Abstract:
:The distribution of collagen degradation and synthesis (turnover) between the central and peripheral areas of clear corneal allografts was determined, following transplantation of 6-mm [3H]proline-labelled corneal buttons from extensively pre-labelled, young rabbits into 13 nonradioactive mature rabbits. Control, contralateral 6-mm corneal buttons of each radioactive pair were frozen at -70 degrees C after being trephined into a central 3-mm button and a peripheral ring of cornea. The grafts were removed 10-100 days after the operation and trephined similarly to the paired controls. The central and peripheral areas of the graft and control corneal buttons were analysed separately for total hydroxyproline and radioactivity. A significant degradation of old collagen (as seen by the loss of total radioactivity) occurred early in both the central (19.4 +/- 6.1%) and peripheral (12.3 +/- 7.5%) areas of the grafted corneas, compared to the corresponding areas of the contralateral controls. A significant increase in new, non-radioactive collagen occurred in both the central (22.0 +/- 7.6%) and peripheral areas (30.6 +/- 6.1%); however, a significant net increase in total collagen occurred only in the peripheral area (18.3 +/- 11.4). Loss of original (old) collagen was significantly greater in the central area than in the peripheral area; whereas, the increase in new collagen was significantly greater in the peripheral area than the central area, accounting for a significant increase in total collagen. There was no correlation in collagen turnover with time after surgery. Collagen turnover occurs acutely throughout the clear corneal graft and thereafter is stable.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Lass JH,Baker DS,Klein LRdoi
10.1016/s0014-4835(87)80076-3subject
Has Abstractpub_date
1987-07-01 00:00:00pages
39-43issue
1eissn
0014-4835issn
1096-0007journal_volume
45pub_type
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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journal_title:Experimental eye research
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更新日期:2016-07-01 00:00:00
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