Abstract：
:Fluorescence-mediated photoplethysmography (FM-PPG) was developed to facilitate determination of tissue microvascular (capillary) blood perfusion, requiring intra-venous injection of a small bolus (~0.33mL) of dye (25mg/mL ICG) and acquisition of high-speed angiogram images (>15images/s). The methodology is applicable to microvascular capillary beds of tissues that can be optically imaged, directly or laparoscopically. Proof of concept of the methodology and feasibility of its implementation are demonstrated in human forearm skin and in the choroid of a monkey eye, determined to have blood perfusion rates on the order of 1e-5mL/(s·mm2) and 10e-5mL/(s·mm2), respectively. Ability to obtain absolute quantified tissue perfusion data ultimately can provide the means by which to characterize blood flow information at the nutritive capillary-vessel level in an objective and universally understood manner, in much the same sense that body temperature or blood pressure are.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Flower RW,Kling Rdoi
10.1016/j.mvr.2017.05.009subject
Has Abstractpub_date
2017-11-01 00:00:00pages
92-100eissn
0026-2862issn
1095-9319pii
S0026-2862(16)30109-1journal_volume
114pub_type
杂志文章abstract：:Growing interest in utilizing endothelial cells (ECs) for therapeutic purposes has led to the exploration of human embryonic stem cells (hESCs) as a potential source for endothelial progenitors. In this study, ECs were induced from hESC lines and their biological characteristics were analyzed and compared with both co...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2012.06.003
更新日期：2012-09-01 00:00:00
abstract：:Controversy exists concerning whether activation of N-methyl-D-aspartate (NMDA) receptors exerts direct dilator effects on cerebral arteries. The purpose of this study was to examine the responses of isolated piglet arteries to NMDA to determine whether isolated arteries, apart from surrounding neuronal tissue, are ca...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2005.04.007
更新日期：2005-07-01 00:00:00
abstract：AIMS:The purpose of the present study was to investigate the possible role of TIPE2 on acute lung injury (ALI) induced by myocardial ischemia/reperfusion (MIR) in diabetic rats. METHODS:Sprague-Dawley (SD) rats were randomly separated into four groups: control+sham (C + sham); control+MIR (C + MIR); diabetes+sham (D +...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2020.104009
更新日期：2020-07-01 00:00:00
abstract：:Living mesenteric capillaries with either an intact or disrupted glycocalyx were challenged with ramp change in shear stress (Deltatau). Animals (Rana pipiens) were divided randomly into two experimental groups, and two true capillaries (TC) per animal were investigated. The modified Landis technique was combined with...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.12.001
更新日期：2010-03-01 00:00:00
abstract：:The stereomorphological arrangement of skeletal muscle capillaries depends on many factors. Genetic biochemical composition of muscle fibers, age, and training can interfere with the growth of the capillary network. This study is focused on the age effect of chronic reduction of blood flow, as in arterial stenosis, in...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(84)90060-8
更新日期：1984-05-01 00:00:00
abstract：UNLABELLED:Metabolic syndrome (MetS) increases the risk of the subsequent development of cardiovascular disease. This study aimed to determine if the harmonic indexes of finger photoplethysmography (PPG) waveforms can be used to discriminate different arterial pulse transmission conditions between MetS and healthy subj...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2016.04.001
更新日期：2016-07-01 00:00:00
abstract：:Laser Doppler flowmetry (LDF) of forearm skin blood flow, combined with iontophoretically-administered acetylcholine and sodium nitroprusside and wavelet spectral analysis, was used for noninvasive evaluation of endothelial function in 17 patients newly diagnosed with New York Heart Association class II-III congestive...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2008.07.006
更新日期：2008-11-01 00:00:00
abstract：:Systemic sclerosis (SSc) is a connective tissue disease with high morbidity and mortality, characterized by autoimmunity, obliterative vasculopathy involving mainly the microvasculature, and fibrosis. SSc-specific nailfold capillaroscopic changes have been defined, and nailfold capillaroscopy (NFC) is now unequivocall...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2018.03.004
更新日期：2018-07-01 00:00:00
