Abstract:
:The integrity of the retinal pigment epithelium, especially that of the macula is essential for the preservation of vision into old age. The chronic exposure to sunlight and peroxidized lipids from phagocytized photoreceptor outer segments imposes a high level of oxidative stress on the retinal tissues, which increases with age as antioxidant protection declines and therefore could accelerate apoptosis. Bcl-2 known to facilitate mitochondrial DNA repair and cellular survival in other tissues was overexpressed in a single clone of human retinal pigment epithelium cells after stable transfection with humanbcl-2 in rhoSFV-neoexpression factor. Near confluent cells (2nd-4th generation permanently bcl-2 transfected) were protected from mitochondrial dysfunction after exposure to H(2)O(2) up to 150 microM. With 200 microM H(2)O(2), function in transfected cells declined by only 25% control activity as determined by MTT reduction assays, compared to wild type and vector only transfected cells expressing normal bcl-2 levels. Similarly the bcl-2 -transfected cells were more resistant to mitochondrial DNA damage after H(2)O(2) treatment than the other groups and suffered 50% less damage after exposure to 200 microM H(2)O(2), as assayed by quantitative polymerase chain reaction assays. These data suggest that bcl-2 overexpression protects human RPE cells from mitochondrial respiratory dysfuction, mitochondrial DNA damage and promotes cellular survival in response to oxidative stress induced by H(2)O(2).
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Godley BF,Jin GF,Guo YS,Hurst JSdoi
10.1006/exer.2001.1146keywords:
subject
Has Abstractpub_date
2002-06-01 00:00:00pages
663-9issue
6eissn
0014-4835issn
1096-0007pii
S0014483501911467journal_volume
74pub_type
杂志文章abstract::Misregulation of the innate immune response and other immune-related processes have been suggested to play a critical role in the pathogenesis of a number of different neurodegenerative diseases, including age related macular degeneration. In an animal model for photoreceptor degeneration, several genes of the innate ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.08.017
更新日期:2007-01-01 00:00:00
abstract::The supply of L-carnosine, a bioactive dipeptide of β-alanine and l-histidine, to the retina across the blood-retinal barrier (BRB) was studied. The in vivo and in vitro studies revealed low uptake activities for [(3)H]Gly-Sar, a representative dipeptide, suggesting that l-carnosine transport plays only a minor role a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.06.002
更新日期:2013-08-01 00:00:00
abstract::Long living animal models of retinitis pigmentosa (RP) can provide important information on the retinal changes that occur at the late stages of photoreceptor degeneration. The rhodopsin Pro347Leu transgenic rabbit (P347L Tg) is a model of RP, and it has been used to analyze the functional and morphological changes in...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.04.026
更新日期:2019-07-01 00:00:00
abstract::The Purkinje cell degeneration (pcd) mutant mouse rapidly loses cerebellar Purkinje cells and about 50% of its retinal photoreceptor cells at between 3 and 5 weeks of age, and thereafter slowly loses the remaining photoreceptor cells during the first year of life. An ultrastructural study of the developing photorecept...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90019-o
更新日期:1992-05-01 00:00:00
abstract::Recent studies have shown that lactate coupled water flux is the underlying mechanism of the corneal endothelial pump, which is highly dependent on the presence of bicarbonate. In this study we test the hypothesis that the increased intracellular pH (pHi) caused by bicarbonate stimulates glycolytic activity and the pr...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108193
更新日期:2020-10-01 00:00:00
abstract::The aim of the study was to investigate the molecular genetics of uveal melanoma (UM) metastases and correlate it with disease progression. Twelve pathologically confirmed UM metastases from 11 patients were included. Molecular genetic alterations in chromosomes 3 (including the BAP1 region), 8q, 6p, and 1p were inves...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.04.010
更新日期:2012-07-01 00:00:00
abstract::Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. As a consequence of the unc...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.11.004
更新日期:2012-01-01 00:00:00
abstract::The cornea receives the densest sensory innervation of the body, which is exclusively from small-fiber nociceptive (pain-sensing) neurons. These are similar to those in the skin of the legs, the standard location for neurodiagnostic skin biopsies used to diagnose small-fiber peripheral polyneuropathies. Many cancer ch...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.05.016
