Abstract：
:Living mesenteric capillaries with either an intact or disrupted glycocalyx were challenged with ramp change in shear stress (Deltatau). Animals (Rana pipiens) were divided randomly into two experimental groups, and two true capillaries (TC) per animal were investigated. The modified Landis technique was combined with intravital microscopy to view individual TC and assess hydraulic conductivity (L(p)), an index of capillary function. Median L(p) was 3.2 x 10(-7) for control and 11.8 x 10(-7) cm s(-1) cm H(2)O(-1) after mild, brief (1 min) pronase treatment (P<0.0001). Analysis by stimulus component showed that L(p) for untreated capillaries was related negatively to ramp acceleration (R(2)=0.46, P<0.0001, n=38) and positively to Deltatau magnitude (R(2)=0.28, P=0.0006, n=38). Disrupting the capillary glycocalyx revealed a positive and previously unknown relationship between ramp acceleration and L(p) (R(2)=0.44, P=0.002, n=19) plus an upward shift (increased intercept) of the magnitude Deltatau-L(p) relationship compared to abrupt stimulation. These data suggest that bloodstream hemodynamics may impact capillary function. Further, an intact glycocalyx may protect capillaries when blood flow changes.
journal_name
Microvasc Resjournal_title
Microvascular researchauthors
Williams DAdoi
10.1016/j.mvr.2009.12.001subject
Has Abstractpub_date
2010-03-01 00:00:00pages
114-20issue
2eissn
0026-2862issn
1095-9319pii
S0026-2862(09)00284-2journal_volume
79pub_type
杂志文章abstract：:Controversy exists concerning whether activation of N-methyl-D-aspartate (NMDA) receptors exerts direct dilator effects on cerebral arteries. The purpose of this study was to examine the responses of isolated piglet arteries to NMDA to determine whether isolated arteries, apart from surrounding neuronal tissue, are ca...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2005.04.007
更新日期：2005-07-01 00:00:00
abstract：:The effect of isoprenaline infusion (1 gamma/min) on blood flow in the extremities was studied in 15 Raynaud's disease (RD) patients and 9 healthy controls. A highly significant (P less than 0.0005) decrease in blood flow was seen in the RD subjects. It is suggested that an increased presynaptic beta receptor response...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(84)90045-1
更新日期：1984-01-01 00:00:00
abstract：:The present study was conducted to compare laser speckle imaging (LSI) with sidestream dark field (SDF) imaging (i.e., capillary microscopy) so as to validate the use of LSI for assessing microvascular (re)perfusion. For this purpose, LSI and SDF measurements were performed on the human nail fold during gradual occlus...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.01.004
更新日期：2010-03-01 00:00:00
abstract：:The extravascular uptake for labeled albumin and IgG and the extravascular masses for endogenous albumin and IgG were measured in skin and skeletal muscle from anesthetized rabbits following 24 hr of intermittent plasmapheresis. An amount of protein equivalent to the total intravascular protein mass was removed. There...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(92)90089-8
更新日期：1992-11-01 00:00:00
abstract：:It has generally been assumed that movement of fluid between the pulmonary microvasculature and surrounding tissues is governed by a "Starling" balance of hydrostatic and protein osmotic forces similar to that which prevails in the extremities. However, both recent and older observations suggest that the lungs are mor...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2009.03.004
更新日期：2009-06-01 00:00:00
abstract：:Systemic sclerosis (SSc) is a connective tissue disease with high morbidity and mortality, characterized by autoimmunity, obliterative vasculopathy involving mainly the microvasculature, and fibrosis. SSc-specific nailfold capillaroscopic changes have been defined, and nailfold capillaroscopy (NFC) is now unequivocall...
