Abstract：
:Collagen was isolated from bovine cornea and tested for reactivity towards platelets and IgG-coated polystyrene latex particles. The corneal collagen caused a dose- and temperature-dependent platelet aggregation in all human platelet-rich plasmas studied. As little as 0 . 31 micrograms of purified corneal collagen could trigger platelet activation. Human C1q, a subcomponent of the first complement component (C1), which shares extensive chemical-structural similarities with collagen, was able to inhibit the platelet aggregation provoked by corneal collagen. This blocking effect could be, however, overcome by increasing collagen doses. In a slide method or in aggregometry both corneal collagen and C1q agglutinated IgG-coated latex particles in a dose-dependent manner. Addition of such latex particles to platelet-rich plasmas or preincubation of the particles with collagen reduced or prevented the platelet action of collagen, suggesting that due to their reactivity with collagen immune complexes may play an inhibitory role in collagen-caused platelet aggregation. The results are discussed in relation to pathological events that lead to collagenolysis and also with respect to wound healing in the injured cornea.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Csako G,Suba EA,Herp Adoi
10.1016/0014-4835(83)90122-7subject
Has Abstractpub_date
1983-03-01 00:00:00pages
403-14issue
3eissn
0014-4835issn
1096-0007journal_volume
36pub_type
杂志文章abstract：:The polypeptides in portions of human lens epithelium from individuals of various ages were resolved by two-dimensional (2D) gel electrophoresis. The epithelium was divided into a central area of 12.5 mm2, a surrounding area of 50.2 mm2, and an area which was outside of this region. The proteins were solubilized in ur...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90064-l
更新日期：1991-05-01 00:00:00
abstract：:Transforming growth factor β (TGFβ) signaling is one of the most important signaling pathways regulating cell behavior in ocular tissues. Its functions are mainly linked to tissue fibrosis and inflammatory responses in ophthalmology. In epithelial cells, however, the growth inhibitory activity of TGFβ was reported in ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.03.014
更新日期：2019-05-01 00:00:00
abstract：:The presence of cartilage-matrix molecules in the embryonic chick eye was shown by means of measuring RNA expression and immunostaining studies. The molecules examined were proteoglycan core protein (PG-core), link protein (LP), cartilage-matrix protein (CMP) and type II collagen. The genes encoding these proteins are...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80061-7
更新日期：1988-05-01 00:00:00
abstract：:Elevated permeability of retinal endothelial cells (REC), as observed in diabetic retinopathy (DR), is induced by extended exposure to ≥25 ng/ml vascular endothelial growth factor A165 (VEGF165) for up to 3 d and this effect is more pronounced when equimolar amounts of basic fibroblast growth factor (bFGF) and insulin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.07.018
更新日期：2013-10-01 00:00:00
abstract：:The state of water and water fractions in normal and senile cataractous eye lenses was studied by the NMR method. Combining NMR with vacuum dehydration provided additional information on multifractional samples. A new mathematical procedure is presented which separates the characteristic parameters of the different fr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90104-5
更新日期：1983-05-01 00:00:00
abstract：:Corneal mycotic ulceration is predominantly due to Aspergillus and Fusarium solani infection in tropical countries. In this study, we examined the proteome profile of tear samples from A. flavus keratitis patients at various stages of infection. The proteome was profiled using 2D PAGE and the protein levels were quant...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107700
更新日期：2019-09-01 00:00:00
abstract：:The retinoschisin protein is encoded on the short arm of the X-chromosome by RS1, is expressed abundantly in photoreceptor inner segments and in bipolar cells, and is secreted as an octamer that maintains the structural integrity of the retina. Mutations in RS1 lead to X-linked retinoschisis (XLRS), a disease characte...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108344
更新日期：2021-01-01 00:00:00
abstract：BACKGROUND:The laser-induced choroidal neovascularization (CNV) mouse model, as the most classic animal model of age-related macular degeneration (AMD), has been widely used. We designed a hand-held mouse holder to optimize mouse fixation in the laser-induced CNV modelling process, which was inconvenient until now. Thi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108392
