Abstract:
:The present study was undertaken to develop a new animal model of subretinal neovascularization that does not involve traumatic manipulation of the eye. Using this model, the mechanism of subretinal neovascularization and its penetration through Bruch's membrane, and the various factors that contribute to this process were then examined. Male Lewis rats were immunized with interphotoreceptor retinoid binding protein (IRBP) peptide R-4, and the eyes histologically examined at various times up to 45 days after immunization. On day 12 after immunization, inflammatory cells were identified primarily in the anterior segment of the eye, with scattered cells in the retina and choroid. The inflammation was most prominent on day 14, by which time many eyes showed serous retinal detachment. By day 18 the inflammation had declined in intensity, but branches of the retinal vessels were seen extending into the choroid. Examination on day 30 revealed even fewer inflammatory cells but an accumulation of retinal pigment epithelial cells and mononuclear cells was present in the subretinal space. Examination on day 45 revealed no appreciable inflammation, but typical new vessels were found in the eyes from five of the 13 rats (38%) examined at that point. Mild inflammation of the retinochoroidal tissue can induce subretinal new vessels in rats, and this model will be useful for further study of subretinal new vessel formation.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Sakamoto T,Sanui H,Ishibashi T,Kohno T,Takahira K,Inomata H,Kohen L,Ryan SJdoi
10.1006/exer.1994.1003subject
Has Abstractpub_date
1994-02-01 00:00:00pages
155-60issue
2eissn
0014-4835issn
1096-0007pii
S0014483584710037journal_volume
58pub_type
杂志文章abstract::Although primary cultures of human lens epithelial (HLE) cells provide important information concerning the role of epithelium in normal lens and cataract formation, the lack of a cell line precludes a broad range of studies on the metabolism and molecular biology of these cells. We have, therefore developed an HLE ce...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0551
更新日期:1998-11-01 00:00:00
abstract::Ischaemia/reperfusion contributes to the pathophysiological process of many retinal diseases. Previous studies have shown that retinal ischaemia/reperfusion mainly results in neuronal degeneration, including thinning of the retina, retinal ganglion cell death and reductions in electroretinography. A high-salt diet con...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107784
更新日期:2019-11-01 00:00:00
abstract::This study was conducted to evaluate the impact of varying scleral material properties on the biomechanical response of the cornea under air-puff induced deformation. Twenty pairs of human donor eyes were obtained for this study. One eye from each pair had its sclera stiffened using 4% glutaraldehyde, while the fellow...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.107904
更新日期:2020-02-01 00:00:00
abstract::The aim of this study was to investigate the developmental potential of embryonic stem (ES) cell-derived eye-like structures as a replacement cell therapy model for retinas with N-methyl-d-aspartate (NMDA)-induced damage. For this purpose mouse ES cells were induced to differentiate into eye-like structures in vitro f...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2007.01.007
更新日期:2007-05-01 00:00:00
abstract::The purpose of this study was to evaluate specific keratocyte phenotypes (keratocyte, fibroblast, myofibroblast) for cell contractility and ability to contract extracellular matrix. Rabbit keratocyte phenotype was modulated by exposure to optimal proliferative doses of IGF-I, IL-1alpha, FGF2, PDGF-AB, and TGFbeta(1). ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(03)00188-x
更新日期:2003-11-01 00:00:00
abstract::Several studies have suggested that antioxidants retard the process of cataractogenesis by scavenging free oxygen radicals. The present study sought to assess the efficacy of the antioxidant acetyl-L-carnitine (ALCAR) in preventing selenite-induced cataractogenesis in an experimental setting. The first, in vitro phase...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.07.009
更新日期:2006-12-01 00:00:00
abstract::In our earlier study we showed that invasive retinoblastoma (RB) had down regulated tetraspanin protein KAI1/CD82, a family of cell surface glycoprotein. KAI1 may link to the cell surface molecules, such as integrins, E-cadherin, and other TM4SF members, and loss of KAI1 function may have a significant role in the pro...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.06.014
更新日期:2007-04-01 00:00:00
