MicroRNA let-7b induces lens epithelial cell apoptosis by targeting leucine-rich repeat containing G protein-coupled receptor 4 (Lgr4) in age-related cataract.


:Owing to a rapidly aging population, vision impairment caused by age-related cataract has become very common. Age-related cataract has also become one of the principal causes of blindness, and apoptosis of lens epithelial cells contributes to non-congenital cataract development. Previous studies have reported that microRNA let-7b (let-7b) is upregulated in cataractous lens epithelial cells, and the expression level of let-7b is positively associated with N, C and P cataract scores. However, the role of let-7b in the development of age-related cataract remains unclear. Here, we observed that the expression level of let-7b in the anterior lens capsules of age-related cataract was significantly higher than that in the normal anterior lens capsules. We performed ultraviolet (UV) irradiation to induce lens epithelial cell apoptosis. The results showed that the expression level of let-7b in lens epithelial cells which were treated by UV irradiation was significantly higher than that in the control, and let-7b promoted UV irradiation-induced apoptosis. Furthermore, we showed that leucine-rich repeat containing G protein-coupled receptor 4 (Lgr4) was a direct target of let-7b, and let-7b modulated lens epithelial cell apoptosis by directly targeting Lgr4. These findings will offer new insights into our understanding of the molecular mechanisms underlying the pathogenesis of cataract.


Exp Eye Res


Dong Y,Zheng Y,Xiao J,Zhu C,Zhao M




Has Abstract


2016-06-01 00:00:00












  • The role of astrocytes in optic nerve head fibrosis in glaucoma.

    abstract::Glaucoma is defined as a progressive optic neuropathy and is characterized by an irreversible loss of retinal ganglion cells. The main risk factor to develop glaucoma is an increased intraocular pressure (IOP). During the course of glaucoma structural changes in the optic nerve head (ONH) take place which lead to the ...

    journal_title:Experimental eye research

    pub_type: 杂志文章,评审


    authors: Schneider M,Fuchshofer R

    更新日期:2016-01-01 00:00:00

  • Role of tyrosine-sulfated proteins in retinal structure and function.

    abstract::The extracellular matrix (ECM) plays a significant role in cellular and retinal health. The study of retinal tyrosine-sulfated proteins is an important first step toward understanding the role of ECM in retinal health and diseases. These secreted proteins are members of the retinal ECM. Tyrosine sulfation was shown to...

    journal_title:Experimental eye research

    pub_type: 杂志文章,评审


    authors: Kanan Y,Al-Ubaidi MR

    更新日期:2015-04-01 00:00:00

  • Analysis of foveal characteristics and their asymmetries in the normal population.

    abstract::The advance of optical coherence tomography (OCT) enables a detailed examination of the human retina in-vivo for clinical routine and experimental eye research. Only few investigations to date captured human foveal morphology in a large subject group on the basis of a detailed analysis employing mathematical models. H...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Scheibe P,Zocher MT,Francke M,Rauscher FG

    更新日期:2016-07-01 00:00:00

  • A 1H NMR spectroscopic comparison of gamma S- and gamma B-crystallins.

    abstract::Two-dimensional 1H NMR spectroscopic studies are presented on bovine gamma S- and gamma B-crystallin. In gamma S-crystallin, the four N-terminal residues have great flexibility compared with the rest of the molecule and assume a random coil conformation. NMR spectroscopy and electrospray mass spectrometry show that th...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Cooper PG,Carver JA,Aquilina JA,Ralston GB,Truscott RJ

    更新日期:1994-08-01 00:00:00

  • Retinal ganglion cell survival in development: mechanisms of retinal growth hormone action.

    abstract::Several variants of growth hormone (GH) are found in the retina and vitreous of the chick embryo, where they appear to act as cell survival factors, having neuroprotective effects on retinal ganglion cells (RGCs). Here, we investigate the molecular mechanisms of the anti-apoptotic effect of GH in cultured RGCs. GH tre...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Sanders EJ,Parker E,Harvey S

    更新日期:2006-11-01 00:00:00

  • Localization of thrombospondin-1 and myofibroblasts during corneal wound repair.

    abstract::Thrombospondin-1 (TSP-1) is a multifunctional matrix protein that has recently been examined in various wound processes, primarily for its ability to activate the latent complex of transforming growth factor-beta (TGF-β). TGF-β has been shown to play a major role in stimulating mesenchymal cells to synthesize extracel...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Matsuba M,Hutcheon AE,Zieske JD