更新日期:2013-08-01 00:00:00
abstract::The purpose of this investigation is to characterize parvalbumin-immunoreactive (IR) neurons in the inner nuclear layer (INL) of zebrafish retina through immunocytochemistry, quantitative analysis, and confocal microscopy. In the INL, parvalbumin-IR neurons were located in the inner marginal portion of the INL. On the...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.11.014
更新日期:2009-03-01 00:00:00
abstract::Twenty-three human lenses from age 3-76 years were studied. Lenses obtained 24-36 post-mortem were exposed to monochromatic light (435.8 and 546.1 nm) in the I parallel and I+ modes and to white (tungsten lamp) unpolarized radiation. The light scattering intensities were obtained as a function of scattering angles fro...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90089-2
更新日期:1985-07-01 00:00:00
abstract::The Royal College of Surgeons (RCS) rat is an established animal model of retinitis pigmentosa, a family of inherited retinal diseases which starts with loss of peripheral vision and progresses to eventual blindness. Blood flow (BF), an important physiological parameter, is intricately coupled to metabolic function un...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.06.006
更新日期:2012-08-01 00:00:00
abstract::We studied a new fluorophotometric method to determine trabecular outflow facility in man. Pilocarpine was selected to evaluate this non-invasive method, since its primary ocular hypotensive effect is to increase trabecular outflow facility. Levobunolol was used to decrease intraocular pressure and aqueous humor flow ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90004-3
更新日期:1989-05-01 00:00:00
abstract::The histopathological features of uveitis and retinal vasculitis in acute experimental allergic encephalomyelitis (EAE) were investigated using light and electron microscopy. Lewis rats were immunized by spinal cord homogenate, complete Freund's adjuvant and Bordetella pertussis. The eyes of rats with EAE exhibited va...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1024
更新日期:1993-02-01 00:00:00
abstract::Human retinal pigmented epithelial (RPE) cells cultured on plastic, unlike RPE in situ, often fail to density arrest, do not produce melanin, and do not express mRNA for cellular retinaldehyde binding protein (CRALBP). When human RPE are cultured on the extracellular matrix produced by bovine corneal endothelial cells...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1158
更新日期:1993-11-01 00:00:00
abstract::Although it is important to investigate the effects of normal aging on the ocular circulation, few studies have examined the effects of aging on the retinal microcirculation in healthy subjects. The purpose of this study was to evaluate the effects of aging on the retinal microcirculation in healthy men. Forty-five he...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.07.019
更新日期:2009-12-01 00:00:00
abstract::Purified retinal pigment epithelial cells of bovine eyes have been fractionated by a series of buffer and detergent extractions. The electropherogram of the buffer-insoluble, Triton X-100-soluble fraction (RPE-TS) exhibits a major polypeptide band of M(r) 65 kDa and a variety of minor components. Electrophoretically p...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90008-g
更新日期:1992-12-01 00:00:00
abstract::Tissue transglutaminase (tTG) is a Ca(2+)-dependent enzyme catalyzing the formation of covalent crosslinks between peptide-bound glutamine and lysine residues. Lens crystallins, including alphaB-crystallin and several beta-crystallins, are in vitro substrates for tTG. In both human and bovine fetal lens extracts treat...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.11.011
更新日期:2008-02-01 00:00:00
abstract::Analysis of bovine interphotoreceptor matrix and conditioned medium from human Y-79 retinoblastoma cells by gelatin SDS-PAGE zymography reveals abundant activity of a 72-kDa M(r) gelatinase. The 72-kDa gelatinase from either source is inhibited by EDTA but not aprotinin or NEM, indicating that it is a metalloproteinas...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1107
更新日期:1994-09-01 00:00:00
abstract::The presence of eumelanin and pheomelanin in irides from eyes of various colors was determined and quantified by a highly specific microanalytical procedure based on chemical degradation. Significant differences in the type of melanin were observed in the stroma and iris pigment epithelium (IPE) fractions obtained by ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0518