abstract::Transforming growth factor β (TGFβ) signaling is one of the most important signaling pathways regulating cell behavior in ocular tissues. Its functions are mainly linked to tissue fibrosis and inflammatory responses in ophthalmology. In epithelial cells, however, the growth inhibitory activity of TGFβ was reported in ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2019.03.014
更新日期:2019-05-01 00:00:00
abstract::Tenon's fibroblast (TF) bleb encapsulation is a common cause of filtering surgery failure. Various pharmacologic agents have been used in an attempt to inhibit this response. The effects of three angiogenesis inhibitors were studied. AGM-1470, a fumagillin analog and hydrocortisone 21-phosphate (HC21P) complexed with ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1994.1037
更新日期:1994-04-01 00:00:00
abstract::Na,K-ATPase was detected in crude microsomes of guinea-pig retinal layers, cornea and lens by immunoblotting using antibodies generated against purified kidney Na,K-ATPase. The antiserum cross-reacted with the alpha catalytic subunit of the enzyme from the eye tissues but not the beta glycoprotein subunit, suggesting ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(85)90136-8
更新日期:1985-05-01 00:00:00
abstract::The ability of interphotoreceptor retinoid-binding protein (IRBP) to facilitate the incorporation of retinol into retinyl esters by the retinal pigment epithelium (RPE) was examined in toad (Bufo marinus) eyecup preparations devoid of neural retina (RPE-eyecup). Solutions containing purified bovine IRBP and all-trans[...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(89)80059-4
更新日期:1989-10-01 00:00:00
abstract::Rituximab is a monoclonal antibody directed against the CD20 B-cell antigen and is approved for the treatment of B-cell lymphoma. We investigated the pharmacokinetics of rituximab following intravitreal administration to assess the feasibility of treating primary intraocular lymphoma. Intravitreal injections of rituxi...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.09.018
更新日期:2006-05-01 00:00:00
abstract::Recent studies have shown that lactate coupled water flux is the underlying mechanism of the corneal endothelial pump, which is highly dependent on the presence of bicarbonate. In this study we test the hypothesis that the increased intracellular pH (pHi) caused by bicarbonate stimulates glycolytic activity and the pr...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2020.108193
更新日期:2020-10-01 00:00:00
abstract::The lacrimal glands of male NOD mice exhibit many of the features of the human lacrimal gland in patients afflicted with the autoimmune disease, Sjögren's syndrome, including loss of secretory functions and lymphocytic infiltration into the lacrimal gland. To elucidate the early changes in the secretory pathway associ...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2005.04.019
更新日期:2006-01-01 00:00:00
abstract::In keratitis, keratocytes play a vital role by releasing inflammatory cytokines and expressing intercellular cell adhesion molecule-1(ICAM-1). GC31 is a peptide derived from thrombomodulin, an endogenous protein with potential anti-inflammation properties. We evaluated the protective effect of GC31 in LPS- or poly(I:C...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2017.07.017
更新日期:2017-11-01 00:00:00
abstract::Eleven monoclonal antibodies against different segments of bovine rhodopsin were used with immunogold-dextran markers to label Lowicryl thin sections of bovine and frog retinal photoreceptor cells for visualization by transmission electron microscopy. Antibodies against the C-terminus, F1-F2 loop and N-terminus of rho...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(86)90017-5
更新日期:1986-01-01 00:00:00
abstract::Homocysteine is an amino acid required for the metabolism of methionine. Excess homocysteine is implicated in cardiovascular and neurological disease and new data suggest a role in various retinopathies. Mice lacking cystathionine-beta-synthase (cbs(-/-)) have an excess of retinal homocysteine and develop anatomical a...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2011.12.011
更新日期:2012-03-01 00:00:00
abstract::The hyaluronan content during wound healing following excimer laser photoablation was investigated. Rabbit corneas were photoablated (Summit Omnimed, 193 nm, 5 mm diameter, 50 microns and 100 microns depth). Central optical zones of photoablated and contralateral corneas were removed 7, 30 and 90 days after surgery. C...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1996.0212
更新日期:1997-03-01 00:00:00
abstract::Retinitis Pigmentosa (RP) is an inherited disorder that may lead to blindness. In the rhodopsin S334ter-line-3 rat model of RP, the death of rods induces spatial rearrangement of cones into regular ring mosaics. Using this model, we discovered that the ring mosaics are restored to a homogeneous distribution upon appli...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.08.005
