Abstract：
:A method is developed for PCR assay of mRNA using 28S rRNA as internal standard and its applicability is reported for 4 types of mRNA. It consists of reverse transcription of total RNA using primers selective for 28S rRNA and the message of interest. Routinely the 28S reaction mix is diluted 20,000-fold and the dilution error is minimized by adding 10 microCi of 3H2O before the dilution and determining the level of 3H before and after the dilution. Aliquots from the diluted 28S mix and the undiluted reverse transcripts are co-amplified by PCR in the presence of 32P-dATP. The samples are analyzed by electrophoresis, bands detected by autoradiography and in each lane the ratio is determined between cpm in the band of interest and cpm in the 28S band. The ratio is a measure of the level of mRNA in the sample and can be used to monitor changes in mRNA during treatments, development and pathogenesis.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Khan I,Tabb T,Garfield RE,Grover AKdoi
10.1016/0304-3940(92)90787-8keywords:
subject
Has Abstractpub_date
1992-11-23 00:00:00pages
114-7issue
1eissn
0304-3940issn
1872-7972pii
0304-3940(92)90787-8journal_volume
147pub_type
杂志文章abstract：:The electrophysiological mechanism underlying afterhyperpolarization induced by the activation of the nicotinic acetylcholine receptor (nAChR) in male rat major pelvic ganglion neurons (MPG) was investigated using a gramicidin-perforated patch clamp and microscopic fluorescence measurement system. Acetylcholine (ACh) ...
journal_title：Neuroscience letters
pub_type： 杂志文章
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更新日期：2010-09-27 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)11231-7
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abstract：:Occurrence of neurocalcin, a calcium-binding protein with three EF hand motifs, was examined immunohistochemically in the rat olfactory epithelium and vomeronasal organ epithelium. Immunoreactivity was detected in receptor cells in these epithelia. Immunoreactions were distributed in cytoplasm associated with outer mi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)11568-2
更新日期：1995-05-19 00:00:00
abstract：:Complement is implicated in the pathogenesis of inflammatory disorders of the central nervous system (CNS), like multiple sclerosis, Alzheimer's disease, and trauma. The anaphylatoxins C3a and C5a are thought to be the major contributors to complement-mediated inflammation in the CNS, likely mediating their effects vi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.07.015
更新日期：2005-10-21 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2020.135300
更新日期：2020-09-25 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2003.09.020
更新日期：2004-01-02 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(82)90170-7
更新日期：1982-12-30 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90433-3
更新日期：1989-06-05 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
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更新日期：1984-11-23 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00382-1
更新日期：1999-06-18 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2009.01.002
更新日期：2009-02-27 00:00:00
abstract：:Using in vivo voltammetric detection of nitric oxide (NO) a previous study demonstrated an increased NO release at the lumbar dorsal horn level of the spinal cord by peripheral inflammatory processes in decerebrated-spinalized rats. This study concerns the effects of acute peripheral stimulations. Gentle non-noxious o...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(02)00009-5
更新日期：2002-03-01 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(92)90828-u
更新日期：1992-12-14 00:00:00
abstract：:As the pre-dementia phase of Alzheimer disease, mild cognitive impairment (MCI) involves the onset and development of cognitive impairments. Opioid receptors play pivotal roles in the regulation of learning and cognition. Our study focused on the association of OPRK1 and OPRM1 methylation with MCI in Xinjiang Uygur an...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2016.11.018
更新日期：2017-01-01 00:00:00
abstract：:Deep brain stimulation (DBS) of the anterior limb of the internal capsule (ALIC) may be effective in treating depression. Parental verbal abuse has been linked to decreased fractional anisotropy (FA) of white matter and reduced FA correlated with depression and anxiety scores. Utilizing a nonhuman primate model of moo...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.06.012
更新日期：2010-08-16 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)12042-3
更新日期：1995-10-20 00:00:00
abstract：:N-Ethylmaleimide sensitive factor (NSF) is a presynaptic protein that has been suggested to be differentially expressed in the cortex of schizophrenic subjects through both high-throughput proteomic and genomic screening studies. Thus, to expand upon these studies we measured NSF using Western blotting in four regions...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.08.051
更新日期：2006-01-02 00:00:00