更新日期：2020-12-15 00:00:00
abstract：:Aim of present investigation was to study the effect of catechin and the combination of catechin and cetirizine in ovalbumin induced animal model of allergic conjunctivitis. Guinea pigs were divided into 5 groups: normal control, disease control, disease treated with catechin 100 mg/kg, disease treated with cetirizine...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.05.020
更新日期：2015-08-01 00:00:00
abstract：:Microaneurysms, acellular capillaries and pericyte ghosts are characteristic of diabetic retinopathy, but it is not clear what causes these lesions or whether they are causally related to each other. The distribution of microaneurysms, acellular capillaries and pericyte ghosts has been evaluated in two animal models o...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80069-7
更新日期：1995-05-01 00:00:00
abstract：:In this study, we examined calcium-mediated degradation of a neurofilament protein (NFP), and autolytic activation of calpain in Lewis rat optic nerve in vitro. After incubation with calcium, homogenized optic nerve samples were analysed by SDS-PAGE in association with ECL immunoblot techniques. 68 kD NFP, calpain, an...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0286
更新日期：1997-07-01 00:00:00
abstract：:To assess the ability of dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) to detect blood retinal barrier (BRB) damage in patients with diabetic macular edema (DME). DCE-MRI with 0.1 mmol Gd-DTPA was used to measure BRB permeability in 10 healthy and visually normal subjects and eight patients with DME, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.01.015
更新日期：2005-07-01 00:00:00
abstract：:The protein phosphatase 2B inhibitor, FK506, is an immunomodulatory polypeptide that has neuroprotective properties, the mechanisms of which have not been elucidated. A possible mechanism may be phosphorylation-mediated regulation of glutamate transporter activity. In the present study, we investigated the effect of F...
journal_title：Experimental eye research
pub_type： 信件
doi：10.1016/j.exer.2010.04.011
更新日期：2010-08-01 00:00:00
abstract：:In a previous investigation into the fate of fluorescently labelled antigen (Ag) injected into the anterior chamber (AC) of the rat eye, a large number of Ag+ cells were noted in the conventional and non-conventional aqueous humour outflow pathways together with the external limbus. The aim of this study was to invest...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.06.009
更新日期：2004-10-01 00:00:00
abstract：:Whole mounts of lens epithelia from rats fed on a galactose-rich diet for one, two, three or seven days were examined for morphological alterations. The meridional rows of epithelia from lenses of animals fed on galactose for seven days were found to be grossly disorganized or abnormally elongated. There was some indi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90183-0
更新日期：1984-06-01 00:00:00
abstract：:The purpose of this study was to examine the effects of myopia-inducing and myopia recovery conditions on the scleral biomechanics of enucleated eyes of young chicks. Enucleated eyes from 5-day old chicks, with fiducial markers attached at 5 locations on the external sclera, were placed in a custom-built chamber fille...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.07.004
更新日期：2014-10-01 00:00:00
abstract：:The identification of genes that modify pathological ocular phenotypes in mouse models may improve our understanding of disease mechanisms and lead to new treatment strategies. Here, we identify modifier loci affecting photoreceptor cell loss in homozygous Mfrp(rd6) mice, which exhibit a slowly progressive photorecept...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.10.020
更新日期：2014-01-01 00:00:00
abstract：:Ischemic preconditioning (IPC) protects the rat retina against the injury that ordinarily follows severe ischemia. We showed previously that release of adenosine and de novo protein synthesis were required for IPC protection. The mechanisms of IPC were studied in the rat retina by examining the signal transduction med...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0843
更新日期：2000-06-01 00:00:00
abstract：:Optic fissure closure defects result in uveal coloboma, a potentially blinding condition affecting between 0.5 and 2.6 per 10,000 births that may cause up to 10% of childhood blindness. Uveal coloboma is on a phenotypic continuum with microphthalmia (small eye) and anophthalmia (primordial/no ocular tissue), the so-ca...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2020.107940
