Abstract:
:Iron accumulation in the retina is associated with the development of age-related macular degeneration (AMD). IV iron is a common method to treat iron deficiency anemia in adults, and its retinal manifestations have not hitherto been identified. To assess whether IV iron formulations can be retina-toxic, we generated a mouse model for iron-induced retinal damage. Male C57BL/6J mice were randomized into groups receiving IV iron-sucrose (+Fe) or 30% sucrose (-Fe). Iron levels in neurosensory retina (NSR), retinal pigment epithelium (RPE), and choroid were assessed using immunofluorescence, quantitative PCR, and the Perls' iron stain. Iron levels were most increased in the RPE and choroid while levels in the NSR did not differ significantly in +Fe mice compared to controls. Eyes from +Fe mice shared histological features with AMD, including Bruch's membrane (BrM) thickening with complement C3 deposition, as well as RPE hypertrophy and vacuolization. This focal degeneration correlated with areas of high choroidal iron levels. Ultrastructural analysis provided further detail of the RPE/photoreceptor outer segment vacuolization and Bruch's membrane thickening. Findings were correlated with a clinical case of a 43-year-old patient who developed numerous retinal drusen, the hallmark of AMD, within 11 months of IV iron therapy. Our results suggest that IV iron therapy may have the potential to induce or exacerbate a form of retinal degeneration. This retinal degeneration shares features with AMD, indicating the need for further study of AMD risk in patients receiving IV iron treatment.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Song D,Kanu LN,Li Y,Kelly KL,Bhuyan RK,Aleman T,Morgan JI,Dunaief JLdoi
10.1016/j.exer.2016.08.008subject
Has Abstractpub_date
2016-10-01 00:00:00pages
122-33eissn
0014-4835issn
1096-0007pii
S0014-4835(16)30219-6journal_volume
151pub_type
杂志文章abstract::The photopigment of avian pineal which mediates light sensitivity was sought via its chromophore. Chick pineal cells in primary cultures were incubated overnight in the dark with [3H]retinol. Reduction of Schiff's bases with cyanoborohydride prior to SDS-PAGE revealed radioactivity bound to a 30 kDa component in pinea...
journal_title:Experimental eye research
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doi:10.1016/s0014-4835(88)80042-3
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abstract::Eleven monoclonal antibodies against different segments of bovine rhodopsin were used with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal photoreceptor cells for visualization by transmission electron microscopy. Antibodies against the C-terminus, F1-F2 loop and N-terminus of rho...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90017-5
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.09.018
更新日期:2009-01-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80076-3
更新日期:1987-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80009-0
更新日期:1995-06-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80210-6
更新日期:1992-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.07.018
更新日期:2013-10-01 00:00:00
abstract::Iron is an essential biometal in the aqueous humor, the principal source of nutrients for the avascular cornea and the lens. Here, we explored whether the ciliary body (CB), the source of aqueous humor, transports iron, and if the prion protein (PrPC) facilitates this process as in the outer retina. Using a combinatio...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.05.031
更新日期:2018-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1077
更新日期:2001-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1015
更新日期:1993-01-01 00:00:00
abstract::Mammalian lens development involves cell-cell and cell-ECM interactions. As integrins are a major family of cell adhesion molecules, we examined the expression patterns of several integrin subunits (alpha3A, alpha3B, alpha6A, alpha6B, beta1 and beta4) during rat lens development. RT-PCR, in situ hybridisation, immunof...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.02.005
更新日期:2005-09-01 00:00:00
abstract:PURPOSE:Cellular therapy with mesenchymal stem cells (MSC) is emerging as an effective option to treat optic neuropathies. In models of retinal degeneration, MSC injected in the vitreous body protects injured retinal ganglion cells and stimulate their regeneration, however the mechanism is still unknown. Considering th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108212
更新日期:2020-11-01 00:00:00
abstract::Osteopontin (OPN) is elevated during the progression of experimental autoimmune uveoretinitis (EAU) in C57BL/6 (B6) mice. Furthermore, EAU symptoms are ameliorated in OPN knockout mice or in B6 mice treated with anti-OPN antibody (M5). Recently, OPN has been shown to promote the Th1 response not only in the extracellu...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.09.008
更新日期:2010-01-01 00:00:00