更新日期:2015-11-01 00:00:00
abstract:PURPOSE:The feasibility of corneal reconstruction with cultured adult human corneal endothelial cells (HCEC) was examined in a nude rat model. METHODS:Endothelial cells were removed from the corneas of Lewis rats using a sterile cotton swab. Cultured adult HCEC labelled with a fluorescent marker chloromethyl-benzamido...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2004.05.001
更新日期:2004-08-01 00:00:00
abstract::The distribution of collagen degradation and synthesis (turnover) between the central and peripheral areas of clear corneal allografts was determined, following transplantation of 6-mm [3H]proline-labelled corneal buttons from extensively pre-labelled, young rabbits into 13 nonradioactive mature rabbits. Control, cont...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(87)80076-3
更新日期:1987-07-01 00:00:00
abstract::Cataract is a multifactorial disease, and a large variety of stressors induce cataracts. Many cataractogenic stressors and endoplasmic reticulum (ER) stressors induce the unfolded protein response (UPR) in various cell types. The UPR is known to produce reactive oxygen species (ROS) prior to the inducement of apoptosi...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2006.01.033
更新日期:2006-09-01 00:00:00
abstract::The binding of monoclonal and polyclonal antibodies to glial fibrillary acidic protein (GFAP) antigenic sites in the rabbit retina was shown to be sensitive to aldehyde fixation. In chemically unfixed retina, the polyclonal anti-GFAP labeled Müller cells, astrocytes, and unidentified profiles in the outer plexiform la...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(90)90139-l
更新日期:1990-04-01 00:00:00
abstract::Aim of present investigation was to study the effect of catechin and the combination of catechin and cetirizine in ovalbumin induced animal model of allergic conjunctivitis. Guinea pigs were divided into 5 groups: normal control, disease control, disease treated with catechin 100 mg/kg, disease treated with cetirizine...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2015.05.020
更新日期:2015-08-01 00:00:00
abstract::Utilizing a human beta-actin promoter, a catalase cDNA expression vector was constructed. This construct was used to transfect two immortal cell lines, mouse alpha TN4-1 and rabbit N/N 1003A. The catalase activity was increased about 3.4 fold in the alpha TN4-1 cells and 38 fold in the N/N 1003A cells. Some changes in...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1998.0560
更新日期:1998-12-01 00:00:00
abstract::Adenosine, produced from the decomposition of adenosine triphosphate, is believed to provide protective effects during ischemia. On the other hand, adenosine metabolites may serve as precursors for oxygen free radical formation. The time course of formation of adenosine and its purine metabolites was studied during re...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1006/exer.1997.0391
更新日期:1997-12-01 00:00:00
abstract::We sought to characterize the ocular pharmacology, tolerability and intraocular pressure (IOP)-lowering efficacy of FR-190997, a non-peptidic bradykinin (BK) B2-receptor agonist. FR-190997 possessed a relatively high receptor binding affinity (Ki = 27 nM) and a high in vitro potency (EC50 = 18.3 ± 4.4 nM) for inositol...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2014.10.008
更新日期:2014-11-01 00:00:00
abstract::Autoradiographic studies showed selective accumulation of [3H]-glycine, [3H]-GABA, and the GABA agonist [3H]-muscimol by neurons of the mudpuppy retina. [3H]-Glycine was taken up by bipolar cells, amacrine cells, and displaced amacrine or ganglion cells. Both [3H]-GABA and [3H]-muscimol were also accumulated by bipola...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/0014-4835(84)90116-7
更新日期:1984-07-01 00:00:00
abstract::Accurate thermal models for the cornea of the eye support the development of thermal techniques for reshaping the cornea and other scientific purposes. Heat transfer in the cornea must be quantified accurately so that a thermal treatment does not destroy the endothelial layer, which cannot regenerate, and yet is respo...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/j.exer.2013.07.029
更新日期:2013-10-01 00:00:00
abstract::The purpose of this study was to determine whether trans-scleral pressure affects scleral solute permeability by altering scleral thickness or hydration, and to investigate the sustained release delivery of dexamethasone. Scleral sections from donor human globes were mounted for in vitro flux studies. Scleral thicknes...
journal_title:Experimental eye research
pub_type: 杂志文章
doi:10.1016/s0014-4835(03)00211-2
更新日期:2004-03-01 00:00:00