Abstract：
:Aceclidine can dissociate accommodative and outflow facility responses in monkeys and humans. We sought to determine if different muscarinic receptor subtypes control outflow facility, accommodative and pupillary responses to aceclidine in the living rhesus monkey eye, as a possible basis for this separation. Each eye was cannulated with one branched and one unbranched needle. Baseline measurements (of outflow facility by two-level constant pressure perfusion; refraction by Hartinger coincidence refractometry and pupil diameter by vernier calipers) were recorded after anterior chamber exchange with (one eye) or without (opposite eye), muscarinic receptor subtype antagonist (pirenzepine, AF-DX 116 or 4-DAMP). The eyes were then exchanged a second time with these solutions plus added aceclidine. The response to aceclidine (the differences from baseline) in the presence or absence of antagonist were compared. The M3 muscarinic receptor subtype antagonist 4-DAMP was the most potent inhibitor of all three responses to aceclidine. The dissociation of accommodative, outflow facility and miotic responses to aceclidine in rhesus monkeys does not appear to be due to differences in the muscarinic receptor subtypes that can currently be distinguished pharmacologically.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Gabelt BT,Kaufman PLdoi
10.1006/exer.1994.1057subject
Has Abstractpub_date
1994-05-01 00:00:00pages
623-30issue
5eissn
0014-4835issn
1096-0007pii
S0014-4835(84)71057-8journal_volume
58pub_type
杂志文章abstract：:Experiments were performed using [1-14C]PGH2 endoperoxide as substrate to assess which, if any, of the PGH2 utilizing enzymes were present in rat lens microsomes. After a 15 min incubation, prostaglandins (PGs) were extracted, separated by thin-layer chromatography and labeled PGs located by autoradiography. The bands...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80010-6
更新日期：1987-02-01 00:00:00
abstract：:Lens development and transparency have been hypothesized to depend on intercellular gap junction channels, consisting of alpha3 (Cx46) and alpha8 (Cx50) connexin subunits, to transport metabolites, secondary messages and ions between lens cells. To evaluate this hypothesis, we have generated alpha3(-/-) alpha8(-/-) do...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.03.013
更新日期：2006-09-01 00:00:00
abstract：:Retinal degeneration (RD) results from photoreceptor apoptosis. Cell transplantation, one potential therapeutic approach, requires expandable stem cells that can form mature photoreceptors when differentiated. Freshly dissociated primary retinal cells from postnatal day 2-6 (PN2-6) mouse retina can give rise, post-tra...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.07.003
更新日期：2010-10-01 00:00:00
abstract：:The trabecular meshwork is considered a smooth muscle like tissue contributing to aqueous outflow regulation and thus to regulation of intraocular pressure. An elevation in intraocular pressure is one of the greatest risk factors for most forms of glaucoma. We assume that contraction of trabecular meshwork reduces aqu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.12.013
更新日期：2005-06-01 00:00:00
abstract：:miRNA-7a plays critical roles in various biological aspects in health and disease. We aimed to reveal roles of miR-7a in mouse retinal development by loss- and gain-of-function analyses of miR-7a. Plasmids encoding miR-7a or miR-7a-decoy (anti-sense miR-7a) were introduced into mouse retina at P0, and the retina was c...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.06.022
更新日期：2015-09-01 00:00:00
abstract：:Due to the high toxicity of aminoglycoside antibiotics, many authors prefer to use third generation cephalosporines in the prophylaxis and treatment of intraocular infections. The aim of the present study was to determine safe ceftazidime levels in anterior chamber irrigation solution. Twenty-two eyes of 12 white New ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0167
更新日期：1996-12-01 00:00:00
abstract：:Human Kv3.3/KCNC3 is a Shaw-type potassium channel that has been mapped to chromosome 19q13.3-13.4. Complete mouse and rat Kv3.3 cDNA coding sequences have been published, yet the human Kv3.3 cDNA has remained incomplete for years. We report here for the first time the amino acid sequence for hKv3.3 and the electrophy...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0796
更新日期：2000-03-01 00:00:00
abstract：:Transport of circulating sulfur amino acids (SAA) into the lens epithelium and de novo glutathione (GSH) synthesis were studied in the perfused guinea-pig eye. Plasma-to-aqueous transfer of SAA was in their intact form (> or = 98%) and comparable with sucrose (an extracellular marker) within 30 min. The unidirectional...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0260
