Abstract：
BACKGROUND:The proepicardium (PE), a transient embryonic structure crucial for the development of the epicardium and heart, contains its own population of endothelial cells (ECs). The aim of our study was to determine the pattern, anatomical orientation and phenotypic marker expression of the endothelial cell network within the PE. RESULTS:Immunohistochemical findings revealed that proepicardial ECs express both early and late EC-specific markers such as CD31, Flk-1, Lyve-1 and Tie-2 but not SCL/Tal1, vWF, Dll4 or Notch1. Proepicardial ECs are present in the vicinity of the sinus venosus (SV) and form a continuous network of vascular sprouts/tubules connected with the SV endothelium, with Ter-119-positive erythroblasts in the vascular lumina. CONCLUSIONS:On the basis of our results, we postulate the existence of a continuous network of ECs in the PE, exhibiting connection and/or patency with the SV and forming vessels/tubules/strands. Marker expression suggests that ECs are immature and undifferentiated, which was also confirmed with a transmission electron microscopy (TEM) analysis. Our results deliver new data for a better understanding of the nature of proepicardial ECs.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Niderla-Bielińska J,Gula G,Flaht-Zabost A,Jankowska-Steifer E,Czarnowska E,Radomska-Leśniewska DM,Ciszek B,Ratajska Adoi
10.1016/j.mvr.2015.08.007subject
Has Abstractpub_date
2015-11-01 00:00:00pages
54-69eissn
0026-2862issn
1095-9319pii
S0026-2862(15)30021-2journal_volume
102pub_type
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更新日期：2008-04-01 00:00:00
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更新日期：2005-07-01 00:00:00
