Abstract：
:Inadequate cross-linking between collagen lamellae is a characteristic feature of keratoconus corneas. The formation of covalent bonds between collagen and elastin fibrils, which maintain the biomechanical properties of the cornea, is mediated by the cuproenzyme lysyl oxidase and four lysyl oxidase-like enzymes. The aim of this study was to determine the distribution of lysyl oxidase and the total lysyl oxidase activity (lysyl oxidase and the four lysyl oxidase-like enzymes) in control and keratoconic corneas. Seven control and eight keratoconic corneas were used for the imunohistochemical detection of lysyl oxidase in corneal cryosections using two different antibodies. The total lysyl oxidase activity in the culture medium of corneal fibroblasts from six explanted keratoconic and four control corneas was measured using a fluorometric assay in the presence and absence of the lysyl oxidase inhibitor beta-aminopropionitrile and determined as the production of H(2)O(2) in nM per μg of total protein. In the control tissue, the most intense signal for lysyl oxidase was present in the corneal epithelium, in which perinuclear dots brightly projecting from more or less homogenous cytoplasmic staining may represent the lysyl oxidase propeptide. Less intense staining was present in keratocytes, the extracellular matrix and in the corneal endothelium. The epithelium of the limbus and the perilimbal conjunctiva showed intense to very intense staining. The distribution of lysyl oxidase was clearly decreased in at least five of the eight keratoconic specimens. The most marked signal reduction was observed in the stromal matrix and in keratocytes. Moreover, the signal in pathological specimens revealed a more irregular pattern, including the presence of intra- and extracellular clumps in the epithelium. Interestingly, endothelial cells showed no or very weak staining in areas just beneath negative stromal tissue. The mean activity of total lysyl oxidase in the keratoconic samples (2.60 ± 2.23 nM H(2)O(2)/μg of total protein) was more than 2.5-fold lower than in control tissue (6.83 ± 2.53 nM H(2)O(2)/μg of total protein), and the decrease was statistically significant (p = 0.0178). The location of lysyl oxidase in the healthy cornea, limbus and perilimbal conjunctiva was described. We hypothesize that the restricted lysyl oxidase distribution in keratoconic corneas, and particularly the decrease of total lysyl oxidase activity in cultured keratoconic fibroblasts, is one potential reason for the inadequate collagen cross-linking that is a hallmark of this disease.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Dudakova L,Liskova P,Trojek T,Palos M,Kalasova S,Jirsova Kdoi
10.1016/j.exer.2012.09.005subject
Has Abstractpub_date
2012-11-01 00:00:00pages
74-81eissn
0014-4835issn
1096-0007pii
S0014-4835(12)00282-5journal_volume
104pub_type
杂志文章abstract：:This study tested the hypothesis that the beta-adrenergic antagonists betaxolol and timolol, cause retinal arteriolar vasodilatation in addition to their ability to reduce intraocular pressure (IOP), and compared their vasodilatory ability with that of a known Ca2+ channel entry blocker nimodipine in donor human and p...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0495
更新日期：1998-07-01 00:00:00
abstract：:Female New Zealand White rabbits were treated subcutaneously with 0.0375 or 0.15 mg kg-1 dexamethasone or physiological saline solution from the 8th through to the 11th day of gestation. Short-term administration of 0.15 mg kg-1 dexamethasone induced two types of morphological lens deviations in the offspring (lenses ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90008-3
更新日期：1984-08-01 00:00:00
abstract：:Retinoblastoma generally occurs before 5 years of age and often requires enucleation (surgical removal of one eye) of the cancerous eye. We have previously shown using behavioural methods that this disruption in binocular vision during the critical period of visual development results in impaired face perception. In t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.09.013
更新日期：2019-06-01 00:00:00
abstract：:We have examined the mercapturic acid pathway of the cataractous rabbit lens following induction by naphthalene as an oxidative foreign substance. 1,2-Naphthoquinone, which is formed in the eye from naphthalene diol and other naphthalene derivatives by a combination of enzymic and non-enzymic reactions, readily oxidiz...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(88)90058-9
更新日期：1988-09-01 00:00:00
abstract：:Early stages of glaucoma and optic neuropathies are thought to show inner retina remodeling and functional changes of retinal ganglion cells (RGCs) before they die. To assess RGC functional plasticity, we investigated the contrast-gain control properties of the pattern electroretinogram (PERG), a sensitive measure of ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.11.008