更新日期：1997-04-01 00:00:00
abstract：:The distribution of hyaluronan (HA) in the posterior eye wall from the vitreous through the sclera, with special consideration to localization in the retina and interphotoreceptor matrix (IPM), was evaluated in mouse tissues using an HA specific probe (bHABC, biotinylated hyaluronan binding complex). The vitreous body...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0369
更新日期：1997-11-01 00:00:00
abstract：:Although primary cultures of human lens epithelial (HLE) cells provide important information concerning the role of epithelium in normal lens and cataract formation, the lack of a cell line precludes a broad range of studies on the metabolism and molecular biology of these cells. We have, therefore developed an HLE ce...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0551
更新日期：1998-11-01 00:00:00
abstract：:The overall importance of the peak or the mean serum concentrations as predictors of ocular drug penetration is unknown. To address this fundamental question with an agent which shows promise as adjunctive therapy in the treatment of endophthalmitis, we studied the penetration of ciprofloxacin into the aqueous and vit...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0101
更新日期：1996-08-01 00:00:00
abstract：:Stroboscopic luminance flicker has been found to prevent the increase in eye growth normally associated with form deprivation through the release of retinal dopamine. In this study, we sought to investigate whether dopamine plays a role in the decreased growth observed with 2 Hz sine-wave luminance flicker and increas...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107822
更新日期：2019-12-01 00:00:00
abstract：:SPACRCAN is a novel proteoglycan present in the interphotoreceptor matrix (IPM) of the rat and human retina that resists aqueous extraction through its binding to hyaluronan. The purpose of this study was: to clone mouse Spacrcan; to characterize the promoter elements; to define the deduced amino acid sequence; to est...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00273-7
更新日期：2003-01-01 00:00:00
abstract：:Hyaluronan (HA) distribution in the posterior eye wall from the vitreous through the sclera, with special consideration to localization in the retina and interphotoreceptor matrix (IPM), was evaluated in human, bovine, guinea pig, dog, rat and mouse tissues using a specific probe for HA (bHABC, biotinylated hyaluronan...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0422
更新日期：1998-02-01 00:00:00
abstract：:The eye lens is a transparent and avascular organ in the front of the eye that is responsible for focusing light onto the retina in order to transmit a clear image. A monolayer of epithelial cells covers the anterior hemisphere of the lens, and the bulk of the lens is made up of elongated and differentiated fiber cell...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2016.03.005
更新日期：2017-03-01 00:00:00
abstract：:The purpose of this study was to examine the localization and relative levels of vascular endothelial growth factor (VEGF; an angiogenic factor) and pigment epithelium-derived factor (PEDF; an antiangiogenic factor) in aged human choroid and to determine if the localization or their relative levels changed in age-rela...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.05.007
更新日期：2006-01-01 00:00:00
abstract：:Sex steroids impact regulation of the ocular surface tissues and thus influence dry eye. Most tissues in the human body synthesise and metabolise active sex steroids at levels required by the tissue. This is likely to also be the case for humans in ocular surface tissues. This study investigated the presence and quant...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.11.015
更新日期：2019-02-01 00:00:00
abstract：:We have investigated the biochemical and cell biological basis of the reported beneficiary effects of the leaf extracts of the plant Ginkgo biloba, which has been used as a possible ophthalmic drug. The antioxidant, antimicrobial, anti-apoptotic and cytoprotective properties of the standardized extract called EGb761 w...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：
更新日期：2002-10-01 00:00:00
abstract：:The purpose of these experiments was to develop a hypothesis to explain activation of m-calpain in cataractogenesis observed in rodents. The in vitro model used to study m-calpain activation was to correlate breakdown of the 'reporter' protein alpha-crystallin with the appearance of activated m-calpain using protein s...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0234
