Abstract:
:A method for the isolation and purification of human ocular mucin from the brief saline extract of human ocular mucus is reported. Initial purification of ocular mucin was achieved by sequential chromatography of the saline-soluble mucus extract from an individual donor's mucus pool on columns of Sephadex G-50 and Sepharose CL-4B. A portion of such mucin isolate was subjected to quantitative analysis of the O-seryl (threonyl)-N-acetylgalactosaminyl linkage, characteristic of mucins, by alkaline beta-elimination and tritiated borohydride reduction. Following Bio-Gel P-2 filtration, the mucin isolate whose cleaved oligosaccharides contained tritiated galactosaminitol greater than 0.5 microCi mg-1, a value that represents at least 64% of that observed for bovine and ovine submaxillary reference mucins, was considered to be mucin-rich. These isolates were subjected to further purification on Sephacryl S-500 and DEAE-Trisacryl M column chromatographies. The purified mucin had a minimum molecular weight of 120 kDa. It consisted of 25-30% protein and 54-55% carbohydrate. Its amino acid and carbohydrate compositions are characteristic of a mucin structure. The purity of the mucin was verified by SDS-gradient PAGE. Upon isoelectric focusing, polydispersity/microheterogeneity were exhibited in the pI range 5.0-6.6.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Chao CC,Butala SM,Herp Adoi
10.1016/0014-4835(88)90002-4subject
Has Abstractpub_date
1988-08-01 00:00:00pages
185-96issue
2eissn
0014-4835issn
1096-0007journal_volume
47pub_type
杂志文章abstract::Choroidal neovascularization (CNV) is one of the most frequent causes of severe and progressive vision loss, while its pathogenesis is still poorly understood. Focal adhesion kinase (FAK), a non-receptor tyrosine kinase, plays a crucial role in linking signals initiated by both the extracellular matrix (ECM) and solub...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.11.034
更新日期:2009-05-01 00:00:00
abstract::The aim of this study was to elucidate the expression of chemokines, their role and regulation in bacterial corneal infection using three bacterial strains (Pseudomonas. aeruginosa- invasive, cytotoxic and contact lens induced acute red eye strains) which have been shown to produce three distinct patterns of corneal d...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(02)00270-1
更新日期:2003-02-01 00:00:00
abstract::Müller cells can completely repair retinal injury by acting as endogenous stem/progenitor cells in lower-order vertebrates. However, a safe and effective approach to activate progenitor potential of retinal Müller cells in higher-order vertebrates, which rarely re-enter the cell cycle, is a bottleneck problem. In the ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.01.008
更新日期:2015-03-01 00:00:00
abstract::High-mobility group Box-1 (HMGB1) is known to be released from injured cells and to induce an inflammatory response. Although HMGB1 was reported to mediate ischemia-reperfusion injury of the brain, its role in glutamate excitotoxicity of the retina remains controversial. Here, the authors demonstrated the evidence tha...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.06.003
更新日期:2015-08-01 00:00:00
abstract::The genetically encoded green fluorescent protein-based calcium sensor, GCaMP, has been used to detect calcium transients and report neuronal activity. We evaluated the specificity of GCaMP3 expression to retinal ganglion cells (RGCs) of the transgenic Thy1-GCaMP3 mouse line in healthy control animals and in those aft...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108297
更新日期:2021-01-01 00:00:00
abstract::Two previous studies have shown that N(G)-nitro-L-arginine methyl ester (L-NAME), an inhibitor of neuronal nitric oxide synthase, protects retinas of albino rats and mice from damaging levels of light. The aims of the present study were two-fold: (1) to confirm the protective effect of L-NAME on wild type albino rats ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(02)00334-2
更新日期:2003-04-01 00:00:00
abstract::All aqueous humor draining through the conventional outflow pathway must cross the endothelium of Schlemm's canal (SC), likely by passing through micron-sized transendothelial pores. SC pores are non-uniformly distributed along the inner wall endothelium, but it is unclear how the distribution of pores relates to the ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.11.008
更新日期:2015-01-01 00:00:00
abstract::This lecture summarizes studies on the effects of some of the neuropeptides which seem to be present in somatosensory and autonomic nerves in the uvea. Release of these peptides is likely to explain nerve induced effects in the eye which are not due to classical transmitters. Trigeminal nerve fibres in the eye seem to...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/0014-4835(91)90138-5
