Abstract：
:Fibroblast growth factors (FGF) are multipotent cytokines with demonstrated mitogenic, neurotrophic and angiogenic properties. There is evidence that they have multiple functions during and after development of the vertebrate eye. Amongst these, the role of FGF receptor mediated signaling in the retinal pigmented epithelium (RPE) is not yet well understood. FGF-2 is produced in RPE cells and may play a role in photoreceptor development and/or survival in vivo. It may also stimulate growth of melanocytes and angiogenesis in the choroid. To address these questions, we have specifically disrupted FGF signaling by generating lines of transgenic mice expressing dominant-negative FGF receptor 1 (FGFR-1) in the pigmented cells. Histological analysis of the eyes were conducted on hemizygous and homozygous mice at different ages. In homozygotes, eye growth is strongly impaired during embryogenesis leading to massive eye degeneration seen in the early post-natal stages. In hemizygotes, the choroid is thinned and the finger-like junctions between RPE cells and photoreceptors are disrupted. Scanning electron microscopy of the choroid vasculature showed that choriocapillary density, diameter and branching are strongly affected. As mice age, they develop progressive retinal degeneration as evidenced by photoreceptor cell loss. Our results are in agreement with the hypothesis that FGF signaling in the RPE participates in photoreceptor survival in vivo. Our model provides evidence that FGF signaling is also involved in choroidal angiogenesis by a process that could relate to induction of terminal branching.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Rousseau B,Dubayle D,Sennlaub F,Jeanny JC,Costet P,Bikfalvi A,Javerzat Sdoi
10.1006/exer.2000.0892keywords:
subject
Has Abstractpub_date
2000-10-01 00:00:00pages
395-404issue
4eissn
0014-4835issn
1096-0007pii
S0014-4835(00)90892-3journal_volume
71pub_type
杂志文章abstract：:Extracts of bovine corneal stroma have been shown to activate keratocytes in culture to proliferate. We fractionated stromal extract on a column of Sephacryl S-300 and tested the fractions for mitogenic activity using cell culture and for the presence of IGF-II and its binding protein IGFBP-2 by Western blot. We found...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.12.004
更新日期：2008-03-01 00:00:00
abstract：:Monkey eyes are useful for ophthalmologic research into eye diseases because their histological and functional properties are very similar to those of humans. The monkey laser-induced high intraocular pressure (IOP) model is a common model for ophthalmologic research, especially into glaucoma. Although several studies...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.02.004
更新日期：2008-05-01 00:00:00
abstract：:The ocular lens is surrounded by the lens capsule, which is an elastic and unusually thick basal membrane. Anionic sites are thought to be responsible for charge-selective permeability barriers in basal membranes. We have used cationic colloidal gold as a tracer for anionic binding sites to investigate the distributio...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0952
更新日期：2001-03-01 00:00:00
abstract：:Prolyl oligopeptidase (EC 3.4.21.26) has been purified 26,000-fold from bovine lens tissue by anion-exchange chromatography, gel filtration and isoelectric focussing, with an overall yield of 11%. The purified enzyme exhibited an isoelectric point of 4.8, a pH optimum of 7.5 and a molecular mass of 72 kDa under both n...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1086
更新日期：1994-07-01 00:00:00
abstract：:The effect of kainic acid (KA) on rat retinal morphology was investigated with emphasis on the ganglion cell layer. Two hours after intraocular injection of KA, 70% of the neurones in the ganglion cell layer display morphological alterations. These alterations at the ultrastructural level include the absence of identi...
journal_title：Experimental eye research
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doi：10.1016/0014-4835(83)90014-3
更新日期：1983-02-01 00:00:00
abstract：:We aim to determine whether lymphatic drainage from the eye changes with age. Using quantitative photoacoustic tomography, groups of young and older mice were studied in the live state. 10 CD-1 mice of 2-3 months (5M/5F) were studied in addition to 13 older mice of 12-13 months (6M/7F). In each of 23 mice, near-infrar...
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journal_title：Experimental eye research
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更新日期：1990-06-01 00:00:00
abstract：:Recent studies indicate a key role of Mitf (microphthalmia-associated transcription factor) in the differentiation of the retinal pigment epithelium (RPE). To explore transdifferentiation processes, we used small interfering RNA (siRNA) targeting Mitf. Transfection of embryonic chick RPE cells with a non-silencing flu...