更新日期：1997-04-01 00:00:00
abstract：:Retinoblastoma generally occurs before 5 years of age and often requires enucleation (surgical removal of one eye) of the cancerous eye. We have previously shown using behavioural methods that this disruption in binocular vision during the critical period of visual development results in impaired face perception. In t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.09.013
更新日期：2019-06-01 00:00:00
abstract：:Recently, a unique distribution, namely a reduction of macular pigment optical density (MPOD) within the central retina with a surrounding ring-like structure of preserved MPOD at about 6 degrees eccentricity was suggested to be a common finding in macular telangiectasia (MacTel) type 2. In order to quantify this redu...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.02.006
更新日期：2009-06-15 00:00:00
abstract：:Human, monkey, and bovine retinal pigment epithelial (RPE) cells exhibit an M-type K+ current, which in many other cell types is mediated by channels composed of KCNQ α-subunits and KCNE auxiliary subunits. Recently, we demonstrated the expression of KCNQ1, KCNQ4, and KCNQ5 in the monkey RPE. Here, we investigated the...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：:The retinal pigment epithelial cell has several important functions, one of which is the phagocytosis of photoreceptor outer segments which are discarded diurnally. We previously provided evidence in human retinal pigment epithelium that CD36, an 88 kDa integral membrane glycoprotein, participates in the phagocytosis ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0177
更新日期：1997-01-01 00:00:00
abstract：:All aqueous humor draining through the conventional outflow pathway must cross the endothelium of Schlemm's canal (SC), likely by passing through micron-sized transendothelial pores. SC pores are non-uniformly distributed along the inner wall endothelium, but it is unclear how the distribution of pores relates to the ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.11.008
更新日期：2015-01-01 00:00:00
abstract：:We examined the expression of mGlu2/3 metabotropic glutamate receptors in the retina of the goldfish Carassius auratus. mGlu2/3 receptors were expressed in all retinal layers internal to the photoreceptor layer, particularly in the outer and inner nuclear layers. Although the goldfish brain is able to tolerate prolong...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.11.008
更新日期：2007-03-01 00:00:00
abstract：:The effects of metabolic inhibitors on the apical and basal membrane potentials were studied in the isolated retinal pigment epithelium of the lizard Gekko gekko. Adding dinitrophenol or cyanide or cooling the tissue to 15 degrees C first depolarized the apical membrane and then hyperpolarized the basal membrane. The ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90015-m
更新日期：1990-01-01 00:00:00
abstract：:Misregulation of the innate immune response and other immune-related processes have been suggested to play a critical role in the pathogenesis of a number of different neurodegenerative diseases, including age related macular degeneration. In an animal model for photoreceptor degeneration, several genes of the innate ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.08.017
更新日期：2007-01-01 00:00:00
abstract：:We developed an approach to generate a three-dimensional map that facilitates the assessment of epithelial nerve density in different corneal areas to define aging and gender influence on human corneal nerve architecture. Twenty-eight fresh human eyes from 14 donors of different ages were studied. Corneal nerves were ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.07.007
更新日期：2010-10-01 00:00:00
abstract：:The biomechanical environment within the eye is of interest in both the regulation of intraocular pressure and the loss of retinal ganglion cell axons in glaucomatous optic neuropathy. Unfortunately, this environment is complex and difficult to determine. Here we provide a brief introduction to basic concepts of mecha...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2015.05.024
更新日期：2015-12-01 00:00:00
abstract：:Lactoferrin plays an important role in the defense against infections, including herpes simplex virus (HSV) keratitis. We studied the impact of three single nucleotide polymorphisms in the human lactoferrin gene on the susceptibility to HSV infections of the eye and the severity of such infections. Lactoferrin gene po...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.09.013
更新日期：2008-01-01 00:00:00
