Abstract：
:microPx-11, a ferriheme undecapeptide proteolytic degradation product of cytochrome C is shown to be a peroxidase with broad specificity degrading H2O2 and tertiary butyl hydroperoxide. It is also capable of effectively eliminating superoxide and hydroxyl radical. The peroxidase loses activity in the presence of peroxide unless it is stabilized by ascorbate (Asc) or solutions such as aqueous humor or medium 199. While thiol but not disulfides inactivates the microPx-11, it is not inhibited in the presence of the rat lens which has a high GSH content. microPx-11 at concentrations 10 to 50 fold greater than are required to achieve good protective activity exhibits no toxicity based on cell viability, ATP levels and lens transparency after long-term incubations of alpha TN4-1 cells or cultured rat lens. The peroxidase is capable of protecting cultured rat lenses from photochemical stress where H2O2, O2.- and OH. are generated based on transparency, choline transport, epithelial cell viability and protein integrity as indicated by SDS-PAGE of the rat lens protein. In the absence of the peroxidase, extensive epithelial cell death and other degradative changes are observed. The DNA of alpha TN4-1 cells can also be protected from H2O2 induced single strand breaks by the microPx-11. The overall results suggest that a number of cytochrome C proteolytic degradation products are peroxidases which may be effective anti-cataract agents protecting the lens from oxidative stress.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Spector A,Ma W,Wang RR,Kleiman NJdoi
10.1006/exer.1997.0336subject
Has Abstractpub_date
1997-10-01 00:00:00pages
457-70issue
4eissn
0014-4835issn
1096-0007pii
S0014-4835(97)90336-5journal_volume
65pub_type
杂志文章abstract：:Prostaglandin E2 (PGE2) markedly reduces intraocular pressure (IOP) when applied topically and induces strong relaxation of pre-contracted isolated ciliary muscle through PGE2 receptor. Because the ciliary muscle relaxation reduces IOP by enhancing uveoscleral aqueous outflow, the ciliary muscle where the existence of...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0829
更新日期：2000-05-01 00:00:00
abstract：:A substantial change in the glycoconjugates of the rat interphotoreceptor matrix (IPM) has recently been shown to occur between post-natal day (P) 14 and P16 in rat retinas using lectin histochemistry. This suggests that the sialic acid content on the terminus of N-glycoside linked carbohydrate chains of the IPM incre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1013
更新日期：1993-01-01 00:00:00
abstract：:Retinal pigment epithelial cells (RPEs) are thought to be one of the main components of fibrous membrane observed in eyes with proliferative vitreo-retinopathy. We investigated the signalling mechanisms of TGF-beta2-dependent collagen gel contraction by RPEs. An in vitro type I collagen gel contraction assay was perfo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.09.024
更新日期：2006-05-01 00:00:00
abstract：:Autophagy is a lysosomal degradative process that is essential for cellular homeostasis and metabolic stress adaptation. Defective autophagy is involved in the pathogenesis of many diseases including granular corneal dystrophy type 2 (GCD2). GCD2 is an autosomal dominant disorder caused by substitution of histidine fo...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2015.09.008
更新日期：2016-03-01 00:00:00
abstract：:The aquaporins (AQPs) are a family of more than 10 homologous water transporting proteins expressed in many mammalian epithelia and endothelia. At least five AQPs are expressed in the eye: AQP0 (MIP) in lens fiber, AQP1 in cornea endothelium, ciliary and lens epithelia and trabecular meshwork, AQP3 in conjunctiva, AQP...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/s0014-4835(02)00303-2
更新日期：2003-02-01 00:00:00
abstract：PURPOSE:Cellular therapy with mesenchymal stem cells (MSC) is emerging as an effective option to treat optic neuropathies. In models of retinal degeneration, MSC injected in the vitreous body protects injured retinal ganglion cells and stimulate their regeneration, however the mechanism is still unknown. Considering th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108212
更新日期：2020-11-01 00:00:00
abstract：:The histopathological features of uveitis and retinal vasculitis in acute experimental allergic encephalomyelitis (EAE) were investigated using light and electron microscopy. Lewis rats were immunized by spinal cord homogenate, complete Freund's adjuvant and Bordetella pertussis. The eyes of rats with EAE exhibited va...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1024
更新日期：1993-02-01 00:00:00
