Abstract：
:Retinal ischemia/reperfusion (IR) injury causes profound tissue damage, especially retinal ganglion cell death. The aims of the study were twofold: (1) to investigate the benefits of epigallocatechin-3-gallate (EGCG), the major catechin found in tea, after IR challenge, and (2) to elucidate the mechanism of EGCG inhibition of nitric oxide synthase (NOS) expression. Wistar female rats were divided into four groups: normal control, EGCG with sham operation, retinal IR, and EGCG with IR groups. EGCG (50mg/kg) was administered by intraperitoneal injection 30 min before the experiment. IR injury to a rat's retina was induced by raising intraocular pressure to 150 mmHg for 60 min. With EGCG pretreatment, retinal ganglion cell death from IR was reduced by approximately 10% 3 days afterward. EGCG significantly downregulated IR-induced glial fibrillary acidic protein expression. EGCG treatment also reduced TUNEL-positive cells after IR in the inner retina as well as IR-induced lipid peroxidation. Histological analyses showed fewer neuronal NOS and nicotinamide adenine dinucleotide phosphate diaphorase-positive cells in the retina after IR with EGCG administration. Therefore, EGCG is effective in protecting retinal ganglion cells from IR challenge by ameliorating retinal nitrosactive stress and by regulating cell death through apoptotic pathways.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Peng PH,Ko ML,Chen CFdoi
10.1016/j.exer.2008.01.008subject
Has Abstractpub_date
2008-04-01 00:00:00pages
637-46issue
4eissn
0014-4835issn
1096-0007pii
S0014-4835(08)00011-0journal_volume
86pub_type
杂志文章abstract：:Fiber cells of the ocular lens are arranged in a series of concentric shells. New growth shells are added continuously to the lens surface and, as a consequence, the preexisting shells are buried. To focus light, the refractive index of the lens cytoplasm must exceed that of the surrounding aqueous and vitreous humors...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2016.03.009
更新日期：2017-03-01 00:00:00
abstract：:Giant papillae, characteristic lesions of vernal keratoconjunctivitis, are formed as a result of the proliferation of conjunctival fibroblasts, the deposition of extracellular matrix, and the infiltration of inflammatory cells. The concentration of interleukin (IL)-4 is also increased in the tear fluid of individuals ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(02)00248-8
更新日期：2003-01-01 00:00:00
abstract：:Superoxide radicals or products generated by these radicals in a xanthine/xanthine oxidase system lyse cultured rat and human corneal epithelial cells as measured in a chromium-51 release assay. Partial protection from this lysis is afforded by superoxide dismutase and complete protection is obtained with catalase. Hy...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90004-e
更新日期：1990-01-01 00:00:00
abstract：:The purpose of our study was to establish a system for culturing normal human conjunctival epithelial (NHCE) cells under serum-free culture conditions without compromising their ability to differentiate into a mucous epithelium. To this end, small pieces of normal conjunctiva were biopsied from patients undergoing cat...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.04.005
更新日期：2007-08-01 00:00:00
abstract：:The purpose of the study was to determine histologic findings after successful laser trabeculoplasty in eyes with glaucoma. Previous studies have examined eyes in which laser did not lower intraocular pressure, or examined only the region of the laser burn itself. Nineteen autopsy eyes from 14 donors with either prima...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.07.009
更新日期：2007-10-01 00:00:00
abstract：:Prolyl oligopeptidase (EC 3.4.21.26) has been purified 26,000-fold from bovine lens tissue by anion-exchange chromatography, gel filtration and isoelectric focussing, with an overall yield of 11%. The purified enzyme exhibited an isoelectric point of 4.8, a pH optimum of 7.5 and a molecular mass of 72 kDa under both n...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1994.1086
更新日期：1994-07-01 00:00:00
abstract：:Retinal arrestin is known to participate in the quenching of phototransduction through binding to light-activated and phosphorylated rhodopsin. Recently, a splice variant of retinal arrestin was identified in bovine photoreceptors which could bind unphosphorylated photoactive rhodopsin. In this report, a splice varian...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1996.0069
更新日期：1996-06-01 00:00:00
