Ultrastructure of the trabecular meshwork in untreated cases of primary open-angle glaucoma (POAG).


:Thirty-one trabeculectomy specimens of patients suffering from primary open-angle glaucoma (POAG) who had received no, or only minor, medication prior to surgery were ultrastructurally and quantitatively analysed. Most of the specimens revealed thickened trabeculae, increased amounts of plaque-material deposited within the cribriform layer and an abundance of long spacing (lattice) collagen. The uveal meshwork was partly deprived of cells whereas the cribriform layer often contained numerous enlarged, light cells with many small mitochondria and lysosomes but no prominent endoplasmic reticulum or Golgi complexes. The quantitation of sheath-deprived plaque material (SD plaques) in a defined area of inner and outer wall of Schlemm's canal showed no significant difference between the untreated cases studied here and the treated ones studied previously. However, both groups had significantly higher amounts of SD plaque material in the inner wall than normal controls of a similar age range.


Exp Eye Res


Rohen JW,Lütjen-Drecoll E,Flügel C,Meyer M,Grierson I




Has Abstract


1993-06-01 00:00:00














  • Cold inhibits neurite outgrowth from single retinal ganglion cells isolated from adult goldfish.

    abstract::We have studied the growth of neurites from single retinal ganglion cells isolated from adult goldfish and maintained under various primary cell culture conditions. In 10% Leibovitz's L-15 medium at 23 degrees C, these ganglion cells remained viable for up to 10 days and generated extensive fields of neurites. We foun...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Ishida AT,Cheng MH

    更新日期:1991-02-01 00:00:00

  • Effects of basic fibroblast growth factor on the retinal degeneration of the mi(vit)/mi(vit) (vitiligo) mouse: a morphologic and electrophysiologic study.

    abstract::Basic fibroblast growth factor (bFGF) has been shown to rescue dying photoreceptor cells in the RCS rat, a model with a genetic defect of the RPE that impairs outer segment phagocytosis. The purpose of the present study was to determine whether intravitreal injection of bFGF would have a similar effect on photorecepto...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Smith SB,Titelman R,Hamasaki DI

    更新日期:1996-11-01 00:00:00

  • Tissue culture of rabbit ciliary body epithelial cells on permeable supports.

    abstract::The aqueous humor is produced by the epithelium of the ciliary body, a complex structure encircling the anterior segment of the eye. Aqueous humor production occurs by active transport, but the mechanism of this process is not understood. To produce a preparation in which active transport can be investigated, we have ...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Cilluffo MC,Fain MJ,Fain GL

    更新日期:1993-11-01 00:00:00

  • miR-365 promotes diabetic retinopathy through inhibiting Timp3 and increasing oxidative stress.

    abstract::miRs play critical roles in oxidative stress-related retinopathy pathogenesis. miR-365 was identified in a previously constructed library from glyoxal-treated rat Müller cell. This report explores epigenetic alterations in Müller cells under oxidative stress to develop a novel therapeutic strategy. To examine the miR-...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Wang J,Zhang J,Chen X,Yang Y,Wang F,Li W,Awuti M,Sun Y,Lian C,Li Z,Wang M,Xu JY,Jin C,Tian H,Gao F,Zhang J,Sinha D,Lu L,Xu GT

    更新日期:2018-03-01 00:00:00

  • Innervation pattern of the preocular human central retinal artery.

    abstract::The central retinal artery (CRA) is the main vessel for inner retinal oxygen and nutrition supply. While the intraocular branches lack autonomic innervation, the innervation pattern of the extra-ocular part of this vessel along its course within the optic nerve is poorly investigated. This part however is essential fo...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Bergua A,Kapsreiter M,Neuhuber WL,Reitsamer HA,Schrödl F

    更新日期:2013-05-01 00:00:00

  • Rat BMSCs initiate retinal endogenous repair through NGF/TrkA signaling.

    abstract::Müller cells can completely repair retinal injury by acting as endogenous stem/progenitor cells in lower-order vertebrates. However, a safe and effective approach to activate progenitor potential of retinal Müller cells in higher-order vertebrates, which rarely re-enter the cell cycle, is a bottleneck problem. In the ...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Jian Q,Li Y,Yin ZQ

    更新日期:2015-03-01 00:00:00

  • A developmental study of interphotoreceptor retinoid-binding protein (IRBP) in single and double homozygous rd and rds mutant mouse retinae.