abstract：:The effect of dietary supplementation with acetylsalicylic acid on the increased modification, and susceptibility to modification, of lens crystallins from the streptozocin diabetic rat, has been determined. This was done by the measurement of characteristic markers of protein post-translational oxidative modification...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1186
更新日期：1993-12-01 00:00:00
abstract：:A micropuncture technique involving the use of microcannulas with tip diameters less than 5 microns was used to measure the pressure in Schlemm's canal and in the meshwork at distances approximately 7 and 14 microns from the inner wall of Schlemm's canal. In one set of experiments where the spontaneous intraocular pre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90151-h
更新日期：1992-06-01 00:00:00
abstract：:There has been a drive to develop new cell based therapies to treat corneal blindness, one of the most common causes of blindness worldwide. Mechanical and physical cues are known to regulate the behavior of many cell types, however studies examining these effects on corneal epithelial cells have been limited in numbe...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2018.08.001
更新日期：2018-12-01 00:00:00
abstract：:The human lens crystallin gene CRYGC T5P is associated with Coppock-like cataract and has a phenotype of a dust-like opacity of the fetal lens nucleus and deep cortical region. Previous in vitro mutation studies indicate that the protein has changed conformation, solubility, and stability, which may make it susceptibl...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.08.021
更新日期：2008-12-01 00:00:00
abstract：:A HPLC method for determination of ascorbic acid and dehydroascorbic acid in plasma and aqueous and vitreous humors of rabbits is described. Values for total ascorbic acid concentration found in this study are in agreement with those of previous investigators. Endotoxin-induced ocular inflammation caused a decrease in...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(85)80019-1
更新日期：1985-09-01 00:00:00
abstract：:It has been suggested that human iris pigment epithelial (IPE) cells isolated from iridectomized tissue could be used as autologous cells for transplantation into the subretinal space in diseases with dysfunctional retinal pigment epithelium (RPE). RPE cells synthesize a number of cytokines and their receptors which a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0517
更新日期：1998-08-01 00:00:00
abstract：:Use has been made of the specific interactions between monoclonal antibodies and the alpha A or alpha B subunits of alpha-crystallin to devise methods for the purification of the intact protein or its subunits. alpha A and alpha B subunits were separated by affinity chromatography on an immobilized monoclonal antibody...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80007-1
更新日期：1988-04-01 00:00:00
abstract：:In ocular surface inflammatory diseases, such as dry eye disease, long-term symptom relief requires targeting the inflammation itself rather than treating only the surface-associated dryness with artificial tears. Therefore, we included an anti-inflammatory agent in an unpreserved liposome-based (LP) formulation used ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.11.010
更新日期：2017-01-01 00:00:00
abstract：:Corneal epithelial injury induces release of endogenous metabolites that are cannabinoid receptor 1 (CB1) and transient receptor potential vanilloid 1 (TRPV1) agonists. We determined the functional contributions by CB1 and TRPV1 activation to eliciting responses underlying wound healing in human corneal epithelial cel...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.06.022
更新日期：2010-09-01 00:00:00
abstract：:The causes of vitreous humor (VH) liquefaction remain unclear. Diabetes accelerates this process and other ocular diseases. The weakening of the blood-retina barrier observed with diabetes could enhance the rate of transfer of relatively small molecules such as glucose (Glu) and phospholipids (PLs) from the retina to ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108221
更新日期：2020-11-01 00:00:00
abstract：:The hyaluronan content during wound healing following excimer laser photoablation was investigated. Rabbit corneas were photoablated (Summit Omnimed, 193 nm, 5 mm diameter, 50 microns and 100 microns depth). Central optical zones of photoablated and contralateral corneas were removed 7, 30 and 90 days after surgery. C...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0212
更新日期：1997-03-01 00:00:00
abstract：:The purpose of this study was to determine the aqueous levels and pharmacokinetics of topical fluconazole 0.2% upon single and multiple drop applications. Forty-nine patients undergoing cataract surgery were given topical fluconazole 0.2%. They either received single drop or a loading dose of 1 drop per 5 min for 20 m...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0936