journal_title：Microvascular research
pub_type： 杂志文章,评审
doi：10.1016/j.mvr.2018.03.004
更新日期：2018-07-01 00:00:00
abstract：:The purpose of this study was to determine whether bidirectional flow exists in the sciatic vasa nervorum. Images obtained using high-frequency color Doppler ultrasound in duplex imaging mode (Vevo 2100) were studied retroactively. In Fig. 1 (left panel; rat 1), the color Doppler signal and flow-velocity waveforms are...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.05.008
更新日期：2014-07-01 00:00:00
abstract：:A hallmark of severe inflammation is reactive oxygen species (ROS) overproduction induced by increased inflammatory mediators secretion. During systemic inflammation, inflammation mediators circulating in the bloodstream interact with endothelial cells (ECs) raising intracellular oxidative stress at the endothelial mo...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2014.02.001
更新日期：2015-03-01 00:00:00
abstract：:Laser Doppler flowmetry (LDF) of forearm skin blood flow, combined with iontophoretically-administered acetylcholine and sodium nitroprusside and wavelet spectral analysis, was used for noninvasive evaluation of endothelial function in 17 patients newly diagnosed with New York Heart Association class II-III congestive...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2008.07.006
更新日期：2008-11-01 00:00:00
abstract：:Lipopolysaccharide released during bacterial sepsis causes acute lung injury and ARDS. Pulmonary microvascular injury is a feature of ARDS, and vascular remodeling develops, leading to pulmonary hypertension. Pericytes in the lung circulation proliferate and contribute to the remodeling seen in experimental sepsis. It...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1998.2085
更新日期：1998-09-01 00:00:00
abstract：:Current tissue engineering techniques have failed to provide an established microvasculature in skin substitutes, a requisite for the maintenance of graft viability and rapid revascularization subsequent to graft transplantation in vivo. To improve outcomes for both conventional skin grafts and skin substitutes, the e...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2007.04.012
更新日期：2008-01-01 00:00:00
abstract：:Oxygen tension was recorded with double-barreled microelectrodes in the retina of anesthetized cats and monkeys. The oxygen tension (PO2) was often observed to undergo spontaneous irregular fluctuations with peak-to-peak amplitudes of about 3 to as much as 20 mm Hg. These fluctuations were observed only in the vascula...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(92)90103-v
更新日期：1992-07-01 00:00:00
abstract：:Overexpression of EGFR plays a key-role in head and neck squamous cell carcinoma (HNSCC) and justifies the extensive use of cetuximab, a monoclonal anti-EGFR antibody, as well as EGFR-tyrosine kinase inhibitors (EGFR-TKI), which have been reported to inhibit tumor cell growth and the secretion of pro-angiogenic factor...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2011.07.008
更新日期：2012-03-01 00:00:00
abstract：:H2O2 mediates autocrine and paracrine signaling in the vasculature and can propagate endothelial dysfunction. However, it is not clear how endothelial cells withstand H2O2 exposure and promote H2O2-induced vascular remodeling. To understand the innate ability of endothelial cells for sustaining excess H2O2 exposure, w...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.09.003
更新日期：2016-01-01 00:00:00
abstract：:A large loss of blood during hemorrhage can result in profound shock, a state of hypotension associated with hemodynamic abnormalities. One of the hypotheses to account for this collapse of homeostasis is that the production of nitric oxide (NO), a gas molecule that dilates blood vessels, is significantly impaired dur...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.02.009
更新日期：2009-06-01 00:00:00
abstract：:Studies of microvascular geometry made from microscope observations of tissues in vivo or after perfusion with a silastic elastomer or india ink are restricted to a two-dimensional field of view. Microvascular corrosion casts, however, if of sufficient rigidity and structural integrity, can yield three-dimensional inf...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(83)90044-4
更新日期：1983-01-01 00:00:00
abstract：:We tested the hypothesis that cationic peptides (polyarginine; poly-argn) and eosinophil-derived major basic protein (MaBP) increase permeability by stimulating the release of histamine and/or nitric oxide. We used intravital microscopy, clearance, and integrated optical intensity (IOI), using FITC-dextran 150 (FITC-d...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1995.1038
更新日期：1995-07-01 00:00:00
abstract：:The purpose of this study was to determine if there are intrinsic differences in resting tone, vascular reactivity, myogenic responses, and neurogenic vasoconstriction between the large and small feeder arteries and first order arterioles (1A) of the rat cremaster muscle. The pudic-epigastric artery (PEA), external sp...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.2000.2259
