Abstract:
:Endocannabinoid system involves in neuroprotective effects on the central neural system. The cannabinoid receptor 1 (CB1R) is widely expressed in the mouse retina. However, the role of cannabinoid receptors in the retina remains unclear. In this work, we established a photoreceptor degeneration mouse model via N-methyl-N-nitrosourea (MNU) administration to identify the neuroprotective effects of cannabinoid receptors. The MNU-induced retinal degeneration behaves similarly to that in the human retinitis pigmentosa (RP). Administration of the CB1R antagonist SR141716A distinctly recovered the photoreceptor loss, decreased glial reactivity and reduced abnormal vascular complexes in an MNU-induced mouse model. The BC dendrites were shrunk in the MNU-treated retina with eliminated ON-BCs responses and partially diminished OFF-BCs responses in patch-clamp recordings. In the MNU + SR1 group, both the function and structure of ON-BCs recovered. Taken together, our study showed that the inhibition of CB1R can effectively prevent MNU-induced retinal degeneration, suggesting a potential therapeutic effect of the CB1R antagonist SR1 in retinal degeneration diseases.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Chen Y,Luo X,Liu S,Shen Ydoi
10.1016/j.exer.2017.11.001subject
Has Abstractpub_date
2018-02-01 00:00:00pages
145-151eissn
0014-4835issn
1096-0007pii
S0014-4835(17)30508-0journal_volume
167pub_type
杂志文章abstract::The sarcoplasmic-endoplasmic reticulum calcium ATPase (SERCA) is a key intracellular calcium transporter, which regulates cellular calcium concentration [Ca2+] by transporting Ca2+ ions from the cytosol into the endoplasmic reticulum. SERCA-mediated Ca2+ sequestration controls proper folding of newly synthesized prote...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.04.007
更新日期:2005-12-01 00:00:00
abstract::Retinitis Pigmentosa (RP) is an inherited disorder that may lead to blindness. In the rhodopsin S334ter-line-3 rat model of RP, the death of rods induces spatial rearrangement of cones into regular ring mosaics. Using this model, we discovered that the ring mosaics are restored to a homogeneous distribution upon appli...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.08.005
更新日期:2015-11-01 00:00:00
abstract:PURPOSE:Cellular therapy with mesenchymal stem cells (MSC) is emerging as an effective option to treat optic neuropathies. In models of retinal degeneration, MSC injected in the vitreous body protects injured retinal ganglion cells and stimulate their regeneration, however the mechanism is still unknown. Considering th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108212
更新日期:2020-11-01 00:00:00
abstract::To apply nuclear magnetic resonance (NMR) spectroscopy to study the penetration of dexamethasone phosphate into the aqueous humour from rabbit following topical administration. After topical administration of 0.1%, 1.0% and 10% dexamethasone phosphate solutions, respectively, samples of aqueous humour were aspirated, ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0429
更新日期:1998-03-01 00:00:00
abstract::The chelating agent Na2EDTA was perfused through the anterior chambers of monkeys eyes in an attempt to find a concentration that would cause a rise in outflow facility without harming the cornea or anterior uvea. Na2EDTA (0.5 mM) proved effective in increasing the facility within 60 min. There were marked effects on ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80002-7
更新日期:1987-02-01 00:00:00
abstract::Incorporated in the luminal glycocalyx of vascular endothelia (EC) are negatively charged microdomains (anionic sites). These sites are considered functionally important (a) in their interaction with circulating blood constituents, and (b) as a determinant of vascular permeability. The molecular composition of these E...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80134-4
更新日期:1995-10-01 00:00:00
abstract::By methods of subtraction-hybridization of lambda ZAP cDNA libraries, prepared from 4-week-old rat lens epithelial cells (capsule) and lens fiber cells (decapsulated lens), we have isolated a specific cDNA clone whose target mRNA is about 600 b long. Northern blot hybridization analysis data showed that the target mRN...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80009-0
更新日期:1995-06-01 00:00:00
abstract::The photopigment of avian pineal which mediates light sensitivity was sought via its chromophore. Chick pineal cells in primary cultures were incubated overnight in the dark with [3H]retinol. Reduction of Schiff's bases with cyanoborohydride prior to SDS-PAGE revealed radioactivity bound to a 30 kDa component in pinea...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(88)80042-3