    更新日期:2011-10-01 00:00:00

  • G-quartet oligonucleotide mediated delivery of proteins into photoreceptors and retinal pigment epithelium via intravitreal injection.

    abstract::There is currently no available method to efficiently deliver proteins across the plasma membrane of photoreceptor or retinal pigment epithelium (RPE) cells in vivo. Thus, current clinical application of recombinant proteins in ophthalmology is limited to the use of proteins that perform their biological function extr...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Leaderer D,Cashman SM,Kumar-Singh R

    更新日期:2016-04-01 00:00:00

  • Neuroprotective effect of transcorneal electrical stimulation on ischemic damage in the rat retina.

    abstract::Some previous studies have showed that transcorneal electrical stimulation (TES) could protect retinal neurons in certain rodent models. However, it is not yet clear whether TES could also definitely protect retinal neurons against ischemic insults. In the present study, we hypothesized that TES had such a neuroprotec...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Wang X,Mo X,Li D,Wang Y,Fang Y,Rong X,Miao H,Shou T

    更新日期:2011-11-01 00:00:00

  • The role of dopamine in eye growth responses to color and luminance flicker in chicks.

    abstract::Stroboscopic luminance flicker has been found to prevent the increase in eye growth normally associated with form deprivation through the release of retinal dopamine. In this study, we sought to investigate whether dopamine plays a role in the decreased growth observed with 2 Hz sine-wave luminance flicker and increas...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Chuang KK,Rucker FJ

    更新日期:2019-12-01 00:00:00

  • Biomechanical assessment in models of glaucomatous optic neuropathy.

    abstract::The biomechanical environment within the eye is of interest in both the regulation of intraocular pressure and the loss of retinal ganglion cell axons in glaucomatous optic neuropathy. Unfortunately, this environment is complex and difficult to determine. Here we provide a brief introduction to basic concepts of mecha...

    journal_title:Experimental eye research

    pub_type: 杂志文章,评审


    authors: Nguyen TD,Ethier CR

    更新日期:2015-12-01 00:00:00

  • The presence of keratin 5 as an IgG Fc binding protein in human corneal epithelium.

    abstract::Rabbit immunoglobulin G (IgG) binds specifically to the human corneal epithelium. We investigated this phenomenon to identify the binding material in the tissue. Sections of human cornea were immunostained with negative-control grade rabbit IgG, F(ab')(2) (the antigen recognition fragment of IgG yielded by pepsin dige...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Endo K,Kawasaki S,Nakamura T,Kinoshita S

    更新日期:2004-06-01 00:00:00

  • Angiotensin II does not contract bovine retinal resistance arteries in vitro.

    abstract::The effect of angiotensin II was studied in vitro on ring segments of bovine retinal resistance arteries (i.d. 126-271 microns) and posterior ciliary arteries (i.d. 207-1153 microns). Although the retinal resistance arteries were responsive to 5-hydroxytryptamine, prostaglandin F2 alpha, and changes in extracellular K...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Nyborg NC,Nielsen PJ,Prieto D,Benedito S

    更新日期:1990-05-01 00:00:00

  • Fabrication of corneal epithelial cell sheets maintaining colony-forming cells without feeder cells by oxygen-controlled method.

    abstract::The use of murine 3T3 feeder cells needs to be avoided when fabricating corneal epithelial cell sheets for use in treating ocular surface diseases. However, the expression level of the epithelial stem/progenitor cell marker, p63, is down-regulated in feeder-free culture systems. In this study, in order to fabricate co...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Nakajima R,Takeda S

    更新日期:2014-01-01 00:00:00

  • Relaxation of porcine retinal arterioles exposed to hypercholesterolemia in vivo is modified by hepatic LDL-receptor deficiency and diabetes mellitus.

    abstract::Metabolic disturbances in diabetes mellitus include changes in the type and concentration of lipids in the blood plasma which may contribute to the development of diabetic retinopathy. This disease is characterized by changes in retinal blood flow secondary to changes in the tone of retinal arterioles which is regulat...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Bek T,Al-Mashhadi RH,Misfeldt M,Riis-Vestergaard MJ,Bentzon JF,Pedersen SM