    abstract::Interphotoreceptor retinoid-binding protein (IRBP) was studied using immunochemical and immunocytochemical techniques in retinae of mice with allelic combinations at the rd and rds loci at different stages of development and degeneration. Until postnatal day 7 (P7), IRBP is located intracellularly in developing retina...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: van Veen T,Ekstrom P,Wiggert B,Lee L,Hirose Y,Sanyal S,Chader GJ

    更新日期:1988-08-01 00:00:00

  • Idiopathic retinal degeneration in the dog: differential patterns of [3H]uridine incorporation and HIOMT-like immunoreactivity in surviving photoreceptors.

    abstract::Photoreceptor cell pathology was investigated in an 8-yr-old mixed-breed dog which had displayed visual symptoms of 1 month duration. An electroretinogram detected no light-evoked responses. Light and electron microscopic features showed marked thinning and atrophy of the outer both the tapetal and non-tapetal retina ...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Wiechmann AF,Font RL,Hollyfield JG

    更新日期:1991-03-01 00:00:00

  • RPE conditioned medium stimulates photoreceptor cell survival, neurite outgrowth and differentiation in vitro.

    abstract::In the present study we have investigated retinal pigment epithelium-photoreceptor cell interactions in vitro, and their contributions to photoreceptor cell survival and differentiation. Preparations enriched for intact photoreceptor cells from neonatal rat retina were grown in either serum-free medium supplemented wi...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Gaur VP,Liu Y,Turner JE

    更新日期:1992-05-01 00:00:00

  • A rabbit lens epithelial cell line supports expression of an exogenous crystallin gene characteristic of lens fiber cell differentiation.

    abstract::Cell lines derived from the lens generally fail to maintain synthesis of crystallins in long-term culture. Here we demonstrate that the N/N1003A line of undifferentiated lens epithelial cells, derived from a newborn rabbit, does not produce detectable levels of alpha-, beta- or gamma-crystallin transcripts, yet is cap...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Meakin SO,Reddan JR,Tsui LC,Breitman ML

    更新日期:1989-01-01 00:00:00

  • Differential immunogold-dextran labeling of bovine and frog rod and cone cells using monoclonal antibodies against bovine rhodopsin.

    abstract::Eleven monoclonal antibodies against different segments of bovine rhodopsin were used with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal photoreceptor cells for visualization by transmission electron microscopy. Antibodies against the C-terminus, F1-F2 loop and N-terminus of rho...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Hicks D,Molday RS

    更新日期:1986-01-01 00:00:00

  • Distribution of cathepsin D in human eyes with or without age-related maculopathy.

    abstract::Cathepsin D is a ubiquitous enzyme which plays an important role in the catabolism of proteins. Enzymatic studies showed that cathepsin D is the most important lysosomal enzyme in the proteolysis of opsin. The importance of cathepsin D in the lysosomal digestion of phagocytosed photoreceptor outer segments by the reti...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Rakoczy PE,Sarks SH,Daw N,Constable IJ

    更新日期:1999-10-01 00:00:00

  • On the reactivity of corneal collagen and subcomponent C1q of the complement system with human platelets and IgG-coated latex particles.

    abstract::Collagen was isolated from bovine cornea and tested for reactivity towards platelets and IgG-coated polystyrene latex particles. The corneal collagen caused a dose- and temperature-dependent platelet aggregation in all human platelet-rich plasmas studied. As little as 0 . 31 micrograms of purified corneal collagen cou...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Csako G,Suba EA,Herp A

    更新日期:1983-03-01 00:00:00

  • Preclinical pharmacology, ocular tolerability and ocular hypotensive efficacy of a novel non-peptide bradykinin mimetic small molecule.

    abstract::We sought to characterize the ocular pharmacology, tolerability and intraocular pressure (IOP)-lowering efficacy of FR-190997, a non-peptidic bradykinin (BK) B2-receptor agonist. FR-190997 possessed a relatively high receptor binding affinity (Ki = 27 nM) and a high in vitro potency (EC50 = 18.3 ± 4.4 nM) for inositol...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Sharif NA,Li L,Katoli P,Xu S,Veltman J,Li B,Scott D,Wax M,Gallar J,Acosta C,Belmonte C

    更新日期:2014-11-01 00:00:00

  • Age-related changes in fibre differentiation of rat lens epithelial cells in vitro.