更新日期：2000-09-01 00:00:00
abstract：:Microvascular dysfunction contributes to the development of cardiovascular and metabolic disease. This study tested the hypothesis that young obese (BMI>30 kg m(-2)), otherwise healthy, adults (N=15) have impaired microvascular function relative to age and sex matched, lean (BMI<25 kg m(-2)) individuals (N=14). Partic...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2015.11.007
更新日期：2016-03-01 00:00:00
abstract：PURPOSE:To investigate conjunctival microvascular responses in patients with mild diabetic retinopathy (MDR) and methylenetetrahydrofolate reductase (MTHFR) polymorphisms (D + PM) after administration of Ocufolin™, a medical food containing 900 μg l-methylfolate (levomefolate calcium or [6S]-5-methyltetrahydrofolic aci...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2020.104066
更新日期：2020-11-01 00:00:00
abstract：:In the present study, human myometrial microvascular endothelial cells (HMMEC) were cocultured with human vascular smooth muscle cells (VSMC) labeled with fluorescent dyes to examine their morphological interaction using confocal laser fluorescent microscopy. HMMEC and VSMC labeled with fluorescent green and red dyes,...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2005.04.008
更新日期：2005-07-01 00:00:00
abstract：PURPOSE:Diabetic retinopathy (DR) is characterized by pro-inflammatory, pro-angiogenic and pro-fibrotic environment during the various stages of the disease progression. Basement membrane changes in the retina and formation of fibrovascular membrane are characteristically seen in DR. In the present study the effect of ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2018.09.001
更新日期：2019-01-01 00:00:00
abstract：:The microcirculation in normal and arthritic juxtaarticular bone was studied in 16 young dogs with carragheenan-induced arthritis of one knee. The regional blood flow was determined by the tissue uptake of intracardially injected 15-microns 141Ce-labeled microspheres, and the microvascular plasma volume was determined...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(90)90020-r
更新日期：1990-09-01 00:00:00
abstract：OBJECTIVE:High aerobic fitness may prevent age-related decrements in cutaneous vasodilation while type 2 diabetes may exacerbate this decline. The mechanisms underlying these responses remain unclear, but may be due to an excess of reactive oxygen species. We hypothesized that superoxide scavenging or NADPH oxidase inh...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2019.103886
更新日期：2019-09-01 00:00:00
abstract：:An intravital microscopic method for quantitative measurement of interstitial concentrations of fluorescent tracers has been applied to the investigation of microvascular permeability in the hamster cheek pouch. Some nanoliters of FITC-dextran mean mol wt (Mw) 20,000, 3000, or sodium fluorescein (Mw 376) were injected...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/0026-2862(86)90009-9
更新日期：1986-01-01 00:00:00
abstract：:Polymorphonuclear leukocytes are thought to play a pivotal role in the generation of ischemia-reperfusion-induced mucosal injury; however, their behavior in the intestinal microvasculature following this injury has not been directly examined. In this study intravital microscopy was used to investigate the dynamics of ...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1006/mvre.1994.1028
更新日期：1994-05-01 00:00:00
abstract：:We investigated the role of nitric oxide synthase (NOS) on regional cutaneous vascular function at rest (thermoneutral conditions) and during the vasodilator response to increased local skin temperature (Tloc). Dorsal forearm and lateral leg sites were instrumented with microdialysis fibers, local heaters, and laser-D...
journal_title：Microvascular research
pub_type： 杂志文章
doi：
更新日期：2013-11-01 00:00:00
abstract：:We explored the role of 20-hydroxy-5Z, 8Z, 11Z, 14Z-eicosatetraenoic acid (20-HETE) in oxygen-induced vasoconstriction in a normal renin form of hypertension [the 1 kidney-1 clip Goldblatt hypertensive rat (1K1C)] and a high renin form of hypertension [the 2 kidney-1 clip Goldblatt hypertensive rat (2K1C)]. A silver c...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2009.09.002
更新日期：2009-12-01 00:00:00
abstract：:Changes in blood vessel diameter can be measured manually, but this is time-consuming and often impractical. For automatic measurement commercial solutions are available, but the proprietary algorithms and their potential shortcomings are not known to the user. We present an approach with a CCD camera for image acquis...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2010.04.004
更新日期：2010-09-01 00:00:00
abstract：:Reperfusion of the ischemic lung is associated with increased pulmonary vascular resistance and reduced alveolar perfusion in conjunction with an inflammatory response. To determine the contribution of platelet-endothelial interactions, we examined effects of pulmonary ischemia-reperfusion (IR) on platelet adhesion an...
journal_title：Microvascular research
pub_type： 杂志文章
doi：10.1016/j.mvr.2003.09.003
更新日期：2004-01-01 00:00:00
