Abstract：
:Experiments were conducted to evaluate the role played by photoreceptor cells in the accumulation of age pigment, or lipofuscin, in the retinal pigment epithelium (RPE). The age-related accumulation of RPE lipofuscin was compared between rats with hereditary photoreceptor degeneration (RDY) and congenic rats with normal retinas. In the RDY animals, the age-related increase in RPE lipofuscin content was substantially less than in normal controls. This suggests that the photoreceptor cells play a significant role in RPE lipofuscin deposition, although they may not be the sole contributors to RPE lipofuscin formation. Evidence that outer-segment components may be converted into lipofuscin fluorophores was provided by the discovery that in young RDY rats, fragments of outer segments from degenerating photoreceptor cells had fluorescence properties similar to those of RPE lipofuscin. Chloroform-methanol extraction of retina-RPE tissue from young normal and dystrophic rats, and analysis of the chloroform fractions by thin-layer chromatography, revealed three distinct fluorescent components associated with the lipofuscin-like fluorescence of the outer-segment fragments in the RDY rats.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Katz ML,Drea CM,Eldred GE,Hess HH,Robison WG Jrdoi
10.1016/s0014-4835(86)80023-9subject
Has Abstractpub_date
1986-10-01 00:00:00pages
561-73issue
4eissn
0014-4835issn
1096-0007journal_volume
43pub_type
杂志文章abstract：:Purified retinal pigment epithelial cells of bovine eyes have been fractionated by a series of buffer and detergent extractions. The electropherogram of the buffer-insoluble, Triton X-100-soluble fraction (RPE-TS) exhibits a major polypeptide band of M(r) 65 kDa and a variety of minor components. Electrophoretically p...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90008-g
更新日期：1992-12-01 00:00:00
abstract：:Melanopsin is a non-image forming photoreceptor known to be present in the retina and it is considered to have light regulated tasks among other functions. In the present work, melanopsin presence in human lens epithelial cells as well as in human lens tissue is described for the first time. Moreover, studying the con...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.11.019
更新日期：2017-01-01 00:00:00
abstract：:Previous studies have relied on various electrodes or probes to monitor preretinal oxygen tension in an effort to gain insight into retinal oxygenation. In order to corroborate and extend the results of such studies, we developed a relatively non-invasive method of determining preretinal oxygen tension using 19F nucle...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90099-e
更新日期：1992-07-01 00:00:00
abstract：:The Otx2 gene encodes a transcription factor essential for the normal development of brain, cerebellum, pineal gland, and eye. In the retina, Otx2 has essential functions from early embryogenesis to adulthood. As soon as the optic vesicle is formed, the gene is required for retinal pigment epithelium specification. Ot...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.03.007
更新日期：2013-06-01 00:00:00
abstract：:Prostaglandins are widely used to lower intraocular pressure (IOP) as part of the treatment regimen for glaucoma. While FP and EP2 agonists are known to lower IOP, we investigated the ocular hypotensive activity and ocular drug distribution of PF-04475270, a novel EP4 agonist following topical administration in normot...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.05.002
更新日期：2009-11-01 00:00:00
abstract：:Glaucoma challenges the survival of retinal ganglion cell axons in the optic nerve through processes dependent on both aging and ocular pressure. Relevant stressors likely include complex interplay between axons and astrocytes, both in the retina and optic nerve. In the DBA/2J mouse model of pigmentary glaucoma, early...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.11.016
更新日期：2016-09-01 00:00:00
abstract：:It has long been assumed that unstimulated tears are more thoroughly equilibrated with epithelial secretions than stimulated tears, since they are in contact with tarsal, bulbar and corneal surfaces for longer. It was also believed from results with model solutions that soluble mucin is responsible for the observed su...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0600
更新日期：1999-02-01 00:00:00
abstract：:TGFβ2 induces extracellular matrix (ECM) remodeling and alters the cytoskeleton by both the canonical Smad and non-canonical signaling pathways. TGFβ2 regulates the expression of ECM proteins in trabecular meshwork (TM) cells, increases intraocular pressure (IOP) in an ex vivo perfusion organ culture model, and induce...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.10.017