更新日期：2016-04-01 00:00:00
abstract：:The following lectins: Con A, WGA, sWGA, PNA, RCA and UEA were used to study developmental changes in the expression of glycoconjugates during chicken lens morphogenesis. Con A, WGA, sWGA binding epitopes were observed in the lens placode and vesicle. Once the fiber mass was formed, the glycoconjugates were mainly fou...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0047
更新日期：1996-04-01 00:00:00
abstract：:Preretinal pH was measured in the avascular region of 19 rabbit retinas during alternating cycles of light and dark, using a miniature, needle-type pH electrode. In 14 experiments with the rabbits breathing room air, cyclic changes in pH, decreasing in the dark and increasing in the light, were observed. The transitio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(91)90106-o
更新日期：1991-12-01 00:00:00
abstract：:The gene for microphthalmia in the homozygous cinnamon mouse produces colobomatous microphthalmia due to failure of closure of the optic fissure. Optic fissure formation and closure were examined in control cinnamon and homozygous microphthalmic foetuses from the 10th to the 13th gestational day using light and electr...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(89)90093-6
更新日期：1989-08-01 00:00:00
abstract：:There is increasing evidence that the macular pigment carotenoids, lutein and zeaxanthin, may play an important role in the prevention of age-related macular degeneration, cataract, and other blinding disorders. Although it is well known that the retina and lens are enriched in these carotenoids, relatively little is ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0954
更新日期：2001-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80010-6
更新日期：1987-02-01 00:00:00
abstract：:N-methyl-D-aspartate (NMDA)-induced excitotoxicity is an acute form of experimental retinal injury as a result of overactivation of glutamate receptors. NLRP3 (nucleotide-binding domain, leucine-rich-repeat containing family, pyrin domain containing-3) inflammasome, one of the most studied sensors of innate immunity, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.01.018
更新日期：2019-04-01 00:00:00
abstract：:Diabetic retinopathy is a sight-threatening complication of diabetes, characterized by loss of retinal pericytes and abnormal angiogenesis. We previously demonstrated that extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions are able to enter the pericytes, causing thei...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.07.003
更新日期：2018-11-01 00:00:00
abstract：:The nature and the extent of acute injury to corneal endothelial cells caused by exposure to ultrasound radiation were characterized, as well as the long-term reaction of these cells to this form of injury. It was found that the degree of lethal cell injury induced by ultrasound scaled with exposure intensity and dura...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0626
更新日期：1999-04-01 00:00:00
abstract：:Over the past 40 years, several groups have shown that epithelial debridement results in the death of keratocytes subjacent to the wound area. More recently this cell death has been shown to involve apoptosis. The purpose of this project was to examine the proliferative response of the normally quiescent keratocytes t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0926
更新日期：2001-01-01 00:00:00
abstract：:Immunocytochemistry with a monoclonal antibody against the 28-kDa calcium-binding protein (28-kDa CaBP) was used to study horizontal cells in both congenic control (RCS-rdy+) and dystrophic (RCS) 3-month-old rat retina. We found that horizontal cells in the RCS-rdy+ rat retinas were distributed evenly from the centre ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1109
更新日期：1993-08-01 00:00:00
abstract：:The passage of glucose within the cornea has been thought to occur by passive diffusion processes. However, corneal glucose concentration profiles have not been established to support this notion. While microfluorometric methods of metabolite assay typically have been used as a means of assessing regional brain metabo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(88)90037-1
更新日期：1988-11-01 00:00:00
abstract：:Angiotensin II and aldosterone are the main effectors of the renin-angiotensin aldosterone system (RAAS) and have a central role in hypertension as well as cardiovascular and renal disease. The localization of RAAS components within the retina has led to studies investigating the roles of angiotensin II, aldosterone a...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2019.107766
更新日期：2019-10-01 00:00:00
abstract：:The protein phosphatase 2B inhibitor, FK506, is an immunomodulatory polypeptide that has neuroprotective properties, the mechanisms of which have not been elucidated. A possible mechanism may be phosphorylation-mediated regulation of glutamate transporter activity. In the present study, we investigated the effect of F...