abstract：:Arterioles in various vascular beds have been often observed to have nonuniform diameters along the vessel axis. The effect of nonuniformity of arteriolar diameter on vascular resistance was investigated using a theoretical model of blood flow in arterioles. Viscous flow of a Newtonian fluid in tubes with periodically...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(89)90023-x
更新日期：1989-09-01 00:00:00
abstract：:It is generally assumed that when a muscle is shortened or extended the total length of capillaries does not change, implying that capillaries are nondistensible, longitudinally. On the basis of stereological estimates of capillary anisotropy versus sarcomere length, we propose that as long as capillaries are in a tor...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(90)90008-f
更新日期：1990-07-01 00:00:00
abstract：:In preeclampsia, invasion of intrauterine decidual blood vessels by placental cytotrophoblasts is significantly reduced. This study examined the secretion of matrix metalloproteinases (MMP) by cultured human decidual endothelial cells from normal (NDEC) and preeclamptic (PEDEC) pregnancies. MMPs secreted into the cult...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1998.2142
更新日期：1999-05-01 00:00:00
abstract：:We examined the impact of lymph flow obstruction in large post-nodal lymphatic vessels in sheep. A silk ligature was placed 2 cm downstream from the prescapular or popliteal lymph node and tightened to interrupt flow. At 6, 12 and 16 weeks after lymph flow blockage, a network of small interconnecting lymphatics (appro...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2006.11.003
更新日期：2007-05-01 00:00:00
abstract：:The anatomic arrangement, pressure distribution, and resting vascular tone of the feed arteries located upstream from the rat cremaster microcirculation were determined to characterize the sites of the vascular resistance in this macrovessel segment of the cremaster circulation. The cremaster microcirculation and its ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(87)90009-4
更新日期：1987-01-01 00:00:00
abstract：:Purine nucleosides and nucleobases play key roles in the physiological response to vascular ischemia/reperfusion events. The intra- and extracellular concentrations of these compounds are controlled, in part, by equilibrative nucleoside transporter subtype 1 (ENT1; SLC29A1) and by equilibrative nucleobase transporter ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.06.008
更新日期：2014-09-01 00:00:00
abstract：:Polymorphonuclear leukocytes are thought to play a pivotal role in the generation of ischemia-reperfusion-induced mucosal injury; however, their behavior in the intestinal microvasculature following this injury has not been directly examined. In this study intravital microscopy was used to investigate the dynamics of ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1028
更新日期：1994-05-01 00:00:00
abstract：:Red blood cell (RBC)-plasma suspensions with a hematocrit of 10-12% were forced to flow through microchannels (equivalent diameter 6 microns, equivalent length 20 microns, 2600 in parallel) by applying a pressure difference of 20 cm H2O. Transit times for 100 microliters of fresh suspensions from 33 healthy male subje...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1017
更新日期：1994-03-01 00:00:00
abstract：:The aim of this study is to develop a novel non-contrast 4-dimensional MR arterial spin labeling (4D-ASL) technique (3D acquisition and time) and to investigate myocardial perfusion on healthy volunteers without administration of contrast materials. A non-contrast 4D-ASL technique was developed using the time-spatial ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.01.007
更新日期：2015-03-01 00:00:00
abstract：:The distribution of anionic sites in the basal laminae of the blood capillaries of the murine pancreas was studied in specimens fixed in ruthenium red (RR)-glutaraldehyde mixtures. The sites appeared as discrete, small (6 to 18 nm) particles distributed throughout the three laminae but concentrated primarily in the la...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(84)90006-2
更新日期：1984-11-01 00:00:00
abstract：:During diabetes in rats, the choroid of the eye shows increased permeability to albumin, basement membrane thickening, and decreased anionic charge sites on the abluminal surfaces of the choriocapillary microvessels. In other microvascular beds, permeability differences are correlated with differences in luminal membr...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(91)90058-j
更新日期：1991-11-01 00:00:00