abstract：:Mental activity which engages one half of the brain is usually accompanied by a suppression of alpha rhythm that is greater over the active hemisphere. When the left hemisphere is active, as during mental arithmetic, the magnitude of the asymmetry in the alpha rhythm is proportional to the rate at which subjects perfo...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(77)90144-6
更新日期：1977-03-01 00:00:00
abstract：:The fine-tuning of topographically organized projections in sensory systems is strongly influenced by electrical activity and use-dependent modifications in synaptic strength. Since calcineurin (CaN), a Ca(2+)-calmodulin dependent serine/threonine phosphatase has been associated with activity-dependent modifications i...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.09.071
更新日期：2011-01-03 00:00:00
abstract：:We have previously shown that P7 rat pups injected with the N-methyl-d-aspartate receptor (NMDAR) blocker MK801 displayed robust apoptotic injury within hours after injection. Further studies from our lab suggest that loss of calcium cannot be compensated for when vulnerable neurons lack calcium buffering capabilities...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.12.002
更新日期：2009-02-06 00:00:00
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journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)11991-5
更新日期：1995-09-29 00:00:00
abstract：:Tourette syndrome/chronic tic phenotype (TS-CTD) is a neurological disorder manifested particularly by motor and vocal tics and associated with a variety of behavioral abnormalities. Recently, the mitochondrial ribosomal protein L3 gene (MRPL3) S75N, the DnaJ (Hsp40) homolog subfamily C member 13 gene (DNAJC13) A2057S...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.03.097
更新日期：2012-05-23 00:00:00
abstract：:Ideomotor theory states that the neural codes that represent action and the perceptual consequences of those actions are tightly bound in a common code. For action imagination, bound action, and perceptual codes are thought to be internally activated at a sub-threshold level through action simulation. In support of th...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2014.11.021
更新日期：2015-01-12 00:00:00
abstract：:Galanin (GAL) is distributed in sympathetic nerves in the cat, and exogenous GAL inhibits cardiac vagal action and lowers blood pressure in this species. This study on anaesthetised cats compares the effects on cardiac vagal action and blood pressure of human, rat and porcine GAL. Human GAL has only recently been sequ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(92)90659-u
更新日期：1992-02-17 00:00:00
abstract：:This study compared the immediate and prolonged effects of acute bouts of active and passive leg cycling on the soleus H-reflex among 12 young healthy men. The soleus H-reflex was measured immediately before and then 10 and 30 min after 20 min of active, unloaded leg cycling, passively induced leg cycling, and quiet r...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(03)00652-9
更新日期：2003-08-21 00:00:00
abstract：:Lowering extracellular calcium concentration [Ca2+]o in rat hippocampal slices can lead to an induction of epileptiform activity. It has been shown that this effect is more pronounced in slices of neonatal rats (postnatal day, PND 8-19) than in mature slices (> PND 40) and it has been suggested that unique N-methyl-D-...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)11748-l
更新日期：1995-07-21 00:00:00
abstract：:NCB-20 cells expressed m1- and m4-muscarinic acetylcholine receptor (mAChR) mRNAs, while NG108-15 cells expressed only m4-mAChR mRNA. Butyrate induced a time-dependent increase in the level of m1-mAChR mRNA with no change in the m4-mAChR mRNA level in NCB-20 cells. Similarly, butyrate did not affect the m4-mAChR mRNA ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(96)12779-8
更新日期：1996-07-05 00:00:00
abstract：:Recently, it has been hypothesized that the N-terminal portion of substance P (SP), SP(1-7), which results from the action of endopeptidase 24.11 (EC3.4.24.11), could be involved in mediating the depressor effects of baroreceptor afferent activation via its action on cells in the nucleus tractus solitarius (NTS). In t...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(90)90117-r
更新日期：1990-09-04 00:00:00
abstract：:Personalized or precision medicine is a medical discipline that proposes tailoring health care to each individual by integrating data from their genetic makeup, epigenetic modifications, other biomarkers, clinical symptoms and environmental exposures. Currently, patients typically present for treatment of mood disorde...
journal_title：Neuroscience letters
pub_type： 杂志文章,评审
doi：10.1016/j.neulet.2016.09.049
更新日期：2018-03-16 00:00:00
abstract：:The hippocampus is a brain structure specifically vulnerable to short periods of transient cerebral ischemia, and which displays delayed neuronal necrosis. Protein ubiquitination is a posttranslational modification of proteins and an important factor in heat shock response and a regulator of ATP-dependent protein degr...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(89)90389-3
更新日期：1989-01-30 00:00:00