更新日期:1988-06-01 00:00:00
abstract::Owing to a rapidly aging population, vision impairment caused by age-related cataract has become very common. Age-related cataract has also become one of the principal causes of blindness, and apoptosis of lens epithelial cells contributes to non-congenital cataract development. Previous studies have reported that mic...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.04.018
更新日期:2016-06-01 00:00:00
abstract::Transport of circulating sulfur amino acids (SAA) into the lens epithelium and de novo glutathione (GSH) synthesis were studied in the perfused guinea-pig eye. Plasma-to-aqueous transfer of SAA was in their intact form (> or = 98%) and comparable with sucrose (an extracellular marker) within 30 min. The unidirectional...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0260
更新日期:1997-04-01 00:00:00
abstract::Functional atrophy and accompanying lymphocytic infiltration and destruction of the lacrimal gland (LG) are characteristics of Sjögren's Syndrome (SjS). The male NOD mouse is an experimental model for the autoimmune exocrinopathy that develops in the LG of SjS patients. Acinar cells in LG of male NOD mice aged 3-4 mon...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.03.020
更新日期:2009-09-01 00:00:00
abstract::The purpose of this study was to investigate the influence of hyperoxia and hypercapnia on fundus pulsations in the macular and optic disc region and determine a potential autoregulative capacity of the choroid under these conditions. In a randomized cross-over study 100% O2 and a mixture of 5% CO2 with air was inhale...
journal_title:Experimental eye research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1016/s0014-4835(05)80019-3
更新日期:1995-12-01 00:00:00
abstract::Aquaporin-0 (AQP0), the major integral membrane protein in lens fiber cells, becomes highly modified with increasing age. The functional consequences of these modifications are being revealed, and the next step is to determine how these modifications affect the ocular lens, which is directly related to their abundance...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.10.007
更新日期:2011-12-01 00:00:00
abstract::The spontaneously hypertensive rats (SHR) show a lower intraocular pressure--despite high arterial blood pressure--than the normotensive Wistar Kyoto rats (NR). In SHR the quantitative evaluation of resin casts and flat-mount preparations of the blood vessels of the ciliary body and choroid showed a significant dilati...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90004-1
更新日期:1985-02-01 00:00:00
abstract::The aim of the study was to evaluate the antiproliferative and cytotoxic properties of triamcinolone acetonide (TA) on human retinal pigment epithelium cells (ARPE19) and the role of epicellular crystalline deposits. Monolayer cultures of ARPE19 cells were used. Purified or unpurified crystalline TA suspension (0.01-1...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.02.012
更新日期:2006-09-01 00:00:00
abstract::Myocilin is a 55-57kDa secreted glycoprotein and member of the olfactomedin family, which is mutated in some forms of primary open-angle glaucoma. To assess the effects of elevated amounts of myocilin on aqueous humor outflow dynamics in an in vivo system, transgenic betaB1-crystallin-MYOC mice have been developed tha...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.06.006
更新日期:2008-09-01 00:00:00
abstract::Recently, a unique distribution, namely a reduction of macular pigment optical density (MPOD) within the central retina with a surrounding ring-like structure of preserved MPOD at about 6 degrees eccentricity was suggested to be a common finding in macular telangiectasia (MacTel) type 2. In order to quantify this redu...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2009.02.006
更新日期:2009-06-15 00:00:00
abstract::Neovascular age-related macular degeneration (AMD) is a leading cause of vision loss worldwide. Although intravitreal injection of anti-VEGF antibodies and VEGF Trap have significant clinical benefits, the complications of intravitreal injection, drug resistance and patient compliance still need to be concerned. In th...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.12.009
更新日期:2018-03-01 00:00:00
abstract::Fiber cells of the ocular lens are arranged in a series of concentric shells. New growth shells are added continuously to the lens surface and, as a consequence, the preexisting shells are buried. To focus light, the refractive index of the lens cytoplasm must exceed that of the surrounding aqueous and vitreous humors...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2016.03.009