更新日期：2020-04-01 00:00:00
abstract：:Intravitreal cidofovir has been shown to be a long acting and highly efficacious treatment for CMV retinitis; however decrease in IOP is an adverse effect. We wanted to determine the effect of cidofovir on intraocular pressure (IOP) in the guinea pig, and rabbit eye to develop an animal model of cidofovir induced ocul...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0273
更新日期：1997-05-01 00:00:00
abstract：PURPOSE:To assess the inter-ocular and inter-session reliability for a range of parameters derived from the photopic electroretinogram (ERG) in a group of normal non-human primates. METHODS:Inter-ocular differences for photopic ERGs were assessed in a group of normal anesthetized adult rhesus monkeys (Macaca mulatta, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.09.013
更新日期：2004-01-01 00:00:00
abstract：:Preretinal pH was measured in the avascular region of 19 rabbit retinas during alternating cycles of light and dark, using a miniature, needle-type pH electrode. In 14 experiments with the rabbits breathing room air, cyclic changes in pH, decreasing in the dark and increasing in the light, were observed. The transitio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90106-o
更新日期：1991-12-01 00:00:00
abstract：:The Purkinje cell degeneration (pcd) mutant mouse rapidly loses cerebellar Purkinje cells and about 50% of its retinal photoreceptor cells at between 3 and 5 weeks of age, and thereafter slowly loses the remaining photoreceptor cells during the first year of life. An ultrastructural study of the developing photorecept...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90019-o
更新日期：1992-05-01 00:00:00
abstract：:Experiments were conducted to evaluate the role played by photoreceptor cells in the accumulation of age pigment, or lipofuscin, in the retinal pigment epithelium (RPE). The age-related accumulation of RPE lipofuscin was compared between rats with hereditary photoreceptor degeneration (RDY) and congenic rats with norm...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(86)80023-9
更新日期：1986-10-01 00:00:00
abstract：:Fibroblast growth factors play a key role in regulating lens epithelial cell proliferation and differentiation via an anteroposterior gradient that exists between the aqueous and vitreous humours. FGF-2 is the most important for lens epithelial cell proliferation and differentiation. It has been proposed that the pres...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.12.004
更新日期：2014-03-01 00:00:00
abstract：:An experimental study was conducted on eight litters of newborn rats to evaluate the effects of supplemental oxygen administration on the retinal vasculature. The animals and their mothers were kept inside a pressure chamber and treated for the first 5 days of life. On the sixth day, they were removed and kept for fiv...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80179-3
更新日期：1987-03-01 00:00:00
abstract：:PAX6-related Aniridia is a sight-threatening disease involving progression of secondary glaucoma and aniridia related keratopathy (ARK). Change or loss of limbal epithelial progenitors causes epithelial surface defects. We analyzed the effect of PAX6 on mRNA expression changes with a two-step approach, as follows. Fir...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.10.002
更新日期：2019-02-01 00:00:00
abstract：:Myopia (short-sightedness) is a visual problem associated with excessive eye growth and vitreous chamber expansion. Within the eye serotonin (5-hydroxytryptamine, 5-HT) appears to have a variety of effects, it alters retinal amacrine cell processing, increases intraocular pressure, constricts ocular blood vessels, and...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.03.021
更新日期：2005-11-01 00:00:00
abstract：:Research with animal models of Pseudomonas aeruginosa keratitis has shown that use of a topical corticosteroid alone against an established infection can significantly increase the number of colonizing bacteria or worsen clinical disease. Moreover, retrospective analysis has suggested that corticosteroid use in humans...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.10.010
更新日期：2019-02-01 00:00:00
abstract：:Interphotoreceptor retinoid-binding protein (IRBP) greatly enhances the conversion of all- trans -retinol to 11- cis -retinal by the retinal pigment epithelium (RPE) and facilitates 11- cis -retinal release from the RPE. However, the mechanisms by which IRBP exerts these effects are not clear. Using a model system of ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0781
更新日期：2000-02-01 00:00:00
