Abstract：
:Müller cells (MC) represent a key element for the metabolic and functional regulation of the vertebrate retina. The aim of the present study was to test the feasibility of a new method for the in-vivo detection and quantification of extrafoveal MC in human retina. We developed a new approach to isolate and analyse extrafoveal MC in vivo, starting from structural optical coherence tomography data. Our pilot investigation was based on the optical properties of MC, which are known to not interfere with the light reaching the outer retinal structures. We reconstructed MC in the macular region of 18 healthy subjects and the quantitative analyses revealed ~42,000/9 mm2 cells detected. Furthermore, we included 2 patients affected by peripheral intraocular melanoma, with macular sparing, needing surgical enucleation. We used these two eyes to perform a qualitative comparison between our reconstructions and histological findings. Our study represents the first pilot investigation dedicated on the non-invasive isolation and quantification of MC, in-vivo, in human retina. Although we are aware that our study has several limitations, first of all related with the proper detection of foveal MC, because of the peculiar z-shape morphology, this approach may open new opportunities for the non-invasive in vivo analysis of MC, providing also potential useful perspectives in retinal diseases.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Arrigo A,Perra C,Aragona E,Giusto D,Doglioni C,Pierro L,Giordano Resti A,Bandello F,Battaglia Parodi Mdoi
10.1016/j.exer.2020.108183subject
Has Abstractpub_date
2020-10-01 00:00:00pages
108183eissn
0014-4835issn
1096-0007pii
S0014-4835(20)30441-3journal_volume
199pub_type
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