Abstract:
:The phosphatidylinositol (PI) and phosphatidylinositol 4,5-bisphosphate (PIP2) hydrolytic activities of phosphoinositide-specific phospholipase C (PLC) were measured in membrane and cytosol fractions from 7 discrete areas of the rat brain. Both the PI-PLC and PIP2-PLC specific activities were found to differ significantly among the 7 discrete brain areas. In the membrane fraction, the PIP2-PLC activity was higher than that of PI-PLC in each region, suggesting that the PLC in membranes prefers PIP2 to PI as substrate. The PIP2-PLC activities in the membrane were high in prefrontal cortex and cerebellum, but rather low in medulla oblongata and hypothalamus. The PI-PLC specific activity in the cytosol was significantly higher than that in the membrane of all brain areas examined. The PI-PLC specific activity in membranes is inversely proportional to its activity in the cytosol. In the cytosol fraction, the distribution pattern of PI-PLC specific activity resembled that of PIP2-PLC. These results indicate that PLCs are differently distributed in various regions of rat brain, and suggest the regional differences in neuronal transduction.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Nishida A,Kaiya H,Tohmatsu T,Banno Y,Wakabayashi S,Nozawa Ydoi
10.1016/0304-3940(88)90215-7subject
Has Abstractpub_date
1988-08-01 00:00:00pages
354-7issue
3eissn
0304-3940issn
1872-7972pii
0304-3940(88)90215-7journal_volume
90pub_type
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