更新日期：2001-02-01 00:00:00
abstract：:Paired human lenses ranging from 2 to 96 yr old (n = 17) were harvested within 2 hr post-mortem and immersed in liquid nitrogen. Phosphorus containing metabolites from perchloric acid extracts were quantitated using phosphorus-31 nuclear magnetic resonance spectroscopy. Low-energy metabolites detected include the phos...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90016-8
更新日期：1991-06-01 00:00:00
abstract：:[3H]-retinol binding to cellular retinol-binding protein (CRBP) increased more than five-fold in developing chicken corneal epithelium at 14 days of incubation, the time when the initial increase in corneal transparency occurs in the developing embryo. In contrast, [3H]-retinoic acid binding to cellular retinoic acid-...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90026-x
更新日期：1983-11-01 00:00:00
abstract：:Axial-length and refraction measurements are presented for the lid-sutured rhesus monkey at 6-month intervals during the first 1.5 years of life. The experimental data are fitted to an exponential growth theory allowing the calculation of time constants, apparent time origins, and correlation coefficients for 10 eyes ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(86)90043-6
更新日期：1986-06-01 00:00:00
abstract：PURPOSE:This study examined the patterned treatment of corneal collagen cross-linking (CXL) for keratoconus to reduce the complications caused by ultraviolet (UV) irradiation. By modifying the method of UV irradiation during the cross-linking process, cross-linking with a special structure is achieved, and the cross-li...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2021.108450
更新日期：2021-01-23 00:00:00
abstract：:Several scleral cross-linking (SXL) methods were suggested to increase the biomechanical stiffness of scleral tissue and therefore, to inhibit axial eye elongation in progressive myopia. In addition to scleral cross-linking and biomechanical effects caused by riboflavin and light irradiation such a treatment might ind...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.07.005
更新日期：2015-10-01 00:00:00
abstract：:The purpose of the study was to determine histologic findings after successful laser trabeculoplasty in eyes with glaucoma. Previous studies have examined eyes in which laser did not lower intraocular pressure, or examined only the region of the laser burn itself. Nineteen autopsy eyes from 14 donors with either prima...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.07.009
更新日期：2007-10-01 00:00:00
abstract：:The effect of angiotensin II was studied in vitro on ring segments of bovine retinal resistance arteries (i.d. 126-271 microns) and posterior ciliary arteries (i.d. 207-1153 microns). Although the retinal resistance arteries were responsive to 5-hydroxytryptamine, prostaglandin F2 alpha, and changes in extracellular K...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90034-r
更新日期：1990-05-01 00:00:00
abstract：:The following lectins: Con A, WGA, sWGA, PNA, RCA and UEA were used to study developmental changes in the expression of glycoconjugates during chicken lens morphogenesis. Con A, WGA, sWGA binding epitopes were observed in the lens placode and vesicle. Once the fiber mass was formed, the glycoconjugates were mainly fou...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0047
更新日期：1996-04-01 00:00:00
abstract：:Corneal autofluorescence, as measured with a commercial scanning fluorophotometer (lambda(exc): 415-491 nm; lambda(em): 515-630 nm), is increased in patients with diabetes mellitus. However, such fluorophotometers register an average fluorescence signal over all corneal layers as a consequence of their limited axial r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0733
更新日期：1999-11-01 00:00:00
abstract：:This study presents a computational model of the growth of the normal human lens and the induction of spoke-like cortical cataract in the aging lens. The anterior lens is modelled as a 2-D disk with a circumferential germinative zone. Lens cortical fibre cells in the same generation cover the surface in three identica...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0883
更新日期：2000-09-01 00:00:00
abstract：:Incorporated in the luminal glycocalyx of vascular endothelia (EC) are negatively charged microdomains (anionic sites). These sites are considered functionally important (a) in their interaction with circulating blood constituents, and (b) as a determinant of vascular permeability. The molecular composition of these E...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80134-4
更新日期：1995-10-01 00:00:00
