Abstract:
:Pseudoexfoliation syndrome (PEX) may lead to the development of pseudoexfoliative glaucoma (PEG), a potential cause of irreversible blindness, if left untreated. This type of glaucoma often presents with much higher intraocular pressure (IOP) values than observed in primary open angle glaucoma, and patients are often unaware of their condition. Therefore, early diagnosis is of utmost importance in PEX and PEG. Unfortunately, no valid objective biomarkers are available that can be used for this purpose. The excessive synthesis and deposition of elastic microfibrillar pseudoexfoliation material is observed in the pathophysiology of PEX, therefore, growth factors may play roles in this pathology. Thus, in this study, we sought to determine the roles of phenotypes and genotypes of connective tissue growth factor (CTGF) as objective biomarkers for early diagnosis of PEX and PEG. Thus, we investigated possible associations involving tear and aqueous humor CTGF concentrations and four single nucleotide polymorphisms (SNPs) of the CTGF gene in PEX and PEG. The study was designed as a 2-year case-control study in the Turkish population. Study population was composed of 214 patients with PEG, 214 patients with PEX, and 214 age-matched controls for CTGF SNP analysis. Tear fluid study group consisted of 78 patients with PEG, 77 patients with PEX, and 78 controls. Aqueous humor analysis included 8 patients with PEG, 17 patients with PEX, and 23 controls. Tear fluid was collected using Schirmer strips, and aqueous humor samples were taken during cataract surgery. CTGF concentration was determined by ELISA, and total protein concentration was determined by Bradford assay in tear and aqueous humor samples. PCR followed by restriction fragment length polymorphism analysis was used for genotyping of rs6918698 G/C and rs9399005 C/T, while real-time PCR was used for rs9402373 C/G and rs12526196 T/C. Intraocular pressure, visual field score, mean deviation, and pattern standard deviation parameters were also evaluated. CTGF concentration in tear fluid was significantly higher in PEG patients compared with controls (P = 0.001), while it was lower in PEX patients. Similarly, total protein concentration in tear fluid was significantly increased in PEG patients relative to PEX patients (P = 0.026) and controls (P = 0.004). CTGF concentration in aqueous humor did not differ markedly between the groups, whereas total protein was significantly higher in the PEG group compared with the PEX group (P = 0.012) and controls (P = 0.003). Receiver operating characteristic analysis revealed that total protein in aqueous humor was a robust classifier for evaluating the presence of PEG against controls (Area under the curve = 0.897, P = 0.001). The genotypes of the studied SNPs were not significantly correlated with CTGF concentration in aqueous humor or tear fluid, and did not exhibit significant association with PEG or PEX. In conclusion, this was the first study to investigate tear fluid CTGF concentration in PEX and PEG, which came out not to be a good classifier for PEG or PEX. Total protein level in tear fluid and CTGF SNPs also did not predict PEG or PEX status successfully.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Can Demirdöğen B,Koçan Akçin C,Özge G,Mumcuoğlu Tdoi
10.1016/j.exer.2019.107837subject
Has Abstractpub_date
2019-12-01 00:00:00pages
107837eissn
0014-4835issn
1096-0007pii
S0014-4835(19)30428-2journal_volume
189pub_type
杂志文章abstract::Transforming growth factor-beta (TGF-β) is one of the main epithelial-mesenchymal transition (EMT)-inducing factors. In general, TGF-β-induced EMT promotes cell migration and invasion. TGF-β also acts as a potent regulator of pericellular proteolysis by regulating the expression and secretion of plasminogen activators...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.06.020
更新日期:2013-10-01 00:00:00
abstract::We investigated the inhibitory action of a synthetic peptidyl hydroxamate inhibitor of matrix metalloproteinase (MMP), Galardin (GM6001), on collagen degradation by rabbit corneal stromal fibroblasts (keratocytes) cultured three-dimensionally in the type I collagen gel with medium containing interleukin 1alpha (IL-1al...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0637
更新日期:1999-05-01 00:00:00
abstract::An optimal system for monitoring in vivo corneal wound healing is inexpensive, has utility for wounding and imaging, and is able to provide previews before photography. We outline such an imaging system that takes advantage of a consumer digital camera and an LED-based light source for fluorescein excitation. Using FV...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.01.021
更新日期:2007-06-01 00:00:00
