Abstract:
:Although the therapeutic use of microglia has received some attention for the treatment of brain diseases, few non-invasive techniques exist for monitoring the cells after administration. Here, we present a technique using magnetic resonance imaging (MRI) to track microglia injected intra-cardially. We labeled microglia expressing enhanced green fluorescent protein with superparamagnetic iron oxide (Resovist) using the hemagglutinating virus of Japan-envelope vector. We injected labeled microglia into the left ventricle of the heart of mice. After monitoring exogenously administered microglia in the mouse brain in vivo using T(2)*-weighted MRI at a magnetic field of 7T, we compared the MR images with histochemical localization of exogenous microglia in vitro. MRI revealed clear signal changes attributable to Resovist-containing microglia in the mouse brain. Histochemistry demonstrated the presence of exogenous microglia in the brain at the same locations shown by MRI. This study demonstrates the usefulness of MRI for non-invasive monitoring of exogenous microglia, and suggests a promising future for microglia/macrophages as therapeutic tools for brain disease.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Song Y,Morikawa S,Morita M,Inubushi T,Takada T,Torii R,Tooyama Idoi
10.1016/j.neulet.2005.10.074keywords:
subject
Has Abstractpub_date
2006-02-27 00:00:00pages
42-5issue
1eissn
0304-3940issn
1872-7972pii
S0304-3940(05)01237-1journal_volume
395pub_type
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