    abstract::Epithelial cells in explants from rat lenses differentiate into fibre cells when cultured with retina conditioned medium (RCM). This differentiation is characterised by cell elongation, the accumulation of alpha-crystallin and the onset of synthesis of the fibre specific beta- and gamma-crystallins. To determine the e...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Richardson NA,McAvoy JW

    更新日期:1988-02-01 00:00:00

  • Human lens epithelial cell line.

    abstract::Although primary cultures of human lens epithelial (HLE) cells provide important information concerning the role of epithelium in normal lens and cataract formation, the lack of a cell line precludes a broad range of studies on the metabolism and molecular biology of these cells. We have, therefore developed an HLE ce...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Ibaraki N,Chen SC,Lin LR,Okamoto H,Pipas JM,Reddy VN

    更新日期:1998-11-01 00:00:00

  • Effect of a contact lens on mouse retinal in vivo imaging: Effective focal length changes and monochromatic aberrations.

    abstract::For in vivo mouse retinal imaging, especially with Adaptive Optics instruments, application of a contact lens is desirable, as it allows maintenance of cornea hydration and helps to prevent cataract formation during lengthy imaging sessions. However, since the refractive elements of the eye (cornea and lens) serve as ...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Zhang P,Mocci J,Wahl DJ,Meleppat RK,Manna SK,Quintavalla M,Muradore R,Sarunic MV,Bonora S,Pugh EN Jr,Zawadzki RJ

    更新日期:2018-07-01 00:00:00

  • Sulfhydryl binding reagents increase the conductivity of the light-sensitive channel and inhibit phototransduction in retinal rods.

    abstract::The mechanisms by which sulfhydryl (SH-) binding reagents modulate the light-sensitive conductance of retinal rods were investigated by current recording from single rods, by patch clamp recording from the plasma membrane of the rod outer segment (ROS), and by biochemical study of their effects on the light-induced hy...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Donner K,Hemilä S,Kalamkarov G,Koskelainen A,Pogozheva I,Rebrik T

    更新日期:1990-07-01 00:00:00

  • MicroRNA-7a regulates Müller glia differentiation by attenuating Notch3 expression.

    abstract::miRNA-7a plays critical roles in various biological aspects in health and disease. We aimed to reveal roles of miR-7a in mouse retinal development by loss- and gain-of-function analyses of miR-7a. Plasmids encoding miR-7a or miR-7a-decoy (anti-sense miR-7a) were introduced into mouse retina at P0, and the retina was c...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Baba Y,Aihara Y,Watanabe S

    更新日期:2015-09-01 00:00:00

  • Topography of retinal damage in light-exposed albino rats.

    abstract::Previous studies have shown that retinal damage induced by damaging light exposure is more severe in superior retina than inferior retina when measured along the vertical meridian of eyes. However, the extent of retinal damage over all retinal regions is not clear. Albino rats were exposed to bright light (5000 lux fo...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Tanito M,Kaidzu S,Ohira A,Anderson RE

    更新日期:2008-09-01 00:00:00

  • Lectin binding patterns in developing canine retina.

    abstract::Developing canine retina, fixed in Bouin's or Tellyesnizky's acetic alcoholic formalin, was examined with a battery of biotinylated lectins. ConA and WGA consistently bound to all retinal layers including the tapetum cellulosum, retinal pigment epithelium, inner and outer segments, plexiform layers and ganglion cells....

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Whiteley HE,Scott JR

    更新日期:1990-10-01 00:00:00

  • Specific racemization and isomerization of the aspartyl residue of alphaA-crystallin due to UV-B irradiation.

    abstract::We have reported that the aspartyl (Asp)-151 residue in alphaA-crystallin in human eye lens was inverted to the D-isomer and isomerized to beta-Asp residue with age. We report here that ultraviolet (UV)-B irradiation induces the racemization and isomerization of the Asp-151 residue of alphaA-crystallin from lenses of ...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Fujii N,Momose Y,Ishibashi Y,Uemura T,Takita M,Takehana M

    更新日期:1997-07-01 00:00:00

  • Inductive differentiation of conjunctival goblet cells by γ-secretase inhibitor and construction of recombinant conjunctival epithelium.