abstract::Na,K-ATPase was detected in crude microsomes of guinea-pig retinal layers, cornea and lens by immunoblotting using antibodies generated against purified kidney Na,K-ATPase. The antiserum cross-reacted with the alpha catalytic subunit of the enzyme from the eye tissues but not the beta glycoprotein subunit, suggesting ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90136-8
更新日期:1985-05-01 00:00:00
abstract::In response to injury, the adult mammalian retina shows signs of structural remodeling, possibly in an attempt to preserve or regain some of its functional neural connections. In order to study the mechanisms involved in injury-induced plasticity, we have studied changes in growth associated protein 43 (GAP-43) after ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.01.006
更新日期:2007-05-01 00:00:00
abstract::The hyaluronan content during wound healing following excimer laser photoablation was investigated. Rabbit corneas were photoablated (Summit Omnimed, 193 nm, 5 mm diameter, 50 microns and 100 microns depth). Central optical zones of photoablated and contralateral corneas were removed 7, 30 and 90 days after surgery. C...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0212
更新日期:1997-03-01 00:00:00
abstract::Cynomolgus (Macaca fascicularis) monkeys from three families, which showed symptoms of early onset macular degeneration was studied. Two proteins, albumin and glyceraldehyde 3-phosphate dehydrogenase, were found to have markedly altered concentrations in whole retina of the monkeys with early onset macular degeneratio...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0026
更新日期:1996-03-01 00:00:00
abstract::miRs play critical roles in oxidative stress-related retinopathy pathogenesis. miR-365 was identified in a previously constructed library from glyoxal-treated rat Müller cell. This report explores epigenetic alterations in Müller cells under oxidative stress to develop a novel therapeutic strategy. To examine the miR-...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.11.006
更新日期:2018-03-01 00:00:00
abstract::In retinal degenerative disease (RD), the diminished light signal from dying photoreceptors has been considered the sole cause of visual impairment. Recent studies show a 10-fold increase in spontaneous activity in the RD network, challenging this paradigm. This aberrant activity forms a new barrier for the light sign...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.05.013
更新日期:2016-09-01 00:00:00
abstract::The transcription factor Prox1 acts in rodent retinogenesis, at least in promoting cell cycle withdrawal and horizontal cell production. In the mature retina, this protein is detected at the inner nuclear layer of all vertebrate groups. We have made a neurochemical characterisation of Prox1(+) cell types in two differ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.10.015
更新日期:2010-02-01 00:00:00
abstract::Research in animal models have shown that exposing the eye to positive or negative spectacle lenses can lead to predictable changes in eye growth. Recent research indicates that brief periods (1-2 h) of monocular defocus results in small, but significant changes in axial length and choroidal thickness of human subject...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.03.019
更新日期:2019-05-01 00:00:00
abstract::Two-dimensional 1H NMR spectroscopic studies are presented on bovine gamma S- and gamma B-crystallin. In gamma S-crystallin, the four N-terminal residues have great flexibility compared with the rest of the molecule and assume a random coil conformation. NMR spectroscopy and electrospray mass spectrometry show that th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1099
更新日期:1994-08-01 00:00:00
abstract::To apply nuclear magnetic resonance (NMR) spectroscopy to study the penetration of dexamethasone phosphate into the aqueous humour from rabbit following topical administration. After topical administration of 0.1%, 1.0% and 10% dexamethasone phosphate solutions, respectively, samples of aqueous humour were aspirated, ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0429
更新日期:1998-03-01 00:00:00
abstract::The CLN2 form of neuronal ceroid lipofuscinosis is an autosomal recessively inherited lysosomal storage disease that is characterized by progressive vision loss culminating in blindness, cognitive and motor decline, neurodegeneration, and premature death. CLN2 disease results from mutations in the gene that encodes th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.02.012
更新日期:2015-05-01 00:00:00
abstract::Following the identification of potent, orally active inhibitors of aldose reductase, the next challenge comes in demonstrating clinical utility in the treatment of diabetic complications. It is not feasible to conduct pivotal studies in all indications within the same trial and neuropathy evolved as the lead indicati...
