and the pr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108193
更新日期：2020-10-01 00:00:00
abstract：:Human retinal pigment epithelial cells in culture demonstrate adenylate cyclase activity. It is membrane-bound and modulated by GTP regulatory proteins. It is effectively activated only by beta-adrenergic agonists (L-isoproterenol greater than or equal to L-epinephrine greater than L-norepinephrine) and some prostagla...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80158-6
更新日期：1987-04-01 00:00:00
abstract：:Although it is important to investigate the effects of normal aging on the ocular circulation, few studies have examined the effects of aging on the retinal microcirculation in healthy subjects. The purpose of this study was to evaluate the effects of aging on the retinal microcirculation in healthy men. Forty-five he...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.07.019
更新日期：2009-12-01 00:00:00
abstract：:Transmural electrical properties of the isolated rabbit iris-ciliary body (I-CB) were measured in Ussing-Zerahn-type chambers. Control p.d. across the preparation was -1.2 +/- 0.1 mV, with the ciliary process (aqueous)-side consistently negative with respect to the ciliary body (blood)-side and the short-circuit curre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90096-4
更新日期：1984-02-01 00:00:00
abstract：:Mice are now routinely utilized in studies of aqueous humor outflow dynamics. In particular, conventional aqueous outflow facility (C) is routinely measured via perfusion of the aqueous chamber by a number of laboratories. However, in mouse eyes perfused ex-vivo, values for C are variable depending upon whether the pe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.08.011
更新日期：2017-11-01 00:00:00
abstract：:An investigation was undertaken to expand the data base for elemental concentrations within eye tissues of different species. This report provides data on the distribution of calcium, copper, iron and zinc in human, dog, bovine, bird, amphibian and fish ocular tissues. The variation between different eyes of the same ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90138-0
更新日期：1983-12-01 00:00:00
abstract：:A proteinase from human lens membrane was purified by a procedure previously developed for a similar proteinase from bovine lenses (Srivastava, 1988a). The purification of the human proteinase was achieved by solubilization of the enzyme from membranes with 2% sodium deoxycholate followed by two consecutive passages t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80067-3
更新日期：1989-02-01 00:00:00
abstract：:Developing canine retina, fixed in Bouin's or Tellyesnizky's acetic alcoholic formalin, was examined with a battery of biotinylated lectins. ConA and WGA consistently bound to all retinal layers including the tapetum cellulosum, retinal pigment epithelium, inner and outer segments, plexiform layers and ganglion cells....
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90150-s
更新日期：1990-10-01 00:00:00
abstract：:To determine the effect of roscovitine, a potent antiviral in tissue culture administered intramuscularly to rabbits or by eye drops to mice for the treatment of herpetic keratitis this study was commenced.New Zealand white rabbits infected with McKrae strain herpesvirus (HSV-1) were treated twice a day with 10mg Rosc...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00056-3
更新日期：2003-06-01 00:00:00
abstract：:Previous studies have shown that corneal endothelial cells contain mRNA and protein of various growth factors. However, the role of these endogenous growth factors in corneal endothelial wound healing is not fully elucidated. In the present study, we investigated the role of endogenous factors and several growth facto...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0918
更新日期：2000-12-01 00:00:00
abstract：:Simultaneous non-invasive visualization of blood vessels and nerves in patients can be obtained in the eye. The retinal vasculature is a target of many retinopathies. Inflammation, readily manifest by leukocyte adhesion to the endothelial lining, is a key pathophysiological mechanism of many retinopathies, making it a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.12.002
更新日期：2014-03-01 00:00:00
abstract：:The early events that occur rapidly after injury trigger signal cascades that are essential for proper wound closure of corneal epithelial cells. We hypothesize that injury releases ATP, which stimulates purinergic receptors and elicits the phosphorylation of epidermal growth factor receptor (EGFR) tyrosine residues a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.03.009
更新日期：2007-07-01 00:00:00
