当前位置： SCI文献检索 > EXPERIMENTAL EYE RESEARCH期刊下所有文献 > Apoptosis in the cornea: further characterization of Fas/Fas ligand system.

Apoptosis in the cornea: further characterization of Fas/Fas ligand system.

Abstract：

:This study was performed to further characterize expression and function of the Fas/Fas ligand system in the cornea. Specifically, these experiments examined (1) the effect of genetic inactivation of Fas or Fas ligand genes on keratocyte apoptosis in response to corneal epithelial wounding, (2) whether cultured human corneal epithelial and endothelial cells are competent to undergo apoptosis in response to Fas activation, (3) expression of membrane bound and soluble Fas and Fas ligand in corneal cells, and (4) the effect of IL-1 on expression of Fas and Fas ligand in corneal fibroblasts. Keratocyte apoptosis in response to corneal epithelial scrape detected by TUNEL assay and transmission electron microscopy was significantly decreased, but not eliminated, in Fas ligand -/- mice compared with control +/+ mice. There was also a decrease in Fas -/- mice that did not reach statistical significance. Thus, while the Fas/Fas ligand system is likely involved in regulating keratocyte apoptosis in response to epithelial wounding, systems with redundant function probably also modulate this response. Activation of the Fas receptor triggered death with ultrastructural changes characteristic of apoptosis in corneal epithelial and endothelial cells in culture. Since these cell types express both Fas and Fas ligand in vivo, systems must be in place to prevent uncontrolled activation via autocrine ligand-receptor interaction. Messenger RNAs coding for both membrane bound and soluble splicing variants of Fas were expressed in each corneal cell type, suggesting that soluble Fas production could be one mechanism to antagonize membrane bound Fas activation. Soluble Fas ligand protein was expressed in wounded ex vivo corneal epithelium, providing a mechanism for Fas ligand from epithelium to mediate keratocyte apoptosis. IL-1, however, also stimulated corneal fibroblasts to express Fas ligand mRNA and protein. Therefore, an alternative mode for epithelial injury to trigger keratocyte apoptosis may be by release of IL-1, subsequent induction of Fas ligand in keratocytes, and apoptosis mediated by autocrine mechanisms.

journal_name

Exp Eye Res

journal_title

Experimental eye research

authors

Mohan RR,Liang Q,Kim WJ,Helena MC,Baerveldt F,Wilson SE

doi

10.1006/exer.1997.0371

subject

Has Abstract

pub_date

1997-10-01 00:00:00

pages

575-89

issue

eissn

0014-4835

issn

1096-0007

pii

S0014-4835(97)90371-7

journal_volume

pub_type

杂志文章

在线工具

Apoptosis in the cornea: further characterization of Fas/Fas ligand system.