abstract::A newly-developed fibre-optic sensor (wavelength of 670 nm) was used to measure the index gradient in 16 human lenses ranging in age from 38 to 78 years. Two of these lenses had nuclear cataract. The refractive index, calculated from the proportion of reflected light at the sensor/sample interface, appeared to increas...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80097-1
更新日期:1995-04-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80002-7
更新日期:1987-02-01 00:00:00
abstract::Between 5 and 70% of genetic disease is caused by in-frame nonsense mutations, which introduce a premature termination codon (PTC) within the disease-causing gene. Consequently, during translation, non-functional or gain-of-function truncated proteins of pathological significance, are formed. Approximately 50% of all ...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2017.01.001
更新日期:2017-02-01 00:00:00
abstract::An extraneous exon-skipping mRNA encodes an altered form of a light-absorbing opsin in human retina and pigment epithelium (RPE). The predicted protein variant differs from full-length RPE-retinal G protein-coupled receptor (RGR) by having an in-frame deletion of exon 6, which contains the entire sixth transmembrane d...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.11.013
更新日期:2006-07-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2010.10.013
更新日期:2010-12-01 00:00:00
abstract::The aquaporins (AQPs) are a family of more than 10 homologous water transporting proteins expressed in many mammalian epithelia and endothelia. At least five AQPs are expressed in the eye: AQP0 (MIP) in lens fiber, AQP1 in cornea endothelium, ciliary and lens epithelia and trabecular meshwork, AQP3 in conjunctiva, AQP...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/s0014-4835(02)00303-2
更新日期:2003-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0273
更新日期:1997-05-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.03.020
更新日期:2009-09-01 00:00:00
abstract::The expression and intracellular localization of the estrogen receptor (ER) subtypes, ERalpha and ERbeta were examined in cultured human lens epithelial cells (HLE-B3). ERalpha and ERbeta mRNA expression was evaluated by coupled reverse transcription-polymerase chain reaction (RT-PCR) and Southern blot analysis. Subce...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2003.09.027
更新日期:2004-04-01 00:00:00
abstract::We examined the expression of mGlu2/3 metabotropic glutamate receptors in the retina of the goldfish Carassius auratus. mGlu2/3 receptors were expressed in all retinal layers internal to the photoreceptor layer, particularly in the outer and inner nuclear layers. Although the goldfish brain is able to tolerate prolong...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.11.008
更新日期:2007-03-01 00:00:00
abstract::There is increasing evidence that the macular pigment carotenoids, lutein and zeaxanthin, may play an important role in the prevention of age-related macular degeneration, cataract, and other blinding disorders. Although it is well known that the retina and lens are enriched in these carotenoids, relatively little is ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0954
更新日期:2001-03-01 00:00:00
abstract::The present study was designed to investigate the effect of topical erythropoietin on the healing process of induced necrotizing scleritis and to evaluate the ocular side effects of this treatment modality in a rabbit model. Necrotizing scleritis was induced in 8 New Zealand albino rabbits. The animals were then rando...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107898
更新日期:2020-01-01 00:00:00
abstract::The purpose of this study was to evaluate the composition of ghost vessels and the newly identified intravitreal structures type 1 and 2 (IVS-1 and 2) observed in the rabbit vitreous body. Rabbit eyes (n = 10, 0.5- approximately 36 months of age) were fixed and embedded in plastic. Post-embedding immuno transmission e...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0865
更新日期:2000-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107837
更新日期:2019-12-01 00:00:00
abstract::Crystallin-bound non-tryptophan fluorescence and protein cross-linking were studied in chronic galactosemia. Fluorescence (excitation/emission--370/440 nm) was significantly higher in galactosemic rats compared to controls (P less than 0.001). Accumulation of fluorescence was significantly reduced both in water solubl...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90153-l
更新日期:1990-10-01 00:00:00
abstract::The polypeptides in portions of human lens epithelium from individuals of various ages were resolved by two-dimensional (2D) gel electrophoresis. The epithelium was divided into a central area of 12.5 mm2, a surrounding area of 50.2 mm2, and an area which was outside of this region. The proteins were solubilized in ur...
journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1991-05-01 00:00:00