Abstract:
:This study was conducted to compare the effects of two anti-VEGF-A drugs, Ranibizumab and Aflibercept, on the expression and secretion of VEGFs family members, and on their influence in proliferation and migration of endothelial cells (HECV) in vitro. HECV cells were exposed 24 h (T1), 4 days (T2) and 6 days (T3) to Ranibizumab or Aflibercept at pharmacodynamically relevant concentrations (Ranibizumab: 12.5 μg/ml and 125 μg/ml; Aflibercept: 50 μg/ml and 500 μg/ml). Cell viability and then expression and secretion of VEGF-A, VEGF-B, VEGF-C and PlGF were evaluated respectively by Real Time-PCR and ELISA. Intracellular signaling activated by VEGF-A and VEGF-C was investigated evaluating phosphorylation of VEGFR2. Influence in would healing was evaluated through scratch assay. In general no differences were observed among the tested concentrations of anti-vegf drugs. Ranibizumab and Aflibercept did not affect HECV cell viability in all experimental times. At T1, Ranibizumab decreased mRNA levels of VEGF-A, induced VEGF-C secretion, abrogated phosphorylation of VEGFR2 stimulated by VEGF-A, and impaired ability of HECV cells to repair wound healing. Aflibercept decreased mRNA levels of VEGF-A, -B and PlGF; slightly increased basal level of phVEGFR2, and completely abrogated phosphorylation stimulated by VEGF-A and VEGF-C. No effects on secretion of VEGF-B and on would healing were observed after exposure to Aflibercept. Prolonged exposure to anti-VEGFs decreased expression and secretion of VEGF-A and VEGF-B, up-regulated VEGF-C mRNA levels and its secretion, and increased basal phosphorylation of VEGFR2. Acute treatment with Ranibizumab or Aflibercept evoked different responses on endothelial cells, however these differences were lost after prolonged exposure. Scratch test results suggest that treatment with Ranibizumab may be more effective than Aflibercept in reducing angiogenic potential of endothelial cells in vitro.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Puddu A,Sanguineti R,Traverso CE,Viviani GL,Nicolò Mdoi
10.1016/j.exer.2015.12.014subject
Has Abstractpub_date
2016-05-01 00:00:00pages
128-36eissn
0014-4835issn
1096-0007pii
S0014-4835(15)30099-3journal_volume
146pub_type
杂志文章abstract::The lacrimal functional unit (LFU) is defined by the 2007 International Dry Eye WorkShop as 'an integrated system comprising the lacrimal glands, ocular surface (cornea, conjunctiva and meibomian glands) and lids, and the sensory and motor nerves that connect them'. The LFU maintains a healthy ocular surface primarily...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2008.09.002
更新日期:2009-05-01 00:00:00
abstract::Endocannabinoid system involves in neuroprotective effects on the central neural system. The cannabinoid receptor 1 (CB1R) is widely expressed in the mouse retina. However, the role of cannabinoid receptors in the retina remains unclear. In this work, we established a photoreceptor degeneration mouse model via N-methy...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.11.001
更新日期:2018-02-01 00:00:00
abstract::Human retinal pigment epithelial cells in culture demonstrate adenylate cyclase activity. It is membrane-bound and modulated by GTP regulatory proteins. It is effectively activated only by beta-adrenergic agonists (L-isoproterenol greater than or equal to L-epinephrine greater than L-norepinephrine) and some prostagla...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80158-6
更新日期:1987-04-01 00:00:00
abstract::The following lectins: Con A, WGA, sWGA, PNA, RCA and UEA were used to study developmental changes in the expression of glycoconjugates during chicken lens morphogenesis. Con A, WGA, sWGA binding epitopes were observed in the lens placode and vesicle. Once the fiber mass was formed, the glycoconjugates were mainly fou...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0047
更新日期:1996-04-01 00:00:00
abstract::Exposure to sulfur mustard (SM) may result in severe ocular injuries. While some of the eyes show a clinical resolution of the injury (defined as clinically non-impaired), part of the eyes develop irreversible late ocular pathologies (defined as clinically impaired) that may lead to corneal blindness. Understanding th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.04.011
更新日期:2019-07-01 00:00:00
abstract::In order to determine whether neuronal cell death in the retina accompanies senescence, retinas from pigmented ACI rats of various ages were analysed for changes in dimensions, cell densities, DNA content, and rhodopsin levels. Between the ages of 4 and 30 months, there were significant declines in the densities of nu...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90022-9