更新日期：2013-11-01 00:00:00
abstract：:Preeclampsia (PE) is a hypertensive complication of pregnancy. Its cause is still unknown and it could be a risk factor for future ophthalmic problems. Retinal vascular bed alterations have been described as a consequence of PE, suggesting a retinopathy. Factors related to angiogenesis and vascular permeability, such ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.107981
更新日期：2020-04-01 00:00:00
abstract：:Changes in the distribution of keratan sulfate in the anterior segment of developing ocular tissue were investigated histochemically and immunohistochemically using human eyes from 5 weeks gestation to 2 years after birth. Enzyme digestion methods with streptomyces hyaluronidase, chondroitinase and keratanase showed t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1018
更新日期：1994-03-01 00:00:00
abstract：:The central retinal artery (CRA) is the main vessel for inner retinal oxygen and nutrition supply. While the intraocular branches lack autonomic innervation, the innervation pattern of the extra-ocular part of this vessel along its course within the optic nerve is poorly investigated. This part however is essential fo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.11.020
更新日期：2013-05-01 00:00:00
abstract：:The lipid composition of retinal pigment epithelial cells was determined for normal cells which have full phagocytic ability and for a genetic variant with impaired phagocytic function. Retinal pigment epithelial cells from 9-14-day-old congenic strains of normal (RCS-rdy+) and dystrophic (RCS-rdy/rdy) rats were separ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(86)80006-9
更新日期：1986-11-01 00:00:00
abstract：:Form-deprivation of chicks during early postnatal development results in ocular enlargement and great myopic refractive error (form-deprivation myopia). Previous studies have indicated that the retina, RPE and choroid play important roles in ocular enlargement in form-deprivation myopia. We aimed to isolate genes up-r...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0765
更新日期：2000-02-01 00:00:00
abstract：:The Purkinje cell degeneration (pcd) mutant mouse rapidly loses cerebellar Purkinje cells and about 50% of its retinal photoreceptor cells at between 3 and 5 weeks of age, and thereafter slowly loses the remaining photoreceptor cells during the first year of life. An ultrastructural study of the developing photorecept...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90019-o
更新日期：1992-05-01 00:00:00
abstract：:Gene therapy has been proposed as a feasible strategy for RGC survival and optic nerve regeneration. Some preclinical and clinical studies revealed intraocular inflammation after intravitreal injection of adeno-associated virus (AAV) by slit-lamp or indirect ophthalmoscope. Here we evaluate the longitudinal profile of...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.107955
更新日期：2020-04-01 00:00:00
abstract：:When aqueous humour outflow resistance is measured by two-level constant pressure perfusion in non-human primate eyes, a progressive decrease in outflow resistance, known as the 'washout effect' occurs with time. The effect of age on total outflow resistance washout (the reciprocal of outflow facility (OF)) was determ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.04.010
更新日期：2005-12-01 00:00:00
abstract：:Anatomic and physiological barriers limit drug delivery to the posterior segment of the eye via topical or systemic administration. Intravitreal administration has proven to be a safe and effective means of treating various posterior segment diseases. Elimination of a compound from the vitreous chamber may depend on l...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00311-1
更新日期：2003-03-01 00:00:00
abstract：PURPOSE:To investigate the potential protective effect of novel G protein coupled estrogen receptor (GPER1) against the neurotoxicity induced by NMDA in the mouse retina. METHODS:We induce retinal ganglion cells (RGCs) toxic injury through intravitreal injection of NMDA or acute ocular hypertension (AOH) induced by an...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107826
更新日期：2019-12-01 00:00:00
abstract：:The combined effects of adenosine and acetylcholine on the intracellular free-Ca2+ concentration in nonpigmented epithelial cells of the rabbit ciliary body were investigated using fura-2 fluorescence-ratio imaging. Acetylcholine (10 microM) by itself produced a modest increase in [Ca2+]i. Acetylcholine in combination...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0194
