Abstract：
:Human limbal epithelial cells (LECs) intended for treatment of limbal stem cell deficiency are commonly cultivated on a 3T3 feeder layer with complex culture medium supplemented with fetal bovine serum (FBS). However, FBS is a xenogeneic component containing poorly characterised constituents and exhibits quantitative and qualitative lot-to-lot variations. Human limbal explants were plated on untreated or fibrin coated plastic plates and cultured in two non-xenogeneic media (supplemented with either human serum or platelet lysate only). Our aim was to find out whether the characteristics of harvested LEC cultures are comparable to those of LEC cultivated in the gold standard - FBS-supplemented complex medium. The growth kinetics, cell proliferation, differentiation, stemness maintenance, apoptosis and contamination by other cell types were evaluated and compared among these conditions. In all of them LECs were successfully cultivated. Stemness was preserved in both xeno-free media. However, cells cultured with human serum on the fibrin-coated plates had the highest growth rate and cell proliferation and very low fibroblast-like cell contamination. These data suggest that xeno-free cell culture conditions can replace the traditional FBS-supplemented medium and thereby provide a safer protocol for ex vivo cultured limbal stem cell transplants.
journal_name
Exp Eye Resjournal_title
Experimental eye researchauthors
Brejchova K,Trosan P,Studeny P,Skalicka P,Utheim TP,Bednar J,Jirsova Kdoi
10.1016/j.exer.2018.06.019subject
Has Abstractpub_date
2018-11-01 00:00:00pages
20-28eissn
0014-4835issn
1096-0007pii
S0014-4835(18)30184-2journal_volume
176pub_type
杂志文章abstract：:Over the past 40 years, several groups have shown that epithelial debridement results in the death of keratocytes subjacent to the wound area. More recently this cell death has been shown to involve apoptosis. The purpose of this project was to examine the proliferative response of the normally quiescent keratocytes t...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2000.0926
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abstract：:Transmural electrical properties of the isolated rabbit iris-ciliary body (I-CB) were measured in Ussing-Zerahn-type chambers. Control p.d. across the preparation was -1.2 +/- 0.1 mV, with the ciliary process (aqueous)-side consistently negative with respect to the ciliary body (blood)-side and the short-circuit curre...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90096-4
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abstract：:Although it is important to investigate the effects of normal aging on the ocular circulation, few studies have examined the effects of aging on the retinal microcirculation in healthy subjects. The purpose of this study was to evaluate the effects of aging on the retinal microcirculation in healthy men. Forty-five he...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.07.019
更新日期：2009-12-01 00:00:00
abstract：:Axial-length and refraction measurements are presented for the lid-sutured rhesus monkey at 6-month intervals during the first 1.5 years of life. The experimental data are fitted to an exponential growth theory allowing the calculation of time constants, apparent time origins, and correlation coefficients for 10 eyes ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(86)90043-6
更新日期：1986-06-01 00:00:00
abstract：:Mongolian gerbils were enucleated, and a crystal of HRP placed on the cut surface of the transected optic nerves emerging from the eyeballs. After incubation in an oxygenated medium, glutaraldehyde fixation, cryo-sectioning and reaction of the sections for peroxidase activity, HRP-labelled fibres were observed in the ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80093-3
更新日期：1987-12-01 00:00:00
abstract：:Maintenance of cellular calcium levels is critical to cell function. Loss of calcium homeostasis might be a contributing factor to the development of cataract in the lens. In lens epithelium, calcium is involved in cell signaling and its precise regulation is vital. In this study, we investigated the regulation of sar...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.2002.2049
更新日期：2002-11-01 00:00:00
abstract：:Interphotoreceptor retinoid-binding protein (IRBP) was studied using immunochemical and immunocytochemical techniques in retinae of mice with allelic combinations at the rd and rds loci at different stages of development and degeneration. Until postnatal day 7 (P7), IRBP is located intracellularly in developing retina...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(88)90012-7
更新日期：1988-08-01 00:00:00
abstract：:Bombina variegata 8 (Bv8), also known as prokineticin-2 (PK-2), is a potent pro-angiogenic factor. However, its role in retinal neovascularization (RNV) remains unknown. In this study, we explored the role of Bv8 in the pathogenesis of RNV. We found that the expression of Bv8 was significantly increased in two differe...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.107993
更新日期：2020-04-01 00:00:00
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journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(84)90116-7
更新日期：1984-07-01 00:00:00
abstract：:Glutamate is an excitatory neurotransmitter in the central and peripheral nervous systems of the vertebrate. The previous studies show the presence of mRNAs of AMPA-type glutamate receptors, GluR1 and GluR2, in the optic tectum of the pigeon, suggesting glutamatergic input from the retina. The present study examined l...
