Abstract:
:The objective of this study were to establish a cell line derived from porcine hyalocytes and to investigate the regulation of hyaluronan (HA) synthesis in response to cytokines. After 50 passages of the cells derived from porcine vitreous tissue, a cell line was generated. The immortalized cells showed fibroblastic morphology. The cell doubling time was 56.9h. In the mRNA level, the cells expressed plate-derived growth factor (PDGF) alpha receptor, PDGF beta receptor, transforming growth factor-beta (TGF-beta) type I receptor, TGF-beta type II receptor, CD44, collagen type I, collagen type II, glial fibrillary acidic protein (GFAP), hyaluronan synthase (HAS) 2, HAS 3 and beta-actin. In the protein level, GFAP was expressed in this cell line. S-100 protein and cytokeratin were not detected. Stimulation with TGF-beta1 and/or PDGF-BB induced a marked increase in the expression level of HAS2 mRNA, and induced HA production. TGF-beta1 stimulated HAS2 expression through the signal transduction pathway including Smad 2,3,4. In summary, this report constitutes the first successful immortalization of porcine hyalocyte cells. The production of HA was induced from the generated porcine hyalocyte cell line under the stimulation of TGF-beta1 and/or PDGF-BB, which may be related to the pathogenesis of proliferative membrane formation in proliferative vitreo-retinal diseases.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Nishitsuka K,Kashiwagi Y,Tojo N,Kanno C,Takahashi Y,Yamamoto T,Heldin P,Yamashita Hdoi
10.1016/j.exer.2007.07.006subject
Has Abstractpub_date
2007-10-01 00:00:00pages
539-45issue
4eissn
0014-4835issn
1096-0007pii
S0014-4835(07)00194-7journal_volume
85pub_type
杂志文章abstract::In a previous investigation into the fate of fluorescently labelled antigen (Ag) injected into the anterior chamber (AC) of the rat eye, a large number of Ag+ cells were noted in the conventional and non-conventional aqueous humour outflow pathways together with the external limbus. The aim of this study was to invest...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.06.009
更新日期:2004-10-01 00:00:00
abstract::The purpose of this study was to investigate the influence of hyperoxia and hypercapnia on fundus pulsations in the macular and optic disc region and determine a potential autoregulative capacity of the choroid under these conditions. In a randomized cross-over study 100% O2 and a mixture of 5% CO2 with air was inhale...
journal_title:Experimental eye research
pub_type: 临床试验,杂志文章,随机对照试验
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更新日期:2007-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2015-12-01 00:00:00
abstract::The irritative response to Nd:YAG laser capsulotomy was studied in unanaesthetized rabbits. Posterior lens capsulotomy with a total energy of 100 mJ had no effect on the pupil size but increased the intraocular pressure by 5-10 mmHg and caused a breakdown of the blood-aqueous barrier. Anterior lens capsulotomy with a ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(86)80030-6
更新日期:1986-10-01 00:00:00
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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doi:10.1016/0014-4835(83)90026-x
更新日期:1983-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2014-09-01 00:00:00
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更新日期:2011-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90091-6
更新日期:1992-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0478
更新日期:1998-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90067-9
更新日期:1986-12-01 00:00:00
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journal_title:Experimental eye research
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doi:10.1016/s0014-4835(89)80044-2
更新日期:1989-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1083
更新日期:1994-07-01 00:00:00
abstract::It has long been assumed that unstimulated tears are more thoroughly equilibrated with epithelial secretions than stimulated tears, since they are in contact with tarsal, bulbar and corneal surfaces for longer. It was also believed from results with model solutions that soluble mucin is responsible for the observed su...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0600
更新日期:1999-02-01 00:00:00
abstract::Previous studies have shown that the inhibition of Rho kinase is involved in the regulation of outflow facility in the live rabbit eye and the enucleated porcine eye. However, it is unknown whether the Rho kinase inhibition will do the same in non-human primates. To determine if the Rho kinase inhibitor Y-27632 will r...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.09.002
更新日期:2005-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2010-12-01 00:00:00
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pub_type: 杂志文章
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journal_title:Experimental eye research
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更新日期:1983-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.07.019
更新日期:2007-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.09.017
更新日期:2010-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.02.006
更新日期:2009-06-15 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0234
更新日期:1997-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.02.009
更新日期:2016-04-01 00:00:00
abstract:PURPOSE:This microscopic study was undertaken to compare the melanocytes of peripheral iridectomy specimens from two eyes that had latanoprost-induced iris darkening (LIID) with iridectomies taken from the fellow untreated eyes. METHODS:The two patients in this study were the ones who underwent LIID in the latanoprost...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2003.08.009
更新日期:2003-12-01 00:00:00
abstract::An extraneous exon-skipping mRNA encodes an altered form of a light-absorbing opsin in human retina and pigment epithelium (RPE). The predicted protein variant differs from full-length RPE-retinal G protein-coupled receptor (RGR) by having an in-frame deletion of exon 6, which contains the entire sixth transmembrane d...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.11.013
更新日期:2006-07-01 00:00:00