    更新日期:2013-10-01 00:00:00

  • Choroidal endothelial cells transmigrate across the retinal pigment epithelium but do not proliferate in response to soluble vascular endothelial growth factor.

    abstract::The purpose of this study was to investigate the effects of soluble VEGF on human choroidal endothelial cell (CEC) transmigration across an RPE monolayer as it relates to choroidal neovascularization in AMD. In coculture assays, ARPE-19 (ARPE) was plated on the undersides of Transwell inserts having 0.4 microm pores. ...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Geisen P,McColm JR,Hartnett ME

    更新日期:2006-04-01 00:00:00

  • Neutralization of IL-23 depresses experimental ocular neovascularization.

    abstract::Interleukin-23 (IL-23) is a heterodimeric cytokine that consists of p19, a novel subunit, and p40, which is shared by IL-12. IL-23 has been demonstrated to play an important role in autoimmunity and tumor growth. However, the role of IL-23 in ocular neovascularization (NV) diseases remains unclear. In this study, we e...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Cai Y,Tan W,Shen X,Zhu Y,Gao Y,Sui A,Lu Q,Zhong Y,Xie B

    更新日期:2016-05-01 00:00:00

  • Increases in collagen type IV and laminin in galactose-induced retinal capillary basement membrane thickening--prevention by an aldose reductase inhibitor.

    abstract::Biochemical alterations in the composition of retinal capillary basement membrane components were investigated in galactosemic rats, an animal model that develops basement membrane lesions comparable to those of diabetic retinopathy. Normotensive Wistar-Kyoto rats fed a 30% galactose diet for 9 months developed signif...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Das A,Frank RN,Zhang NL,Samadani E

    更新日期:1990-03-01 00:00:00

  • Transport of circulating reduced glutathione at the basolateral side of the anterior lens epithelium: physiologic importance and manipulations.

    abstract::Transport of circulating reduced glutathione (GSH) was studied at the basolateral side of the lens epithelium by using an in situ vascular eye perfusion technique in guinea-pigs with rapid sampling to ensure detecting initial uptakes. The unidirectional transport rates of [35S]-GSH (4 nM) from plasma and aqueous into ...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Mackic JB,Jinagouda S,McComb JG,Weiss MH,Kannan R,Kaplowitz N,Zlokovic BV

    更新日期:1996-01-01 00:00:00

  • A superfamily of NADPH-dependent reductases in eukaryotes and prokaryotes.

    abstract::Aldose reductase (AR) is implicated in some of the disabling complications of diabetes, including neuropathy, retinopathy and cataracts. Our studies are aimed at further clarifying the role of AR in diabetes and facilitating the design of new classes of potent, specific AR inhibitors by gaining an understanding of the...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Carper DA,Wistow G,Nishimura C,Graham C,Watanabe K,Fujii Y,Hayashi H,Hayaishi O

    更新日期:1989-09-01 00:00:00

  • Corneal stroma PDGF blockade and myofibroblast development.

    abstract::Myofibroblast development and haze generation in the corneal stroma is mediated by cytokines, including transforming growth factor-beta (TGF-beta), and possibly other cytokines. This study examined the effects of stromal PDGF-beta blockade on the development of myofibroblasts in response to -9.0 diopter photorefractiv...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Kaur H,Chaurasia SS,de Medeiros FW,Agrawal V,Salomao MQ,Singh N,Ambati BK,Wilson SE

    更新日期:2009-05-01 00:00:00

  • Horizontal cells of the normal and dystrophic rat retina: a wholemount study using immunolabelling for the 28-kDa calcium-binding protein.

    abstract::Immunocytochemistry with a monoclonal antibody against the 28-kDa calcium-binding protein (28-kDa CaBP) was used to study horizontal cells in both congenic control (RCS-rdy+) and dystrophic (RCS) 3-month-old rat retina. We found that horizontal cells in the RCS-rdy+ rat retinas were distributed evenly from the centre ...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Chu Y,Humphrey MF,Constable IJ

    更新日期:1993-08-01 00:00:00

  • Simvastatin induces caspase-dependent apoptosis and activates P53 in OCM-1 cells.

    abstract::Simvastatin is a cholesterol-lowering drug which exhibits numerous pleiotropic effects including anti-cancer activity. Yet, the anti-cancer effects in choroidal melanoma remain poorly characterized. Therefore, in this study, we investigated the effects of simvastatin on OCM-1 cells growth, apoptosis and cycle. Simvast...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Wang Y,Xu SL,Wu YZ,Zhao MS,Xu WJ,Yang HY,Li YX

