Abstract：
:Glutamine, the end product formed by the glutamine synthetase (GS) reaction, inhibits retinal GS activity in the presence of Mn2+, but not in the presence of Mg2+. In the presence of Mg2+, Mn2+ itself inhibits retinal GS activity. Other compounds which inhibit retinal GS activity significantly are methionine sulfoximine, D-alanine and carbamyl phosphate. Amino acids, such as L-alanine, L-serine and glycine, do not affect the enzyme activity. These amino acids, however, significantly inhibit the enzyme activity when measured on the basis of the glutamyl transferase (GT) reaction. GS isolated from neuronal tissues is regulated differently from that previously reported by others for non-neuronal tissues. The enzyme activity, as measured by GS activity, shows three-fold higher activity with Mg2+ over Mn2+ or Co2+ and on the basis of GT activity, shows about three-fold higher activity with Mn2+ over Mg2+ or Co2+. The optimum pH for the GS reaction lies in the range of 7.2-7.8 and for the GT reaction is 6.4-7.0. Both the GS and GT activities of the enzyme show similar heat stabilities.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Pahuja SL,Reid TWdoi
10.1016/0014-4835(85)90109-5subject
Has Abstractpub_date
1985-01-01 00:00:00pages
75-83issue
1eissn
0014-4835issn
1096-0007pii
0014-4835(85)90109-5journal_volume
40pub_type
杂志文章abstract：:As wounds in stratified epithelia close, the numbers of cells at the leading edge of migration decreases. It is not known whether cells at the leading edge are continually replaced or whether some retain their position at the leading edge over time. Replication-deficient adenovirus carrying the green fluorescent prote...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1115
更新日期：2002-02-01 00:00:00
abstract：:Invagination of the optic vesicle to form the optic cup results in the formation of two apposed layers of neuroepithelium which follow divergent developmental pathways. Changes in the expression of cell surface molecules may be either the cause or result of important inductive signals during this process. We have used...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90088-c
更新日期：1990-11-01 00:00:00
abstract：:We examined the role of Müller (glial) cells in buffering light-evoked changes in extracellular K+ concentration, [K+]o, in the isolated retina of the toad, Bufo marinus. We found evidence for two opposing Müller cell current loops that are generated by a light-evoked increase in [K+]o in the inner plexiform layer. Th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80166-6
更新日期：1992-10-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2016.06.009
更新日期：2017-05-01 00:00:00
abstract：:The integrity of the retinal pigment epithelium, especially that of the macula is essential for the preservation of vision into old age. The chronic exposure to sunlight and peroxidized lipids from phagocytized photoreceptor outer segments imposes a high level of oxidative stress on the retinal tissues, which increase...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1146
更新日期：2002-06-01 00:00:00
abstract：:An extraneous exon-skipping mRNA encodes an altered form of a light-absorbing opsin in human retina and pigment epithelium (RPE). The predicted protein variant differs from full-length RPE-retinal G protein-coupled receptor (RGR) by having an in-frame deletion of exon 6, which contains the entire sixth transmembrane d...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.11.013
更新日期：2006-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.10.006
更新日期：2005-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.07.009
更新日期：2007-10-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1016/s0014-4835(03)00116-7
更新日期：2003-08-01 00:00:00
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journal_title：Experimental eye research
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更新日期：2007-02-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2015.11.023
更新日期：2016-05-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.07.019
更新日期：2007-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2016.07.009
更新日期：2017-05-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(86)80088-4
更新日期：1986-08-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2016.03.005
更新日期：2017-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1013
更新日期：1993-01-01 00:00:00
abstract：:The objective of this study was to determine the cellular localization of P2Y(2) receptor gene expression in rabbit and primate ocular tissues using the technique of non-isotopic in situ hybridization. Fresh frozen whole eye from a New Zealand White rabbit and whole eye and eyelid from a rhesus macaque were cut into 5...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00068-x
更新日期：2003-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00248-8
更新日期：2003-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1077
更新日期：2001-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90059-6
更新日期：1989-06-01 00:00:00
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pub_type： 杂志文章
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更新日期：2013-06-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.10.013
更新日期：2013-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.11.011
更新日期：2008-02-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：2019-08-01 00:00:00
abstract：:A number of deep-sea fish have bright yellow lenses whose coloration is attributable to a variety of largely unidentified short-wave absorbing pigments. Here the pigment of the deep-sea fish Stylephorus chordatus has been isolated and identified by NMR and mass spectroscopy as kynurenine; a pigment also found in the h...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90091-6
更新日期：1992-07-01 00:00:00
abstract：:The purpose of this work was to investigate the effect of c-fos antisense oligonucleotide (c-fos-AS-ON) on proliferative vitreoretinopathy (PVR). Cultures of human retinal pigment epithelial (hRPE) cells were established from adult human corneal donors. These cells were positively stained for cytokeratins. C-fos-AS-ON...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.07.020
更新日期：2006-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.10.022
更新日期：2018-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90202-4
更新日期：1992-08-01 00:00:00
abstract：:Angiotensin II and aldosterone are the main effectors of the renin-angiotensin aldosterone system (RAAS) and have a central role in hypertension as well as cardiovascular and renal disease. The localization of RAAS components within the retina has led to studies investigating the roles of angiotensin II, aldosterone a...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2019.107766
更新日期：2019-10-01 00:00:00
abstract：:The purpose of the current study was to localize the lens membrane protein, MIP 26, in nuclear fiber cells from different regions of aged normal and age-related cataractous human lenses. Adult, juvenile, fetal and embryonic nuclear regions in aged normal and age-related nuclear cataractous lenses were morphologically ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0579
更新日期：1999-01-01 00:00:00