abstract：:In order to determine whether neuronal cell death in the retina accompanies senescence, retinas from pigmented ACI rats of various ages were analysed for changes in dimensions, cell densities, DNA content, and rhodopsin levels. Between the ages of 4 and 30 months, there were significant declines in the densities of nu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(86)90022-9
更新日期：1986-04-01 00:00:00
abstract：:Metabolomics studies in the retina and retinal pigment epithelium (RPE) in animal models or postmortem donors are essential to understanding the retinal metabolism and to revealing the underlying mechanisms of retinal degenerative diseases. We have studied how different methods of euthanasia (CO2 or cervical dislocati...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.05.032
更新日期：2018-09-01 00:00:00
abstract：:The spontaneously hypertensive rats (SHR) show a lower intraocular pressure--despite high arterial blood pressure--than the normotensive Wistar Kyoto rats (NR). In SHR the quantitative evaluation of resin casts and flat-mount preparations of the blood vessels of the ciliary body and choroid showed a significant dilati...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90004-1
更新日期：1985-02-01 00:00:00
abstract：:Crystalline lenses excised from 5-7-month-old rabbits and pigs were extracted for lipids with chloroform/methanol, 2/1 using the Folch method. The extracted crude lipids were analyzed at 202 MHz by 31P-NMR spectroscopy. Twelve membrane phospholipids were detected. Both rabbit and pig phospholipid profiles contained ph...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90207-b
更新日期：1990-03-01 00:00:00
abstract：:Previous studies suggest that an alpha 2-related isoform of the catalytic subunit is predominant in the lens fiber cells. The alpha 1 isoform is predominant in the lens epithelium (Garner, Horwitz and Enomoto, 1992). Data are presented to show that strophanthidin-sensitive K+ transport is sustained by both of these le...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1068
更新日期：1994-06-01 00:00:00
abstract：:Congenic hereditary cataract mice, BALB/c-nct/nct, were established by introducing the nct gene from Nakano into BALB/c mice. These mice developed a milder cortical form of cataract which developed sporadically and later in life than in Nakano mice. Combined use of BALB/c and BALB/c-nct/nct mice enables biochemical co...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90049-k
更新日期：1991-05-01 00:00:00
abstract：:Ischaemia/reperfusion contributes to the pathophysiological process of many retinal diseases. Previous studies have shown that retinal ischaemia/reperfusion mainly results in neuronal degeneration, including thinning of the retina, retinal ganglion cell death and reductions in electroretinography. A high-salt diet con...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107784
更新日期：2019-11-01 00:00:00
abstract：:Prostaglandins are widely used to lower intraocular pressure (IOP) as part of the treatment regimen for glaucoma. While FP and EP2 agonists are known to lower IOP, we investigated the ocular hypotensive activity and ocular drug distribution of PF-04475270, a novel EP4 agonist following topical administration in normot...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.05.002
更新日期：2009-11-01 00:00:00
abstract：:In chickens, transient changes in choroidal thickness are found in conditions in which the eye is slowing its growth in response to visual episodes that prevent excessive elongation. To test the hypothesis that the choroidal and ocular growth responses are linked, we used a variety of "brief daily" stimuli known to am...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.01.017
更新日期：2007-05-01 00:00:00
abstract：:The wound-healing response is critical to the outcome of refractive surgery and studying wound healing contributes to an understanding of the pathophysiology of other corneal injuries. Animal models allow research to be conducted with sufficient samples and under controlled parameters. We studied the hen to determine ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.02.017
更新日期：2006-10-01 00:00:00
abstract：:Retinal degenerations, regardless of the initiating event or gene defect, often result in a loss of photoreceptors. This formal deafferentation of the neural retina eliminates the intrinsic glutamatergic drive of the sensory retina and, perhaps more importantly, removes coordinated Ca++-coupled signaling to the neural...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2005.03.006
更新日期：2005-08-01 00:00:00
abstract：:Melanopsin is a non-image forming photoreceptor known to be present in the retina and it is considered to have light regulated tasks among other functions. In the present work, melanopsin presence in human lens epithelial cells as well as in human lens tissue is described for the first time. Moreover, studying the con...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.11.019
更新日期：2017-01-01 00:00:00