更新日期：1997-02-01 00:00:00
abstract：:Owing to a rapidly aging population, vision impairment caused by age-related cataract has become very common. Age-related cataract has also become one of the principal causes of blindness, and apoptosis of lens epithelial cells contributes to non-congenital cataract development. Previous studies have reported that mic...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.04.018
更新日期：2016-06-01 00:00:00
abstract：:Colchicine, a naturally occurring alkaloid, inhibits both fast and slow axoplasmic transport by prevention of microtubule assembly. We have examined the influence of colchicine on the neurogenically mediated acute inflammatory responses to topically administered nitrogen mustard and formaldehyde on the albino rabbit e...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90071-x
更新日期：1984-12-01 00:00:00
abstract：:By methods of subtraction-hybridization of lambda ZAP cDNA libraries, prepared from 4-week-old rat lens epithelial cells (capsule) and lens fiber cells (decapsulated lens), we have isolated a specific cDNA clone whose target mRNA is about 600 b long. Northern blot hybridization analysis data showed that the target mRN...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(05)80009-0
更新日期：1995-06-01 00:00:00
abstract：:An enzymatic procedure for the estimation of organic hydroperoxides has been adapted to biological tissues and applied to the measurement of hydroperoxides in the rat retina. Hydroperoxides are determined from the coupled activities of glutathione peroxidase and glutathione reductase as measured by the loss of NADPH a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90116-1
更新日期：1983-03-01 00:00:00
abstract：:Female New Zealand White rabbits were treated subcutaneously with 0.0375 or 0.15 mg kg-1 dexamethasone or physiological saline solution from the 8th through to the 11th day of gestation. Short-term administration of 0.15 mg kg-1 dexamethasone induced two types of morphological lens deviations in the offspring (lenses ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90008-3
更新日期：1984-08-01 00:00:00
abstract：:Tenon's fibroblast (TF) bleb encapsulation is a common cause of filtering surgery failure. Various pharmacologic agents have been used in an attempt to inhibit this response. The effects of three angiogenesis inhibitors were studied. AGM-1470, a fumagillin analog and hydrocortisone 21-phosphate (HC21P) complexed with ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1037
更新日期：1994-04-01 00:00:00
abstract：:Increasing evidence points to inflammation as a key factor in the pathogenesis of diabetic retinopathy (DR). Choroidal inflammatory changes in diabetes have been reported and in vivo choroidal thickness (CT) has been searched as a marker of retinopathy with contradictory results. We aimed to investigate the early stag...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108195
更新日期：2020-10-01 00:00:00
abstract：:Measurement of intraocular pressure (IOP) is an essential tool in monitoring glaucoma. Single IOP assessments during clinical routine examinations represent punctual values and are not able to identify IOP fluctuations and spikes. Telemetric IOP measurements are able to monitor IOP during the day and night, and are lo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.10.020
更新日期：2018-01-01 00:00:00
abstract：:Recent studies of the distribution and relative concentration of caveolin-1 in fractions of bovine lens epithelial and fiber cells have led to the novel concept that caveolin-1 may largely exist as a peripheral membrane protein in some cells. Caveolin-1 is typically viewed as a scaffolding protein for caveolae in plas...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.05.011
更新日期：2007-10-01 00:00:00
abstract：:We wished to conduct a light and electron microscopic investigation of pigmentation within the trabecular meshwork of normals and primary open angle glaucoma (POAG) patients. In particular we wished to get a precise determination of whether there was a relationship between pigmentation and age. In addition we wanted t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.10.009
更新日期：2006-06-01 00:00:00
abstract：:Eosinophil infiltration is one of the aspects of the inflammatory response to an allergen. The aim of this study was to establish T-helper type-2 (Th2)-induced conjunctival eosinophilia in mice to evaluate the effect of anti-allergic drugs. Th2 clone, D10.G4.1. (D10) with its specific antigen, conalbumin was co-inject...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0677
更新日期：1999-07-01 00:00:00
