Isolation, culture and characterization of bovine choriocapillary endothelial cells.

abstract：:An attachment to (Haag-Streit and Topcon) slit-lamps is described. This permits projection of a patterned target into the eye and the visual or photographic analysis of the details of the image reflected from the posterior surface of the crystalline lens. A numerical assessment can then be made of the optical quality ...

journal_title：Experimental eye research

authors： Weale R

更新日期：1992-09-01 00:00:00

abstract：:The scleral spur in 37 human (age 17-87 years) and six cynomolgus monkey eyes (2-4 years) was investigated. Serial meridional and tangential sections were studied with ultrastructural and immunocytochemical methods. The bundles of the ciliary muscle do not enter the scleral spur, but their tendons, which consist of el...

journal_title：Experimental eye research

authors： Tamm E,Flügel C,Stefani FH,Rohen JW

更新日期：1992-04-01 00:00:00

abstract：:Corneal autofluorescence, as measured with a commercial scanning fluorophotometer (lambda(exc): 415-491 nm; lambda(em): 515-630 nm), is increased in patients with diabetes mellitus. However, such fluorophotometers register an average fluorescence signal over all corneal layers as a consequence of their limited axial r...

journal_title：Experimental eye research

authors： Van Schaik HJ,Coppens J,Van den Berg TJ,Van Best JA

更新日期：1999-11-01 00:00:00

abstract：:A newly-developed fibre-optic sensor (wavelength of 670 nm) was used to measure the index gradient in 16 human lenses ranging in age from 38 to 78 years. Two of these lenses had nuclear cataract. The refractive index, calculated from the proportion of reflected light at the sensor/sample interface, appeared to increas...

journal_title：Experimental eye research

authors： Pierscionek BK

更新日期：1995-04-01 00:00:00

abstract：:With the combined purpose of facilitating useful vision over a lifetime, a number of ocular cells have evolved specialized features not found elsewhere in the body. The trabecular meshwork (TM) cell at the irido-corneal angle, which is a key regulator of intraocular pressure, is no exception. Examination of cells in c...

journal_title：Experimental eye research

authors： Stamer WD,Clark AF

更新日期：2017-05-01 00:00:00

abstract：:We describe a model of acute intraocular pressure (IOP) elevation in the mouse eye that induces reversible loss of inner retinal function associated with oxidative stress, glial cell activation and minimal loss of retinal ganglion cell (RGC) number. Young healthy mouse eyes recover inner retinal function within 7-days...

journal_title：Experimental eye research

authors： Crowston JG,Kong YX,Trounce IA,Dang TM,Fahy ET,Bui BV,Morrison JC,Chrysostomou V

更新日期：2015-12-01 00:00:00

abstract：:The C-terminal region of peripherin/rds contains three predicted alpha-helical domains. One of these domains, corresponding to amino acids 311-322, form an amphiphilic alpha-helix previously shown to promote membrane fusion. The present studies were conducted to determine how the additional alpha-helical regions of th...

journal_title：Experimental eye research

authors： Muller-Weeks S,Boesze-Battaglia K,Fitzgerald C

更新日期：2002-08-01 00:00:00

abstract：:The levels of alpha 1-proteinase inhibitor (alpha 1-antitrypsin) in keratoconus, normal human, and other diseased corneas were examined. Using an immunoperoxidase technique, the presence of this inhibitor was demonstrated in the epithelium, stroma and endothelium of all corneal sections. Compared with normal human con...

journal_title：Experimental eye research

authors： Sawaguchi S,Twining SS,Yue BY,Wilson PM,Sugar J,Chan SK

更新日期：1990-05-01 00:00:00

abstract：:Dietary restriction can effectively extend lifespan and retard many age-related debilities. One hypothesis to explain the beneficial effects of dietary restriction is that it prolongs maintenance of cellular homeostasis by limiting endogenous oxidative stress and preserves oxidative defense mechanisms during aging. As...

journal_title：Experimental eye research

authors： Taylor A,Jahngen-Hodge J,Smith DE,Palmer VJ,Dallal GE,Lipman RD,Padhye N,Frei B

更新日期：1995-07-01 00:00:00

abstract：:The response of the poikilothermal lens to various incubation temperatures in vitro was compared with that of the homothermal lens. The rainbow trout lens was used as the poikilothermal lens and the rat lens as the homothermal lens. In contrast to rat lenses, cataract developed at 37 degrees C in rainbow trout lenses,...

journal_title：Experimental eye research

authors： Iwata S,Hikida M

更新日期：1985-08-01 00:00:00

abstract：:Human limbal epithelial cells (LECs) intended for treatment of limbal stem cell deficiency are commonly cultivated on a 3T3 feeder layer with complex culture medium supplemented with fetal bovine serum (FBS). However, FBS is a xenogeneic component containing poorly characterised constituents and exhibits quantitative ...