journal_title：Experimental eye research
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doi：10.1016/j.exer.2005.01.004
更新日期：2005-07-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1146
更新日期：1993-10-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1016/0014-4835(91)90257-f
更新日期：1991-02-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2006.06.004
更新日期：2006-11-01 00:00:00
abstract：PURPOSE:To assess the inter-ocular and inter-session reliability for a range of parameters derived from the photopic electroretinogram (ERG) in a group of normal non-human primates. METHODS:Inter-ocular differences for photopic ERGs were assessed in a group of normal anesthetized adult rhesus monkeys (Macaca mulatta, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2003.09.013
更新日期：2004-01-01 00:00:00
abstract：:The conjunctival associated lymphoid tissue is considered to be an integral part of the mucosal immune system. Under normal circumstances immune mechanisms in mucosal associated lymphoid tissue of the gut and bronchus can selectively suppress, rather than enhance, immune responsiveness to encountered antigens, inducin...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1997.0297
更新日期：1997-06-01 00:00:00
abstract：:Changes in the distribution of keratan sulfate in the anterior segment of developing ocular tissue were investigated histochemically and immunohistochemically using human eyes from 5 weeks gestation to 2 years after birth. Enzyme digestion methods with streptomyces hyaluronidase, chondroitinase and keratanase showed t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1018
更新日期：1994-03-01 00:00:00
abstract：:The response of the lens to changes in the distribution and concentration of electrolytes can be measured by characterizing the changes in resistivity that occur secondary to variations in intracellular composition. Regional intracellular cytoplasmic resistivities of lens fiber cells can be approximated by homogenates...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80164-1
更新日期：1987-04-01 00:00:00
abstract：:Cysteine cathepsins are a family of proteases involved in intracellular protein turnover and extracellular matrix degradation. Cathepsin B (Ctsb) and cathepsin Z (Ctsz) promote tumorigenesis and Ctsb is a known modulator of tumor angiogenesis. We therefore investigated the angiomodulatory function of these cathepsins ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.06.014
更新日期：2013-10-01 00:00:00
abstract：:Aim of present investigation was to study the effect of catechin and the combination of catechin and cetirizine in ovalbumin induced animal model of allergic conjunctivitis. Guinea pigs were divided into 5 groups: normal control, disease control, disease treated with catechin 100 mg/kg, disease treated with cetirizine...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.05.020
更新日期：2015-08-01 00:00:00
abstract：:Na,K-ATPase was detected in crude microsomes of guinea-pig retinal layers, cornea and lens by immunoblotting using antibodies generated against purified kidney Na,K-ATPase. The antiserum cross-reacted with the alpha catalytic subunit of the enzyme from the eye tissues but not the beta glycoprotein subunit, suggesting ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(85)90136-8
更新日期：1985-05-01 00:00:00
abstract：:Research over the past two decades has shown that the growth of young eyes is guided by vision. If near- or far-sightedness is artificially imposed by spectacle lenses, eyes of primates and chicks compensate by changing their rate of elongation, thereby growing back to the pre-lens optical condition. Little is known a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0813
更新日期：2000-04-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.03.021
更新日期：2005-11-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1168
更新日期：1993-11-01 00:00:00
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journal_title：Experimental eye research
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doi：10.1006/exer.1996.0265
更新日期：1997-05-01 00:00:00
abstract：:The CLN2 form of neuronal ceroid lipofuscinosis is an autosomal recessively inherited lysosomal storage disease that is characterized by progressive vision loss culminating in blindness, cognitive and motor decline, neurodegeneration, and premature death. CLN2 disease results from mutations in the gene that encodes th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2015.02.012
更新日期：2015-05-01 00:00:00
abstract：:Non-invasive imaging is an invaluable diagnostic tool in ophthalmology. Two imaging devices, the scanning laser ophthalmoscope (SLO) and spectral domain optical coherence tomography (SDOCT), emerged from the clinical realm to provide research scientists with a real-time view of ocular morphology in living animals. We ...
journal_title：Experimental eye research
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doi：10.1016/j.exer.2016.10.001
更新日期：2016-12-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90015-m
更新日期：1990-01-01 00:00:00
abstract：:The lacrimal glands of male NOD mice exhibit many of the features of the human lacrimal gland in patients afflicted with the autoimmune disease, Sjögren's syndrome, including loss of secretory functions and lymphocytic infiltration into the lacrimal gland. To elucidate the early changes in the secretory pathway associ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2005.04.019
更新日期：2006-01-01 00:00:00
abstract：:To determine the effect of roscovitine, a potent antiviral in tissue culture administered intramuscularly to rabbits or by eye drops to mice for the treatment of herpetic keratitis this study was commenced.New Zealand white rabbits infected with McKrae strain herpesvirus (HSV-1) were treated twice a day with 10mg Rosc...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(03)00056-3
更新日期：2003-06-01 00:00:00
abstract：:The present study was designed to identify genomic loci modulating the susceptibility of retinal ganglion cells (RGC) to elevated intraocular pressure (IOP) in the BXD recombinant inbred mouse strain set. IOP was elevated by injecting magnetic microspheres into the anterior chamber and blocking the trabecular meshwork...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.12.013
更新日期：2018-04-01 00:00:00
abstract：:Deficient cGMP-phosphodiesterase (cGMP-PDE) activity results in elevated levels of cGMP in the rd retina before any pathological signs are observed. Since the enzyme is present in rd retina, although it is barely activated by light, we determined whether its synthesis starts at the same time as that of cGMP-PDE in nor...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(88)80026-5
更新日期：1988-03-01 00:00:00
abstract：:Uncoupling protein 2 (UCP2) is a mitochondrial transporter present in the inner membrane of mitochondria, and it uncouples substrate oxidation from ATP synthesis, thereby dissipating the membrane potential energy and consequently decreasing ATP production by mitochondrial respiratory chain. As a consequence of the unc...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.11.004
更新日期：2012-01-01 00:00:00