journal_title：Experimental eye research
pub_type： 信件
doi：10.1016/j.exer.2010.04.011
更新日期：2010-08-01 00:00:00
abstract：:Choroidal neovascularization (CNV) is one of the most frequent causes of severe and progressive vision loss, while its pathogenesis is still poorly understood. Focal adhesion kinase (FAK), a non-receptor tyrosine kinase, plays a crucial role in linking signals initiated by both the extracellular matrix (ECM) and solub...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.11.034
更新日期：2009-05-01 00:00:00
abstract：:The aqueous humor is produced by the epithelium of the ciliary body, a complex structure encircling the anterior segment of the eye. Aqueous humor production occurs by active transport, but the mechanism of this process is not understood. To produce a preparation in which active transport can be investigated, we have ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1156
更新日期：1993-11-01 00:00:00
abstract：:The aim of this study was to test the selectivity, in-vivo effectiveness, and potential mechanism of action of a linomide analogue (N-phenyl-1,2-dihydro-4-hydroxyl-2-oxo-quinoline-3-carboxamide, Lin05) for inhibition of choroidal neovascularization. The selectivity of Lin05 was tested in cell proliferation assays with...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.10.013
更新日期：2010-12-01 00:00:00
abstract：:The mechanical, histological and pharmacological properties of the isolated cat ophthalmociliary artery are reported and compared for passively stretched ring segments from three locations on the artery. Ring segments were mounted in a modified myograph system after the method of Hogestatt, Andersson and Edvinsson (19...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90047-v
更新日期：1992-03-01 00:00:00
abstract：:Stroboscopic luminance flicker has been found to prevent the increase in eye growth normally associated with form deprivation through the release of retinal dopamine. In this study, we sought to investigate whether dopamine plays a role in the decreased growth observed with 2 Hz sine-wave luminance flicker and increas...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107822
更新日期：2019-12-01 00:00:00
abstract：:Erythropoietin (EPO) plays a critical role in the vascular system and exhibits angiogenic activity in endothelial cells (ECs) such as stimulation of cell proliferation, migration and tube formation in vitro. EPO is the major regulator of cell proliferation and differentiation of erythroid precursors and there by preve...
journal_title：Experimental eye research
pub_type： 杂志文章,收录出版
doi：10.1016/j.exer.2010.03.005
更新日期：2010-06-01 00:00:00
abstract：:By means of X-ray diffraction analysis, structural conversions of crystallins in human lens were detected in senile cataract and upon artificial dehydration of lens tissue. In senile cataract certain characteristics of the native three-dimensional structure of gamma- and beta-crystallins are completely lost, whereas d...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(89)80044-2
更新日期：1989-11-01 00:00:00
abstract：:Traumatic optic neuropathy (TON) can occur following blunt trauma to the orbit and can lead to permanent vision loss. In this study, we investigated the effectiveness of elamipretide (MTP-131), a small mitochondrially-targeted tetrapeptide, in conjunction with etanercept, a tumor necrosis factor (TNF) inhibitor, as ne...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2020.108178
更新日期：2020-10-01 00:00:00
abstract：:Bombina variegata 8 (Bv8), also known as prokineticin-2 (PK-2), is a potent pro-angiogenic factor. However, its role in retinal neovascularization (RNV) remains unknown. In this study, we explored the role of Bv8 in the pathogenesis of RNV. We found that the expression of Bv8 was significantly increased in two differe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.107993
更新日期：2020-04-01 00:00:00
abstract：:Anatomic and physiological barriers limit drug delivery to the posterior segment of the eye via topical or systemic administration. Intravitreal administration has proven to be a safe and effective means of treating various posterior segment diseases. Elimination of a compound from the vitreous chamber may depend on l...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00311-1
更新日期：2003-03-01 00:00:00
abstract：:The purpose of this study was to evaluate specific keratocyte phenotypes (keratocyte, fibroblast, myofibroblast) for cell contractility and ability to contract extracellular matrix. Rabbit keratocyte phenotype was modulated by exposure to optimal proliferative doses of IGF-I, IL-1alpha, FGF2, PDGF-AB, and TGFbeta(1). ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00188-x
更新日期：2003-11-01 00:00:00
abstract：:Invagination of the optic vesicle to form the optic cup results in the formation of two apposed layers of neuroepithelium which follow divergent developmental pathways. Changes in the expression of cell surface molecules may be either the cause or result of important inductive signals during this process. We have used...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90088-c
更新日期：1990-11-01 00:00:00