    更新日期:2013-08-01 00:00:00

  • Plasminogen activator inhibitor-1 (PAI-1) stimulates human corneal epithelial cell adhesion and migration in vitro.

    abstract::In addition to its role as an inhibitor of urokinase plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1) is hypothesized to regulate epithelial cell adhesion and migration. We have previously reported that PAI-1 may be an important regulatory factor of the uPA system in cornea. The purpose of this s...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Wang Z,Sosne G,Kurpakus-Wheater M

    更新日期:2005-01-01 00:00:00

  • Role and regulation of CXC-chemokines in acute experimental keratitis.

    abstract::The aim of this study was to elucidate the expression of chemokines, their role and regulation in bacterial corneal infection using three bacterial strains (Pseudomonas. aeruginosa- invasive, cytotoxic and contact lens induced acute red eye strains) which have been shown to produce three distinct patterns of corneal d...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Xue ML,Thakur A,Willcox MD,Zhu H,Lloyd AR,Wakefield D

    更新日期:2003-02-01 00:00:00

  • Neuroprotective effect of Myo/Nog cells in the stressed retina.

    abstract::Myo/Nog cells are essential for eye development in the chick embryo and respond to injury in adult tissues. These cells express mRNA for the skeletal muscle specific transcription factor MyoD, the bone morphogenetic protein (BMP) inhibitor Noggin and the cell surface protein recognized by the G8 monoclonal antibody (m...

    journal_title:Experimental eye research

    pub_type: 杂志文章,评审


    authors: Bravo-Nuevo A,Brandli AA,Gerhart J,Nichols J,Pitts M,Sutera CK,Assali S,Scheinfeld V,Prendergast GC,Stone J,George-Weinstein M

    更新日期:2016-05-01 00:00:00

  • Metabolic memory and diabetic retinopathy: role of inflammatory mediators in retinal pericytes.

    abstract::Diabetic retinopathy shares many characteristics features of a low grade chronic inflammatory disease. Its progression resists arrest when good metabolic control is re-established after a period of poor metabolic control, suggesting a 'metabolic memory' phenomenon. The aim of this study is to investigate the effect of...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Kowluru RA,Zhong Q,Kanwar M

    更新日期:2010-05-01 00:00:00

  • Status of caveolin-1 in various membrane domains of the bovine lens.

    abstract::Recent studies of the distribution and relative concentration of caveolin-1 in fractions of bovine lens epithelial and fiber cells have led to the novel concept that caveolin-1 may largely exist as a peripheral membrane protein in some cells. Caveolin-1 is typically viewed as a scaffolding protein for caveolae in plas...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Cenedella RJ,Sexton PS,Brako L,Lo WK,Jacob RF

    更新日期:2007-10-01 00:00:00

  • L-NAME protects against acute light damage in albino rats, but not against retinal degeneration in P23H and S334ter transgenic rats.

    abstract::Two previous studies have shown that N(G)-nitro-L-arginine methyl ester (L-NAME), an inhibitor of neuronal nitric oxide synthase, protects retinas of albino rats and mice from damaging levels of light. The aims of the present study were two-fold: (1) to confirm the protective effect of L-NAME on wild type albino rats ...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Káldi I,Dittmar M,Pierce P,Anderson RE

    更新日期:2003-04-01 00:00:00

  • Complex carbohydrates at the ocular surface of the mouse: an ultrastructural and cytochemical analysis.

    abstract::Scanning electron microscopy (SEM) of mouse cornea and conjunctiva fixed with picric acid-paraformaldehyde-glutaraldehyde (PA-P-G) mixture revealed a thin layer of amorphous material covering the microvilli of the corneal surface cells. At the transmission electron microscopic (TEM) level, this layer of material stain...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Wells PA,Hazlett LD

    更新日期:1984-07-01 00:00:00

  • Cytoarchitectural changes in lens epithelium of galactose-fed rats.

    abstract::Whole mounts of lens epithelia from rats fed on a galactose-rich diet for one, two, three or seven days were examined for morphological alterations. The meridional rows of epithelia from lenses of animals fed on galactose for seven days were found to be grossly disorganized or abnormally elongated. There was some indi...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Gona O

    更新日期:1984-06-01 00:00:00