journal_title：Experimental eye research

authors： Brejchova K,Trosan P,Studeny P,Skalicka P,Utheim TP,Bednar J,Jirsova K

更新日期：2018-11-01 00:00:00

abstract：:To facilitate the peeling of internal limiting membrane or epiretinal membrane in vitreoretinal surgery, trypan blue (TB) and indocyanine green (ICG) have been used. However, the cytotoxicity of these dyes have been concerned. The aim of this study was to investigate the neurotoxic effects of TB on rat retinal ganglio...

journal_title：Experimental eye research

authors： Jin Y,Uchida S,Yanagi Y,Aihara M,Araie M

更新日期：2005-10-01 00:00:00

abstract：:Previous studies have shown that retinal damage induced by damaging light exposure is more severe in superior retina than inferior retina when measured along the vertical meridian of eyes. However, the extent of retinal damage over all retinal regions is not clear. Albino rats were exposed to bright light (5000 lux fo...

journal_title：Experimental eye research

authors： Tanito M,Kaidzu S,Ohira A,Anderson RE

更新日期：2008-09-01 00:00:00

abstract：:Primary cultures of chick lens annular pad (CLAP) cells were exposed to beta-adrenergic drugs and lipid soluble cAMP analogues. In dose-dependent manners, these agents inhibited re-entry into the cell cycle when these normally post-mitotic cells were cultured in the presence of serum. The inhibition of cell division w...

journal_title：Experimental eye research

authors： Ireland ME,Tran K,Mrock L

更新日期：1993-09-01 00:00:00

abstract：:The purpose of this study was to evaluate specific keratocyte phenotypes (keratocyte, fibroblast, myofibroblast) for cell contractility and ability to contract extracellular matrix. Rabbit keratocyte phenotype was modulated by exposure to optimal proliferative doses of IGF-I, IL-1alpha, FGF2, PDGF-AB, and TGFbeta(1). ...

journal_title：Experimental eye research

authors： Jester JV,Ho-Chang J

更新日期：2003-11-01 00:00:00

abstract：:In addition to its role as an inhibitor of urokinase plasminogen activator (uPA), plasminogen activator inhibitor-1 (PAI-1) is hypothesized to regulate epithelial cell adhesion and migration. We have previously reported that PAI-1 may be an important regulatory factor of the uPA system in cornea. The purpose of this s...

journal_title：Experimental eye research

authors： Wang Z,Sosne G,Kurpakus-Wheater M

更新日期：2005-01-01 00:00:00

abstract：:The aim of this study was to examine the murine subbasal nerve fibre plexus (SNP) regeneration altered by surgical dissection. Investigations in the mouse model addressed the regeneration capabilities of the SNP, and the influence of local ciliary neurotrophic factor (CNTF) application on the regeneration process. In ...

journal_title：Experimental eye research

authors： Reichard M,Hovakimyan M,Guthoff RF,Stachs O

更新日期：2014-03-01 00:00:00

abstract：:Following the identification of potent, orally active inhibitors of aldose reductase, the next challenge comes in demonstrating clinical utility in the treatment of diabetic complications. It is not feasible to conduct pivotal studies in all indications within the same trial and neuropathy evolved as the lead indicati...

journal_title：Experimental eye research

authors： Stribling D

更新日期：1990-06-01 00:00:00

abstract：:Immunocytochemistry with a monoclonal antibody against the 28-kDa calcium-binding protein (28-kDa CaBP) was used to study horizontal cells in both congenic control (RCS-rdy+) and dystrophic (RCS) 3-month-old rat retina. We found that horizontal cells in the RCS-rdy+ rat retinas were distributed evenly from the centre ...

journal_title：Experimental eye research

authors： Chu Y,Humphrey MF,Constable IJ

更新日期：1993-08-01 00:00:00

abstract：:Our previous gene expression analysis suggested that conjunctival epithelial cells of Sjögren's syndrome (SS) are inclined to hyper-proliferation and keratinisation status. The goal of this study is to elucidate whether such pathological situations really exist in the conjunctival epithelium of SS. Also, involvement o...

journal_title：Experimental eye research

authors： Hirai N,Kawasaki S,Tanioka H,Connon CJ,Yamasaki K,Yokoi N,Komuro A,Kinoshita S

更新日期：2006-03-01 00:00:00

abstract：:Myofibroblasts expressing α-smooth muscle actin (α-SMA) are the key cellular mediator of fibrosis. Fibrovascular epiretinal membranes from patients with proliferative diabetic retinopathy (PDR) are characterized by the accumulation of a large number of myofibroblasts. We explored the hypothesis that proliferating endo...

