Abstract：
:Diabetic retinopathy is a sight-threatening complication of diabetes, characterized by loss of retinal pericytes and abnormal angiogenesis. We previously demonstrated that extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions are able to enter the pericytes, causing their detachment and migration, and stimulating angiogenesis in vitro. The purpose of this work was the molecular and functional characterization of EVs derived from diabetic subjects with or without diabetic retinopathy, compared with healthy controls. Characterization of EVs extracted from serum/plasma of diabetic patients with or without retinopathy, and healthy controls, was performed by FACS and microarray analysis of microRNA (miRNA) content. Relevant miRNA expression was validated through qRT-PCR. EV influence on pericyte detachment, angiogenesis and permeability of the blood-retinal barrier was also investigated. Diabetic subjects had a 2.5 fold higher EV concentration than controls, while expression of surface molecules was unchanged. Microarray analysis revealed 11 differentially expressed miRNAs. Three of them (miR-150-5p, miR-21-3p and miR-30b-5p) were confirmed by qRT-PCR. Plasma EVs from subjects with diabetic retinopathy induced pericyte detachment and pericyte/endothelial cell migration, increased the permeability of pericyte/endothelial cell bilayers and the formation of vessel-like structures, when compared with EVs from controls. In conclusion, circulating EVs show differences between diabetic patients and healthy subjects. EVs extracted from plasma of diabetic retinopathy patients are able to induce features of retinopathy in in vitro models of retinal microvasculature. Our data suggest a role for miR-150-5p, miR-21-3p and miR-30b-5p as potential biomarkers of the onset of diabetic retinopathy.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Mazzeo A,Beltramo E,Lopatina T,Gai C,Trento M,Porta Mdoi
10.1016/j.exer.2018.07.003subject
Has Abstractpub_date
2018-11-01 00:00:00pages
69-77eissn
0014-4835issn
1096-0007pii
S0014-4835(18)30108-8journal_volume
176pub_type
杂志文章abstract：:Rhegmatogenous retinal detachment is by far the most common indication for retinal surgery and a major cause of severe vision loss. Increased levels of glutamate found in the vitreous of human patients and persistent remodeling, even after reattachment, suggest substantial neurochemical, functional and anatomical chan...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.02.009
更新日期：2012-04-01 00:00:00
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journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1999-10-01 00:00:00
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pub_type： 杂志文章
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更新日期：2020-06-01 00:00:00
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journal_title：Experimental eye research
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90153-l
更新日期：1990-10-01 00:00:00
abstract：:Microvascular dysfunctions due to altered interactions between endothelial cells (ECs) and pericytes are key-events in the pathogenesis of diabetic retinopathy. Extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions enter pericytes, cause their detachment and migration, a...
journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：2019-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
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更新日期：2013-09-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.07.003
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journal_title：Experimental eye research
pub_type： 杂志文章
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更新日期：1995-10-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(86)80089-6
更新日期：1986-08-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.07.006
更新日期：2017-09-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90058-z
更新日期：1992-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.01.008
更新日期：2015-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.06.003
更新日期：2015-08-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80047-7
更新日期：1987-06-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.06.001
更新日期：2004-03-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.03.009
更新日期：2007-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0843
更新日期：2000-06-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.05.010
更新日期：2008-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.exer.2004.08.024
更新日期：2005-01-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2003.11.016
更新日期：2004-04-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90031-4
更新日期：1985-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108281
更新日期：2020-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90065-4
更新日期：1984-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.09.013
更新日期：2019-06-01 00:00:00
abstract：:Retinal arrestin is known to participate in the quenching of phototransduction through binding to light-activated and phosphorylated rhodopsin. Recently, a splice variant of retinal arrestin was identified in bovine photoreceptors which could bind unphosphorylated photoactive rhodopsin. In this report, a splice varian...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0069
更新日期：1996-06-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2004.05.017
更新日期：2004-10-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0422
更新日期：1998-02-01 00:00:00
abstract：:A substantial change in the glycoconjugates of the rat interphotoreceptor matrix (IPM) has recently been shown to occur between post-natal day (P) 14 and P16 in rat retinas using lectin histochemistry. This suggests that the sialic acid content on the terminus of N-glycoside linked carbohydrate chains of the IPM incre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1013
更新日期：1993-01-01 00:00:00