更新日期:2017-03-01 00:00:00
abstract::Na,K-ATPase was detected in crude microsomes of guinea-pig retinal layers, cornea and lens by immunoblotting using antibodies generated against purified kidney Na,K-ATPase. The antiserum cross-reacted with the alpha catalytic subunit of the enzyme from the eye tissues but not the beta glycoprotein subunit, suggesting ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90136-8
更新日期:1985-05-01 00:00:00
abstract::Previous studies have shown that corneal endothelial cells contain mRNA and protein of various growth factors. However, the role of these endogenous growth factors in corneal endothelial wound healing is not fully elucidated. In the present study, we investigated the role of endogenous factors and several growth facto...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2000.0918
更新日期:2000-12-01 00:00:00
abstract::The genetically encoded green fluorescent protein-based calcium sensor, GCaMP, has been used to detect calcium transients and report neuronal activity. We evaluated the specificity of GCaMP3 expression to retinal ganglion cells (RGCs) of the transgenic Thy1-GCaMP3 mouse line in healthy control animals and in those aft...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108297
更新日期:2021-01-01 00:00:00
abstract::Hyaluronan (HA) distribution in the posterior eye wall from the vitreous through the sclera, with special consideration to localization in the retina and interphotoreceptor matrix (IPM), was evaluated in human, bovine, guinea pig, dog, rat and mouse tissues using a specific probe for HA (bHABC, biotinylated hyaluronan...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0422
更新日期:1998-02-01 00:00:00
abstract::Identification of novel agents to eradicate the residual lens cell population following cataract surgery provides one mode of preventing PCO formation. The present study investigated the biological mechanism of As(2)O(3) cytotoxicity in a human lens cell line and capsular bag system. FHL 124 cell survival was assessed...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.08.018
更新日期:2007-12-01 00:00:00
abstract::The lacrimal glands of male NOD mice exhibit many of the features of the human lacrimal gland in patients afflicted with the autoimmune disease, Sjögren's syndrome, including loss of secretory functions and lymphocytic infiltration into the lacrimal gland. To elucidate the early changes in the secretory pathway associ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.04.019
更新日期:2006-01-01 00:00:00
abstract::The aim of this study was to optimize non-viral gene transfer conditions and investigate the effect of fibroblast growth factor-1 (FGF-1) gene transfer on human corneal endothelial cell (HCEC) proliferation. Five non-viral vectors (Lipofectin, DMRIE-C, DAC-30, Effectene, FuGene6) were used to transfect HCEC with plasm...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.08.024
更新日期:2005-01-01 00:00:00
abstract::Myopia is one of the leading ocular disorders causing visual impairment worldwide, with the prevalence increasing rapidly. It's a significant global public health concern in 21st century. Myopia, particularly high myopia, often exhibits apparent familial aggregation, and multiple evidences have shown that genetic fact...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2019.107778
更新日期:2019-11-01 00:00:00
abstract::Prolyl oligopeptidase (EC 3.4.21.26) has been purified 26,000-fold from bovine lens tissue by anion-exchange chromatography, gel filtration and isoelectric focussing, with an overall yield of 11%. The purified enzyme exhibited an isoelectric point of 4.8, a pH optimum of 7.5 and a molecular mass of 72 kDa under both n...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1086
更新日期:1994-07-01 00:00:00
abstract::Mammalian lens development involves cell-cell and cell-ECM interactions. As integrins are a major family of cell adhesion molecules, we examined the expression patterns of several integrin subunits (alpha3A, alpha3B, alpha6A, alpha6B, beta1 and beta4) during rat lens development. RT-PCR, in situ hybridisation, immunof...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.02.005
更新日期:2005-09-01 00:00:00
abstract::The purpose of this study was to compare younger and older mice after chronic intraocular pressure (IOP) elevation lasting up to 4 days with respect to mitochondrial density, structure, and movement, as well as axonal integrity, in an ex vivo explant model. We studied 2 transgenic mouse strains, both on a C57BL/6J bac...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2018.04.001
更新日期:2018-07-01 00:00:00