更新日期:1986-04-01 00:00:00
abstract::Glycoconjugate mucin secretion from conjunctival goblet cells is tightly regulated by nerves and specialized pro-resolving mediators (SPMs) to maintain ocular surface health. Here we investigated the actions of the SPM resolvin E1 (RvE1) on cultured rat conjunctival goblet cell glycoconjugate secretion and intracellul...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.04.015
更新日期:2018-08-01 00:00:00
abstract::TGFβ2 induces extracellular matrix (ECM) remodeling and alters the cytoskeleton by both the canonical Smad and non-canonical signaling pathways. TGFβ2 regulates the expression of ECM proteins in trabecular meshwork (TM) cells, increases intraocular pressure (IOP) in an ex vivo perfusion organ culture model, and induce...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2013.10.017
更新日期:2013-11-01 00:00:00
abstract::Prolyl oligopeptidase (EC 3.4.21.26) has been purified 26,000-fold from bovine lens tissue by anion-exchange chromatography, gel filtration and isoelectric focussing, with an overall yield of 11%. The purified enzyme exhibited an isoelectric point of 4.8, a pH optimum of 7.5 and a molecular mass of 72 kDa under both n...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1086
更新日期:1994-07-01 00:00:00
abstract::Two-dimensional 1H NMR spectroscopic studies are presented on bovine gamma S- and gamma B-crystallin. In gamma S-crystallin, the four N-terminal residues have great flexibility compared with the rest of the molecule and assume a random coil conformation. NMR spectroscopy and electrospray mass spectrometry show that th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1099
更新日期:1994-08-01 00:00:00
abstract::The spontaneously hypertensive rats (SHR) show a lower intraocular pressure--despite high arterial blood pressure--than the normotensive Wistar Kyoto rats (NR). In SHR the quantitative evaluation of resin casts and flat-mount preparations of the blood vessels of the ciliary body and choroid showed a significant dilati...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90004-1
更新日期:1985-02-01 00:00:00
abstract::A dark reaction is known to occur in retinal extracts of the gecko (Gekko gekko), in which the natural 11-cis-chromophore of the 521-pigment is apparently replaced by adding 9-cis-retinal to form the 9-cis-photopigment. With chloride-deficient extracts the reaction involves some 70% of the 521-pigment. Anions like nit...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90074-2
更新日期:1989-07-01 00:00:00
abstract::RPE65-related Leber's congenital amaurosis (LCA) is a rod-cone dystrophy whose clinical outcome is mainly attributed to the loss of rod photoreceptors followed by cone degeneration. Pathogenesis in Rpe65(-/-) mice is characterized by a slow and progressive degeneration of rods dependent on the constitutive activation ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.12.019
更新日期:2012-03-01 00:00:00
abstract::The irritative response to Nd:YAG laser capsulotomy was studied in unanaesthetized rabbits. Posterior lens capsulotomy with a total energy of 100 mJ had no effect on the pupil size but increased the intraocular pressure by 5-10 mmHg and caused a breakdown of the blood-aqueous barrier. Anterior lens capsulotomy with a ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(86)80030-6
更新日期:1986-10-01 00:00:00
abstract::The effects of temperature on rod outer segment (ROS) length and membrane shedding were studied in a cyprinodont fish, Fundulus zebrinus. After 30 days in 14L/10D cyclic light and 17 degrees C, ROS length averaged 41.2 microns. Fish were then exposed to 7, 17 or 27 degrees C for 10 and 25 days before being sampled 5 h...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80036-3
更新日期:1995-08-01 00:00:00
abstract::Identification of novel agents to eradicate the residual lens cell population following cataract surgery provides one mode of preventing PCO formation. The present study investigated the biological mechanism of As(2)O(3) cytotoxicity in a human lens cell line and capsular bag system. FHL 124 cell survival was assessed...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.08.018
更新日期:2007-12-01 00:00:00
abstract::Developing canine retina, fixed in Bouin's or Tellyesnizky's acetic alcoholic formalin, was examined with a battery of biotinylated lectins. ConA and WGA consistently bound to all retinal layers including the tapetum cellulosum, retinal pigment epithelium, inner and outer segments, plexiform layers and ganglion cells....