journal_title：Experimental eye research

authors： Abu El-Asrar AM,De Hertogh G,van den Eynde K,Alam K,Van Raemdonck K,Opdenakker G,Van Damme J,Geboes K,Struyf S

更新日期：2015-03-01 00:00:00

abstract：:A perifusion system has been designed for metabolic nuclear magnetic resonance (NMR) spectroscopy studies of the bovine retina. This system, which allows the use of unmodified commercial probes for multinuclear studies, is shown here to maintain the perifused retina in a metabolic steady state at 25 degrees C, as dedu...

journal_title：Experimental eye research

authors： Cabañas ME,Alonso J,Arús C,Anderson ME,Westler WM,Markley JL

更新日期：1993-12-01 00:00:00

abstract：:Tissue transglutaminase (tTG) is a Ca(2+)-dependent enzyme catalyzing the formation of covalent crosslinks between peptide-bound glutamine and lysine residues. Lens crystallins, including alphaB-crystallin and several beta-crystallins, are in vitro substrates for tTG. In both human and bovine fetal lens extracts treat...

journal_title：Experimental eye research

authors： Boros S,Wilmarth PA,Kamps B,de Jong WW,Bloemendal H,Lampi K,Boelens WC

更新日期：2008-02-01 00:00:00

abstract：:An interaction between the C-terminus of aquaporin-0 (AQP0) and lens beaded filament protein filensin has been reported previously; however, the region of filensin that is involved in the interaction has not been determined. This study is designed to identify the region of filensin that interacts with AQP0. Chemical c...

journal_title：Experimental eye research

authors： Wang Z,Schey KL

更新日期：2017-06-01 00:00:00

abstract：:Retinal ischemia/reperfusion (IR) injury causes profound tissue damage, especially retinal ganglion cell death. The aims of the study were twofold: (1) to investigate the benefits of epigallocatechin-3-gallate (EGCG), the major catechin found in tea, after IR challenge, and (2) to elucidate the mechanism of EGCG inhib...

journal_title：Experimental eye research

authors： Peng PH,Ko ML,Chen CF

更新日期：2008-04-01 00:00:00

abstract：:The goal of this study was to investigate the efflux of K(+) from human corneal limbal epithelial cells (HCLE) exposed to ambient levels of UVB, which is known to cause apoptosis, and to examine the effect of K(+) channel blockers on loss of potassium induced by UVB. HCLE cells were exposed to 100-200 mJ/cm(2) UVB, fo...

journal_title：Experimental eye research

authors： Ubels JL,Van Dyken RE,Louters JR,Schotanus MP,Haarsma LD

更新日期：2011-05-01 00:00:00

abstract：:Endocannabinoid system involves in neuroprotective effects on the central neural system. The cannabinoid receptor 1 (CB1R) is widely expressed in the mouse retina. However, the role of cannabinoid receptors in the retina remains unclear. In this work, we established a photoreceptor degeneration mouse model via N-methy...

journal_title：Experimental eye research

authors： Chen Y,Luo X,Liu S,Shen Y

更新日期：2018-02-01 00:00:00

abstract：:Gene therapy has been proposed as a feasible strategy for RGC survival and optic nerve regeneration. Some preclinical and clinical studies revealed intraocular inflammation after intravitreal injection of adeno-associated virus (AAV) by slit-lamp or indirect ophthalmoscope. Here we evaluate the longitudinal profile of...

journal_title：Experimental eye research

authors： Liu YF,Huang S,Ng TK,Liang JJ,Xu Y,Chen SL,Xu C,Zhang M,Pang CP,Cen LP

更新日期：2020-04-01 00:00:00

abstract：:Purpose of the study was to develop and assess a novel controlled drug delivery system of latanoprost acid (LA). Poly(lactide)/Monomethoxy-poly(ethyleneglycol) (PLA-PEG) nanoparticles (NPs) were prepared using an emulsification-solvent evaporation technique. NPs were characterized in vitro according to their size, ζ-p...

journal_title：Experimental eye research

authors： Giarmoukakis A,Labiris G,Sideroudi H,Tsimali Z,Koutsospyrou N,Avgoustakis K,Kozobolis V

更新日期：2013-07-01 00:00:00

abstract：:Mammalian lens development involves cell-cell and cell-ECM interactions. As integrins are a major family of cell adhesion molecules, we examined the expression patterns of several integrin subunits (alpha3A, alpha3B, alpha6A, alpha6B, beta1 and beta4) during rat lens development. RT-PCR, in situ hybridisation, immunof...

journal_title：Experimental eye research

authors： Wederell ED,Brown H,O'connor M,Chamberlain CG,McAvoy JW,de Iongh RU

更新日期：2005-09-01 00:00:00