更新日期:1991-07-01 00:00:00
abstract::A newly-developed fibre-optic sensor (wavelength of 670 nm) was used to measure the index gradient in 16 human lenses ranging in age from 38 to 78 years. Two of these lenses had nuclear cataract. The refractive index, calculated from the proportion of reflected light at the sensor/sample interface, appeared to increas...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(05)80097-1
更新日期:1995-04-01 00:00:00
abstract::We describe a model of acute intraocular pressure (IOP) elevation in the mouse eye that induces reversible loss of inner retinal function associated with oxidative stress, glial cell activation and minimal loss of retinal ganglion cell (RGC) number. Young healthy mouse eyes recover inner retinal function within 7-days...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.03.006
更新日期:2015-12-01 00:00:00
abstract::This study of lens protein composition found that some cytoskeletal proteins were degraded during the earliest stages of cataract formation. Cataract was induced in 13-14 day old rats by a single subcutaneous injection of sodium selenite (19 mumol kg-1). By 24 hr after the injection of selenite, the ratio of insoluble...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0220
更新日期:1997-03-01 00:00:00
abstract::Human retinal pigmented epithelial (RPE) cells cultured on plastic, unlike RPE in situ, often fail to density arrest, do not produce melanin, and do not express mRNA for cellular retinaldehyde binding protein (CRALBP). When human RPE are cultured on the extracellular matrix produced by bovine corneal endothelial cells...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1993.1158
更新日期:1993-11-01 00:00:00
abstract::In ocular surface inflammatory diseases, such as dry eye disease, long-term symptom relief requires targeting the inflammation itself rather than treating only the surface-associated dryness with artificial tears. Therefore, we included an anti-inflammatory agent in an unpreserved liposome-based (LP) formulation used ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2016.11.010
更新日期:2017-01-01 00:00:00
abstract::Dark-rearing has been found to slow the rate of retinal degeneration in albino P23H but not S334ter mutant rhodopsin transgenic (Tg) rats. Since eye pigmentation has the same protective slowing effect as dark-rearing in RCS rats, we examined whether eye pigmentation has a comparable slowing effect in the different mut...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107755
更新日期:2019-10-01 00:00:00
abstract::Limbal transplants in humans show a high rate of rejection even under local and systemic immunotherapy. In order to test immunomodulatory treatments a new limbal transplant model in the rat was developed using enhanced green fluorescent protein (E-GFP) as marker for follow-up. Sixty E-GFP-positive limbal transplants f...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.06.006
更新日期:2006-11-01 00:00:00
abstract::The TGFβ/BMP signaling pathways are involved in glaucomatous damage to the trabecular meshwork (TM) leading to elevated intraocular pressure (IOP), which is a major risk factor for the development and progression of glaucoma. The BMP antagonist gremlin is elevated in glaucomatous TM cells and tissues and can directly ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.05.011
更新日期:2013-08-01 00:00:00
abstract::Research in animal models have shown that exposing the eye to positive or negative spectacle lenses can lead to predictable changes in eye growth. Recent research indicates that brief periods (1-2 h) of monocular defocus results in small, but significant changes in axial length and choroidal thickness of human subject...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.03.019
更新日期:2019-05-01 00:00:00
abstract::Although primary cultures of human lens epithelial (HLE) cells provide important information concerning the role of epithelium in normal lens and cataract formation, the lack of a cell line precludes a broad range of studies on the metabolism and molecular biology of these cells. We have, therefore developed an HLE ce...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0551
更新日期:1998-11-01 00:00:00
abstract::The majority of dry eye symptoms are due to a chronic inflammation of the lacrimal functional unit resulting in a loss of tear film integrity and normal function. This leads to a reduction in the ability of the ocular surface to respond to environmental challenges. The underlying cause of tear film dysfunction is the ...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2003.09.003