journal_title：Experimental eye research
pub_type： 信件
doi：10.1016/j.exer.2009.03.024
更新日期：2009-09-01 00:00:00
abstract：:Understanding mechanisms of glaucomatous optic nerve damage is essential for developing effective therapies to augment conventional pressure-lowering treatments. This requires that we understand not only the physical forces in play, but the cellular responses that translate these forces into axonal injury. The former ...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2010.08.005
更新日期：2011-08-01 00:00:00
abstract：:Nitric oxide (NO) production by vascular endothelium is important in regulation of blood flow. Reduced production of NO can adversely affect blood flow and other vascular functions. We investigated the expression of three nitric oxide synthase (NOS) isoforms in retina and choroid of aged human eyes and eyes with AMD. ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2009.10.004
更新日期：2010-01-01 00:00:00
abstract：:The presence of the alpha(1)-adrenoceptor subtypes in various parts of the pig and rabbit eyes was investigated using [(3)H]-prazosin radioligand binding. The characterization of the subtypes was achieved by performing competition experiments with various subtype selective drugs. In the pig retina, both alpha(1A)- and...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1006/exer.1999.0753
更新日期：2000-01-01 00:00:00
abstract：:The study aimed to determine the variation in thickness and biomechanical properties between the different regions of the human sclera. Thickness measurements were carried out along eight meridian lines extending from the posterior pole to limbus in 36 human donor scleras aged 52-96 years. Strip specimens were extract...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2010.02.010
更新日期：2010-05-01 00:00:00
abstract：:Accurate thermal models for the cornea of the eye support the development of thermal techniques for reshaping the cornea and other scientific purposes. Heat transfer in the cornea must be quantified accurately so that a thermal treatment does not destroy the endothelial layer, which cannot regenerate, and yet is respo...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.07.029
更新日期：2013-10-01 00:00:00
abstract：:A lesion in cGMP metabolism has been hypothesized to cause retinal degeneration in rd mice. Available cloned cDNAs coding for proteins involved in the cGMP cascade have been used to compare the corresponding retinal RNAs in the degenerative (rd/rd) mouse at 8-11 days with those in the 8-11-day-old morphologically norm...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/s0014-4835(87)80058-1
更新日期：1987-10-01 00:00:00
abstract：:Diabetic retinopathy is a sight-threatening complication of diabetes, characterized by loss of retinal pericytes and abnormal angiogenesis. We previously demonstrated that extracellular vesicles (EVs) derived from mesenchymal stem cells cultured in diabetic-like conditions are able to enter the pericytes, causing thei...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2018.07.003
更新日期：2018-11-01 00:00:00
abstract：:Exposure of the rabbit lens, in vitro, to 10(-3) M-hydrogen peroxide resulted in an increase of the lens sodium, calcium and water content together with a decrease of the lens potassium content. No such changes were observed in the presence of 10(-4) or 10(-5) M-hydrogen peroxide. 10(-3) M-hydrogen peroxide was observ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(83)90148-3
更新日期：1983-07-01 00:00:00
abstract：:Careful control of iron availability in the retina is central to maintenance of iron homeostasis, as its imbalance is associated with oxidative stress and the progression of several retinopathies. Ferritin, known for its role in iron storage and detoxification, has also been proposed as an iron-transporter protein, th...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2020.108292
更新日期：2021-01-01 00:00:00
abstract：:Multiple species of mucins are synthesized and secreted by corneal and conjunctival epithelial cells. These mucins are vital components of the tear film protecting the ocular surface from the external environment by providing a physical and chemical barrier. The release of mucins must be tightly regulated as both muci...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2003.10.005
更新日期：2004-02-01 00:00:00
abstract：:Cochlin deposits were observed in the trabecular meshwork (TM) of 8-month-old glaucomatous DBA/2J mice, coincident with the reported onset of increased intraocular pressure and optic nerve damage. An age-dependent increase in cochlin was observed up to 10 months of age and was paralleled by a decrease in type II colla...