abstract：:The nature of glucose transport at the microvascular blood-retinal barrier was studied using primary cultures of microvascular endothelial cells from bovine retina. Uptake of 3-O-methyl-D-glucose (3MG), a non-metabolizable glucose analogue, was rapid and equilibrative. 3MG uptake could be inhibited by traditional gluc...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90011-8
更新日期：1983-02-01 00:00:00
abstract：:Fibroblast growth factors (FGF) are multipotent cytokines with demonstrated mitogenic, neurotrophic and angiogenic properties. There is evidence that they have multiple functions during and after development of the vertebrate eye. Amongst these, the role of FGF receptor mediated signaling in the retinal pigmented epit...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0892
更新日期：2000-10-01 00:00:00
abstract：:Previous histochemical and biochemical studies have documented the presence of carbohydrate-containing molecules in the retinal interphotoreceptor matrix (IPM). The lectin peanut agglutinin (PNA), which preferentially binds galactose-containing carbohydrates, especially galactose-galactosamine linkages, selectively la...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80163-x
更新日期：1987-04-01 00:00:00
abstract：:Infection of the corneal endothelial cells by human cytomegalovirus (CMV) is an important cause of corneal endotheliitis. CMV endotheliitis is difficult to completely cure and relapses are frequent. This can cause blinding corneal bullous keratopathy. However, the pathogenesis of CMV endotheliitis remains undetermined...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.06.017
更新日期：2017-08-01 00:00:00
abstract：:Lactoferrin plays an important role in the defense against infections, including herpes simplex virus (HSV) keratitis. We studied the impact of three single nucleotide polymorphisms in the human lactoferrin gene on the susceptibility to HSV infections of the eye and the severity of such infections. Lactoferrin gene po...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.09.013
更新日期：2008-01-01 00:00:00
abstract：:Measurement of intraocular pressure (IOP) is an essential tool in monitoring glaucoma. Single IOP assessments during clinical routine examinations represent punctual values and are not able to identify IOP fluctuations and spikes. Telemetric IOP measurements are able to monitor IOP during the day and night, and are lo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.10.020
更新日期：2018-01-01 00:00:00
abstract：:The purpose of this study was to examine the effects of myopia-inducing and myopia recovery conditions on the scleral biomechanics of enucleated eyes of young chicks. Enucleated eyes from 5-day old chicks, with fiducial markers attached at 5 locations on the external sclera, were placed in a custom-built chamber fille...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2014.07.004
更新日期：2014-10-01 00:00:00
abstract：:The calcium-binding regulatory protein, calmodulin, was immunohistologically examined in feline rod outer segments. The peroxidase antiperoxidase technique was used for the immunohistological analysis and protein A-gold, to localize calmodulin electron-microscopically. Calmodulin was found to be located in the distal ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80178-1
更新日期：1987-03-01 00:00:00
abstract：:The early events that occur rapidly after injury trigger signal cascades that are essential for proper wound closure of corneal epithelial cells. We hypothesize that injury releases ATP, which stimulates purinergic receptors and elicits the phosphorylation of epidermal growth factor receptor (EGFR) tyrosine residues a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.03.009
更新日期：2007-07-01 00:00:00
abstract：:Cathepsin D is a ubiquitous enzyme which plays an important role in the catabolism of proteins. Enzymatic studies showed that cathepsin D is the most important lysosomal enzyme in the proteolysis of opsin. The importance of cathepsin D in the lysosomal digestion of phagocytosed photoreceptor outer segments by the reti...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0700
更新日期：1999-10-01 00:00:00
abstract：:The present study determined whether topical latanoprost, a prostaglandin (PG) F(2alpha) analog, influences the induction of anterior chamber-associated immune deviation (ACAID), corneal neovascularization (NV) or survival of corneal allografts in mice. BALB/c mice received topical latanoprost or PGE(2) once or multip...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2007.11.012
更新日期：2008-02-01 00:00:00
abstract：:Previous studies in the laboratory have shown that platelet-activating factor (PAF), a potent inflammatory mediator that accumulates rapidly in the cornea after an injury, stimulates the expression of urokinase (uPA) and matrix metalloproteinase-1 (MMP-1) and -9 (MMP-9). Tissue inhibitors of MMPs (TIMPs) and plasminog...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2001.1135