更新日期:2004-03-01 00:00:00
abstract::The purpose of this study was to evaluate specific keratocyte phenotypes (keratocyte, fibroblast, myofibroblast) for cell contractility and ability to contract extracellular matrix. Rabbit keratocyte phenotype was modulated by exposure to optimal proliferative doses of IGF-I, IL-1alpha, FGF2, PDGF-AB, and TGFbeta(1). ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(03)00188-x
更新日期:2003-11-01 00:00:00
abstract::Measurement of intraocular pressure (IOP) is an essential tool in monitoring glaucoma. Single IOP assessments during clinical routine examinations represent punctual values and are not able to identify IOP fluctuations and spikes. Telemetric IOP measurements are able to monitor IOP during the day and night, and are lo...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.10.020
更新日期:2018-01-01 00:00:00
abstract::The mechanical, histological and pharmacological properties of the isolated cat ophthalmociliary artery are reported and compared for passively stretched ring segments from three locations on the artery. Ring segments were mounted in a modified myograph system after the method of Hogestatt, Andersson and Edvinsson (19...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(92)90047-v
更新日期:1992-03-01 00:00:00
abstract::The interaction of keratocytes with extracellular matrix components plays an important role in the maintenance of corneal transparency and shape as well as in the healing of corneal wounds. In particular, the interaction of these cells with collagen and cell-mediated collagen contraction contribute to wound closure. E...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.107933
更新日期:2020-02-01 00:00:00
abstract::microPx-11, a ferriheme undecapeptide proteolytic degradation product of cytochrome C is shown to be a peroxidase with broad specificity degrading H2O2 and tertiary butyl hydroperoxide. It is also capable of effectively eliminating superoxide and hydroxyl radical. The peroxidase loses activity in the presence of perox...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0336
更新日期:1997-10-01 00:00:00
abstract::Previously, we demonstrated that the inwardly rectifying K(+) (Kir) channel subunit Kir7.1 is highly expressed in bovine and human retinal pigment epithelium (RPE). The purpose of this study was to determine whether any of the 14 other members of the Kir gene family are expressed in native human RPE. Conventional reve...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2008.05.010
更新日期:2008-09-01 00:00:00
abstract::The retinal pigment epithelium (RPE) constitutes a monolayer of cuboidal cells that interact apically with the interphotoreceptor matrix (IPM) and outer segments of the photoreceptor cells and basally with the subjacent Bruch's membrane. This highly polarized structure is maintained by the cytoskeleton of individual c...
journal_title:Experimental eye research
pub_type: 杂志文章,评审
doi:10.1016/j.exer.2013.09.015
更新日期:2014-09-01 00:00:00
abstract::Aldose reductase (AR) is implicated in some of the disabling complications of diabetes, including neuropathy, retinopathy and cataracts. Our studies are aimed at further clarifying the role of AR in diabetes and facilitating the design of new classes of potent, specific AR inhibitors by gaining an understanding of the...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(89)90048-1
更新日期:1989-09-01 00:00:00
abstract::The results reported here clearly document changes in the properties and the organization of fiber-cell membrane lipids that occur with age, based on electron paramagnetic resonance (EPR) analysis of lens membranes of clear lenses from donors of age groups from 0 to 20, 21 to 40, and 61 to 80 years. The physical prope...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.01.018
更新日期:2015-03-01 00:00:00
abstract::A number of different agents, such as growth factors, cytokines and phorbol esters have been shown to modulate trabecular meshwork cell function. These studies were designed to evaluate the role extracellular signal-regulated kinase (ERK) pathway plays in mediating the responses to platelet-derived growth factor-BB (P...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.2001.1007
更新日期:2001-07-01 00:00:00
abstract::Recent studies have shown that lactate coupled water flux is the underlying mechanism of the corneal endothelial pump, which is highly dependent on the presence of bicarbonate. In this study we test the hypothesis that the increased intracellular pH (pHi) caused by bicarbonate stimulates glycolytic activity and the pr...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108193
更新日期:2020-10-01 00:00:00