abstract：:The most universal angiogenic cytokines (VEGF, bFGF, HGF) are all heparin-binding proteins, the function of which is dependent on cell surface heparan sulfate proteoglycans (HSPG). Several proteoglycans have been demonstrated in endothelial cells, but only glypican-1 from the cell surface HSPG subfamily was documented...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2004.05.001
更新日期：2004-09-01 00:00:00
abstract：:Pigment epithelium-derived factor (PEDF) is a potent inhibitor of angiogenesis in the mammalian eye, suggesting that loss of PEDF is implicated in the pathogenesis of proliferative diabetic retinopathy. However, a role for PEDF in early diabetic retinopathy remains to be elucidated. Since oxidative stress is thought t...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2004.11.001
更新日期：2005-01-01 00:00:00
abstract：OBJECTIVE:High aerobic fitness may prevent age-related decrements in cutaneous vasodilation while type 2 diabetes may exacerbate this decline. The mechanisms underlying these responses remain unclear, but may be due to an excess of reactive oxygen species. We hypothesized that superoxide scavenging or NADPH oxidase inh...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.103886
更新日期：2019-09-01 00:00:00
abstract：:Diabetic retinopathy (DR) is a disease that causes blindness due to vascular leakage or abnormal angiogenesis. Hepatocyte growth factor (HGF) is increased in the serum or vitreous fluid in proliferative diabetic retinopathy (PDR) patients, although the effect of HGF on the blood vessels remains unclear. This study foc...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2020.104103
更新日期：2021-01-01 00:00:00
abstract：:A microvascular network model described in the preceding paper (B. Dawant, M. Levin, and A. S. Popel, 1986, Microvasc. Res. 31, 203-222) featuring random distribution of vessel diameters and lengths, is extended to calculate the distributions of red blood cell flux and discharge hematocrit throughout the network. A re...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(86)90036-1
更新日期：1986-03-01 00:00:00
abstract：:We examined the influence of local cooling (-15 degrees C, 60 sec) on blood flow velocity in nailfold capillaries and on laser Doppler flux (LDF) at the fingertip in 12 patients with primary Raynaud's phenomenon (PRP) and 13 patients with secondary Raynaud's (SRP) in comparison with 10 health controls. Skin temperatur...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1993.1060
更新日期：1993-11-01 00:00:00
abstract：:Microvascular dysfunction contributes to the development of cardiovascular and metabolic disease. This study tested the hypothesis that young obese (BMI>30 kg m(-2)), otherwise healthy, adults (N=15) have impaired microvascular function relative to age and sex matched, lean (BMI<25 kg m(-2)) individuals (N=14). Partic...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.11.007
更新日期：2016-03-01 00:00:00
abstract：:Pulmonary arterial hypertension is a common complication of congenital heart defects with left-to-right shunts. Current preclinical models do not reproduce clinical characteristics of shunt-related pulmonary hypertension. Aorto-caval shunt was firstly described as a model of right ventricle volume overload. The pathop...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.05.005
更新日期：2014-07-01 00:00:00
abstract：:Since the internal carotid artery supplies blood to both the eye and the brain, ocular microvascular hemodynamics can be altered due to ischemic stroke. The purpose of the current study was to establish the feasibility of conjunctival microcirculation imaging for detection of inter-ocular differences in microvascular ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.04.007
更新日期：2015-07-01 00:00:00
abstract：:In this study cerebral capillary bed structure and the effects of chronic hypertension on these systems have been assessed in 6- to 7-month-old spontaneously hypertensive (SHR) and Wistar-Kyoto (WKY) rats. Capillary diameter (D), profile frequency (Na), volume fraction (Vv), and surface area (Sv) were quantitated by l...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(90)90032-m
更新日期：1990-11-01 00:00:00
abstract：:Skeletal muscle arterioles are known to constrict upon elevation of ambient PO2. While several studies have shown that the endothelium plays an important role in this response, it is not clear how this response is mediated. We examined the oxygen-induced constriction of arterioles in the rat spinotrapezius muscle. Ele...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1995.1025
更新日期：1995-05-01 00:00:00
