Differential effects of butyrate and dibutyryl cAMP on mRNA levels of muscarinic acetylcholine receptor subtypes expressed in neurohybrid cell lines.

Abstract:

:NCB-20 cells expressed m1- and m4-muscarinic acetylcholine receptor (mAChR) mRNAs, while NG108-15 cells expressed only m4-mAChR mRNA. Butyrate induced a time-dependent increase in the level of m1-mAChR mRNA with no change in the m4-mAChR mRNA level in NCB-20 cells. Similarly, butyrate did not affect the m4-mAChR mRNA level in NG108-15 cells. In contrast, dibutyryl cAMP caused a significant time-dependent decrease in the level of m4-mAChR mRNA in NCB-20 and NG108-15 cells as well as m1-mAChR mRNA in NCB-20 cells. Our results suggest that these two differentiating agents are important physiological regulators of the transcription and/or stability of the mRNA of certain mAChR subtypes expressed in these two neurohybrid cell lines.

journal_name

Neurosci Lett

journal_title

Neuroscience letters

authors

Fukamauchi F,Mataga N,Wang YJ,Chuang DM

doi

10.1016/0304-3940(96)12779-8

subject

Has Abstract

pub_date

1996-07-05 00:00:00

pages

49-52

issue

1

eissn

0304-3940

issn

1872-7972

pii

0304394096127798

journal_volume

212

pub_type

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