更新日期：2002-03-01 00:00:00
abstract：:The ocular lens fiber cell assembles a novel cytoskeletal element, the Beaded Filament, from CP49 and filensin, two proteins expressed only in the differentiated lens fiber cell. We report the primary sequence, secondary structural analysis, gene structure and Yeast Two Hybrid interaction data for human filensin, and ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1998.0478
更新日期：1998-05-01 00:00:00
abstract：:Previous studies have shown that corneal endothelial cells contain mRNA and protein of various growth factors. However, the role of these endogenous growth factors in corneal endothelial wound healing is not fully elucidated. In the present study, we investigated the role of endogenous factors and several growth facto...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0918
更新日期：2000-12-01 00:00:00
abstract：:Melanopsin is a non-image forming photoreceptor known to be present in the retina and it is considered to have light regulated tasks among other functions. In the present work, melanopsin presence in human lens epithelial cells as well as in human lens tissue is described for the first time. Moreover, studying the con...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2016.11.019
更新日期：2017-01-01 00:00:00
abstract：:This review is focused on iron metabolism in the retina and in the lens and its relation to their respective age-related pathologies, macular degeneration (AMD) and cataract (ARC). Several aspects of iron homeostasis are considered first in the retina and second in the lens, paying particular attention to the transpor...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2010.03.003
更新日期：2010-06-01 00:00:00
abstract：:We have investigated: (a) Phospholipid composition and phosphoinositide-PO4 turnover in rabbit cornea tissues; and (b) the effects of adrenergic and serotonergic agonists on breakdown of phosphoinositides in the rabbit cornea. The data obtained from these studies can be summarized as follows: (1) in the cornea phospha...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80047-7
更新日期：1987-06-01 00:00:00
abstract：:The effect of dietary supplementation with acetylsalicylic acid on the increased modification, and susceptibility to modification, of lens crystallins from the streptozocin diabetic rat, has been determined. This was done by the measurement of characteristic markers of protein post-translational oxidative modification...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1993.1186
更新日期：1993-12-01 00:00:00
abstract：:A micropuncture technique involving the use of microcannulas with tip diameters less than 5 microns was used to measure the pressure in Schlemm's canal and in the meshwork at distances approximately 7 and 14 microns from the inner wall of Schlemm's canal. In one set of experiments where the spontaneous intraocular pre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(92)90151-h
更新日期：1992-06-01 00:00:00
abstract：:Many ocular surface tests have been developed for the clinical diagnosis of keratoconjunctivitis sicca, and several have been used to 'validate' animal models of Sjogren's and non-Sjogren's dry eye. However, many of these tests themselves have not been systematically studied or standardized, and yet their use in anima...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2004.07.002
更新日期：2004-11-01 00:00:00
abstract：:In keratitis, keratocytes play a vital role by releasing inflammatory cytokines and expressing intercellular cell adhesion molecule-1(ICAM-1). GC31 is a peptide derived from thrombomodulin, an endogenous protein with potential anti-inflammation properties. We evaluated the protective effect of GC31 in LPS- or poly(I:C...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2017.07.017
更新日期：2017-11-01 00:00:00
abstract：:The Royal College of Surgeons (RCS) rat is an established animal model of retinitis pigmentosa, a family of inherited retinal diseases which starts with loss of peripheral vision and progresses to eventual blindness. Blood flow (BF), an important physiological parameter, is intricately coupled to metabolic function un...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2012.06.006
更新日期：2012-08-01 00:00:00
abstract：:Whole mounts of lens epithelia from rats fed on a galactose-rich diet for one, two, three or seven days were examined for morphological alterations. The meridional rows of epithelia from lenses of animals fed on galactose for seven days were found to be grossly disorganized or abnormally elongated. There was some indi...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90183-0
更新日期：1984-06-01 00:00:00
