Abstract:
:In our earlier study we showed that invasive retinoblastoma (RB) had down regulated tetraspanin protein KAI1/CD82, a family of cell surface glycoprotein. KAI1 may link to the cell surface molecules, such as integrins, E-cadherin, and other TM4SF members, and loss of KAI1 function may have a significant role in the progression of retinoblastoma. We also showed that epithelial cell adhesion molecule (EpCAM) is overexpressed in invasive RB. EpCAM expression decreases adhesion mediated by cadherins. Thus, we were further interested in studying the role of other adhesion molecules like cadherins and catenins in RB. We studied the expression of Motility-Related Protein 1 (MRP-1)/CD9, E-cadherin, N-cadherin, alpha-catenin and beta-catenin in RB and correlated clinicopathologically in 62 archival paraffin-embedded tumors by immunohistochemistry. There were 29 tumors with no invasion of choroids/optic nerve and 33 tumors with invasion of choroid/optic nerve/orbit. Western blotting was performed on 20 tumors using the same antibodies. We observed higher expression of CD9 (P<0.001), E-cadherin (P<0.001) and alpha-catenin (P<0.001) in the non-invasive RB and higher expression of N-cadherin (P<0.001) in invasive RB. The expression of beta-catenin was not significantly different between two groups of tumors. In Western blotting, we were able to see CD9 and E-cadherin expression in a minority of tumors while N-cadherin, alpha-catenin and beta-catenin were expressed with differing intensities in a majority of tumors. Thus, invasive tumors expressed increased N-cadherin, alpha-catenin and decreased E-cadherin and CD9. Thus, it appears that loss of E-cadherin and gain of N-cadherin expression are features of invasiveness. Further functional studies are required to evaluate the role of beta-catenin in RB.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Mohan A,Nalini V,Mallikarjuna K,Jyotirmay B,Krishnakumar Sdoi
10.1016/j.exer.2006.06.014subject
Has Abstractpub_date
2007-04-01 00:00:00pages
781-9issue
4eissn
0014-4835issn
1096-0007pii
S0014-4835(07)00003-6journal_volume
84pub_type
杂志文章abstract::The facilitated diffusion glucose transporter in the plasma membrane of intact outer segments isolated from bovine retinal rods (ROS) was characterized by measurements of: (1) 14C-labeled 3-O-methylglucose fluxes; and (2) glucose-sensitive binding of 3H-labeled cytochalasin B to ROS membranes. Inhibition of 3-O-methyl...
journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
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journal_title:Experimental eye research
pub_type: 杂志文章
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journal_title:Experimental eye research
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pub_type: 杂志文章
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:1992-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2020-12-15 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章,评审
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更新日期:2017-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
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更新日期:2015-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107826
更新日期:2019-12-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.06.009
更新日期:2004-10-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0211
更新日期:1997-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2012.07.002
更新日期:2012-09-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.06.017
更新日期:2017-08-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.03.021
更新日期:2005-11-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.107933
更新日期:2020-02-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.12.015
更新日期:2014-03-01 00:00:00
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journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0528
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journal_title:Experimental eye research
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1016/j.exer.2012.02.009
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pub_type: 杂志文章
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更新日期:1995-10-01 00:00:00
abstract::The purpose of this investigation is to characterize parvalbumin-immunoreactive (IR) neurons in the inner nuclear layer (INL) of zebrafish retina through immunocytochemistry, quantitative analysis, and confocal microscopy. In the INL, parvalbumin-IR neurons were located in the inner marginal portion of the INL. On the...
journal_title:Experimental eye research
pub_type: 杂志文章
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