更新日期:1998-09-01 00:00:00
abstract::Glaucoma is a leading cause of irreversible blindness worldwide. Lowering intraocular pressure (IOP) is the only strategy documented to delay the appearance and retard the progression of vision loss. One major approach for lowering IOP is to slow the rate of aqueous humor formation by the ciliary epithelium. As discus...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2008.11.005
更新日期:2009-04-01 00:00:00
abstract::The effect of a 'soft' anti-inflammatory corticosteroid on the corneal wound healing was investigated in rabbits. Albino rabbits were treated with soft steroid and dexamethasone after linear perforating corneal incision. Soft drug design is aimed to control and direct the drug activity and in vivo metabolism to nontox...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90229-n
更新日期:1990-02-01 00:00:00
abstract::Transport of circulating reduced glutathione (GSH) was studied at the basolateral side of the lens epithelium by using an in situ vascular eye perfusion technique in guinea-pigs with rapid sampling to ensure detecting initial uptakes. The unidirectional transport rates of [35S]-GSH (4 nM) from plasma and aqueous into ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0004
更新日期:1996-01-01 00:00:00
abstract::The retinoschisin protein is encoded on the short arm of the X-chromosome by RS1, is expressed abundantly in photoreceptor inner segments and in bipolar cells, and is secreted as an octamer that maintains the structural integrity of the retina. Mutations in RS1 lead to X-linked retinoschisis (XLRS), a disease characte...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108344
更新日期:2021-01-01 00:00:00
abstract::Adipose-Derived Stem Cells (ADSCs) have an important contribution in regenerative medicine ranging from testing stem cell therapy for disease treatment in pre-clinical models to clinical trials. For immediate use of stem cells for therapy, there is a requirement of the high dose of stem cells at different time points ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107860
更新日期:2019-12-01 00:00:00
abstract::Corneal mycotic ulceration is predominantly due to Aspergillus and Fusarium solani infection in tropical countries. In this study, we examined the proteome profile of tear samples from A. flavus keratitis patients at various stages of infection. The proteome was profiled using 2D PAGE and the protein levels were quant...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107700
更新日期:2019-09-01 00:00:00
abstract::Epithelial cells in explants from rat lenses differentiate into fibre cells when cultured with retina conditioned medium (RCM). This differentiation is characterised by cell elongation, the accumulation of alpha-crystallin and the onset of synthesis of the fibre specific beta- and gamma-crystallins. To determine the e...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(88)80083-6
更新日期:1988-02-01 00:00:00
abstract::microPx-11, a ferriheme undecapeptide proteolytic degradation product of cytochrome C is shown to be a peroxidase with broad specificity degrading H2O2 and tertiary butyl hydroperoxide. It is also capable of effectively eliminating superoxide and hydroxyl radical. The peroxidase loses activity in the presence of perox...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0336
更新日期:1997-10-01 00:00:00
abstract::Dexamethasone is a glucocorticoid that is widely used in the ophthalmic arena. The recent FDA approved dexamethasone implant can provide a three month efficacy but with high rate of drug related cataract and high intraocular pressure (IOP). It seems that higher steroid in aqueous humor and around lens may be associate...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.11.002
更新日期:2014-12-01 00:00:00
abstract::Retinal degeneration (RD) results from photoreceptor apoptosis. Cell transplantation, one potential therapeutic approach, requires expandable stem cells that can form mature photoreceptors when differentiated. Freshly dissociated primary retinal cells from postnatal day 2-6 (PN2-6) mouse retina can give rise, post-tra...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.07.003
更新日期:2010-10-01 00:00:00
abstract::The goal of this study was to investigate the efflux of K(+) from human corneal limbal epithelial cells (HCLE) exposed to ambient levels of UVB, which is known to cause apoptosis, and to examine the effect of K(+) channel blockers on loss of potassium induced by UVB. HCLE cells were exposed to 100-200 mJ/cm(2) UVB, fo...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.02.019
更新日期:2011-05-01 00:00:00