journal_title：Experimental eye research
pub_type： 信件
doi：10.1016/j.exer.2005.01.028
更新日期：2005-05-01 00:00:00
abstract：:The supply of L-carnosine, a bioactive dipeptide of β-alanine and l-histidine, to the retina across the blood-retinal barrier (BRB) was studied. The in vivo and in vitro studies revealed low uptake activities for [(3)H]Gly-Sar, a representative dipeptide, suggesting that l-carnosine transport plays only a minor role a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.06.002
更新日期：2013-08-01 00:00:00
abstract：:TGFβ2 induces extracellular matrix (ECM) remodeling and alters the cytoskeleton by both the canonical Smad and non-canonical signaling pathways. TGFβ2 regulates the expression of ECM proteins in trabecular meshwork (TM) cells, increases intraocular pressure (IOP) in an ex vivo perfusion organ culture model, and induce...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2013.10.017
更新日期：2013-11-01 00:00:00
abstract：:Corneal endothelium appears to be an important target tissue for retinoids and epidermal growth factor (EGF). We report here that retinoic acid, or its synthetic analogue CBS-211 A (10(-8)-10(-7) M) dose-dependently enhances the mitogenic effect of EGF on cultured bovine corneal endothelial cells (BCEC). Furthermore, ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90169-u
更新日期：1990-07-01 00:00:00
abstract：:Mitochondrial damage and epigenetic modifications have been implicated in the pathogenesis of Age-related Macular Degeneration (AMD). This study was designed to investigate the effects of AMD/normal mitochondria on epigenetic regulation in human transmitochondrial retinal pigment epithelial (RPE) cells in vitro. Human...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2019.107701
更新日期：2019-12-01 00:00:00
abstract：:(O-acyl) ω-hydroxy fatty acids (OAHFAs) are a recently found group of polar lipids in meibum. Since these lipids can potentially serve as a surfactant in the tear film lipid layer, the surface properties of a molecule of this lipid class was investigated and compared with a structurally related wax ester and a fatty a...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2013.06.016
更新日期：2013-10-01 00:00:00
abstract：:Studies using conventional electron microscopy describe the cytoplasm of lens fiber cells as having essentially an amorphous structure. We hypothesized that significant structural detail might have been lost as a result of projecting the entire thickness of the section (50-100 nm) onto a single plane (the "projection ...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2008.11.029
更新日期：2009-03-01 00:00:00
abstract：:The benzopyran BP (3,4-dihydro-6-hydroxy-7-methoxy-2,2-dimethyl-1(2H)-benzopyran) is a free radical scavenger that is structurally similar to alpha-tocopherol and has provided neuro-protection in a number of disease models where oxidative stress is a causative factor. A novel derivative of BP with improved lipid solub...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/j.exer.2011.04.007
更新日期：2011-07-01 00:00:00
abstract：:Angiotensin II and aldosterone are the main effectors of the renin-angiotensin aldosterone system (RAAS) and have a central role in hypertension as well as cardiovascular and renal disease. The localization of RAAS components within the retina has led to studies investigating the roles of angiotensin II, aldosterone a...
journal_title：Experimental eye research
pub_type： 杂志文章,评审
doi：10.1016/j.exer.2019.107766
更新日期：2019-10-01 00:00:00
abstract：:The effect of a 'soft' anti-inflammatory corticosteroid on the corneal wound healing was investigated in rabbits. Albino rabbits were treated with soft steroid and dexamethasone after linear perforating corneal incision. Soft drug design is aimed to control and direct the drug activity and in vivo metabolism to nontox...
journal_title：Experimental eye research
pub_type： 杂志文章
doi：10.1016/0014-4835(90)90229-n
更新日期：1990-02-01 00:00:00