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90150-s
更新日期:1990-10-01 00:00:00
abstract::Reactive nitrogen species, in particular, peroxynitrite (ONOO(-)) have been proposed to play an important role in the pathogenesis of endotoxin-induced uveitis (EIU). Tyrosine nitration by ONOO(-) has been shown in other model systems to inhibit the activity of the superoxide anion quenching enyzme, manganese superoxi...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2002.1141
更新日期:2002-04-01 00:00:00
abstract::We have previously shown that corticotropin-releasing hormone plays an important proinflammatory role in the induction of experimental autoimmune uveoretinitis. In this study, we examined the role of apoptosis in the destruction of the retina during experimental autoimmune uveoretinitis, and the role of corticotropin-...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.0992
更新日期:2001-06-01 00:00:00
abstract::The advance of optical coherence tomography (OCT) enables a detailed examination of the human retina in-vivo for clinical routine and experimental eye research. Only few investigations to date captured human foveal morphology in a large subject group on the basis of a detailed analysis employing mathematical models. H...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.05.013
更新日期:2016-07-01 00:00:00
abstract::Keratoconus is a debilitating corneal thinning disease that principally develops in the second and third decades of life. Our group previously developed a novel approach to studying keratoconus, based on the observation that there is a gradient of damage across the keratoconic cone. We identified a number of cellular ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(03)00148-9
更新日期:2003-10-01 00:00:00
abstract::Understanding how tissues and organs acquire and maintain an appropriate size and shape remains one of the most challenging areas in developmental biology. The eye lens represents an excellent system to provide insights into regulatory mechanisms because in addition to its relative simplicity in cellular composition (...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2016.04.009
更新日期:2017-03-01 00:00:00
abstract::The wound-healing response is critical to the outcome of refractive surgery and studying wound healing contributes to an understanding of the pathophysiology of other corneal injuries. Animal models allow research to be conducted with sufficient samples and under controlled parameters. We studied the hen to determine ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.02.017
更新日期:2006-10-01 00:00:00
abstract::We have studied the growth of neurites from single retinal ganglion cells isolated from adult goldfish and maintained under various primary cell culture conditions. In 10% Leibovitz's L-15 medium at 23 degrees C, these ganglion cells remained viable for up to 10 days and generated extensive fields of neurites. We foun...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(91)90257-f
更新日期:1991-02-01 00:00:00
abstract::The calcium-binding regulatory protein, calmodulin, was immunohistologically examined in feline rod outer segments. The peroxidase antiperoxidase technique was used for the immunohistological analysis and protein A-gold, to localize calmodulin electron-microscopically. Calmodulin was found to be located in the distal ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80178-1
更新日期:1987-03-01 00:00:00
abstract::Ocular rigidity (OR) is thought to play a role in the pathogenesis of glaucoma, but the lack of reliable non-invasive measurements has been a major technical challenge. We recently developed a clinical method using optical coherence tomography time-lapse imaging and automated choroidal segmentation to measure the puls...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107831
更新日期:2020-01-01 00:00:00
abstract::The carbohydrate epitope Gal alpha 1-3Gal-R (alpha-galactosyl epitope), which is detectable by its binding with Bandeiraea simplicifolia-IB4 lectin, was found in glycosphingolipids (GSLs), both neutral and acidic (gangliosides), from lens tissues of non-primate mammals, but not in those of human senile cataracts and O...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1151
更新日期:1994-12-01 00:00:00
abstract::Immunocytochemistry with a monoclonal antibody against the 28-kDa calcium-binding protein (28-kDa CaBP) was used to study horizontal cells in both congenic control (RCS-rdy+) and dystrophic (RCS) 3-month-old rat retina. We found that horizontal cells in the RCS-rdy+ rat retinas were distributed evenly from the centre ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1109
更新日期:1993-08-01 00:00:00
abstract::The objective of this study was to determine the cellular localization of P2Y(2) receptor gene expression in rabbit and primate ocular tissues using the technique of non-isotopic in situ hybridization. Fresh frozen whole eye from a New Zealand White rabbit and whole eye and eyelid from a rhesus macaque were cut into 5...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(03)00068-x
更新日期:2003-07-01 00:00:00
abstract::The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl-D-glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional gluc...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(83)90011-8
更新日期:1983-02-01 00:00:00