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Expression of CD44v3 protein in human endothelial cells in vitro and in tumoral microvessels in vivo.

Abstract：

:The most universal angiogenic cytokines (VEGF, bFGF, HGF) are all heparin-binding proteins, the function of which is dependent on cell surface heparan sulfate proteoglycans (HSPG). Several proteoglycans have been demonstrated in endothelial cells, but only glypican-1 from the cell surface HSPG subfamily was documented at protein level. Here, we show that CD44v3 is expressed in human immortalized endothelial cells [anchorage-dependent human umbilical vein endothelial cells (HUVEC) and anchorage-independent Kaposi sarcoma (KS-Imm)] at mRNA and protein level, but is absent from the primary culture of human brain microvascular endothelial cells. We have shown that CD44v3 has a large cytoplasmic pool in endothelial cells, but a limited surface expression, mainly at filopodia, colocalized with MMP-2. Angiogenic factors like VEGF or bFGF did not affect surface detection of CD44v3 suggesting a constitutive expression. The putative functional role for endothelial cell surface CD44v3 was identified in chemotaxis assay when anti-CD44v3 antibody pretreatment proved to be inhibitory for HUVEC. Furthermore, we provided evidence for the CD44v3 protein expression in human endothelial cells in vivo in peritumoral microvessels of both human melanoma and glottic cancers, suggesting a role for this part-time heparan sulfate proteoglycan in tumor induced angiogenesis.

journal_name

Microvasc Res

journal_title

Microvascular research

authors

Forster-Horváth C,Mészáros L,Rásó E,Döme B,Ladányi A,Morini M,Albini A,Tímár J

doi

10.1016/j.mvr.2004.05.001

keywords:

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

110-8

issue

2

eissn

0026-2862

issn

1095-9319

pii

S0026286204000524

journal_volume

68

pub_type

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