    abstract::γ-secretase inhibitor has been shown to promote intestinal goblet cell differentiation. We now demonstrated that the in vitro addition of γ-secretase inhibitor in the culture of human conjunctival epithelial cells significantly promoted the differentiation of conjunctival goblet cells with typical droplet-like phenoty...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Tian L,Qu M,Wang Y,Duan H,Di G,Xie L,Zhou Q

    更新日期:2014-06-01 00:00:00

  • Effect of aclacinomycin A on in vitro cultures of porcine lens epithelial cells.

    abstract::As a potential drug for the prevention of secondary cataract formation (SCF), we investigated the effect of Aclacinomycin A (ACM) on the growth of cultures of porcine lens epithelial cells in vitro. ACM is an anthracycline that has been used in the treatment of acute myeloid leukemia in the human for many years. It ha...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Schmidt JF,Meyer JH,Fluegel B,Hansen LL,Loeffler KU

    更新日期:1998-01-01 00:00:00

  • Natural, high-mannose glycoproteins inhibit ROS binding and ingestion by RPE cell cultures.

    abstract::Previous studies have suggested that a mannose receptor mediates the phagocytic uptake of effete rod outer segments by retinal pigment epithelial cells. In the present study, the effect of adding a soluble ligand for the mannose receptor, horseradish peroxidase, was examined. Cultured retinal pigment epithelial cells ...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Lutz DA,Guo Y,McLaughlin BJ

    更新日期:1995-10-01 00:00:00

  • Transdifferentiation of chicken retinal pigmented epithelial cells in serum-free culture.

    abstract::A serum-free culture of chicken retinal pigmented epithelial cells has been established in order to analyse how cell-substrate interactions or environmental factors affect the process of transdifferentiation into lens cells from pigmented epithelial cells. The serum-free culture medium for chicken pigmented epithelial...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Kosaka J,Watanabe K,Eguchi G

    更新日期:1992-08-01 00:00:00

  • The effect of white light and UV-A on the green autofluorescence of the rabbit lens in vivo.

    abstract::The part of the light spectrum which is responsible for an increase of lenticular green autofluorescence upon white light exposure was determined for the rabbit. The increase of autofluorescence as a function of the light energy was measured, to assess a possible threshold energy for lens alterations. Thirteen rabbits...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Van Vreeswijk H,Boets EP,Van Best JA

    更新日期:1993-03-01 00:00:00

  • Prevention of experimental autoimmune uveoretinitis by blockade of osteopontin with small interfering RNA.

    abstract::Osteopontin (OPN) is elevated during the progression of experimental autoimmune uveoretinitis (EAU) in C57BL/6 (B6) mice. Furthermore, EAU symptoms are ameliorated in OPN knockout mice or in B6 mice treated with anti-OPN antibody (M5). Recently, OPN has been shown to promote the Th1 response not only in the extracellu...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Iwata D,Kitamura M,Kitaichi N,Saito Y,Kon S,Namba K,Morimoto J,Ebihara A,Kitamei H,Yoshida K,Ishida S,Ohno S,Uede T,Onoé K,Iwabuchi K

    更新日期:2010-01-01 00:00:00

  • The utility of massively parallel sequencing for posterior polymorphous corneal dystrophy type 3 molecular diagnosis.

    abstract::The aim of this study was to identify the molecular genetic cause of disease in posterior polymorphous corneal dystrophy (PPCD) probands of diverse origin and to assess the utility of massively parallel sequencing in the detection of ZEB1 mutations. We investigated a total of 12 families (five British, four Czech, one...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Dudakova L,Evans CJ,Pontikos N,Hafford-Tear NJ,Malinka F,Skalicka P,Horinek A,Munier FL,Voide N,Studeny P,Vanikova L,Kubena T,Rojas Lopez KE,Davidson AE,Hardcastle AJ,Tuft SJ,Liskova P

    更新日期:2019-05-01 00:00:00

  • Inhibition of the acute ocular responses to nitrogen mustard by colchicine.

    abstract::Colchicine, a naturally occurring alkaloid, inhibits both fast and slow axoplasmic transport by prevention of microtubule assembly. We have examined the influence of colchicine on the neurogenically mediated acute inflammatory responses to topically administered nitrogen mustard and formaldehyde on the albino rabbit e...

    journal_title:Experimental eye research

    pub_type: 杂志文章


    authors: Williams RN,Bhattacherjee P

    更新日期:1984-12-01 00:00:00