journal_title:Experimental eye research
pub_type: 临床试验,杂志文章,评审
doi:10.1016/0014-4835(90)90103-2
更新日期:1990-06-01 00:00:00
abstract::Intravitreal cidofovir has been shown to be a long acting and highly efficacious treatment for CMV retinitis; however decrease in IOP is an adverse effect. We wanted to determine the effect of cidofovir on intraocular pressure (IOP) in the guinea pig, and rabbit eye to develop an animal model of cidofovir induced ocul...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0273
更新日期:1997-05-01 00:00:00
abstract::Exposure to sulfur mustard (SM) may result in severe ocular injuries. While some of the eyes show a clinical resolution of the injury (defined as clinically non-impaired), part of the eyes develop irreversible late ocular pathologies (defined as clinically impaired) that may lead to corneal blindness. Understanding th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.04.011
更新日期:2019-07-01 00:00:00
abstract::Several lines of evidence support the existence of a renin-angiotensin system (RAS) in the retina that is separated from the blood stream by the retinal pigment epithelium (RPE). Under physiological conditions, increased activity of intraretinal RAS regulates neuronal activity of the retina but patho-physiologically p...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107828
更新日期:2019-12-01 00:00:00
abstract::The benzopyran BP (3,4-dihydro-6-hydroxy-7-methoxy-2,2-dimethyl-1(2H)-benzopyran) is a free radical scavenger that is structurally similar to alpha-tocopherol and has provided neuro-protection in a number of disease models where oxidative stress is a causative factor. A novel derivative of BP with improved lipid solub...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.04.007
更新日期:2011-07-01 00:00:00
abstract::Reactive astrocytes in glaucomatous optic nerve changes are characterized by an increased expression of alphaB-crystallin and transforming growth factor-beta (TGF-beta). In the pathogenesis of glaucomatous optic nerve damage, ischemia/reperfusion injury may play an important role. The goal of the present study was to ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.12.008
更新日期:2007-04-01 00:00:00
abstract::Our previous studies in bovine eyes demonstrated that the structural correlate to the increase in outflow facility after either Rho-kinase inhibitor Y-27632 (Y27) treatment or washout appeared to be separation between the juxtacanalicular tissue (JCT) and inner wall (IW) of the aqueous plexus, the bovine equivalent of...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.05.012
更新日期:2011-10-01 00:00:00
abstract::Glaucoma is defined as a progressive optic neuropathy and is characterized by an irreversible loss of retinal ganglion cells. The main risk factor to develop glaucoma is an increased intraocular pressure (IOP). During the course of glaucoma structural changes in the optic nerve head (ONH) take place which lead to the ...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2015.08.014
更新日期:2016-01-01 00:00:00
abstract::Calf lens alpha-crystallin was isolated and the lysine residues were extensively modified with a variety of chemical agents. The effect of these modifications on elastase inhibitor activity, apparent molecular size, antibody reactivity and solubility were determined. The addition of either a methyl group or a threose ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1015
更新日期:1993-01-01 00:00:00
abstract::The lacrimal functional unit (LFU) is defined by the 2007 International Dry Eye WorkShop as 'an integrated system comprising the lacrimal glands, ocular surface (cornea, conjunctiva and meibomian glands) and lids, and the sensory and motor nerves that connect them'. The LFU maintains a healthy ocular surface primarily...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2008.09.002
更新日期:2009-05-01 00:00:00
abstract::The purpose of this study was to investigate the effects of abnormal-cannabidiol (abn-cbd), a non-psychoactive cannabinoid agonist, on aqueous humor outflow via the trabecular meshwork (TM) of porcine eye, and to examine the involvement of a non-CB1/CB2 cannabinoid receptor and the p42/44 mitogen-activated protein kin...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.05.001
更新日期:2012-07-01 00:00:00
abstract::Longitudinal (T1) magnetic relaxation times for the major phosphorus-containing metabolites present in the bovine and rabbit lens under organ culture conditions and in the bovine and rabbit globe have been determined. Significant differences in T1 for the major phosphorus metabolites in each case are observed, as well...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90045-9
更新日期:1984-10-01 00:00:00
abstract::Early-onset Fuchs endothelial corneal dystrophy (FECD) has been associated with nonsynonymous mutations in collagen VIII α2 (COL8A2), a key extracellular matrix (ECM) protein in Descemet's membrane (DM). Two knock-in strains of mice have been generated to each express a mutant COL8A2 protein (Col8a2L450W/L450W and Col...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.11.021
更新日期:2019-03-01 00:00:00
abstract::All aqueous humor draining through the conventional outflow pathway must cross the endothelium of Schlemm's canal (SC), likely by passing through micron-sized transendothelial pores. SC pores are non-uniformly distributed along the inner wall endothelium, but it is unclear how the distribution of pores relates to the ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.11.008
更新日期:2015-01-01 00:00:00
abstract::This study was conducted to compare the effects of two anti-VEGF-A drugs, Ranibizumab and Aflibercept, on the expression and secretion of VEGFs family members, and on their influence in proliferation and migration of endothelial cells (HECV) in vitro. HECV cells were exposed 24 h (T1), 4 days (T2) and 6 days (T3) to R...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.12.014
更新日期:2016-05-01 00:00:00
abstract::Changes in activities of ascorbate free radical (AFR) reductase (NADH:AFR oxidoreductase) and H2O2-dependent NADH oxidation were correlated with levels of insoluble protein in senile cataractous human lenses. The H2O2-dependent NADH oxidation activity was measured to reflect the content of free glutathione. AFR reduct...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90128-h
更新日期:1990-06-01 00:00:00